array:24 [
  "pii" => "S0873215915302555"
  "issn" => "08732159"
  "doi" => "10.1016/S0873-2159(15)30255-5"
  "estado" => "S350"
  "fechaPublicacion" => "2008-07-01"
  "aid" => "3"
  "copyright" => "Sociedade Portuguesa de Pneumologia/SPP"
  "copyrightAnyo" => "2008"
  "documento" => "simple-article"
  "crossmark" => 0
  "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
  "subdocumento" => "fla"
  "cita" => "Rev Port Pneumol. 2008;14:509-16"
  "abierto" => array:3 [
    "ES" => true
    "ES2" => true
    "LATM" => true
  ]
  "gratuito" => true
  "lecturas" => array:2 [
    "total" => 2028
    "formatos" => array:3 [
      "EPUB" => 238
      "HTML" => 1133
      "PDF" => 657
    ]
  ]
  "itemSiguiente" => array:19 [
    "pii" => "S0873215915302579"
    "issn" => "08732159"
    "doi" => "10.1016/S0873-2159(15)30257-9"
    "estado" => "S350"
    "fechaPublicacion" => "2008-07-01"
    "aid" => "5"
    "copyright" => "Sociedade Portuguesa de Pneumologia/SPP"
    "documento" => "simple-article"
    "crossmark" => 0
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "fla"
    "cita" => "Rev Port Pneumol. 2008;14:527-34"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 2868
      "formatos" => array:3 [
        "EPUB" => 212
        "HTML" => 1367
        "PDF" => 1289
      ]
    ]
    "pt" => array:11 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Caso Cl&#237;nico&#47;Clinical Case</span>"
      "titulo" => "Revis&#227;o dos tumores da traqueia &#8211; A prop&#243;sito de um caso cl&#237;nico de tumor aden&#243;ide c&#237;stico"
      "tienePdf" => "pt"
      "tieneTextoCompleto" => 0
      "tieneResumen" => array:2 [
        0 => "pt"
        1 => "en"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "527"
          "paginaFinal" => "534"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "en" => array:1 [
          "titulo" => "Tracheal tumors review &#8211; A clinical case of adenoid cystic carcinoma"
        ]
      ]
      "contieneResumen" => array:2 [
        "pt" => true
        "en" => true
      ]
      "contienePdf" => array:1 [
        "pt" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Ant&#243;nio Caiado, Jo&#227;o Moura e S&#225;"
          "autores" => array:2 [
            0 => array:2 [
              "nombre" => "Ant&#243;nio"
              "apellidos" => "Caiado"
            ]
            1 => array:2 [
              "nombre" => "Jo&#227;o"
              "apellidos" => "Moura e S&#225;"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "pt"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0873215915302579?idApp=UINPBA00004E"
    "url" => "/08732159/0000001400000004/v1_201509151547/S0873215915302579/v1_201509151547/pt/main.assets"
  ]
  "itemAnterior" => array:19 [
    "pii" => "S0873215915302567"
    "issn" => "08732159"
    "doi" => "10.1016/S0873-2159(15)30256-7"
    "estado" => "S350"
    "fechaPublicacion" => "2008-07-01"
    "aid" => "4"
    "copyright" => "Sociedade Portuguesa de Pneumologia&#47;SPP"
    "documento" => "simple-article"
    "crossmark" => 0
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "rev"
    "cita" => "Rev Port Pneumol. 2008;14:517-26"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 2932
      "formatos" => array:3 [
        "EPUB" => 231
        "HTML" => 1738
        "PDF" => 963
      ]
    ]
    "pt" => array:11 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Artigo de Revis&#227;o&#47;Revision Article</span>"
      "titulo" => "Tosse em pediatria"
      "tienePdf" => "pt"
      "tieneTextoCompleto" => 0
      "tieneResumen" => array:2 [
        0 => "pt"
        1 => "en"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "517"
          "paginaFinal" => "526"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "en" => array:1 [
          "titulo" => "Cough in paediatrics"
        ]
      ]
      "contieneResumen" => array:2 [
        "pt" => true
        "en" => true
      ]
      "contienePdf" => array:1 [
        "pt" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Susana Martins, Marta Carneiro de Moura, Ana Margarida Neves, Jos&#233; Costa Trindade"
          "autores" => array:4 [
            0 => array:2 [
              "nombre" => "Susana"
              "apellidos" => "Martins"
            ]
            1 => array:2 [
              "nombre" => "Marta Carneiro"
              "apellidos" => "de Moura"
            ]
            2 => array:2 [
              "nombre" => "Ana Margarida"
              "apellidos" => "Neves"
            ]
            3 => array:2 [
              "nombre" => "Jos&#233; Costa"
              "apellidos" => "Trindade"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "pt"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0873215915302567?idApp=UINPBA00004E"
    "url" => "/08732159/0000001400000004/v1_201509151547/S0873215915302567/v1_201509151547/pt/main.assets"
  ]
  "pt" => array:15 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Do Laborat&#243;rio &#224; Pr&#225;tica Cl&#237;nica&#47;From Laboratory to Clinical Practice</span>"
    "titulo" => "Utiliza&#231;&#227;o estrat&#233;gica da genotipagem do <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> no controlo da tuberculose"
    "tieneTextoCompleto" => 0
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "509"
        "paginaFinal" => "516"
      ]
    ]
    "autores" => array:1 [
      0 => array:3 [
        "autoresLista" => "Susana David"
        "autores" => array:1 [
          0 => array:4 [
            "nombre" => "Susana"
            "apellidos" => "David"
            "email" => array:1 [
              0 => "sdavid&#64;ibmc&#46;up&#46;pt"
            ]
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">1</span>"
                "identificador" => "af0005"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:1 [
          0 => array:3 [
            "entidad" => "Investigadora Principal&#44; Centro de Tuberculose e Micobact&#233;rias&#44; Instituto Nacional de Sa&#250;de Dr&#46; Ricardo Jorge&#44; Rua do Campo Alegre 823&#44; 4150-180 Porto"
            "etiqueta" => "1"
            "identificador" => "af0005"
          ]
        ]
      ]
    ]
    "titulosAlternativos" => array:1 [
      "en" => array:1 [
        "titulo" => "<span class="elsevierStyleItalic">Strategical use of genotyping of</span> Mycobacterium tuberculosis <span class="elsevierStyleItalic">in tuberculosis control</span>"
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2008-01-30"
    "fechaAceptado" => "2008-03-26"
    "PalabrasClave" => array:2 [
      "pt" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palavras-chave"
          "identificador" => "xpalclavsec570532"
          "palabras" => array:3 [
            0 => "<span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>"
            1 => "Epidemiologia molecular"
            2 => "genotipagem"
          ]
        ]
      ]
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Key-words"
          "identificador" => "xpalclavsec570533"
          "palabras" => array:3 [
            0 => "<span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>"
            1 => "molecular epidemiology"
            2 => "genotyping"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:2 [
      "pt" => array:2 [
        "titulo" => "Resumo"
