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    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Emphysema is a chronic obstructive pulmonary disease &#40;COPD&#41;&#44; limiting air flow during expiration&#44; and is associated with an abnormal inflammatory response of the lungs to noxious particles or gases &#40;mainly cigarette smoke&#41;&#46; This disease is characterized by a permanent abnormal dilatation of alveolar spaces&#46; The destruction of pulmonary parenchyma impairs alveolar gas exchange&#44; compromising the physical capacity of a patient since it is associated with airflow limitations that are not fully reversible&#46;</p><p id="par0010" class="elsevierStylePara elsevierViewall">No therapy capable of reversing emphysematous tissue lesions is available to date and&#44; in severe chronic stages the only treatment that remains is lung transplantation&#44; representing a procedure with high levels of morbidity and mortality&#46; Emphysema&#44; together with other types of COPD&#44; are responsible for more than 2&#46;5 million deaths every year&#44; representing the fifth leading cause of mortality in the world&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">The severity of its pathology together with the lack of any effective treatment transforms emphysema into a great medical challenge&#46; There is a need for studies aiming to understand the pathogenic cellular mechanisms causing tissue destruction in order to elucidate the disease and to open new therapeutic avenues&#46; Another aggravator is the fact that a considerable variation exists in the course of the disease among different smokers&#46; Only about 15&#37; of smokers develop COPD&#46;<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1&#44;2</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">Animal models represent a fundamental instrument to correlate pre-clinical research with clinical studies&#46; The experimental model allows the detailed investigation of different factors influencing emphysema&#46; These factors include inflammatory cell recruitment&#44; genetic background&#44; abnormal matrix repair&#44; lung cell apoptosis&#44; and misbalance between apoptosis and replenishment of structural cells in the lung&#46; Additional factors are research of potential therapeutic agents and strategies&#44; such as administration of stem cells or different growth factors&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a></p><p id="par0025" class="elsevierStylePara elsevierViewall">Until now there are many experimental models of COPD&#44; each of them with advantages and disadvantages&#46; In reality&#44; a number of approaches have been tried because until now none of them constitutes a model that exactly reproduces all phases of development and clinical features of emphysema or any other abnormalities that make up this clinical entity&#44; the COPD&#46; Other factor that affects the reproducibility of data is the differences in anatomical structure with respect to development&#44; maturation&#44; structural organization of respiratory branches&#44; and constituent cells and vascularization&#46;<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">4</span></a> On the other side&#44; some common characteristics can be observed between animal model and human disease&#46; For example&#44; rodents develop the phenomenon of metaplasia induced by reaction to injury&#44;<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> which is a frequently observed response to an insult in different organs in humans&#44; such as in respiratory tract&#44; urinary bladder&#44; and esophagus&#46;</p><p id="par0030" class="elsevierStylePara elsevierViewall">The first animal model of emphysema was developed more than 40 years ago<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a> and it was evoked in rats by intratracheal instillation of the plant proteinase papain&#46; The experimental models of emphysema are continuously improving and became a research tool for several groups of scientists&#46; These include enzyme airway administration model&#44;<a class="elsevierStyleCrossRefs" href="#bib0035"><span class="elsevierStyleSup">7&#44;8</span></a> tobacco smoking model&#44;<a class="elsevierStyleCrossRefs" href="#bib0045"><span class="elsevierStyleSup">9&#44;10</span></a> apoptosis induced emphysema by intratracheal instillation of activated caspase-3&#44;<a class="elsevierStyleCrossRef" href="#bib0055"><span class="elsevierStyleSup">11</span></a> AIAT deficient animals&#44;<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">12</span></a> natural mutants&#44;<a class="elsevierStyleCrossRef" href="#bib0065"><span class="elsevierStyleSup">13</span></a> knockout mice&#44;<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">14</span></a> and transgenic mice&#46;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">15</span></a> Despite the model chosen all of them present restrictions&#46;<a class="elsevierStyleCrossRefs" href="#bib0080"><span class="elsevierStyleSup">16&#8211;22</span></a></p><p id="par0035" class="elsevierStylePara elsevierViewall">Taking these informations into account&#44; 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elastase&#44; matrix metalloprotease imbalance&#44; apoptosis&#44; and oxidative stress&#44; which are reproduced by elastase administration in animal models&#46;<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">33</span></a> We chose to develop the model in female C57Bl&#47;6 mice based on clinical evidences that point out women are more susceptible to emphysema development than men<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">34</span></a> and on pre-clinical assays that demonstrated the higher susceptibility of female A&#47;J mice to emphysema&#46;<a class="elsevierStyleCrossRef" href="#bib0175"><span class="elsevierStyleSup">35</span></a> The use of an appropriate model of emphysema is essential to investigate relevant questions involved in the pathophysiology of the disease and to test the therapeutic potential of new drugs&#46; Another essential point is the use of methods to evaluate the physiological parameters of respiratory in the emphysema model&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">Evaluation of pulmonary function and measurement of physiological parameters are crucial to studies that investigate new therapeutic strategies&#44; pathogenesis of disease and toxicology&#46; There are invasive and noninvasive pulmonary function tests available which are sensitive in detecting abnormalities in mechanical respiratory&#46; The invasive methodology evolution allowed the evaluation of relevant parameters transpulmonary pressure&#44; expiratory and inspiratory flows&#44; such as pulmonary resistance and compliance&#46; Although these procedures give precise data&#44; they involve invasive surgical procedures<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">23</span></a> &#40;orotracheal intubated animal&#41; and anesthesia that impose non-physiological conditions to experiment that may generate significant artifacts&#44; such as decreased respiratory frequency&#44;<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">24</span></a> besides the risk of death of animals due to the procedure which implies the use of a larger number of animals per experimental group&#46; The non-invasive methodology started in the end of 70s<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">25</span></a> and there are basically two types of plethysmograph&#58; double-chamber and single-chamber whole-body instrument&#46; This noninvasive serial measurements reduced the number of animal per study&#44; but long-term examination is not allowed due to the stress imposed by of the neck collar restrained&#46; Noninvasive techniques to evaluate respiratory parameters in conscious mice are easy&#44; convenient&#44; repetitive and reproducible&#44; appropriate for quick and repeatable screening of respiratory function in a large number of conscious animals&#44;<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">26</span></a> however are not so accurate as the invasive method&#44; and some important data about pulmonary function may be misleading&#46;</p><p id="par0045" class="elsevierStylePara elsevierViewall">This kind of evaluation is essential to reveal information about pulmonary function but does not provide data on the degree of impairment of physical capacity of the animal&#46; L&#252;thje et al&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> demonstrated exercise intolerance by treadmill as a consequence of pulmonary emphysema and its systemic consequence in elastase-induced-emphysema model in transgenic mice&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">Following this rational&#44; we sought to evaluate a different procedure to establish experimental pulmonary emphysema in C57Bl&#47;6 using elastase via tracheal puncture&#46; To prove the efficacy of emphysema induction&#44; we investigated the degree of impairment of physical capacity by the evaluation of respiratory function during exercise testing on treadmill&#44; acquiring data about distance run&#44; exercise time&#44; oxygen consumption&#44; and carbon dioxide production&#46; To evaluate the structure alterations we measured LM&#44; an objective parameter that represents the average distance between alveolar walls&#46; The increase of <span class="elsevierStyleItalic">L</span>m with age is relatively small and it is independent of body size&#46; This measurement is easy to make and is independent of the observer&#46; This measurement is considered to be suitable for detailed studies that aim to correlate structure and function disturbances in lungs&#46;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">28</span></a></p><p id="par0055" class="elsevierStylePara elsevierViewall">By the investigation of functional disturbances and structural alterations associated with elastase-induced emphysema in mice&#44; we aimed to establish an experimental model with high reproducibility and low mortality&#46; We analyzed safety&#44; feasibility and reproducibility of our model applying intratracheally an intermediary dose of elastase to produce the enlargement of alveolar space and its consequences on respiratory function&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Materials and methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Animals</span><p id="par0060" class="elsevierStylePara elsevierViewall">Two-month old female C57Bl&#47;6 mice &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>36&#41;&#44; weighing around 20<span class="elsevierStyleHsp" style=""></span>g&#44; raised and maintained at the animal facilities at the Gon&#231;alo Moniz Research Center&#44; FIOCRUZ&#44; were used in the experiments&#44; and were provided with rodent diet and water <span class="elsevierStyleItalic">ad libitum</span>&#46; All animals were euthanized in a CO<span class="elsevierStyleInf">2</span> chamber&#44; and handled according the NIH guidelines for ethical use of laboratory animals&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Experimental groups</span><p id="par0065" class="elsevierStylePara elsevierViewall">The animals were randomly divided into three groups&#58; &#40;A&#41; control&#58; non-manipulated animals &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>12&#41;&#59; &#40;B&#41; intratracheal elastase&#58; 100<span class="elsevierStyleHsp" style=""></span>&#956;l &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>12&#41;&#59; and &#40;C&#41; intratracheal PBS 100<span class="elsevierStyleHsp" style=""></span>&#956;l &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>12&#41;&#46;</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Emphysema induction</span><p id="par0070" class="elsevierStylePara elsevierViewall">For elastase instillation&#44; mice were anaesthetized with ketamine &#40;Vetbrands&#41; and atropine &#40;Allergan&#41;&#44; intubated