Artigo Original/Original Article
Tuberculose multirresistente: Detecção directa em amostras respiratórias com o método de genética molecular MTBDRplus®
Multidrug-resistant tuberculosis: Rapid molecular detection with MTBDRplus® assay in clinical samples
Rita Macedo1,
, António Amorim1, Edna Pereira1
Corresponding author
rita.macedo@csalcantara.min-saude.pt
Correspondência / Correspondence to: Laboratório de Saúde Pública – Micobacteriologia/ Tuberculose, Administração Regional de Saúde de Lisboa e Vale do Tejo, I.P., Rua Pedro Cálmon, n.º 25, 1300-455 Lisboa Tel: +351 213602520 Fax: +351 213602525
Correspondência / Correspondence to: Laboratório de Saúde Pública – Micobacteriologia/ Tuberculose, Administração Regional de Saúde de Lisboa e Vale do Tejo, I.P., Rua Pedro Cálmon, n.º 25, 1300-455 Lisboa Tel: +351 213602520 Fax: +351 213602525
1 Laboratório de Saúde Pública: Micobacteriologia/ Tuberculose, Administração Regional de Saúde de Lisboa e Vale do Tejo, IP/Public Health Laboratory: Mycobacteriology/Tuberculosis, Regional Health Administration, Lisbon and Tagus Valley Health Authority
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A detecção precoce da resistência a fármacos, directamente a partir de amostras respiratórias, é essencial para que se assegure o tratamento atempado, adequado e eficaz da tuberculose, bem como para prevenir a disseminação destes casos de especial gravidade.</p><p id="sp0010" class="elsevierStyleSimplePara elsevierViewall">O nosso objectivo foi avaliar a sensibilidade e comparar os resultados obtidos com um método de genética molecular disponível comercialmente – MTBDRplus® – e o isolamento, identificação e testes de sensibilidade clássicos, directamente a partir de amostras respiratórias. Estudamos 68 amostras, com baciloscopia positiva.</p><p id="sp0015" class="elsevierStyleSimplePara elsevierViewall">O MTBDRplus® permitiu identificar, directamente a partir das amostras respiratórias, o complexo <span class="elsevierStyleItalic">Mycobacte</span>-<span class="elsevierStyleItalic">rium tuberculosis</span> em todos os casos em que o estudo cultural e a identificação clássica chegaram a esse mesmo resultado. Nas amostras em que culturalmente iso-lámos estirpes sensíveis, com o MTBDRplus® encontrá-mos sempre perfis genéticos do tipo selvagem (63,2%). Relativamente às amostras em que culturalmente isolá-mos estirpes resistentes, com o MTBDRplus® encontrámos sempre perfis genéticos com mutações ou com au-sência do perfil do tipo selvagem (36,8%).</p><p id="sp0020" class="elsevierStyleSimplePara elsevierViewall">Este estudo permitiu concluir que o MTBDRplus® assegura a detecção rápida de resistências a fármacos em estirpes do complexo <span class="elsevierStyleItalic">M. tuberculosis</span>, com resultados totalmente sobreponíveis aos obtidos com os mé-todos bacteriológicos clássicos.</p><p id="sp0025" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleBold">Rev Port Pneumol 2009; XV (3): 353-366</span></p></span>" ] "en" => array:2 [ "titulo" => "Abstract" "resumen" => "<span id="as0010" class="elsevierStyleSection elsevierViewall"><p id="sp0030" class="elsevierStyleSimplePara elsevierViewall">Nowadays, the greatest concern of tuberculosis control programmes is the appearance of multidrug-resistant tuberculosis and extensively drug-resistant tuberculosis. Rapid determination of drug resistance in clinical samples, with <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex (MTC), is the prerequisite for initiating effective chemotherapy, ensuring successful treatment of the patient and preventing further spread of drug-resistant isolates.</p><p id="sp0035" class="elsevierStyleSimplePara elsevierViewall">The aim of our study was to determine the sensitivity of the new MTBDRplus® assay in comparison to culture, identification and classic DST, directly from smear-positive clinical specimens.</p><p id="sp0040" class="elsevierStyleSimplePara elsevierViewall">A total of 68 smear-positive sputum specimens were processed by both the classical mycobacteriological methods and the molecular assay, MTBDRplus®.</p><p id="sp0045" class="elsevierStyleSimplePara elsevierViewall">MTBDRplus® assay allowed an accurate identification of MTC species by detection of the specific band in all samples, from which we also isolated and identified MTC strains by culture methods. In the samples from which we isolated susceptible strains (63.2%), wild type patterns were found using MTB-DRplus® assay. The samples from which we isolated resistant strains (36.8%) showed specific mutations associated with the correspondent resistant pheno-type.