        "resumen" => "<span id="as0005" class="elsevierStyleSection elsevierViewall"><p id="sp0005" class="elsevierStyleSimplePara elsevierViewall">A situa&#231;&#227;o da tuberculose em Portugal justifica a aplica&#231;&#227;o de uma estrat&#233;gia de genotipagem do <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>&#44; tanto mais que Portugal encontrase inserido no contexto global de mobilidade das popula&#231;&#245;es humanas e das suas consequ&#234;ncias sobre a pandemia&#46;</p><p id="sp0010" class="elsevierStyleSimplePara elsevierViewall">V&#225;rios estudos internacionais posicionam as t&#233;cnicas do <span class="elsevierStyleItalic">spoligotyping</span> e MIRU-VNTR <span class="elsevierStyleItalic">typing</span> como de primeira linha na epidemiologia molecular do <span class="elsevierStyleItalic">M&#46; tuberculosis</span> por estarem baseadas em tecnologias simples &#40;PCR&#41; e produzirem padr&#245;es&#44; podendo ser traduzidos em c&#243;digo num&#233;rico de interpreta&#231;&#227;o directa&#46; Assim&#44; tem sido proposta a aplica&#231;&#227;o do <span class="elsevierStyleItalic">spoligotyping</span> a todos os isolados cl&#237;nicos&#44; enquanto o MIRU-VNTR <span class="elsevierStyleItalic">typing</span> seria aplicado aos isolados com <span class="elsevierStyleItalic">spoligotype</span> comum&#46; Outras t&#233;cnicas&#44; incluindo o IS6110-RFLP&#44; seriam reservadas a aplica&#231;&#245;es segundo crit&#233;rios seleccionados&#46;</p><p id="sp0015" class="elsevierStyleSimplePara elsevierViewall">Os trabalhos anteriores em Portugal utilizando o <span class="elsevierStyleItalic">spoligotyping</span> apontaram para a vantagem de uma estrat&#233;gia de genotipagem baseada numa amostragem consecutiva e sem crit&#233;rio de pr&#233;-selec&#231;&#227;o&#46; Esta permite a caracteriza&#231;&#227;o da estrutura populacional do <span class="elsevierStyleItalic">M&#46; tuberculosis</span> atrav&#233;s do conhecimento da distribui&#231;&#227;o dos genotipos geograficamente&#44; no tempo e dentro dos v&#225;rios grupos de risco&#46;</p><p id="sp0020" class="elsevierStyleSimplePara elsevierViewall">Por outro lado&#44; a associa&#231;&#227;o do <span class="elsevierStyleItalic">spoligotyping</span> com o MIRU-VNTR <span class="elsevierStyleItalic">typing</span> e&#44; eventualmente&#44; outras t&#233;cnicas&#44; dever&#225; ser avaliada em v&#225;rios contextos&#44; como na identifica&#231;&#227;o de situa&#231;&#245;es de transmiss&#227;o recente&#44; na distin&#231;&#227;o entre epis&#243;dios de reinfec&#231;&#227;o e reca&#237;da&#44; na caracteriza&#231;&#227;o da amplitude e din&#226;mica de transmiss&#227;o da doen&#231;a&#46;</p><p id="sp0025" class="elsevierStyleSimplePara elsevierViewall">A solu&#231;&#227;o do problema da tuberculose em Portugal passa por uma estrutura&#231;&#227;o na utiliza&#231;&#227;o da genotipagem em apoio &#224; luta contra a tuberculose&#44; a ser avaliada atrav&#233;s de exemplos e resultados concretos&#46;</p><p id="sp0030" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleBold">Rev Port Pneumol 2008&#59; XIV &#40;4&#41;&#58; 509-516</span></p></span>"
      ]
      "en" => array:2 [
        "titulo" => "Abstract"
        "resumen" => "<span id="as0010" class="elsevierStyleSection elsevierViewall"><p id="sp0035" class="elsevierStyleSimplePara elsevierViewall">The tuberculosis situation in Portugal justifies the use of a strategy for the genotyping of <span class="elsevierStyleItalic">Mycobacterium tuberculosis&#44;</span> particularly as Portugal is part of the global backdrop of human mobility&#44; something which has a knock-on effect on the pandemic&#46;</p><p id="sp0040" class="elsevierStyleSimplePara elsevierViewall">Several international studies have placed spoligotyping and MIRU-VNTR typing as first line techniques for the molecular epidemiology of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> as these techniques rely on simple technologies &#40;PCR&#41; and produee patterns which are easily translated into a direct interpretation numerical code&#46; Spoligotyping has been accordingly proposed for all the isolates&#44; while MIRU-VNTR typing should be applied to isolates with a common spoliotype&#46; Other techniques&#44; including IS6110-RFLP&#44; should be reserved for use ill accordance with selected criteria&#46;</p><p id="sp0045" class="elsevierStyleSimplePara elsevierViewall">Previous studies in Portugal using spoligotyping have underlined the advantages of a strategy based on sampling consecutive patient isolates with no prior selection criteria&#46; This allows characterisation of the <span class="elsevierStyleItalic">M&#46; tuberculosis</span> population structure through monitoring the distribution of the genotypes geographically over time and within the various risk groups&#46;</p><p id="sp0050" class="elsevierStyleSimplePara elsevierViewall">On the other hand&#44; the association of spoligotyping&#44; MIRU-VNTF &#40;typing and&#44; possibly&#44; other techniques&#44; needs evaluating as part of bigger pictures&#44; including identifying recent transmission situations&#44; distinguishing between reinfection and relapse episodes and ma pping the size and dynamics of disease transmission&#46;</p><p id="sp0055" class="elsevierStyleSimplePara elsevierViewall">The solution to the tuberculosis problem in Portugal implies structuring genotyping&#8217;s role in tuberculosis prevention and control and its evaluation through concrete examples and results&#46;</p><p id="sp0060" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleBold">Rev Port Pneumol 2008&#59; XIV &#40;4&#41;&#58; 509-516</span></p></span>"
      ]
    ]
    "lecturaRecomendada" => array:1 [
      0 => array:3 [
        "vista" => "all"
        "titulo" => "<span class="elsevierStyleSectionTitle" id="st0025">Bibliografia</span>"
        "seccion" => array:1 [
          0 => array:2 [
            "vista" => "all"
            "bibliografiaReferencia" => array:25 [
              0 => array:3 [
                "identificador" => "bb0005"
                "etiqueta" => "1&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "<span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex genetic diversity&#58; mining the fourth international spoligotyping database &#40;SpolDB4&#41; for classification&#44; population genetics and epidemiology"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:66 [
                              0 => "K&#46; Brudey"
                              1 => "J&#46;R&#46; Driscoll"
                              2 => "L&#46; Rigouts"
                              3 => "W&#46;M&#46; Prodinger"
                              4 => "A&#46; Gori"
                              5 => "S&#46;A&#46; Al-Hajoj"
                              6 => "C&#46; Allix"
                              7 => "L&#46; Aristimuno"
                              8 => "J&#46; Arora"
                              9 => "V&#46; Baumanis"
                              10 => "L&#46; Binder"
                              11 => "P&#46; Cafrune"
                              12 => "A&#46; Cataldi"
                              13 => "S&#46; Cheong"
                              14 => "R&#46; Diel"
                              15 => "C&#46; Ellermeier"
                              16 => "J&#46;T&#46; Evans"
                              17 => "M&#46; Fauville-Dufaux"
                              18 => "S&#46; Ferdinand"
                              19 => "Viedma D&#46; Garcia de"
                              20 => "C&#46; Garzelli"
                              21 => "L&#46; Gazzola"
                              22 => "H&#46;M&#46; Gomes"
                              23 => "M&#46;C&#46; Guttierez"
                              24 => "P&#46;M&#46; Hawkey"
                              25 => "P&#46;D&#46; van Helden"
                              26 => "G&#46;V&#46; Kadival"
                              27 => "B&#46;N&#46; Kreiswirth"
                              28 => "K&#46; Kremer"
                              29 => "M&#46; Kubin"
                              30 => "S&#46;P&#46; Kulkarni"
                              31 => "B&#46; Liens"
                              32 => "T&#46; Lillebaek"
                              33 => "M&#46;L&#46; Ho"
                              34 => "C&#46; Martin"
                              35 => "C&#46; Martin"
                              36 => "I&#46; Mokrousov"
                              37 => "O&#46; Narvskaia"
                              38 => "Y&#46;F&#46; Ngeow"
                              39 => "L&#46; Neumann"
                              40 => "S&#46; Niemann"
                              41 => "I&#46; Parwati"
                              42 => "Z&#46; Rahim"