orotracheally&#44; and ventilated using a ventilator&#47;respirator &#40;Mini-Vent Small Animal Ventilators&#44; Kent Scientific&#41;&#46; Experimental animals received 2<span class="elsevierStyleHsp" style=""></span>U&#47;100<span class="elsevierStyleHsp" style=""></span>g body weight porcine pancreatic elastase &#40;Sigma&#44; Aldrich&#44; Taufkirchen&#44; Germany&#41; dissolved in 100<span class="elsevierStyleHsp" style=""></span>&#956;l phosphate-buffered saline &#40;PBS&#41;solution or 100<span class="elsevierStyleHsp" style=""></span>&#956;l PBS alone&#46; Control animals were not manipulated&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">The respective solution was administered intratracheally via tubing followed by 200<span class="elsevierStyleHsp" style=""></span>&#956;l of air for an even distribution of the liquid throughout both lungs&#46; The animals were submitted to anterior cervical incision in order to visualize the trachea and make the tracheal puncture to administer one of the solutions&#46; Afterwards&#44; mice were sutured&#44; kept on a warm plate &#40;30<span class="elsevierStyleHsp" style=""></span>&#176;C&#41; until restoration of spontaneous breathing and then were extubated&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Treadmill</span><p id="par0080" class="elsevierStylePara elsevierViewall">A motor-driven treadmill chamber for one animal &#40;LE 8700&#44; Panlab&#44; Barcelona&#44; Spain&#41; was used to exercise the animals&#46; The speed of the treadmill and the intensity in milliamps of the electric shock applied to an stainless steel grid at the rear end of the treadmill were controlled by a potentiometer &#40;LE 8700 treadmill control&#44; Panlab&#41;&#46; Room air was pumped into the chamber at a controlled flow rate &#40;700<span class="elsevierStyleHsp" style=""></span>ml&#47;min&#41; by a chamber air supplier &#40;OXYLET LE 400&#44; Panlab&#41;&#46; Outflow was directed to an oxygen and carbon dioxide analyzer &#40;Oxylet 00&#59; Panlab&#41; to measure consumption of oxygen and production of carbon dioxide&#46; The mean room temperature was maintained at 21<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1<span class="elsevierStyleHsp" style=""></span>&#176;C&#46; After an adaptation period of 40<span class="elsevierStyleHsp" style=""></span>min in the treadmill chamber the mice were exercised at different velocities&#44; starting at 7&#46;2<span class="elsevierStyleHsp" style=""></span>m&#47;min and increasing the velocity 7&#46;2<span class="elsevierStyleHsp" style=""></span>m&#47;min every 10<span class="elsevierStyleHsp" style=""></span>min&#46; The inclination of the treadmill was maintained at an uphill angle of 10&#176;&#46; Velocities were increased until the animal could no longer sustain a given speed and remained for more than ten seconds on the electrified grid&#44; which provided an electrical stimulus &#40;1 milliamp&#41; to keep the mice running&#46; Total running distance and running time were recorded&#46; Treadmill tests were carried out on all mice before the induction of emphysema and 28 days after experimental procedures&#46;</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Histopathological analysis</span><p id="par0085" class="elsevierStylePara elsevierViewall">Twenty-eight days after application of elastase or PBS&#44; the mice were euthanized using CO<span class="elsevierStyleInf">2</span> and the lungs were exposed&#46; The trachea was canullated with gelco number 18 and the lungs were perfused with buffered 4&#37; formalin applying a constant transpulmonary pressure of 20<span class="elsevierStyleHsp" style=""></span>cm H<span class="elsevierStyleInf">2</span>O for 2<span class="elsevierStyleHsp" style=""></span>h&#46; After this procedure the trachea was sutured in order to hold the intrapulmonary pressure at 20<span class="elsevierStyleHsp" style=""></span>cm H<span class="elsevierStyleInf">2</span>O and the entire cardiopulmonary tissue block was removed and fixed in formalin &#40;4&#37;&#41;&#46;</p><p id="par0090" class="elsevierStylePara elsevierViewall">Morphologic examinations were performed following Thurlbeck<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">28</span></a> method modified&#46; Briefly&#44; the mean linear intercept &#40;<span class="elsevierStyleItalic">L</span>m&#41; of each lung was determined using light microscopy on 20 randomly selected fields&#44; originating from randomly selected tissue samples covering the entire lung and containing apical as well as basal areas of the organ&#46; Five pictures were taken of each lung&#46; The <span class="elsevierStyleItalic">L</span>m as indicator of air space size was calculated from counting lines of defined length that were randomly placed on each of the 20 lung sections of 5<span class="elsevierStyleHsp" style=""></span>&#956;m and the number of intercepts crossing the lines counted&#46; The <span class="elsevierStyleItalic">L</span>m was calculated from the length of the lines multiplied by the number of the lines divided by the sum of all counted intercepts&#46;</p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Statistical analysis</span><p id="par0095" class="elsevierStylePara elsevierViewall">Numeric values were expressed as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SD unless stated otherwise&#46; Treadmill performance was analysed using a paired <span class="elsevierStyleItalic">t</span>-test to compare groups before and after treatment and morphometric data were compared applying a One-way ANOVA followed by a Tukey test to identify groups that are significantly different&#46; A value of <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05 was considered statistically significant&#46;</p></span></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Results</span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Safety of the procedure &#8211; mortality</span><p id="par0100" class="elsevierStylePara elsevierViewall">Four out of 36 mice &#40;11&#46;1&#37;&#41; died during the experimental procedure&#46; The groups manipulated by intratracheal function showed a similar mortality &#40;two death per group&#41;&#44; independent of the kind of solution administrated &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Functional analysis &#8211; ergometry</span><p id="par0105" class="elsevierStylePara elsevierViewall">Maximum velocity and running distance reached by each group before &#40;pre&#41; and after &#40;post&#41; administration of PBS or elastase are summarized in <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#46;</p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><p id="par0110" class="elsevierStylePara elsevierViewall">Before the manipulation&#44; all mice were capable of exercising on the treadmill&#46; After manipulation&#44; intratracheal elastase administration &#40;group B&#41; showed a significant decrease in the maximum exercise velocity being able to sustain treadmill velocities up to 15&#46;1<span class="elsevierStyleHsp" style=""></span>m&#47;min &#40;<a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46; These differences resulted in a significant reduction in distance covered by tracheal elastase-treated mice when compared to tracheal saline-treated mice&#44; reducing running distance by 30&#46;5&#37; in the former group &#40;<a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46;</p><p id="par0115" class="elsevierStylePara elsevierViewall">Concerning oxygen consumption and carbon dioxide production&#44; all mice treated with elastase increased oxygen consumption after induction when compared at the same speed before and after administration of elastase &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>A&#41;&#46; In relation to the production of carbon dioxide&#44; no significant difference was observed at the same speed before and after administration of elastase &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>B&#41;&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Morphometric analysis &#8211; mean linear interseptum measurement</span><p id="par0120" class="elsevierStylePara elsevierViewall">The administration of 100<span class="elsevierStyleHsp" style=""></span>&#956;l of elastase by the tracheal route significantly increased <span class="elsevierStyleItalic">L</span>m &#40;99&#46;6<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2&#46;9<span class="elsevierStyleHsp" style=""></span>&#956;m&#41; when compared to intratracheal saline-treated &#40;33&#46;3<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;6<span class="elsevierStyleHsp" style=""></span>&#956;m&#41; and non-manipulated mice &#40;25&#46;6<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;1<span class="elsevierStyleHsp" style=""></span>&#956;m&#41;&#46; A slight&#44; but significant&#44; increase in <span class="elsevierStyleItalic">L</span>m was found comparing traqueal application of saline with non-manipulated animals &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41;&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia></span></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Discussion and conclusion</span><p id="par0125" class="elsevierStylePara elsevierViewall">In this work we induced emphysema by intratracheal instillation of porcine pancreatic elastase&#44; which resulted in a mean linear intercept of 99&#46;6<span class="elsevierStyleHsp" style=""></span>&#956;m&#46; Ishizawa et al&#46;<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">29</span></a> applied the same concentration of elastase by intranasal instillation to induce the experimental emphysema in mice and&#44; after three weeks&#44; they observed pulmonary abnormalities and a mean linear intercept of 80<span class="elsevierStyleHsp" style=""></span>&#956;m&#46; The same group repeated the same procedure in order to test the effects of Hepatocyte Growth Factor in injured lungs by elastase&#44; but the mean linear intercept was slightly smaller&#58; 65<span class="elsevierStyleHsp" style=""></span>&#956;m&#46;<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">30</span></a> Another study&#44;<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">31</span></a> using both C57BL&#47;6 mice and SP-C&#47;TNF-a transgenic mice&#44; using similar dosage of elastase found a mean linear intercept ranging between 60 and 85<span class="elsevierStyleHsp" style=""></span>&#956;m&#46; Comparing these morphometric data suggests that intratracheal administration of elastase seems more efficient to induce emphysema&#44; coupled with a relatively low mortality&#46; The experimental procedures used in our study to induce emphysema can be considered tolerable since the mortality index in all experimental groups remained low &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; Ishizawa et al&#46;<a class="elsevierStyleCrossRefs" href="#bib0145"><span class="elsevierStyleSup">29&#44;30</span></a> did not mention the mortality rate observed in their studies&#46;</p><p id="par0130" class="elsevierStylePara elsevierViewall">Recently&#44;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> emphysematous disturbances were induced in a transgenic strain of mice &#40;NMRI&#41;&#44; which possesses a higher endogenous anti-protease activity&#46; Two experimental groups were used to administer porcine pancreatic elastase by intratracheal instillation&#58; 5<span class="elsevierStyleHsp" style=""></span>U&#47;100<span class="elsevierStyleHsp" style=""></span>g body weight&#44; single administration and 3&#46;3<span class="elsevierStyleHsp" style=""></span>U&#47;100<span class="elsevierStyleHsp" style=""></span>g body weight&#44; repetitive administration&#46; In both treatments the absolute mortality of animals was much higher than that in our work &#40;six and five mice&#44; respectively&#41;&#46; However&#44; the authors do not mention the total number of animals used per group&#44; but it seems