</p><p id="sp0050" class="elsevierStyleSimplePara elsevierViewall">Our study indicated that this assay allows rapid detection of resistance, always in agreement with classic methods.</p><p id="sp0055" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleBold">Rev Port Pneumol 2009; XV (3): 353-366</span></p></span>" ] ] "lecturaRecomendada" => array:1 [ 0 => array:3 [ "vista" => "all" "titulo" => "<span class="elsevierStyleSectionTitle" id="st0025">Bibliografia/Bibliography</span>" "seccion" => array:1 [ 0 => array:2 [ "vista" => "all" "bibliografiaReferencia" => array:23 [ 0 => array:3 [ "identificador" => "bb0005" "etiqueta" => "1." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Guidelines for the programmatic management of drug resistant tuberculosis. WHO/HTM/TB/2006.361" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "World Health Organization" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "2006" "editorial" => "World Health Organization" "editorialLocalizacion" => "Geneva" ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bb0010" "etiqueta" => "2." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:1 [ "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "SVIG-TB, Direcção-Geral da Saúde" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:2 [ "titulo" => "Ponto da situa-ção epidemiológica e de desempenho em 2007, dados preliminares" "fecha" => "2007" ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bb0015" "etiqueta" => "3." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:1 [ "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "World Health Organization" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:2 [ "titulo" => "Antituberculous drug resistance in the World, Report number 3" "fecha" => "2005" ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bb0020" "etiqueta" => "4." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Epidemiology of antituberculosis drug resistance (the Global Project on Anti-tuberculosis Drug Resistance Surveillance) an updated analysis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:10 [ 0 => "M.A. Aziz" 1 => "A. Wright" 2 => "A. Laszlo" 3 => "A. de Muuynck" 4 => "F. Portaels" 5 => "A. van Deun" 6 => "C. Wells" 7 => "P. Nunn" 8 => "L. Blanc" 9 => "M. Raviglione" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/S0140-6736(06)69863-2" "Revista" => array:6 [ "tituloSerie" => "Lancet" "fecha" => "2006" "volumen" => "368" "paginaInicial" => "2142" "paginaFinal" => "2154" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17174706" "web" => "Medline" ] ] ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bb0025" "etiqueta" => "5." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Multidrug–resistant tuberculosis and its control" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "A. Kochi" 1 => "B. Vareldzis" 2 => "K. Styblo" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Res Microbiol" "fecha" => "1993" "volumen" => "144" "paginaInicial" => "104" "paginaFinal" => "110" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8337467" "web" => "Medline" ] ] ] ] ] ] ] ] 5 => array:3 [ "identificador" => "bb0030" "etiqueta" => "6." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "XDR Tuberculosis – Implications for global public health" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "M.C. Raviglione" 1 => "M.B. Smith" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1056/NEJMp068273" "Revista" => array:6 [ "tituloSerie" => "N Engl J Med" "fecha" => "2007" "volumen" => "356" "paginaInicial" => "656" "paginaFinal" => "659" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17301295" "web" => "Medline" ] ] ] ] ] ] ] ] 6 => array:3 [ "identificador" => "bb0035" "etiqueta" => "7." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Addressing the threat of tuberculosis caused by extensively drug resistant <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> (XDR-TB)" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "World Health Organization" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Weekly Epidemiological Record" "fecha" => "2006" "volumen" => "81" "paginaInicial" => "385" "paginaFinal" => "396" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17042080" "web" => "Medline" ] ] ] ] ] ] ] ] 7 => array:3 [ "identificador" => "bb0040" "etiqueta" => "8." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Laboratory diagnostic aspects of drug resistant tuberculosis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "L.M. Parsons" 1 => "A. Somoskövi" 2 => "R. Urbanczik" 3 => "M. Salfinger" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Front Biosci" "fecha" => "2004" "volumen" => "9" "paginaInicial" => "2086" "paginaFinal" => "2105" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15353272" "web" => "Medline" ] ] ] ] ] ] ] ] 8 => array:3 [ "identificador" => "bb0045" "etiqueta" => "9." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Fármacos con actividad frente a <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "P. Coll" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Enferm Infecc Microbiol Clin" "fecha" => "2003" "volumen" => "21" "paginaInicial" => "299" "paginaFinal" => "308" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12809585" "web" => "Medline" ] ] ] ] ] ] ] ] 9 => array:3 [ "identificador" => "bb0050" "etiqueta" => "10." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The molecular basis of resistance to isoniazid, rifampin and pyrazinamide in <span class="elsevierStyleItalic">My</span>-<span class="elsevierStyleItalic">cobacterium tuberculosis</span>" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "A. Somoskovi" 1 => "L.M. Parsons" 2 => "M. Salfinger" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Respir Res" "fecha" => "2001" "volumen" => "2" "paginaInicial" => "164" "paginaFinal" => "168" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11686881" "web" => "Medline" ] ] ] ] ] ] ] ] 10 => array:3 [ "identificador" => "bb0055" "etiqueta" => "11." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Evidence for the generation of active oxygen by isoniazid treatment of extracts of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> H37Ra" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "H.A. Shoeb" 1 => "B.U. Bowman Jr." 2 => "A.C. Ottolenghi" 3 => "A.J. Merola" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Antimicrob Agents Chemother" "fecha" => "1985" "volumen" => "27" "paginaInicial" => "404" "paginaFinal" => "407" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2986545" "web" => "Medline" ] ] ] ] ] ] ] ] 11 => array:3 [ "identificador" => "bb0060" "etiqueta" => "12." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Effect of katG mutations on the virulence of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> and the implication for transmission in humans" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "A.S. Pym" 1 => "B. Saint-Joanis" 2 => "S.T. Cole" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Infect Immun" "fecha" => "2002" "volumen" => "70" "paginaInicial" => "4955" "paginaFinal" => "4960" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12183541" "web" => "Medline" ] ] ] ] ] ] ] ] 12 => array:3 [ "identificador" => "bb0065" "etiqueta" => "13." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Expression of katG in <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> is associated with its growth and persistence in mice and guinea pigs" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "Z. Li" 1 => "C. Kelley" 2 => "F. Collins" 3 => "D. Rouse" 4 => "S. Morris" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "J Infect Dis" "fecha" => "1998" "volumen" => "177" "paginaInicial" => "1030" "paginaFinal" => "1035" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9534978" "web" => "Medline" ] ] ] ] ] ] ] ] 13 => array:3 [ "identificador" => "bb0070" "etiqueta" => "14." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "InhA, a gene encoding a target for isoniazid and ethionamide in <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:9 [ 0 => "A. Banerjee" 1 => "E. Dubnau" 2 => "A. Quemard" 3 => "V. Balasubramanian" 4 => "K.S. Um" 5 => "T. Wilson" 6 => "D. Collins" 7 => "G. de Lisle" 8 => "W.R. Jacobs Jr." ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Science" "fecha" => "1994" "volumen" => "263" "paginaInicial" => "227" "paginaFinal" => "230" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8284673" "web" => "Medline" ] ] ] ] ] ] ] ] 14 => array:3 [ "identificador" => "bb0075" "etiqueta" => "15." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "MDR--TB in Lisbon area: data report from 2004 to 2006" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "R. Macedo" 1 => "J. Perdigão" 2 => "I. Portugal" 3 => "L. Brum" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "titulo" => "28th Annual Congress of European Society of Myco-bacteriology (Abstract book)" "fecha" => "2007" "paginaInicial" => "37" ] ] ] ] ] ] 15 => array:3 [ "identificador" => "bb0080" "etiqueta" => "16." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Comparison of two commercially available DNA line probe assays for detection of multidrug–resistant Mycobacterium tuberculosis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "J. Mäkinen" 1 => "H.J. Marttila" 2 => "M. Marjamäki" 3 => "M.K. Viljanen" 4 => "H. Soini" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1128/JCM.44.2.350-352.2006" "Revista" => array:6 [ "tituloSerie" => "J Clin Microbiol" "fecha" => "2006" "volumen" => "44" "paginaInicial" => "350" "paginaFinal" => "352" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16455882" "web" => "Medline" ] ] ] ] ] ] ] ] 16 => array:3 [ "identificador" => "bb0085" "etiqueta" => "17." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Current perspectives on drug susceptibility testing of Mycobacterium tuberculosis complex: the automated nonra-diometric systems" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "C. Piersimoni" 1 => "A. Olivieri" 2 => "L. Benacchio" 3 => "C. Scarparo" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1128/JCM.44.1.20-28.2006" "Revista" => array:6 [ "tituloSerie" => "J Clin Microbiol" "fecha" => "2006" "volumen" => "44" "paginaInicial" => "20" "paginaFinal" => "28" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16390942" "web" => "Medline" ] ] ] ] ] ] ] ] 17 => array:3 [ "identificador" => "bb0090" "etiqueta" => "18." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Rapid genotypic detection of rifampin– and isoniazid-resistant <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> directly in clinical specimens" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "D. Bang" 1 => "A. Bengård Andersen" 2 => "V.Ø. Thomsen" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1128/JCM.00752-06" "Revista" => array:6 [ "tituloSerie" => "J Clin Microbiol" "fecha" => "2006" "volumen" => "44" "paginaInicial" => "2605" "paginaFinal" => "2608" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16825393" "web" => "Medline" ] ] ] ] ] ] ] ] 18 => array:3 [ "identificador" => "bb0095" "etiqueta" => "19." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Evaluation of the genotype MTBDR assay for rapid detection of rifampin and isoniazid resistance in <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> isolates" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "C. Cavusoglu" 1 => "A. Turhan" 2 => "P. Akinci" 3 => "I. Soyler" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1128/JCM.00425-06" "Revista" => array:6 [ "tituloSerie" => "J Clin Microbiol" "fecha" => "2006" "volumen" => "44" "paginaInicial" => "2338" "paginaFinal" => "2342" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16825346" "web" => "Medline" ] ] ] ] ] ] ] ] 19 => array:3 [ "identificador" => "bb0100" "etiqueta" => "20." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Use of the genotype MTBDR assay for rapid detection of rifampin and isoniazid resistance in <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex isolates" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "D. Hillemann" 1 => "M. Weizenegger" 2 => "T. Kubica" 3 => "E. Richter" 4 => "S. Niemann" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1128/JCM.43.8.3699-3703.2005" "Revista" => array:6 [ "tituloSerie" => "J Clin Microbiol" "fecha" => "2005" "volumen" => "43" "paginaInicial" => "3699" "paginaFinal" => "3703" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16081898" "web" => "Medline" ] ] ] ] ] ] ] ] 20 => array:3 [ "identificador" => "bb0105" "etiqueta" => "21." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Use of genotype MTBDR assay for molecular detection of rifampin and isoniazid resistance in <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> clinical strains isolated in Italy" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "P. Miotto" 1 => "F. Piana" 2 => "V. Penati" 3 => "F. Canducci" 4 => "G.B. Migliori" 5 => "D.M. Cirillo" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1128/JCM.00083-06" "Revista" => array:6 [ "tituloSerie" => "J Clin Microbiol" "fecha" => "2006" "volumen" => "44" "paginaInicial" => "2485" "paginaFinal" => "2491" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16825369" "web" => "Medline" ] ] ] ] ] ] ] ] 21 => array:3 [ "identificador" => "bb0110" "etiqueta" => "22." 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Article information
ISSN: 08732159Original language: Portuguese
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