                              43 => "V&#46; Rasolofo-Razanamparany"
                              44 => "T&#46; Rasolonavalona"
                              45 => "M&#46;L&#46; Rossetti"
                              46 => "S&#46; Rusch-Gerdes"
                              47 => "A&#46; Sajduda"
                              48 => "S&#46; Samper"
                              49 => "I&#46;G&#46; Shemyakin"
                              50 => "U&#46;B&#46; Singh"
                              51 => "A&#46; Somoskovi"
                              52 => "R&#46;A&#46; Skuce"
                              53 => "D&#46; van Soolingen"
                              54 => "E&#46;M&#46; Streicher"
                              55 => "P&#46;N&#46; Suffys"
                              56 => "E&#46; Tortoli"
                              57 => "T&#46; Tracevska"
                              58 => "V&#46; Vincent"
                              59 => "T&#46;C&#46; Victor"
                              60 => "R&#46;M&#46; Warren"
                              61 => "S&#46;F&#46; Yap"
                              62 => "K&#46; Zaman"
                              63 => "F&#46; Portaels"
                              64 => "N&#46; Rastogi"
                              65 => "C&#46; Sola"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1186/1471-2180-6-23"
                        "Revista" => array:6 [
                          "tituloSerie" => "BMC Microbiol"
                          "fecha" => "2006"
                          "volumen" => "6"
                          "paginaInicial" => "23"
                          "paginaFinal" => "39"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16519816"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              1 => array:3 [
                "identificador" => "bb0010"
                "etiqueta" => "2&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Evaluation of a Two-Step Approach for Large-Scale&#44; Prospective Genotyping of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> Isolates in the United States"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:7 [
                              0 => "L&#46;S&#46; Cowan"
                              1 => "L&#46; Diem"
                              2 => "T&#46; Monson"
                              3 => "P&#46; Wand"
                              4 => "D&#46; Temporado"
                              5 => "T&#46;V&#46; Oemig"
                              6 => "J&#46;T&#46; Crawford"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1128/JCM.43.2.688-695.2005"
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "2005"
                          "volumen" => "43"
                          "paginaInicial" => "688"
                          "paginaFinal" => "695"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15695665"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              2 => array:3 [
                "identificador" => "bb0015"
                "etiqueta" => "3&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Snapshot of moving and expanding clones of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> and their global distribution assessed by spoligotyping in an international study"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:47 [
                              0 => "I&#46; Filliol"
                              1 => "J&#46;R&#46; Driscoll"
                              2 => "D&#46; van Soolingen"
                              3 => "B&#46;N&#46; Kreiswirth"
                              4 => "K&#46; Kremer"
                              5 => "G&#46; Valetudie"
                              6 => "D&#46;A&#46; Dang"
                              7 => "R&#46; Barlow"
                              8 => "D&#46; Banerjee"
                              9 => "P&#46;J&#46; Bifani"
                              10 => "K&#46; Brudey"
                              11 => "A&#46; Cataldi"
                              12 => "R&#46;C&#46; Cooksey"
                              13 => "D&#46;V&#46; Cousins"
                              14 => "J&#46;W&#46; Dale"
                              15 => "O&#46;A&#46; Dellagostin"
                              16 => "F&#46; Drobniewski"
                              17 => "G&#46; Engelmann"
                              18 => "S&#46; Ferdinand"
                              19 => "D&#46; Gascoyne-Binzi"
                              20 => "M&#46; Gordon"
                              21 => "M&#46;C&#46; Gutierrez"
                              22 => "W&#46;H&#46; Haas"
                              23 => "H&#46; Heersma"
                              24 => "E&#46; Kassa-Kelembho"
                              25 => "M&#46;L&#46; Ho"
                              26 => "A&#46; Makristathis"
                              27 => "C&#46; Mammina"
                              28 => "G&#46; Martin"
                              29 => "P&#46; Mostrom"
                              30 => "I&#46; Mokrousov"
                              31 => "V&#46; Narbonne"
                              32 => "O&#46; Narvskaya"
                              33 => "A&#46; Nastasi"
                              34 => "S&#46;N&#46; Niobe-Eyangoh"
                              35 => "J&#46;W&#46; Pape"
                              36 => "V&#46; Rasolofo-Razanamparany"
                              37 => "M&#46; Ridell"
                              38 => "M&#46;L&#46; Rossetti"
                              39 => "F&#46; Stauffer"
                              40 => "P&#46;N&#46; Suffys"
                              41 => "H&#46; Takiff"
                              42 => "J&#46; Texier-Maugein"
                              43 => "V&#46; Vincent"
                              44 => "J&#46;H&#46; de Waard"
                              45 => "C&#46; Sola"
                              46 => "N&#46; Rastogi"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:7 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "2003"
                          "volumen" => "41"
                          "paginaInicial" => "1963"
                          "paginaFinal" => "1970"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12734235"
                              "web" => "Medline"
                            ]
                          ]
                          "itemHostRev" => array:3 [
                            "pii" => "S0140673601060755"
                            "estado" => "S300"
                            "issn" => "01406736"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              3 => array:3 [
                "identificador" => "bb0020"
                "etiqueta" => "4&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Global distribution of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> spoligotypes"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:49 [
                              0 => "I&#46; Filliol"
                              1 => "J&#46;R&#46; Driscoll"
                              2 => "D&#46; Van Soolingen"
                              3 => "B&#46;N&#46; Kreiswirth"
                              4 => "K&#46; Kremer"
                              5 => "G&#46; Valetudie"
                              6 => "D&#46;D&#46; Anh"
                              7 => "R&#46; Barlow"
                              8 => "D&#46; Banerjee"
                              9 => "P&#46;J&#46; Bifani"
                              10 => "K&#46; Brudey"
                              11 => "A&#46; Cataldi"
                              12 => "R&#46;C&#46; Cooksey"
                              13 => "D&#46;V&#46; Cousins"
                              14 => "J&#46;W&#46; Dale"
                              15 => "O&#46;A&#46; Dellagostin"
                              16 => "F&#46; Drobniewski"
                              17 => "G&#46; Engelmann"
                              18 => "S&#46; Ferdinand"
                              19 => "D&#46; Gascoyne-Binzi"