clear that the higher concentrations of elastase used in their study resulted in a greater mortality rate among experimental animals when compared to the present study&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> These results indicate that the experimental protocol used in our study is more applicable to induce emphysema in mice&#46;</p><p id="par0135" class="elsevierStylePara elsevierViewall">Comparing the morphological parameters of our study with the ones measured by L&#252;thje et al&#46;&#44;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> we observed that the <span class="elsevierStyleItalic">L</span>m was greater in the latter study &#40;260&#46;7<span class="elsevierStyleHsp" style=""></span>&#956;m&#41; when compared to our data &#40;99&#46;6<span class="elsevierStyleHsp" style=""></span>&#956;m&#41;&#46; The greater destruction of lung parenchyma induced in the mice by L&#252;thje et al&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> can be attributed to the greater concentration of elastase used&#44; as well as to the mouse strain used&#44; which is more susceptible to the development of pulmonary structure abnormalities&#46;</p><p id="par0140" class="elsevierStylePara elsevierViewall">The physical activity is significantly impaired in many patients with COPD&#44; significantly altering their quality of life&#46; The pathogenesis of physical incapacity is complex and involves loss of respiratory muscle strength&#44; changes in gas exchange&#44; and abnormalities in lung mechanics&#44; which reflects in exercise intolerance that is a direct consequence of impairment in respiratory mechanics&#46; However&#44; the measurement of parameters related to respiratory mechanics involved invasive or noninvasive methodology&#44; which it is not totally secure&#44; requiring a large number of animals to be used or a loss of accuracy of measurements of respiratory parameters&#44; since it does not reflect the physiological reality of an undisturbed animal&#46; The invasive measurements require that animals are anesthetized and ventilated&#44;<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">23</span></a> orotracheally or endotracheally intubated&#44; tracheotomized<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">32</span></a> or under spontaneous breathing&#46; The advantages of these methods include no stress for the animal and the evaluation of gold standard parameters&#44; such as resistance and compliance&#46; However&#44; the anesthesia could alter some physiological parameters and depending on methodology the measurement cannot be repeated&#46;<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">33</span></a> Hantos et al&#46;<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">34</span></a> tried to establish a link between the mechanical properties of the respiratory system and absolute lung volumes in elastase-induced emphysema in order to investigate how these physiological findings are related to the pathophysiological changes in lung volumes and the associated alterations in respiratory resistance and elastance in patients&#46; Thus&#44; the tissue damage did not correlate with airway dysfunction in this mouse model of emphysema&#46;<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">34</span></a></p><p id="par0145" class="elsevierStylePara elsevierViewall">On the other side&#44; noninvasive techniques are easy to perform&#44; allow spontaneously breathing&#44; and can be done in large numbers of conscious animal during the same period&#46; As previously mentioned&#44; there are two types of plethysmograph&#58; double-chamber and single-chamber whole-body instruments&#46; In the first one the animal is restrained at the neck&#46; The animals are placed in the body plethysmograph&#44; while the head protruded through a neck collar into a ventilated head exposure chamber&#46;<a class="elsevierStyleCrossRefs" href="#bib0175"><span class="elsevierStyleSup">35&#44;36</span></a> This apparatus which is attached to an amplifier is connected to a pneumotachograph and a differential pressure transducer&#44; which allows airflow measurement&#44; tidal volume&#44; minute volume and respiratory rate&#46;<a class="elsevierStyleCrossRef" href="#bib0185"><span class="elsevierStyleSup">37</span></a> These noninvasive serial measurements reduced the number of animal per study&#44; but long-term examination is not allowed due to the stress imposed by the neck collar restrained&#46; There is also a unrestrained double-chamber instrument where the animal&#39;s head and body are separated in a chamber by a rubber collar around the neck&#44; which creates two environments&#58; nasal chamber and thoracic chamber&#46; In single-chamber whole-body plethysmograph&#44; no restraint is required and the animals have free access to water and food&#46; These recordings may be done all day long but physiological measurement is indirect since it is done based on pressure changes in single-chamber whole-body plethysmograph&#46;<a class="elsevierStyleCrossRef" href="#bib0190"><span class="elsevierStyleSup">38</span></a> The results are reproducible despite of the chamber plethysmograph employed&#46; The single-chamber is appropriate to measure airway resistance reactivity to drug administration&#44; but not to evaluate tidal volume&#46; On other side&#44; the double-chamber is to provide accurate results about tidal volume and respiratory rate&#46;<a class="elsevierStyleCrossRef" href="#bib0185"><span class="elsevierStyleSup">37</span></a> When this technique is performed in a double chamber with restrained animal records of inspiratory and expiratory flows&#44; tidal volume&#44; minute volume and respiratory rate can be done directly&#44; but it is not possible to measure transpulmonary pressure or airway resistance&#46; The noninvasive measurements with unrestrained animal in a single chamber allow physiological measurement based on pressure alterations inside the chamber&#44; thus the inspiratory and expiratory flows are evaluated indirectly&#44; and the transpulmonary pressure cannot be analyzed&#46; The limitation is the shortage of accuracy on the evaluation of physiological parameters&#44; such as respiratory rate and tidal volume&#46;<a class="elsevierStyleCrossRef" href="#bib0195"><span class="elsevierStyleSup">39</span></a></p><p id="par0150" class="elsevierStylePara elsevierViewall">In addition to the reasons described above&#44; that justify the use of treadmill exercise to assess the functional limitations of the respiratory system induced by emphysema&#44;<a class="elsevierStyleCrossRefs" href="#bib0145"><span class="elsevierStyleSup">29&#8211;31</span></a> there is the fact that acute physical exercise is known to markedly increase the blood flow and oxygen uptake in the lung&#46; The severity of pulmonary parenchyma lesions on exercise performance can be easily evaluated by ergometric tests&#44; and in experimental models of emphysema this fact may be evaluated by testing exercise capacity in treadmill&#44; as performed by L&#252;thje et al&#46;&#44;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> which demonstrated a reduction in relative run distance in emphysematous mice&#46; Here&#44; not only the distance run and exercise time were measured but the consumption of oxygen and carbon dioxide were evaluated&#46; The results obtained demonstrated that emphysema induction committed the exercise capacity probably due to an impairment in gas exchange &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a> and <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46;</p><p id="par0155" class="elsevierStylePara elsevierViewall">In our work&#44; with a single intratracheal administration of elastase and a small degree of inclination in the treadmill&#44; we observed a reduction in running distance of 30&#46;5&#37; when compared to pre-treatment exercise capacity of emphysematous mice&#46; The greatest decrease in maximum velocity was also seen in the group treated with elastase by intratracheal route &#40;<a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46; A reduction in maximum velocity during treadmill exercise was also observed in a rat model of emphysema after intratracheal administration of elastase&#46;<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">34</span></a> The treadmill performance of mice in the study by L&#252;thje and co-workers revealed that there was no difference in running distance between control and single intratracheal administration of elastase groups&#44; whereas the reduction in running distance in the group with repetitive intratracheal administration of elastase was 29&#46;7&#37;&#46; Therefore&#44; although the pulmonary tissue destruction was more severe in the study by L&#252;thje and co-workers&#44; as shown by liner intercept length&#44; probably due to strain susceptibility and the number of elastase administration&#44; our experimental procedure resulted in the same level of serious functional impairment&#44; as revealed by the exercise performance&#46; Different from the previous works&#44; we analysed the dynamics of gas exchange during exercise&#46; The augment in oxygen consumption &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>A&#41; could reflect the ability of the heart to increase cardiac output in order to attend the metabolic demands of animal during physical effort&#44; trying to hold the blood oxygen partial pressure in the face of blood carbon dioxide partial pressure altered&#46; The maintenance of carbon dioxide production &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>B&#41; could mean the impairment of gas exchange&#46; These respiratory dysfunction is reflected in a lower exercise capacity and could be consequence of pulmonary parenchyma lesion&#46;</p><p id="par0160" class="elsevierStylePara elsevierViewall">Up to date&#44; all of the studies inducing emphysema in animal models are insufficiently capable of reproducing the slow and progressive inflammatory process that leads to chronic pulmonary injury in humans&#46; However&#44; they helped to elucidate the pathogenic mechanisms and the development of new clinical therapeutic protocols&#46; Different animals such as hamsters&#44; rats&#44; and mice were used to establish models of emphysema&#44; which are based on genetic manipulations &#40;in mice&#41;&#44; exposure to cigarette smoke&#44; or administration of proteinases into the airway&#46; Knockout or transgenic mice models for emphysema revealed different genes that could be involved in the pathogenesis of emphysema&#44; such as platelet derived growth factor A&#44;<a class="elsevierStyleCrossRef" href="#bib0200"><span class="elsevierStyleSup">40</span></a> fibroblast growth factor receptors 3 and 4&#44;<a class="elsevierStyleCrossRef" href="#bib0205"><span class="elsevierStyleSup">41</span></a> metalloproteinase-1 transgenic mice&#44;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">15</span></a> platelet derived growth factor B transgenic mice&#44;<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">42</span></a> macrophage elastase&#44;<a class="elsevierStyleCrossRef" href="#bib0215"><span class="elsevierStyleSup">43</span></a> and neutrophil elastase&#44;<a class="elsevierStyleCrossRef" href="#bib0220"><span class="elsevierStyleSup">44</span></a> the last ones given resistance to smoke-induced emphysema&#46; However&#44; it is noteworthy that these gene-targeted mice may have alterations in lung structure&#44; such as alveolar enlargement&#44; possibly associated to alterations in lung development instead of emphysema development due to destruction in pulmonary parenchyma&#44; since these genes are expressed throughout the tissue development&#44; growth&#44; and maturation&#46;<a class="elsevierStyleCrossRef" href="#bib0225"><span class="elsevierStyleSup">45</span></a> The smoke-induced animal models of COPD include nose-only exposure or whole body exposure&#46; This model may be developed in different animal species&#44; such as rat&#44;<a class="elsevierStyleCrossRef" href="#bib0230"><span class="elsevierStyleSup">46</span></a> guinea-pig&#44;<a class="elsevierStyleCrossRef" href="#bib0235"><span class="elsevierStyleSup">47</span></a> or mouse&#46;<a class="elsevierStyleCrossRef" href="#bib0240"><span class="elsevierStyleSup">48</span></a> The guinea-pig reproduces the most appropriate model of emphysema&#44; but there are many problems related to the cost&#44; shortage of tools to investigate molecular mechanisms&#44; and reduced number of reagent specific to this animal species&#46;<a class="elsevierStyleCrossRefs" href="#bib0020"><span class="elsevierStyleSup">4&#44;49</span></a> Rats are very resistant and if the time and volume of smoke exposure are extrapolated these animals developed in specific abnormalities&#46;<a class="elsevierStyleCrossRef" href="#bib0250"><span class="elsevierStyleSup">50</span></a> With regards to mice&#44; in addition to the advantages described above&#44; functional parameters such as compliance&#44; can be accurately measured&#46;<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">4</span></a> However&#44; the smoke-induced-emphysema model is not so reproductible&#44;<a class="elsevierStyleCrossRefs" href="#bib0255"><span class="elsevierStyleSup">51&#44;52</span></a> the time for developing the disease is long &#40;about six months&#41;&#44; produce only a mild disease and after the cessation of smoke the lesions do not appear to progress&#46;<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">4</span></a> Currently&#44; mice are considered the gold standard to reproduce and study many human diseases&#44; since the mouse genome shows a great similarity to the human genome&#44; having about 300 genes uniquely present in one species or the other&#46;<a class="elsevierStyleCrossRef" href="#bib0265"><span class="elsevierStyleSup">53</span></a> Furthermore&#44; more than 10&#44;000 genetic markers have been mapped in the mouse&#44; a large number of inbred strains and transgenic mice are available&#44; and data on the animals&#8217; anatomy&#44; biology&#44; immunology&#44; and physiology are available&#46;</p><p id="par0165" class="elsevierStylePara elsevierViewall">The model tested in our study provides some advantages&#44; including the use of a lower elastase concentration when compared to other studies&#46; Nonetheless&#44; it induced severe structural abnormalities that may be responsible for functional disturbances characterizing pulmonary emphysema&#46; Furthermore&#44; the emphysema could be induced with a very low mortality rate and a high degree of reproducibility&#44; which could be functional analyzed by non-invasive technique that reflects the physical impairment associated to structural abnormalities characteristic of pulmonary emphysema&#46;</p></span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Conflicts of interest</span><p id="par0170" class="elsevierStylePara elsevierViewall">The authors have no conflicts of interest to declare&#46;</p></span></span>"
    "textoCompletoSecciones" => array:1 [
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        0 => array:2 [
          "identificador" => "xres547591"
          "titulo" => "Abstract"
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        1 => array:2 [
          "identificador" => "xpalclavsec565468"
          "titulo" => "Keywords"
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        2 => array:2 [
          "identificador" => "xres547590"
          "titulo" => "Resumo"
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          "titulo" => "Palavras-chave"
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        4 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Introduction"
        ]
        5 => array:3 [
          "identificador" => "sec0010"
          "titulo" => "Materials and methods"
          "secciones" => array:6 [
            0 => array:2 [
              "identificador" => "sec0015"
              "titulo" => "Animals"
            ]
            1 => array:2 [
              "identificador" => "sec0020"
              "titulo" => "Experimental groups"
            ]
            2 => array:2 [
              "identificador" => "sec0025"
              "titulo" => "Emphysema induction"
            ]
            3 => array:2 [
              "identificador" => "sec0030"
              "titulo" => "Treadmill"
            ]
            4 => array:2 [
              "identificador" => "sec0035"
              "titulo" => "Histopathological analysis"
            ]
            5 => array:2 [
              "identificador" => "sec0040"
              "titulo" => "Statistical analysis"
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          ]
        ]
        6 => array:3 [
          "identificador" => "sec0045"
          "titulo" => "Results"
          "secciones" => array:3 [
            0 => array:2 [
              "identificador" => "sec0050"
              "titulo" => "Safety of the procedure &#8211; mortality"
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            1 => array:2 [
              "identificador" => "sec0055"
              "titulo" => "Functional analysis &#8211; ergometry"
            ]
            2 => array:2 [
              "identificador" => "sec0060"
              "titulo" => "Morphometric analysis &#8211; mean linear interseptum measurement"
            ]
          ]
        ]
        7 => array:2 [
          "identificador" => "sec0065"
          "titulo" => "Discussion and conclusion"
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          "identificador" => "sec0070"
          "titulo" => "Conflicts of interest"
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        9 => array:2 [
          "identificador" => "xack184907"
          "titulo" => "Acknowledgements"
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        10 => array:1 [
          "titulo" => "References"
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      ]
    ]
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    "fechaRecibido" => "2011-07-06"
    "fechaAceptado" => "2011-12-23"
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        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec565468"
          "palabras" => array:4 [
            0 => "Pulmonary emphysema"
            1 => "Ergometric test"
            2 => "Porcine elastase"
            3 => "C57Bl&#47;6"
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        ]
      ]
      "pt" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palavras-chave"
          "identificador" => "xpalclavsec565469"
          "palabras" => array:4 [
            0 => "Enfisema pulmonar"
            1 => "ensaio ergom&#233;trico"
            2 => "Elastase su&#237;na"
            3 => "C57Bl&#47;6"
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        ]
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    "resumen" => array:2 [
      "en" => array:2 [
        "titulo" => "Abstract"
        "resumen" => "<span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Several experimental studies of pulmonary emphysema using animal models have been described in the literature&#46; However&#44; only a few of these studies have focused on the assessment of ergometric function as a non-invasive technique to validate the methodology used for induction of experimental emphysema&#46; Additionally&#44; functional assessments of emphysema are rarely correlated with morphological pulmonary abnormalities caused by induced emphysema&#46; The present study aimed to evaluate the effects of elastase administered by tracheal puncture on pulmonary parenchyma and their corresponding functional impairment&#46; This was evaluated by measuring exercise capacity in C57Bl&#47;6 mice in order to establish a reproducible and safe methodology of inducing experimental emphysema&#46; Thirty six mice underwent ergometric tests before and 28 days after elastase administration&#46; Pancreatic porcine elastase solution was administered by tracheal puncture&#44; which resulted in a significantly decreased exercise capacity&#44; shown by a shorter distance run &#40;&#8722;30&#46;5&#37;&#41; and a lower mean velocity &#40;&#8722;15&#37;&#41;&#44; as well as in failure to increase the elimination of carbon dioxide&#46; The mean linear intercept increased significantly by 50&#37; in tracheal elastase administration&#46; In conclusion&#44; application of elastase by tracheal function in C57Bl&#47;6 induces emphysema&#44; as validated by morphometric analyses&#44; and resulted in a significantly lower exercise capacity&#44; while resulting in a low mortality rate&#46;</p></span>"
      ]
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        "titulo" => "Resumo"
        "resumen" => "<span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">V&#225;rios estudos experimentais de enfisema pulmonar em modelos animais t&#234;m sido descritos na literatura cient&#237;fica&#46; No entanto&#44; apenas alguns destes estudos t&#234;m sido concentrados na avalia&#231;&#227;o da fun&#231;&#227;o ergom&#233;trica como t&#233;cnica n&#227;o-invasiva para validar a metodologia utilizada para a indu&#231;&#227;o do enfisema experimental&#46; Al&#233;m disso&#44; as avalia&#231;&#245;es funcionais de enfisema raramente se encontram correlacionadas com anomalias morfol&#243;gicas pulmonares causadas por enfisema induzido&#46; O presente estudo teve como objetivo avaliar os efeitos da elastase administrada por pun&#231;&#227;o traqueal no par&#234;nquima pulmonar e a sua disfun&#231;&#227;o funcional correspondente&#46; Esta foi avaliada atrav&#233;s da medi&#231;&#227;o da capacidade de exerc&#237;cio em ratos C57Bl&#47;6&#44; de forma a estabelecer uma metodologia reprodut&#237;vel e segura de induzir o enfisema experimental&#46; Trinta e seis ratos foram submetidos a testes ergom&#233;tricos antes e 28 dias ap&#243;s a administra&#231;&#227;o de elastase&#46; A solu&#231;&#227;o de elastase pancre&#225;tica su&#237;na foi administrada por pun&#231;&#227;o traqueal&#44; o que resultou numa diminui&#231;&#227;o significativamente da capacidade de exerc&#237;cio&#44; demonstrada pela diminui&#231;&#227;o da dist&#226;ncia percorrida &#40;menos de 30&#44;5&#37;&#41; e por uma velocidade m&#233;dia inferior &#40;menos de 15&#37;&#41;&#44; assim como pela incapacidade de aumentar a elimina&#231;&#227;o de di&#243;xido de carbono&#46; A intersec&#231;&#227;o linear m&#233;dia aumentou significativamente em 50&#37; na administra&#231;&#227;o traqueal da elastase&#46; Em conclus&#227;o&#44; a aplica&#231;&#227;o de elastase por pun&#231;&#227;o traqueal em ratos C57Bl&#47;6 induz enfisema&#44; conforme foi validado por an&#225;lises morfom&#233;tricas&#44; e resultou numa capacidade de exerc&#237;cio significativamente mais baixa&#44; embora se tenha obtido uma baixa taxa de mortalidade&#46;</p></span>"
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          "en" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Mice performance during exercise on a motorized treadmill evaluated by maximum velocity reached and maximum distance covered before &#40;pre&#41; and after treatment &#40;post&#41; and the respective percentage change &#40;&#37;&#41;&#46; Groups as in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#46;</p>"
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Original article
Alterations in pulmonary structure by elastase administration in a model of emphysema in mice is associated with functional disturbances
Alterações na estrutura pulmonar devido à administração de elastase num modelo de enfisema em ratos encontram-se associadas a distúrbios funcionais
D. Vidala,b, G. Fortunatoa,c, W. Kleind,e,f, L. Cortizoa, J. Vasconcelosa,b, R. Ribeiro-dos-Santosa,b, M. Soaresa,b, S. Macambiraa,b,g,
Corresponding author
simone@bahia.fiocruz.br

Corresponding author.