                              20 => "M&#46; Gordon"
                              21 => "M&#46;C&#46; Gutierrez"
                              22 => "W&#46;H&#46; Haas"
                              23 => "H&#46; Heersma"
                              24 => "G&#46; Kallenius"
                              25 => "E&#46; Kassa-Kelembho"
                              26 => "T&#46; Koivula"
                              27 => "H&#46;M&#46; Ly"
                              28 => "A&#46; Makristathis"
                              29 => "C&#46; Mammina"
                              30 => "G&#46; Martin"
                              31 => "P&#46; Mostrom"
                              32 => "I&#46; Mokrousov"
                              33 => "V&#46; Narbonne"
                              34 => "O&#46; Narvskaya"
                              35 => "A&#46; Nastasi"
                              36 => "S&#46;N&#46; Niobe-Eyangoh"
                              37 => "J&#46;W&#46; Pape"
                              38 => "V&#46; Rasolofo-Razanamparany"
                              39 => "M&#46; Ridell"
                              40 => "M&#46;L&#46; Rossetti"
                              41 => "F&#46; Stauffer"
                              42 => "P&#46;N&#46; Suffys"
                              43 => "H&#46; Takiff"
                              44 => "J&#46; Texier-Maugein"
                              45 => "V&#46; Vincent"
                              46 => "J&#46;H&#46; De Waard"
                              47 => "C&#46; Sola"
                              48 => "N&#46; Rastogi"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.3201/eid0811.020125"
                        "Revista" => array:6 [
                          "tituloSerie" => "Emerg Infect Dis"
                          "fecha" => "2002"
                          "volumen" => "8"
                          "paginaInicial" => "1347"
                          "paginaFinal" => "1349"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12453368"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              4 => array:3 [
                "identificador" => "bb0025"
                "etiqueta" => "5&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Molecular typing of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> based on variable number of tandem DNA repeats used alone and in association with spoligotyping"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:5 [
                              0 => "I&#46; Filliol"
                              1 => "S&#46; Ferdinand"
                              2 => "L&#46; Negroni"
                              3 => "C&#46; Sola"
                              4 => "N&#46; Rastogi"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "2000"
                          "volumen" => "38"
                          "paginaInicial" => "2520"
                          "paginaFinal" => "2524"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10878036"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              5 => array:3 [
                "identificador" => "bb0030"
                "etiqueta" => "6&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Simultaneous detection and strain differentiation of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> for diagnosis and epidemiology"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:11 [
                              0 => "J&#46; Kamerbeek"
                              1 => "L&#46; Schouls"
                              2 => "A&#46; Kolk"
                              3 => "M&#46; van Agterveld"
                              4 => "D&#46; van Soolingen"
                              5 => "S&#46; Kuijper"
                              6 => "A&#46; Bunschoten"
                              7 => "H&#46; Molhuizen"
                              8 => "R&#46; Shaw"
                              9 => "M&#46; Goyal"
                              10 => "J&#46; van Embden"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "1997"
                          "volumen" => "35"
                          "paginaInicial" => "907"
                          "paginaFinal" => "914"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9157152"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              6 => array:3 [
                "identificador" => "bb0035"
                "etiqueta" => "7&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "High&#8211;resolution minisatellite-based typing as a portable approach to global analysis of Mycobacterium tuberculosis molecular epidemiology"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:9 [
                              0 => "E&#46; Mazars"
                              1 => "S&#46; Lesjean"
                              2 => "A&#46;L&#46; Banuls"
                              3 => "M&#46; Gilbert"
                              4 => "V&#46; Vincent"
                              5 => "B&#46; Gicquel"
                              6 => "M&#46; Tibayrenc"
                              7 => "C&#46; Locht"
                              8 => "P&#46; Supply"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1073/pnas.98.4.1901"
                        "Revista" => array:6 [
                          "tituloSerie" => "Proc Natl Acad Sci USA"
                          "fecha" => "2001"
                          "volumen" => "98"
                          "paginaInicial" => "1901"
                          "paginaFinal" => "1906"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11172048"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              7 => array:3 [
                "identificador" => "bb0040"
                "etiqueta" => "8&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Spoligotype database of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>&#58; biogeographic distribution of shared types and epidemiologic and phylogenetic perspectives"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:6 [
                              0 => "C&#46; Sola"
                              1 => "I&#46; Filliol"
                              2 => "M&#46;C&#46; Gutierrez"
                              3 => "I&#46; Mokrousov"
                              4 => "V&#46; Vincent"
                              5 => "N&#46; Rastogi"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.3201/eid0703.010304"
                        "Revista" => array:6 [
                          "tituloSerie" => "Emerg Infect Dis"
                          "fecha" => "2001"
                          "volumen" => "7"
                          "paginaInicial" => "390"
                          "paginaFinal" => "396"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11384514"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              8 => array:3 [
                "identificador" => "bb0045"
                "etiqueta" => "9&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Genotyping of the <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex using MIRUs&#58; association with VNTR and spoligotyping for molecular epidemiology and evolutionary genetics"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:7 [
                              0 => "C&#46; Sola"
                              1 => "I&#46; Filliol"
                              2 => "E&#46; Legrand"
                              3 => "S&#46; Lesjean"
                              4 => "C&#46; Locht"
                              5 => "P&#46; Supply"
                              6 => "N&#46; Rastogi"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "Infect Genet Evol"
                          "fecha" => "2003"
                          "volumen" => "3"
                          "paginaInicial" => "125"
                          "paginaFinal" => "133"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12809807"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              9 => array:3 [
                "identificador" => "bb0050"