a Laboratório de Engenharia tecidual e Imunofarmacologia, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, BA, Brazil
b Centro de Biotecnologia e Terapia Celular, Hospital São Rafael, Salvador, BA, Brazil
c Programa de Pós-Graduação em Biotecnologia, Universidade Estadual de Feira de Santana, Feira de Santana, BA, Brazil
d Faculdade de Biologia, Instituto de Biologia, Universidade Federal da Bahia, Salvador, BA, Brazil
e Instituto de Biociências, Instituto Nacional de Ciência e Tecnologia em Fisiologia Comparada, UNESP, Rio Claro, SP, Brazil
f Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, SP, Brazil
g Laboratório de Eletrofisiologia Cardíaca “Prof Antônio Paes de Carvalho”, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, RJ, Brazil
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    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Emphysema is a chronic obstructive pulmonary disease &#40;COPD&#41;&#44; limiting air flow during expiration&#44; and is associated with an abnormal inflammatory response of the lungs to noxious particles or gases &#40;mainly cigarette smoke&#41;&#46; This disease is characterized by a permanent abnormal dilatation of alveolar spaces&#46; The destruction of pulmonary parenchyma impairs alveolar gas exchange&#44; compromising the physical capacity of a patient since it is associated with airflow limitations that are not fully reversible&#46;</p><p id="par0010" class="elsevierStylePara elsevierViewall">No therapy capable of reversing emphysematous tissue lesions is available to date and&#44; in severe chronic stages the only treatment that remains is lung transplantation&#44; representing a procedure with high levels of morbidity and mortality&#46; Emphysema&#44; together with other types of COPD&#44; are responsible for more than 2&#46;5 million deaths every year&#44; representing the fifth leading cause of mortality in the world&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">The severity of its pathology together with the lack of any effective treatment transforms emphysema into a great medical challenge&#46; There is a need for studies aiming to understand the pathogenic cellular mechanisms causing tissue destruction in order to elucidate the disease and to open new therapeutic avenues&#46; Another aggravator is the fact that a considerable variation exists in the course of the disease among different smokers&#46; Only about 15&#37; of smokers develop COPD&#46;<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1&#44;2</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">Animal models represent a fundamental instrument to correlate pre-clinical research with clinical studies&#46; The experimental model allows the detailed investigation of different factors influencing emphysema&#46; These factors include inflammatory cell recruitment&#44; genetic background&#44; abnormal matrix repair&#44; lung cell apoptosis&#44; and misbalance between apoptosis and replenishment of structural cells in the lung&#46; Additional factors are research of potential therapeutic agents and strategies&#44; such as administration of stem cells or different growth factors&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a></p><p id="par0025" class="elsevierStylePara elsevierViewall">Until now there are many experimental models of COPD&#44; each of them with advantages and disadvantages&#46; In reality&#44; a number of approaches have been tried because until now none of them constitutes a model that exactly reproduces all phases of development and clinical features of emphysema or any other abnormalities that make up this clinical entity&#44; the COPD&#46; Other factor that affects the reproducibility of data is the differences in anatomical structure with respect to development&#44; maturation&#44; structural organization of respiratory branches&#44; and constituent cells and vascularization&#46;<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">4</span></a> On the other side&#44; some common characteristics can be observed between animal model and human disease&#46; For example&#44; rodents develop the phenomenon of metaplasia induced by reaction to injury&#44;<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> which is a frequently observed response to an insult in different organs in humans&#44; such as in respiratory tract&#44; urinary bladder&#44; and esophagus&#46;</p><p id="par0030" class="elsevierStylePara elsevierViewall">The first animal model of emphysema was developed more than 40 years ago<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a> and it was evoked in rats by intratracheal instillation of the plant proteinase papain&#46; The experimental models of emphysema are continuously improving and became a research tool for several groups of scientists&#46; These include enzyme airway administration model&#44;<a class="elsevierStyleCrossRefs" href="#bib0035"><span class="elsevierStyleSup">7&#44;8</span></a> tobacco smoking model&#44;<a class="elsevierStyleCrossRefs" href="#bib0045"><span class="elsevierStyleSup">9&#44;10</span></a> apoptosis induced emphysema by intratracheal instillation of activated caspase-3&#44;<a class="elsevierStyleCrossRef" href="#bib0055"><span class="elsevierStyleSup">11</span></a> AIAT deficient animals&#44;<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">12</span></a> natural mutants&#44;<a class="elsevierStyleCrossRef" href="#bib0065"><span class="elsevierStyleSup">13</span></a> knockout mice&#44;<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">14</span></a> and transgenic mice&#46;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">15</span></a> Despite the model chosen all of them present restrictions&#46;<a class="elsevierStyleCrossRefs" href="#bib0080"><span class="elsevierStyleSup">16&#8211;22</span></a></p><p id="par0035" class="elsevierStylePara elsevierViewall">Taking these informations into account&#44; in the present study we chose to reproduce human pulmonary emphysema in mouse model using intratracheal instillation of porcine pancreatic elastase because it is used for more than three decades and has been well characterized&#46;<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">29</span></a> Until now it is considered to be the most consistent and impressive model of airspace enlargement&#46;<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">30</span></a> This is due to the exposure of elastin fibers to porcine pancreatic elastase leading to acute lung inflammation and pulmonary parenchyma destruction&#46; This model is very appropriate to investigate the relation between structure and function abnormalities characteristics of emphysema progression&#44; as well as elucidated factors associated to these disturbances and collagen remodeling failure related to this pathology&#46;<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">31</span></a> This model was used to investigate and validate a new methodology to evaluate the dynamic lung mechanical function in experimental model of emphysema&#46;<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">32</span></a> The pathogenesis for emphysema includes many events such as inflammation&#44; elastase&#44; matrix metalloprotease imbalance&#44; apoptosis&#44; and oxidative stress&#44; which are reproduced by elastase administration in animal models&#46;<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">33</span></a> We chose to develop the model in female C57Bl&#47;6 mice based on clinical evidences that point out women are more susceptible to emphysema development than men<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">34</span></a> and on pre-clinical assays that demonstrated the higher susceptibility of female A&#47;J mice to emphysema&#46;<a class="elsevierStyleCrossRef" href="#bib0175"><span class="elsevierStyleSup">35</span></a> The use of an appropriate model of emphysema is essential to investigate relevant questions involved in the pathophysiology of the disease and to test the therapeutic potential of new drugs&#46; Another essential point is the use of methods to evaluate the physiological parameters of respiratory in the emphysema model&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">Evaluation of pulmonary function and measurement of physiological parameters are crucial to studies that investigate new therapeutic strategies&#44; pathogenesis of disease and toxicology&#46; There are invasive and noninvasive pulmonary function tests available which are sensitive in detecting abnormalities in mechanical respiratory&#46; The invasive methodology evolution allowed the evaluation of relevant parameters transpulmonary pressure&#44; expiratory and inspiratory flows&#44; such as pulmonary resistance and compliance&#46; Although these procedures give precise data&#44; they involve invasive surgical procedures<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">23</span></a> &#40;orotracheal intubated animal&#41; and anesthesia that impose non-physiological conditions to experiment that may generate significant artifacts&#44; such as decreased respiratory frequency&#44;<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">24</span></a> besides the risk of death of animals due to the procedure which implies the use of a larger number of animals per experimental group&#46; The non-invasive methodology started in the end of 70s<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">25</span></a> and there are basically two types of plethysmograph&#58; double-chamber and single-chamber whole-body instrument&#46; This noninvasive serial measurements reduced the number of animal per study&#44; but long-term examination is not allowed due to the stress imposed by of the neck collar restrained&#46; Noninvasive techniques to evaluate respiratory parameters in conscious mice are easy&#44; convenient&#44; repetitive and reproducible&#44; appropriate for quick and repeatable screening of respiratory function in a large number of conscious animals&#44;<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">26</span></a> however are not so accurate as the invasive method&#44; and some important data about pulmonary function may be misleading&#46;</p><p id="par0045" class="elsevierStylePara elsevierViewall">This kind of evaluation is essential to reveal information about pulmonary function but does not provide data on the degree of impairment of physical capacity of the animal&#46; L&#252;thje et al&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> demonstrated exercise intolerance by treadmill as a consequence of pulmonary emphysema and its systemic consequence in elastase-induced-emphysema model in transgenic mice&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">Following this rational&#44; we sought to evaluate a different procedure to establish experimental pulmonary emphysema in C57Bl&#47;6 using elastase via tracheal puncture&#46; To prove the efficacy of emphysema induction&#44; we investigated the degree of impairment of physical capacity by the evaluation of respiratory function during exercise testing on treadmill&#44; acquiring data about distance run&#44; exercise time&#44; oxygen consumption&#44; and carbon dioxide production&#46; To evaluate the structure alterations we measured LM&#44; an objective parameter that represents the average distance between alveolar walls&#46; The increase of <span class="elsevierStyleItalic">L</span>m with age is relatively small and it is independent of body size&#46; This measurement is easy to make and is independent of the observer&#46; This measurement is considered to be suitable for detailed studies that aim to correlate structure and function disturbances in lungs&#46;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">28</span></a></p><p id="par0055" class="elsevierStylePara elsevierViewall">By the investigation of functional disturbances and structural alterations associated with elastase-induced emphysema in mice&#44; we aimed to establish an experimental model with high reproducibility and low mortality&#46; We analyzed safety&#44; feasibility and reproducibility of our model applying intratracheally an intermediary dose of elastase to produce the enlargement of alveolar space and its consequences on respiratory function&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Materials and methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Animals</span><p id="par0060" class="elsevierStylePara elsevierViewall">Two-month old female C57Bl&#47;6 mice &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>36&#41;&#44; weighing around 20<span class="elsevierStyleHsp" style=""></span>g&#44; raised and maintained at the animal facilities at the Gon&#231;alo Moniz Research Center&#44; FIOCRUZ&#44; were used in the experiments&#44; and were provided with rodent diet and water <span class="elsevierStyleItalic">ad libitum</span>&#46; All animals were euthanized in a CO<span class="elsevierStyleInf">2</span> chamber&#44; and handled according the NIH guidelines for ethical use of laboratory animals&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Experimental groups</span><p id="par0065" class="elsevierStylePara elsevierViewall">The animals were