                "etiqueta" => "10&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Characterization of ancestral <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> by multiple genetic markers and proposal of genotyping strategy"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:10 [
                              0 => "Y&#46;J&#46; Sun"
                              1 => "A&#46;S&#46; Lee"
                              2 => "S&#46;T&#46; Ng"
                              3 => "S&#46; Ravindran"
                              4 => "K&#46; Kremer"
                              5 => "R&#46; Bellamy"
                              6 => "S&#46;Y&#46; Wong"
                              7 => "D&#46; van Soolingen"
                              8 => "P&#46; Supply"
                              9 => "N&#46;I&#46; Paton"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1128/JCM.42.11.5058-5064.2004"
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "2004"
                          "volumen" => "42"
                          "paginaInicial" => "5058"
                          "paginaFinal" => "5064"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15528696"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              10 => array:3 [
                "identificador" => "bb0055"
                "etiqueta" => "11&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:23 [
                              0 => "P&#46; Supply"
                              1 => "C&#46; Allix"
                              2 => "S&#46; Lesjean"
                              3 => "M&#46; Cardoso-Oelemann"
                              4 => "S&#46; R&#252;sch-Gerdes"
                              5 => "E&#46; Willery"
                              6 => "E&#46; Savine"
                              7 => "P&#46; de Haas"
                              8 => "H&#46; van Deutekom"
                              9 => "S&#46; Roring"
                              10 => "P&#46; Bifani"
                              11 => "N&#46; Kurepina"
                              12 => "B&#46; Kreiswirth"
                              13 => "C&#46; Sola"
                              14 => "N&#46; Rastogi"
                              15 => "V&#46; Vatin"
                              16 => "M&#46;C&#46; Gutierrez"
                              17 => "M&#46; Fauville"
                              18 => "S&#46; Niemann"
                              19 => "R&#46; Skuce"
                              20 => "K&#46; Kremer"
                              21 => "C&#46; Locht"
                              22 => "D&#46; van Soolingen"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1128/JCM.01392-06"
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "2006"
                          "volumen" => "44"
                          "paginaInicial" => "4498"
                          "paginaFinal" => "4510"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17005759"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              11 => array:3 [
                "identificador" => "bb0060"
                "etiqueta" => "12&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Automated high-throughput genotyping for study of global epidemiology of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> based on mycobacterial interspersed repetitive units"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:6 [
                              0 => "P&#46; Supply"
                              1 => "S&#46; Lesjean"
                              2 => "E&#46; Savine"
                              3 => "K&#46; Kremer"
                              4 => "D&#46; van Soolingen"
                              5 => "C&#46; Locht"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1128/JCM.39.10.3563-3571.2001"
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "2001"
                          "volumen" => "39"
                          "paginaInicial" => "3563"
                          "paginaFinal" => "3571"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11574573"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              12 => array:3 [
                "identificador" => "bb0065"
                "etiqueta" => "13&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Strain identification of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> by DNA fingerprinting&#58; recommendations for a standardized methodology"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => true
                            "autores" => array:10 [
                              0 => "J&#46;D&#46; van Embden"
                              1 => "M&#46;D&#46; Cave"
                              2 => "J&#46;T&#46; Crawford"
                              3 => "J&#46;W&#46; Dale"
                              4 => "K&#46;D&#46; Eisenach"
                              5 => "B&#46; Gicquel"
                              6 => "P&#46; Hermans"
                              7 => "C&#46; Martin"
                              8 => "R&#46; McAdam"
                              9 => "T&#46;M&#46; Shinnick"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "1993"
                          "volumen" => "31"
                          "paginaInicial" => "406"
                          "paginaFinal" => "409"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8381814"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              13 => array:3 [
                "identificador" => "bb0070"
                "etiqueta" => "14&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Occurrence and stability of insertion sequences in <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex strains&#58; evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:5 [
                              0 => "D&#46; van Soolingen"
                              1 => "P&#46;W&#46; Hermans"
                              2 => "P&#46;E&#46; de Haas"
                              3 => "D&#46;R&#46; Soll"
                              4 => "J&#46;D&#46; van Embden"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "1991"
                          "volumen" => "29"
                          "paginaInicial" => "2578"
                          "paginaFinal" => "2586"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/1685494"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              14 => array:3 [
                "identificador" => "bb0075"
                "etiqueta" => "15&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Evaluation of strategies for molecular fingerprinting for use in the routine work of a <span class="elsevierStyleItalic">Mycobacterium</span> reference unit"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:3 [
                              0 => "S&#46;M&#46; Wilson"
                              1 => "S&#46; Goss"
                              2 => "F&#46; Drobniewski"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "1998"
                          "volumen" => "36"
                          "paginaInicial" => "3385"
                          "paginaFinal" => "3388"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9774602"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              15 => array:3 [
                "identificador" => "bb0080"
                "etiqueta" => "16&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "A data&#8211;mining approach to spacer oligonucleotide typing of Mycobacterium tuberculosis"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:4 [
                              0 => "M&#46; Sebban"
                              1 => "I&#46; Mokrousov"
                              2 => "N&#46; Rastogi"
                              3 => "C&#46; Sola"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "Bioinformatics"
                          "fecha" => "2002"
                          "volumen" => "18"
                          "paginaInicial" => "235"
                          "paginaFinal" => "243"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11847071"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              16 => array:3 [
                "identificador" => "bb0085"
                "etiqueta" => "17&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Germano de Sousa J&#46; Contribution of spoligotyping to the characterization of the population structure of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> isolates in Portugal"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:5 [
                              0 => "S&#46; David"
                              1 => "D&#46; Raposo Ribeiro"
                              2 => "A&#46; Antunes"
                              3 => "C&#46; Portugal"
                              4 => "L&#46; Sancho"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1016/j.meegid.2007.05.007"
                        "Revista" => array:6 [
                          "tituloSerie" => "Infect Genet Evol"
                          "fecha" => "2007"
                          "volumen" => "7"
                          "paginaInicial" => "609"
                          "paginaFinal" => "617"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17625987"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              17 => array:3 [
                "identificador" => "bb0090"
                "etiqueta" => "18&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Germano de Sousa J&#46; Novos dados sobre o Spoligtyping de estirpes do complexo <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> isoladas no Hospital Fernando Fonseca &#40;Amadora-Sintra&#44; Portugal&#41;"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:7 [
                              0 => "S&#46; David"
                              1 => "V&#46; Barros"
                              2 => "C&#46; Portugal"
                              3 => "A&#46; Antunes"
                              4 => "A&#46; Cardoso"
                              5 => "A&#46; Calado"
                              6 => "L&#46; Sancho"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:5 [
                          "tituloSerie" => "Rev Port Pneumol XI"
                          "fecha" => "2006"
                          "volumen" => "6"
                          "paginaInicial" => "513"
                          "paginaFinal" => "531"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              18 => array:3 [
                "identificador" => "bb0095"
                "etiqueta" => "19&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Germano de Sousa J&#46; Spoligtyping e polimorfismo do gene <span class="elsevierStyleItalic">pncA</span> um cen&#225;rio em duas etapas para o diagn&#243;stico de <span class="elsevierStyleItalic">Mycobacterium bovis</span> em Portugal"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:7 [
                              0 => "S&#46; David"
                              1 => "C&#46; Portugal"
                              2 => "A&#46; Antunes"
                              3 => "A&#46; Calado"
                              4 => "A&#46; Cardoso"
                              5 => "V&#46; Barros"
                              6 => "L&#46; Sancho"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:5 [
                          "tituloSerie" => "Rev Port Pneumol XI"
                          "fecha" => "2006"
                          "volumen" => "6"
                          "paginaInicial" => "533"
                          "paginaFinal" => "556"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              19 => array:3 [
                "identificador" => "bb0100"
                "etiqueta" => "20&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Identifica&#231;&#227;o molecular pelo m&#233;todo de Spoligotyping de estirpes do complexo <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> isoladas no Hospital Fernando Fonseca"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:7 [
                              0 => "S&#46; David"
                              1 => "C&#46; Portugal"
                              2 => "A&#46; Antunes"
                              3 => "A&#46; Cardoso"
                              4 => "A&#46; Calado"
                              5 => "V&#46; Barros"
                              6 => "L&#46; Sancho"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:5 [
                          "tituloSerie" => "Rev Port Pneumol X"
                          "fecha" => "2004"
                          "volumen" => "3"
                          "paginaInicial" => "195"
                          "paginaFinal" => "204"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              20 => array:3 [
                "identificador" => "bb0105"
                "etiqueta" => "21&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Global dissemination of the <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> W-Beijing family strains"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:4 [
                              0 => "P&#46;J&#46; Bifani"
                              1 => "B&#46; Mathema"
                              2 => "N&#46;E&#46; Kurepina"
                              3 => "B&#46;N&#46; Kreiswirth"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "Trends Microbiol"
                          "fecha" => "2002"
                          "volumen" => "10"
                          "paginaInicial" => "45"
                          "paginaFinal" => "52"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11755085"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              21 => array:3 [
                "identificador" => "bb0110"
                "etiqueta" => "22&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Drug-resistant tuberculosis&#44; clinical virulence&#44; and the dominance of the Beijing strain family in Russia"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:8 [
                              0 => "F&#46; Drobniewski"
                              1 => "Y&#46; Balabanova"
                              2 => "V&#46; Nikolayevsky"
                              3 => "M&#46; Ruddy"
                              4 => "S&#46; Kuznetzov"
                              5 => "S&#46; Melentyev"
                              6 => "A&#46; Zakharova"
                              7 => "I&#46; Fedorin"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1001/jama.293.22.2726"
                        "Revista" => array:6 [
                          "tituloSerie" => "JAMA"
                          "fecha" => "2005"
                          "volumen" => "293"
                          "paginaInicial" => "2726"
                          "paginaFinal" => "2731"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15941801"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              22 => array:3 [
                "identificador" => "bb0115"
                "etiqueta" => "23&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Identifying <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex strain families using spoligotypes"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:5 [
                              0 => "I&#46; Vitol"
                              1 => "J&#46; Driscoll"
                              2 => "B&#46; Kreiswirth"
                              3 => "N&#46; Kurepina"
                              4 => "K&#46;P&#46; Bennett"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1016/j.meegid.2006.03.003"
                        "Revista" => array:6 [
                          "tituloSerie" => "Infect Genet Evol"
                          "fecha" => "2006"
                          "volumen" => "6"
                          "paginaInicial" => "491"
                          "paginaFinal" => "504"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16632413"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              23 => array:3 [
                "identificador" => "bb0120"