randomly divided into three groups&#58; &#40;A&#41; control&#58; non-manipulated animals &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>12&#41;&#59; &#40;B&#41; intratracheal elastase&#58; 100<span class="elsevierStyleHsp" style=""></span>&#956;l &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>12&#41;&#59; and &#40;C&#41; intratracheal PBS 100<span class="elsevierStyleHsp" style=""></span>&#956;l &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>12&#41;&#46;</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Emphysema induction</span><p id="par0070" class="elsevierStylePara elsevierViewall">For elastase instillation&#44; mice were anaesthetized with ketamine &#40;Vetbrands&#41; and atropine &#40;Allergan&#41;&#44; intubated orotracheally&#44; and ventilated using a ventilator&#47;respirator &#40;Mini-Vent Small Animal Ventilators&#44; Kent Scientific&#41;&#46; Experimental animals received 2<span class="elsevierStyleHsp" style=""></span>U&#47;100<span class="elsevierStyleHsp" style=""></span>g body weight porcine pancreatic elastase &#40;Sigma&#44; Aldrich&#44; Taufkirchen&#44; Germany&#41; dissolved in 100<span class="elsevierStyleHsp" style=""></span>&#956;l phosphate-buffered saline &#40;PBS&#41;solution or 100<span class="elsevierStyleHsp" style=""></span>&#956;l PBS alone&#46; Control animals were not manipulated&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">The respective solution was administered intratracheally via tubing followed by 200<span class="elsevierStyleHsp" style=""></span>&#956;l of air for an even distribution of the liquid throughout both lungs&#46; The animals were submitted to anterior cervical incision in order to visualize the trachea and make the tracheal puncture to administer one of the solutions&#46; Afterwards&#44; mice were sutured&#44; kept on a warm plate &#40;30<span class="elsevierStyleHsp" style=""></span>&#176;C&#41; until restoration of spontaneous breathing and then were extubated&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Treadmill</span><p id="par0080" class="elsevierStylePara elsevierViewall">A motor-driven treadmill chamber for one animal &#40;LE 8700&#44; Panlab&#44; Barcelona&#44; Spain&#41; was used to exercise the animals&#46; The speed of the treadmill and the intensity in milliamps of the electric shock applied to an stainless steel grid at the rear end of the treadmill were controlled by a potentiometer &#40;LE 8700 treadmill control&#44; Panlab&#41;&#46; Room air was pumped into the chamber at a controlled flow rate &#40;700<span class="elsevierStyleHsp" style=""></span>ml&#47;min&#41; by a chamber air supplier &#40;OXYLET LE 400&#44; Panlab&#41;&#46; Outflow was directed to an oxygen and carbon dioxide analyzer &#40;Oxylet 00&#59; Panlab&#41; to measure consumption of oxygen and production of carbon dioxide&#46; The mean room temperature was maintained at 21<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1<span class="elsevierStyleHsp" style=""></span>&#176;C&#46; After an adaptation period of 40<span class="elsevierStyleHsp" style=""></span>min in the treadmill chamber the mice were exercised at different velocities&#44; starting at 7&#46;2<span class="elsevierStyleHsp" style=""></span>m&#47;min and increasing the velocity 7&#46;2<span class="elsevierStyleHsp" style=""></span>m&#47;min every 10<span class="elsevierStyleHsp" style=""></span>min&#46; The inclination of the treadmill was maintained at an uphill angle of 10&#176;&#46; Velocities were increased until the animal could no longer sustain a given speed and remained for more than ten seconds on the electrified grid&#44; which provided an electrical stimulus &#40;1 milliamp&#41; to keep the mice running&#46; Total running distance and running time were recorded&#46; Treadmill tests were carried out on all mice before the induction of emphysema and 28 days after experimental procedures&#46;</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Histopathological analysis</span><p id="par0085" class="elsevierStylePara elsevierViewall">Twenty-eight days after application of elastase or PBS&#44; the mice were euthanized using CO<span class="elsevierStyleInf">2</span> and the lungs were exposed&#46; The trachea was canullated with gelco number 18 and the lungs were perfused with buffered 4&#37; formalin applying a constant transpulmonary pressure of 20<span class="elsevierStyleHsp" style=""></span>cm H<span class="elsevierStyleInf">2</span>O for 2<span class="elsevierStyleHsp" style=""></span>h&#46; After this procedure the trachea was sutured in order to hold the intrapulmonary pressure at 20<span class="elsevierStyleHsp" style=""></span>cm H<span class="elsevierStyleInf">2</span>O and the entire cardiopulmonary tissue block was removed and fixed in formalin &#40;4&#37;&#41;&#46;</p><p id="par0090" class="elsevierStylePara elsevierViewall">Morphologic examinations were performed following Thurlbeck<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">28</span></a> method modified&#46; Briefly&#44; the mean linear intercept &#40;<span class="elsevierStyleItalic">L</span>m&#41; of each lung was determined using light microscopy on 20 randomly selected fields&#44; originating from randomly selected tissue samples covering the entire lung and containing apical as well as basal areas of the organ&#46; Five pictures were taken of each lung&#46; The <span class="elsevierStyleItalic">L</span>m as indicator of air space size was calculated from counting lines of defined length that were randomly placed on each of the 20 lung sections of 5<span class="elsevierStyleHsp" style=""></span>&#956;m and the number of intercepts crossing the lines counted&#46; The <span class="elsevierStyleItalic">L</span>m was calculated from the length of the lines multiplied by the number of the lines divided by the sum of all counted intercepts&#46;</p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Statistical analysis</span><p id="par0095" class="elsevierStylePara elsevierViewall">Numeric values were expressed as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SD unless stated otherwise&#46; Treadmill performance was analysed using a paired <span class="elsevierStyleItalic">t</span>-test to compare groups before and after treatment and morphometric data were compared applying a One-way ANOVA followed by a Tukey test to identify groups that are significantly different&#46; A value of <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05 was considered statistically significant&#46;</p></span></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Results</span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Safety of the procedure &#8211; mortality</span><p id="par0100" class="elsevierStylePara elsevierViewall">Four out of 36 mice &#40;11&#46;1&#37;&#41; died during the experimental procedure&#46; The groups manipulated by intratracheal function showed a similar mortality &#40;two death per group&#41;&#44; independent of the kind of solution administrated &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Functional analysis &#8211; ergometry</span><p id="par0105" class="elsevierStylePara elsevierViewall">Maximum velocity and running distance reached by each group before &#40;pre&#41; and after &#40;post&#41; administration of PBS or elastase are summarized in <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#46;</p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><p id="par0110" class="elsevierStylePara elsevierViewall">Before the manipulation&#44; all mice were capable of exercising on the treadmill&#46; After manipulation&#44; intratracheal elastase administration &#40;group B&#41; showed a significant decrease in the maximum exercise velocity being able to sustain treadmill velocities up to 15&#46;1<span class="elsevierStyleHsp" style=""></span>m&#47;min &#40;<a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46; These differences resulted in a significant reduction in distance covered by tracheal elastase-treated mice when compared to tracheal saline-treated mice&#44; reducing running distance by 30&#46;5&#37; in the former group &#40;<a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46;</p><p id="par0115" class="elsevierStylePara elsevierViewall">Concerning oxygen consumption and carbon dioxide production&#44; all mice treated with elastase increased oxygen consumption after induction when compared at the same speed before and after administration of elastase &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>A&#41;&#46; In relation to the production of carbon dioxide&#44; no significant difference was observed at the same speed before and after administration of elastase &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>B&#41;&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Morphometric analysis &#8211; mean linear interseptum measurement</span><p id="par0120" class="elsevierStylePara elsevierViewall">The administration of 100<span class="elsevierStyleHsp" style=""></span>&#956;l of elastase by the tracheal route significantly increased <span class="elsevierStyleItalic">L</span>m &#40;99&#46;6<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2&#46;9<span class="elsevierStyleHsp" style=""></span>&#956;m&#41; when compared to intratracheal saline-treated &#40;33&#46;3<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;6<span class="elsevierStyleHsp" style=""></span>&#956;m&#41; and non-manipulated mice &#40;25&#46;6<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;1<span class="elsevierStyleHsp" style=""></span>&#956;m&#41;&#46; A slight&#44; but significant&#44; increase in <span class="elsevierStyleItalic">L</span>m was found comparing traqueal application of saline with non-manipulated animals &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41;&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia></span></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Discussion and conclusion</span><p id="par0125" class="elsevierStylePara elsevierViewall">In this work we induced emphysema by intratracheal instillation of porcine pancreatic elastase&#44; which resulted in a mean linear intercept of 99&#46;6<span class="elsevierStyleHsp" style=""></span>&#956;m&#46; Ishizawa et al&#46;<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">29</span></a> applied the same concentration of elastase by intranasal instillation to induce the experimental emphysema in mice and&#44; after three weeks&#44; they observed pulmonary abnormalities and a mean linear intercept of 80<span class="elsevierStyleHsp" style=""></span>&#956;m&#46; The same group repeated the same procedure in order to test the effects of Hepatocyte Growth Factor in injured lungs by elastase&#44; but the mean linear intercept was slightly smaller&#58; 65<span class="elsevierStyleHsp" style=""></span>&#956;m&#46;<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">30</span></a> Another study&#44;<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">31</span></a> using both C57BL&#47;6 mice and SP-C&#47;TNF-a transgenic mice&#44; using similar dosage of elastase found a mean linear intercept ranging between 60 and 85<span class="elsevierStyleHsp" style=""></span>&#956;m&#46; Comparing these morphometric data suggests that intratracheal administration of elastase seems more efficient to induce emphysema&#44; coupled with a relatively low mortality&#46; The experimental procedures used in our study to induce emphysema can be considered tolerable since the mortality index in all experimental groups remained low &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; Ishizawa et al&#46;<a class="elsevierStyleCrossRefs" href="#bib0145"><span class="elsevierStyleSup">29&#44;30</span></a> did not mention the mortality rate observed in their studies&#46;</p><p id="par0130" class="elsevierStylePara elsevierViewall">Recently&#44;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> emphysematous disturbances were induced in a transgenic strain of mice &#40;NMRI&#41;&#44; which possesses a higher endogenous anti-protease activity&#46; Two experimental groups were used to administer porcine pancreatic elastase by intratracheal instillation&#58; 5<span class="elsevierStyleHsp" style=""></span>U&#47;100<span class="elsevierStyleHsp" style=""></span>g body weight&#44; single administration and 3&#46;3<span class="elsevierStyleHsp" style=""></span>U&#47;100<span class="elsevierStyleHsp" style=""></span>g body weight&#44; repetitive administration&#46; In both treatments the absolute mortality of animals was much higher than that in our work &#40;six and five mice&#44; respectively&#41;&#46; However&#44; the authors do not mention the total number of animals used per group&#44; but it seems clear that the higher concentrations of elastase used in their study resulted in a greater mortality rate among experimental animals when compared to the present study&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> These results indicate that the experimental protocol used in our study is more applicable to induce emphysema in mice&#46;</p><p id="par0135" class="elsevierStylePara elsevierViewall">Comparing the morphological parameters of our study with the ones measured by L&#252;thje et al&#46;&#44;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> we observed that the <span class="elsevierStyleItalic">L</span>m was greater in the latter study &#40;260&#46;7<span class="elsevierStyleHsp" style=""></span>&#956;m&#41; when compared to our data &#40;99&#46;6<span class="elsevierStyleHsp" style=""></span>&#956;m&#41;&#46; The greater destruction of lung parenchyma induced in the mice by L&#252;thje et al&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> can be attributed to the greater concentration of elastase used&#44; as well as to the mouse strain used&#44; which is more susceptible to the development of pulmonary structure abnormalities&#46;</p><p id="par0140" class="elsevierStylePara elsevierViewall">The physical activity is significantly impaired in many patients with COPD&#44; significantly altering their quality of life&#46; The pathogenesis of physical incapacity is complex and involves loss of respiratory muscle strength&#44; changes in gas exchange&#44; and abnormalities in lung mechanics&#44; which reflects in exercise intolerance that is a direct consequence of impairment in respiratory mechanics&#46; However&#44; the measurement of parameters related to respiratory mechanics involved invasive or noninvasive methodology&#44; which it is not totally secure&#44; requiring a large number of animals to be used or a loss of accuracy of measurements of respiratory parameters&#44; since it does not reflect the physiological reality of an undisturbed animal&#46; The invasive measurements require that animals are anesthetized and ventilated&#44;<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">23</span></a> orotracheally or endotracheally intubated&#44; tracheotomized<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">32</span></a> or under spontaneous breathing&#46; The advantages of these methods include no stress for the animal and the evaluation of gold standard parameters&#44; such as resistance and compliance&#46; However&#44; the anesthesia could alter some physiological parameters and depending on methodology the measurement cannot be repeated&#46;<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">33</span></a> Hantos et al&#46;<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">34</span></a> tried to establish a link between the mechanical properties of the respiratory system and absolute lung volumes in elastase-induced emphysema in order to investigate how these physiological findings are related to the pathophysiological changes in lung volumes and the associated alterations in respiratory resistance and elastance in patients&#46; Thus&#44; the tissue damage did not correlate with airway dysfunction in this mouse model of emphysema&#46;<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">34</span></a></p><p id="par0145" class="elsevierStylePara elsevierViewall">On the other side&#44; noninvasive techniques are easy to perform&#44; allow spontaneously breathing&#44; and can be done in large numbers of conscious animal during the same period&#46; As previously mentioned&#44; there are two types of plethysmograph&#58; double-chamber and single-chamber whole-body instruments&#46; In the first one the animal is restrained at the neck&#46; The animals are placed in the body plethysmograph&#44; while the head protruded through a neck collar into a ventilated head exposure chamber&#46;<a class="elsevierStyleCrossRefs" href="#bib0175"><span class="elsevierStyleSup">35&#44;36</span></a> This apparatus which is attached to an amplifier is connected to a pneumotachograph and a differential pressure transducer&#44; which allows airflow measurement&#44; tidal volume&#44; minute volume and respiratory rate&#46;<a class="elsevierStyleCrossRef" href="#bib0185"><span class="elsevierStyleSup">37</span></a> These noninvasive serial measurements reduced the number of animal per study&#44; but long-term examination is not allowed due to the stress imposed by the neck collar restrained&#46; There is also a unrestrained double-chamber instrument where the animal&#39;s head and body are separated in a chamber by a rubber collar around the neck&#44; which creates two environments&#58; nasal chamber and thoracic chamber&#46; In single-chamber whole-body plethysmograph&#44; no restraint is required and the animals have free access to water and food&#46; These recordings may be done all day long but physiological measurement is indirect since it is done based on pressure changes in single-chamber whole-body plethysmograph&#46;<a class="elsevierStyleCrossRef" href="#bib0190"><span class="elsevierStyleSup">38</span></a> The results are reproducible despite of the chamber plethysmograph employed&#46; The single-chamber is appropriate to measure airway resistance reactivity to drug administration&#44; but not to evaluate tidal volume&#46; On other side&#44; the double-chamber is to provide accurate results about tidal volume and respiratory rate&#46;<a class="elsevierStyleCrossRef" href="#bib0185"><span class="elsevierStyleSup">37</span></a> When this technique is performed in a double chamber with restrained animal records of inspiratory and expiratory flows&#44; tidal volume&#44; minute volume and respiratory rate can be done directly&#44; but it is not possible to measure transpulmonary pressure or airway resistance&#46; The noninvasive measurements with unrestrained animal in a single chamber allow physiological measurement based on pressure alterations inside the chamber&#44; thus the inspiratory and expiratory flows are evaluated indirectly&#44; and the transpulmonary pressure cannot be analyzed&#46; The limitation is the shortage of accuracy on the evaluation of physiological parameters&#44; such as respiratory rate and tidal volume&#46;<a class="elsevierStyleCrossRef" href="#bib0195"><span class="elsevierStyleSup">39</span></a></p><p id="par0150" class="elsevierStylePara elsevierViewall">In addition to the reasons described above&#44; that justify the use of treadmill exercise to assess the functional limitations of the respiratory system induced by emphysema&#44;<a class="elsevierStyleCrossRefs" href="#bib0145"><span class="elsevierStyleSup">29&#8211;31</span></a> there is the fact that acute physical exercise is known to markedly increase the blood flow and oxygen uptake in the lung&#46; The severity of pulmonary parenchyma lesions on exercise performance can be easily evaluated by ergometric tests&#44; and in experimental models of emphysema this fact may be evaluated by testing exercise capacity in treadmill&#44; as performed by L&#252;thje et al&#46;&#44;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> which demonstrated a reduction in relative run distance in emphysematous mice&#46; Here&#44; not only the distance run and exercise time were measured but the consumption of oxygen and carbon dioxide were evaluated&#46; The results obtained demonstrated that emphysema induction committed the exercise capacity probably due to an impairment in gas exchange &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a> and <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46;</p><p id="par0155" class="elsevierStylePara elsevierViewall">In our work&#44; with a single intratracheal administration of elastase and a small degree of inclination in the treadmill&#44; we observed a reduction in running distance of 30&#46;5&#37; when compared to pre-treatment exercise capacity of emphysematous mice&#46; The greatest decrease in maximum velocity was also seen in the group treated with elastase by intratracheal route &#40;<a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46; A reduction in maximum velocity during treadmill exercise was also observed in a rat model of emphysema after intratracheal administration of elastase&#46;<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">34</span></a> The treadmill performance of mice in the study by L&#252;thje and co-workers revealed that there was no difference in running distance between control and single intratracheal administration of elastase groups&#44; whereas the reduction in running distance in the group with repetitive intratracheal administration of elastase was 29&#46;7&#37;&#46; Therefore&#44; although the pulmonary tissue destruction was more severe in the study by L&#252;thje and co-workers&#44; as shown by liner intercept length&#44; probably due to strain susceptibility and the number of elastase administration&#44; our experimental procedure resulted in the same level of serious functional impairment&#44; as revealed by the exercise performance&#46; Different from the previous works&#44; we analysed the dynamics of gas exchange during exercise&#46; The augment in oxygen consumption &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>A&#41; could reflect the ability of the heart to increase cardiac output in order to attend the metabolic demands of animal during physical effort&#44; trying to hold the blood oxygen partial pressure in the face of blood carbon dioxide partial pressure altered&#46; The maintenance of carbon dioxide production &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>B&#41; could mean the impairment of gas exchange&#46; These respiratory dysfunction is reflected in a lower exercise capacity and could be consequence of pulmonary parenchyma lesion&#46;</p><p id="par0160" class="elsevierStylePara elsevierViewall">Up to date&#44; all of the studies inducing emphysema in animal models are insufficiently capable of reproducing the slow and progressive inflammatory process that leads to chronic pulmonary injury in humans&#46; However&#44; they helped to elucidate the pathogenic mechanisms and the development of new clinical therapeutic protocols&#46; Different animals such as hamsters&#44; rats&#44; and mice were used to establish models of emphysema&#44; which are based on genetic manipulations &#40;in mice&#41;&#44; exposure to cigarette smoke&#44; or administration of proteinases into the airway&#46; Knockout or transgenic mice models for emphysema revealed different genes that could be involved in the pathogenesis of emphysema&#44; such as platelet derived growth factor A&#44;<a class="elsevierStyleCrossRef" href="#bib0200"><span class="elsevierStyleSup">40</span></a> fibroblast growth factor receptors 3 and 4&#44;<a class="elsevierStyleCrossRef" href="#bib0205"><span class="elsevierStyleSup">41</span></a> metalloproteinase-1 transgenic mice&#44;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">15</span></a> platelet derived growth factor B transgenic mice&#44;<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">42</span></a> macrophage elastase&#44;<a class="elsevierStyleCrossRef" href="#bib0215"><span class="elsevierStyleSup">43</span></a> and neutrophil elastase&#44;<a class="elsevierStyleCrossRef" href="#bib0220"><span class="elsevierStyleSup">44</span></a> the last ones given resistance to smoke-induced emphysema&#46; However&#44; it is noteworthy that these gene-targeted mice may have alterations in lung structure&#44; such as alveolar enlargement&#44; possibly associated to alterations in lung development instead of emphysema development due to destruction in pulmonary parenchyma&#44; since these genes are expressed throughout the tissue development&#44; growth&#44; and maturation&#46;<a class="elsevierStyleCrossRef" href="#bib0225"><span class="elsevierStyleSup">45</span></a> The smoke-induced animal models of COPD include nose-only exposure or whole body exposure&#46; This model may be developed in different animal species&#44; such as rat&#44;<a class="elsevierStyleCrossRef" href="#bib0230"><span class="elsevierStyleSup">46</span></a> guinea-pig&#44;<a class="elsevierStyleCrossRef" href="#bib0235"><span class="elsevierStyleSup">47</span></a> or mouse&#46;<a class="elsevierStyleCrossRef" href="#bib0240"><span class="elsevierStyleSup">48</span></a> The guinea-pig reproduces the most appropriate model of emphysema&#44; but there are many problems related to the cost&#44; shortage of tools to investigate molecular mechanisms&#44; and reduced number of reagent specific to this animal species&#46;<a class="elsevierStyleCrossRefs" href="#bib0020"><span class="elsevierStyleSup">4&#44;49</span></a> Rats are very resistant and if the time and volume of smoke exposure are extrapolated these animals developed in specific abnormalities&#46;<a class="elsevierStyleCrossRef" href="#bib0250"><span class="elsevierStyleSup">50</span></a> With regards to mice&#44; in addition to the advantages described above&#44; functional parameters such as compliance&#44; can be accurately measured&#46;<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">4</span></a> However&#44; the smoke-induced-emphysema model is not so reproductible&#44;<a class="elsevierStyleCrossRefs" href="#bib0255"><span class="elsevierStyleSup">51&#44;52</span></a> the time for developing the disease is long &#40;about six months&#41;&#44; produce only a mild disease and after the cessation of smoke the lesions do not appear to progress&#46;<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">4</span></a> Currently&#44; mice are considered the gold standard to reproduce and study many human diseases&#44; since the mouse genome shows a great similarity to the human genome&#44; having about 300 genes uniquely present in one species or the other&#46;<a class="elsevierStyleCrossRef" href="#bib0265"><span class="elsevierStyleSup">53</span></a> Furthermore&#44; more than 10&#44;000 genetic markers have been mapped in the mouse&#44; a large number of inbred strains and transgenic mice are available&#44; and data on the animals&#8217; anatomy&#44; biology&#44; immunology&#44; and physiology are available&#46;</p><p id="par0165" class="elsevierStylePara elsevierViewall">The model tested in our study provides some advantages&#44; including the use of a lower elastase concentration when compared to other studies&#46; Nonetheless&#44; it induced severe structural abnormalities that may be responsible for functional disturbances characterizing pulmonary emphysema&#46; Furthermore&#44; the emphysema could be induced with a very low mortality rate and a high degree of reproducibility&#44; which could be functional analyzed by non-invasive technique that reflects the physical impairment associated to structural abnormalities characteristic of pulmonary emphysema&#46;</p></span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Conflicts of interest</span><p id="par0170" class="elsevierStylePara elsevierViewall">The authors have no conflicts of interest to declare&#46;</p></span></span>"