                "etiqueta" => "24&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Spacer oligonucleotide typing of <span class="elsevierStyleItalic">Mycobacterium bovis</span> strains from cattle and other animals&#58; a tool for studying epidemiology of tuberculosis"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:11 [
                              0 => "A&#46; Aranaz"
                              1 => "E&#46; Li&#233;bana"
                              2 => "A&#46; Mateos"
                              3 => "L&#46; Dominguez"
                              4 => "D&#46; Vidal"
                              5 => "M&#46; Domingo"
                              6 => "O&#46; Gonzolez"
                              7 => "E&#46;F&#46; Rodriguez-Ferri"
                              8 => "A&#46;E&#46; Bunschoten"
                              9 => "J&#46;D&#46;A&#46; Van Embden"
                              10 => "D&#46; Cousins"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "1996"
                          "volumen" => "34"
                          "paginaInicial" => "2734"
                          "paginaFinal" => "2740"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8897175"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              24 => array:3 [
                "identificador" => "bb0125"
                "etiqueta" => "25&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Nature of DNA polymorphism in the direct repeat cluster of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>&#59; Application for strain differentiation by a novel method"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:4 [
                              0 => "P&#46;M&#46;A&#46; Groenen"
                              1 => "A&#46;E&#46; Bunschoten"
                              2 => "D&#46; Van Soolingen"
                              3 => "J&#46;D&#46;A&#46; Van Embden"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "Mol Microbiol"
                          "fecha" => "1993"
                          "volumen" => "10"
                          "paginaInicial" => "1057"
                          "paginaFinal" => "1065"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7934856"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
  ]
  "idiomaDefecto" => "pt"
  "url" => "/08732159/0000001400000004/v1_201509151547/S0873215915302555/v1_201509151547/pt/main.assets"
  "Apartado" => array:4 [
    "identificador" => "43859"
    "tipo" => "SECCION"
    "pt" => array:2 [
      "titulo" => "Do Laborat&#243;rio &#224; Pr&#225;tica Cl&#237;nica"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "pt"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/08732159/0000001400000004/v1_201509151547/S0873215915302555/v1_201509151547/pt/main.pdf?idApp=UINPBA00004E&text.app=https://journalpulmonology.org/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0873215915302555?idApp=UINPBA00004E"
]
Share
Journal Information

Statistics

Follow this link to access the full text of the article

Do Laboratório à Prática Clínica/From Laboratory to Clinical Practice
Utilização estratégica da genotipagem do Mycobacterium tuberculosis no controlo da tuberculose
Strategical use of genotyping of Mycobacterium tuberculosis in tuberculosis control
Susana David1
1 Investigadora Principal, Centro de Tuberculose e Micobactérias, Instituto Nacional de Saúde Dr. Ricardo Jorge, Rua do Campo Alegre 823, 4150-180 Porto
Read
4847
Times
was read the article
2123
Total PDF
2724
Total HTML
Share statistics
 array:24 [
  "pii" => "S0873215915302555"
  "issn" => "08732159"
  "doi" => "10.1016/S0873-2159(15)30255-5"
  "estado" => "S350"
  "fechaPublicacion" => "2008-07-01"
  "aid" => "3"
  "copyright" => "Sociedade Portuguesa de Pneumologia&#47;SPP"
  "copyrightAnyo" => "2008"
  "documento" => "simple-article"
  "crossmark" => 0
  "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
  "subdocumento" => "fla"
  "cita" => "Rev Port Pneumol. 2008;14:509-16"
  "abierto" => array:3 [
    "ES" => true
    "ES2" => true
    "LATM" => true
  ]
  "gratuito" => true
  "lecturas" => array:2 [
    "total" => 2028
    "formatos" => array:3 [
      "EPUB" => 238
      "HTML" => 1133
      "PDF" => 657
    ]
  ]
  "itemSiguiente" => array:19 [
    "pii" => "S0873215915302579"
    "issn" => "08732159"
    "doi" => "10.1016/S0873-2159(15)30257-9"
    "estado" => "S350"
    "fechaPublicacion" => "2008-07-01"
    "aid" => "5"
    "copyright" => "Sociedade Portuguesa de Pneumologia&#47;SPP"
    "documento" => "simple-article"
    "crossmark" => 0
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "fla"
    "cita" => "Rev Port Pneumol. 2008;14:527-34"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 2868
      "formatos" => array:3 [
        "EPUB" => 212
        "HTML" => 1367
        "PDF" => 1289
      ]
    ]
    "pt" => array:11 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Caso Cl&#237;nico&#47;Clinical Case</span>"
      "titulo" => "Revis&#227;o dos tumores da traqueia &#8211; A prop&#243;sito de um caso cl&#237;nico de tumor aden&#243;ide c&#237;stico"
      "tienePdf" => "pt"
      "tieneTextoCompleto" => 0
      "tieneResumen" => array:2 [
        0 => "pt"
        1 => "en"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "527"
          "paginaFinal" => "534"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "en" => array:1 [
          "titulo" => "Tracheal tumors review &#8211; A clinical case of adenoid cystic carcinoma"
        ]
      ]
      "contieneResumen" => array:2 [
        "pt" => true
        "en" => true
      ]
      "contienePdf" => array:1 [
        "pt" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Ant&#243;nio Caiado, Jo&#227;o Moura e S&#225;"
          "autores" => array:2 [
            0 => array:2 [
              "nombre" => "Ant&#243;nio"
              "apellidos" => "Caiado"
            ]
            1 => array:2 [
              "nombre" => "Jo&#227;o"
              "apellidos" => "Moura e S&#225;"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "pt"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0873215915302579?idApp=UINPBA00004E"
    "url" => "/08732159/0000001400000004/v1_201509151547/S0873215915302579/v1_201509151547/pt/main.assets"
  ]
  "itemAnterior" => array:19 [
    "pii" => "S0873215915302567"
    "issn" => "08732159"
    "doi" => "10.1016/S0873-2159(15)30256-7"
    "estado" => "S350"
    "fechaPublicacion" => "2008-07-01"
    "aid" => "4"
    "copyright" => "Sociedade Portuguesa de Pneumologia&#47;SPP"
    "documento" => "simple-article"
    "crossmark" => 0
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "rev"
    "cita" => "Rev Port Pneumol. 2008;14:517-26"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 2932
      "formatos" => array:3 [
        "EPUB" => 231
        "HTML" => 1738
        "PDF" => 963
      ]
    ]
    "pt" => array:11 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Artigo de Revis&#227;o&#47;Revision Article</span>"
      "titulo" => "Tosse em pediatria"
      "tienePdf" => "pt"
      "tieneTextoCompleto" => 0
      "tieneResumen" => array:2 [
        0 => "pt"
        1 => "en"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "517"
          "paginaFinal" => "526"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "en" => array:1 [
          "titulo" => "Cough in paediatrics"
        ]
      ]
      "contieneResumen" => array:2 [
        "pt" => true
        "en" => true
      ]
      "contienePdf" => array:1 [
        "pt" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Susana Martins, Marta Carneiro de Moura, Ana Margarida Neves, Jos&#233; Costa Trindade"
          "autores" => array:4 [
            0 => array:2 [
              "nombre" => "Susana"
              "apellidos" => "Martins"
            ]
            1 => array:2 [
              "nombre" => "Marta Carneiro"
              "apellidos" => "de Moura"
            ]
            2 => array:2 [
              "nombre" => "Ana Margarida"
              "apellidos" => "Neves"
            ]