    "textoCompletoSecciones" => array:1 [
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        0 => array:2 [
          "identificador" => "xres547591"
          "titulo" => "Abstract"
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          "identificador" => "xpalclavsec565468"
          "titulo" => "Keywords"
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        2 => array:2 [
          "identificador" => "xres547590"
          "titulo" => "Resumo"
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          "titulo" => "Palavras-chave"
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        4 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Introduction"
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        5 => array:3 [
          "identificador" => "sec0010"
          "titulo" => "Materials and methods"
          "secciones" => array:6 [
            0 => array:2 [
              "identificador" => "sec0015"
              "titulo" => "Animals"
            ]
            1 => array:2 [
              "identificador" => "sec0020"
              "titulo" => "Experimental groups"
            ]
            2 => array:2 [
              "identificador" => "sec0025"
              "titulo" => "Emphysema induction"
            ]
            3 => array:2 [
              "identificador" => "sec0030"
              "titulo" => "Treadmill"
            ]
            4 => array:2 [
              "identificador" => "sec0035"
              "titulo" => "Histopathological analysis"
            ]
            5 => array:2 [
              "identificador" => "sec0040"
              "titulo" => "Statistical analysis"
            ]
          ]
        ]
        6 => array:3 [
          "identificador" => "sec0045"
          "titulo" => "Results"
          "secciones" => array:3 [
            0 => array:2 [
              "identificador" => "sec0050"
              "titulo" => "Safety of the procedure &#8211; mortality"
            ]
            1 => array:2 [
              "identificador" => "sec0055"
              "titulo" => "Functional analysis &#8211; ergometry"
            ]
            2 => array:2 [
              "identificador" => "sec0060"
              "titulo" => "Morphometric analysis &#8211; mean linear interseptum measurement"
            ]
          ]
        ]
        7 => array:2 [
          "identificador" => "sec0065"
          "titulo" => "Discussion and conclusion"
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        8 => array:2 [
          "identificador" => "sec0070"
          "titulo" => "Conflicts of interest"
        ]
        9 => array:2 [
          "identificador" => "xack184907"
          "titulo" => "Acknowledgements"
        ]
        10 => array:1 [
          "titulo" => "References"
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      ]
    ]
    "pdfFichero" => "main.pdf"
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    "fechaRecibido" => "2011-07-06"
    "fechaAceptado" => "2011-12-23"
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        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec565468"
          "palabras" => array:4 [
            0 => "Pulmonary emphysema"
            1 => "Ergometric test"
            2 => "Porcine elastase"
            3 => "C57Bl&#47;6"
          ]
        ]
      ]
      "pt" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palavras-chave"
          "identificador" => "xpalclavsec565469"
          "palabras" => array:4 [
            0 => "Enfisema pulmonar"
            1 => "ensaio ergom&#233;trico"
            2 => "Elastase su&#237;na"
            3 => "C57Bl&#47;6"
          ]
        ]
      ]
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    "resumen" => array:2 [
      "en" => array:2 [
        "titulo" => "Abstract"
        "resumen" => "<span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Several experimental studies of pulmonary emphysema using animal models have been described in the literature&#46; However&#44; only a few of these studies have focused on the assessment of ergometric function as a non-invasive technique to validate the methodology used for induction of experimental emphysema&#46; Additionally&#44; functional assessments of emphysema are rarely correlated with morphological pulmonary abnormalities caused by induced emphysema&#46; The present study aimed to evaluate the effects of elastase administered by tracheal puncture on pulmonary parenchyma and their corresponding functional impairment&#46; This was evaluated by measuring exercise capacity in C57Bl&#47;6 mice in order to establish a reproducible and safe methodology of inducing experimental emphysema&#46; Thirty six mice underwent ergometric tests before and 28 days after elastase administration&#46; Pancreatic porcine elastase solution was administered by tracheal puncture&#44; which resulted in a significantly decreased exercise capacity&#44; shown by a shorter distance run &#40;&#8722;30&#46;5&#37;&#41; and a lower mean velocity &#40;&#8722;15&#37;&#41;&#44; as well as in failure to increase the elimination of carbon dioxide&#46; The mean linear intercept increased significantly by 50&#37; in tracheal elastase administration&#46; In conclusion&#44; application of elastase by tracheal function in C57Bl&#47;6 induces emphysema&#44; as validated by morphometric analyses&#44; and resulted in a significantly lower exercise capacity&#44; while resulting in a low mortality rate&#46;</p></span>"
      ]
      "pt" => array:2 [
        "titulo" => "Resumo"
        "resumen" => "<span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">V&#225;rios estudos experimentais de enfisema pulmonar em modelos animais t&#234;m sido descritos na literatura cient&#237;fica&#46; No entanto&#44; apenas alguns destes estudos t&#234;m sido concentrados na avalia&#231;&#227;o da fun&#231;&#227;o ergom&#233;trica como t&#233;cnica n&#227;o-invasiva para validar a metodologia utilizada para a indu&#231;&#227;o do enfisema experimental&#46; Al&#233;m disso&#44; as avalia&#231;&#245;es funcionais de enfisema raramente se encontram correlacionadas com anomalias morfol&#243;gicas pulmonares causadas por enfisema induzido&#46; O presente estudo teve como objetivo avaliar os efeitos da elastase administrada por pun&#231;&#227;o traqueal no par&#234;nquima pulmonar e a sua disfun&#231;&#227;o funcional correspondente&#46; Esta foi avaliada atrav&#233;s da medi&#231;&#227;o da capacidade de exerc&#237;cio em ratos C57Bl&#47;6&#44; de forma a estabelecer uma metodologia reprodut&#237;vel e segura de induzir o enfisema experimental&#46; Trinta e seis ratos foram submetidos a testes ergom&#233;tricos antes e 28 dias ap&#243;s a administra&#231;&#227;o de elastase&#46; A solu&#231;&#227;o de elastase pancre&#225;tica su&#237;na foi administrada por pun&#231;&#227;o traqueal&#44; o que resultou numa diminui&#231;&#227;o significativamente da capacidade de exerc&#237;cio&#44; demonstrada pela diminui&#231;&#227;o da dist&#226;ncia percorrida &#40;menos de 30&#44;5&#37;&#41; e por uma velocidade m&#233;dia inferior &#40;menos de 15&#37;&#41;&#44; assim como pela incapacidade de aumentar a elimina&#231;&#227;o de di&#243;xido de carbono&#46; A intersec&#231;&#227;o linear m&#233;dia aumentou significativamente em 50&#37; na administra&#231;&#227;o traqueal da elastase&#46; Em conclus&#227;o&#44; a aplica&#231;&#227;o de elastase por pun&#231;&#227;o traqueal em ratos C57Bl&#47;6 induz enfisema&#44; conforme foi validado por an&#225;lises morfom&#233;tricas&#44; e resultou numa capacidade de exerc&#237;cio significativamente mais baixa&#44; embora se tenha obtido uma baixa taxa de mortalidade&#46;</p></span>"
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          "en" => "<p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Comparison of respiratory function during physical effort in mice before and after the administration of elastase&#46; Oxygen consumption &#40;A&#41; and carbon dioxide release &#40;B&#41;&#44; during resting conditions&#44; exercising at 5 different velocities &#40;7&#46;2&#44; 14&#46;4&#44; 21&#46;6&#44; 28&#46;8 and 36<span class="elsevierStyleHsp" style=""></span>m&#47;min&#41; on a motorized treadmill&#44; immediately &#40;Exhaustion&#41; and 25<span class="elsevierStyleHsp" style=""></span>min after exercise &#40;recovery&#41;&#46; Data are means<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SD&#59; 10<span class="elsevierStyleHsp" style=""></span>mice&#47;group&#46; Note that mice elastase-treated were unable to run at a velocity of 36<span class="elsevierStyleHsp" style=""></span>m&#47;min&#59; therefore&#44; no data are given&#46; &#42;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#59; &#42;&#42;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01&#59; &#42;&#42;&#42;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#46;</p>"
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          "en" => "<p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Comparison of the mean linear intercept length in three groups of C57Bl&#47;6 mice treated in &#40;A&#41; non-manipulated animals &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>12&#41;&#44; &#40;B&#41; animals receiving tracheal elastase &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>12&#41;&#44; or &#40;C&#41; receiving tracheal saline &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>12&#41;&#46; Data are presented as means<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SD&#46; &#42; Indicates a significant difference &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#41; between goups&#46;</p>"
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                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Group&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Number of mice&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Mortality&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">&#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">A&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">12&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">B&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">12&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">16&#46;7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">C&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">12&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">16&#46;7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="4" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">Total&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">36&nbsp;\t\t\t\t\t\t\n
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          "en" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Mice performance during exercise on a motorized treadmill evaluated by maximum velocity reached and maximum distance covered before &#40;pre&#41; and after treatment &#40;post&#41; and the respective percentage change &#40;&#37;&#41;&#46; Groups as in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#46;</p>"
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                            0 => "M&#46;D&#46; O&#8217;Donnell"
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        "texto" => "<p id="par0180" class="elsevierStylePara elsevierViewall">This work was supported by grants from the <span class="elsevierStyleGrantSponsor">Brazilian Ministry of Science and Technology</span>&#44; <span class="elsevierStyleGrantSponsor">CNPq</span>&#44; <span class="elsevierStyleGrantSponsor">FINEP</span>&#44; <span class="elsevierStyleGrantSponsor">FIOCRUZ</span>&#44; and <span class="elsevierStyleGrantSponsor">FAPESB</span>&#46;</p>"
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ISSN: 08732159
Original language: English
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