            3 => array:2 [
              "nombre" => "Jos&#233; Costa"
              "apellidos" => "Trindade"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "pt"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0873215915302567?idApp=UINPBA00004E"
    "url" => "/08732159/0000001400000004/v1_201509151547/S0873215915302567/v1_201509151547/pt/main.assets"
  ]
  "pt" => array:15 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Do Laborat&#243;rio &#224; Pr&#225;tica Cl&#237;nica&#47;From Laboratory to Clinical Practice</span>"
    "titulo" => "Utiliza&#231;&#227;o estrat&#233;gica da genotipagem do <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> no controlo da tuberculose"
    "tieneTextoCompleto" => 0
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "509"
        "paginaFinal" => "516"
      ]
    ]
    "autores" => array:1 [
      0 => array:3 [
        "autoresLista" => "Susana David"
        "autores" => array:1 [
          0 => array:4 [
            "nombre" => "Susana"
            "apellidos" => "David"
            "email" => array:1 [
              0 => "sdavid&#64;ibmc&#46;up&#46;pt"
            ]
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">1</span>"
                "identificador" => "af0005"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:1 [
          0 => array:3 [
            "entidad" => "Investigadora Principal&#44; Centro de Tuberculose e Micobact&#233;rias&#44; Instituto Nacional de Sa&#250;de Dr&#46; Ricardo Jorge&#44; Rua do Campo Alegre 823&#44; 4150-180 Porto"
            "etiqueta" => "1"
            "identificador" => "af0005"
          ]
        ]
      ]
    ]
    "titulosAlternativos" => array:1 [
      "en" => array:1 [
        "titulo" => "<span class="elsevierStyleItalic">Strategical use of genotyping of</span> Mycobacterium tuberculosis <span class="elsevierStyleItalic">in tuberculosis control</span>"
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2008-01-30"
    "fechaAceptado" => "2008-03-26"
    "PalabrasClave" => array:2 [
      "pt" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palavras-chave"
          "identificador" => "xpalclavsec570532"
          "palabras" => array:3 [
            0 => "<span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>"
            1 => "Epidemiologia molecular"
            2 => "genotipagem"
          ]
        ]
      ]
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Key-words"
          "identificador" => "xpalclavsec570533"
          "palabras" => array:3 [
            0 => "<span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>"
            1 => "molecular epidemiology"
            2 => "genotyping"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:2 [
      "pt" => array:2 [
        "titulo" => "Resumo"
        "resumen" => "<span id="as0005" class="elsevierStyleSection elsevierViewall"><p id="sp0005" class="elsevierStyleSimplePara elsevierViewall">A situa&#231;&#227;o da tuberculose em Portugal justifica a aplica&#231;&#227;o de uma estrat&#233;gia de genotipagem do <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>&#44; tanto mais que Portugal encontrase inserido no contexto global de mobilidade das popula&#231;&#245;es humanas e das suas consequ&#234;ncias sobre a pandemia&#46;</p><p id="sp0010" class="elsevierStyleSimplePara elsevierViewall">V&#225;rios estudos internacionais posicionam as t&#233;cnicas do <span class="elsevierStyleItalic">spoligotyping</span> e MIRU-VNTR <span class="elsevierStyleItalic">typing</span> como de primeira linha na epidemiologia molecular do <span class="elsevierStyleItalic">M&#46; tuberculosis</span> por estarem baseadas em tecnologias simples &#40;PCR&#41; e produzirem padr&#245;es&#44; podendo ser traduzidos em c&#243;digo num&#233;rico de interpreta&#231;&#227;o directa&#46; Assim&#44; tem sido proposta a aplica&#231;&#227;o do <span class="elsevierStyleItalic">spoligotyping</span> a todos os isolados cl&#237;nicos&#44; enquanto o MIRU-VNTR <span class="elsevierStyleItalic">typing</span> seria aplicado aos isolados com <span class="elsevierStyleItalic">spoligotype</span> comum&#46; Outras t&#233;cnicas&#44; incluindo o IS6110-RFLP&#44; seriam reservadas a aplica&#231;&#245;es segundo crit&#233;rios seleccionados&#46;</p><p id="sp0015" class="elsevierStyleSimplePara elsevierViewall">Os trabalhos anteriores em Portugal utilizando o <span class="elsevierStyleItalic">spoligotyping</span> apontaram para a vantagem de uma estrat&#233;gia de genotipagem baseada numa amostragem consecutiva e sem crit&#233;rio de pr&#233;-selec&#231;&#227;o&#46; Esta permite a caracteriza&#231;&#227;o da estrutura populacional do <span class="elsevierStyleItalic">M&#46; tuberculosis</span> atrav&#233;s do conhecimento da distribui&#231;&#227;o dos genotipos geograficamente&#44; no tempo e dentro dos v&#225;rios grupos de risco&#46;</p><p id="sp0020" class="elsevierStyleSimplePara elsevierViewall">Por outro lado&#44; a associa&#231;&#227;o do <span class="elsevierStyleItalic">spoligotyping</span> com o MIRU-VNTR <span class="elsevierStyleItalic">typing</span> e&#44; eventualmente&#44; outras t&#233;cnicas&#44; dever&#225; ser avaliada em v&#225;rios contextos&#44; como na identifica&#231;&#227;o de situa&#231;&#245;es de transmiss&#227;o recente&#44; na distin&#231;&#227;o entre epis&#243;dios de reinfec&#231;&#227;o e reca&#237;da&#44; na caracteriza&#231;&#227;o da amplitude e din&#226;mica de transmiss&#227;o da doen&#231;a&#46;</p><p id="sp0025" class="elsevierStyleSimplePara elsevierViewall">A solu&#231;&#227;o do problema da tuberculose em Portugal passa por uma estrutura&#231;&#227;o na utiliza&#231;&#227;o da genotipagem em apoio &#224; luta contra a tuberculose&#44; a ser avaliada atrav&#233;s de exemplos e resultados concretos&#46;</p><p id="sp0030" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleBold">Rev Port Pneumol 2008&#59; XIV &#40;4&#41;&#58; 509-516</span></p></span>"
      ]
      "en" => array:2 [
        "titulo" => "Abstract"
        "resumen" => "<span id="as0010" class="elsevierStyleSection elsevierViewall"><p id="sp0035" class="elsevierStyleSimplePara elsevierViewall">The tuberculosis situation in Portugal justifies the use of a strategy for the genotyping of <span class="elsevierStyleItalic">Mycobacterium tuberculosis&#44;</span> particularly as Portugal is part of the global backdrop of human mobility&#44; something which has a knock-on effect on the pandemic&#46;</p><p id="sp0040" class="elsevierStyleSimplePara elsevierViewall">Several international studies have placed spoligotyping and MIRU-VNTR typing as first line techniques for the molecular epidemiology of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> as these techniques rely on simple technologies &#40;PCR&#41; and produee patterns which are easily translated into a direct interpretation numerical code&#46; Spoligotyping has been accordingly proposed for all the isolates&#44; while MIRU-VNTR typing should be applied to isolates with a common spoliotype&#46; Other techniques&#44; including IS6110-RFLP&#44; should be reserved for use ill accordance with selected criteria&#46;</p><p id="sp0045" class="elsevierStyleSimplePara elsevierViewall">Previous studies in Portugal using spoligotyping have underlined the advantages of a strategy based on sampling consecutive patient isolates with no prior selection criteria&#46; This allows characterisation of the <span class="elsevierStyleItalic">M&#46; tuberculosis</span> population structure through monitoring the distribution of the genotypes geographically over time and within the various risk groups&#46;</p><p id="sp0050" class="elsevierStyleSimplePara elsevierViewall">On the other hand&#44; the association of spoligotyping&#44; MIRU-VNTF &#40;typing and&#44; possibly&#44; other techniques&#44; needs evaluating as part of bigger pictures&#44; including identifying recent transmission situations&#44; distinguishing between reinfection and relapse episodes and ma pping the size and dynamics of disease transmission&#46;</p><p id="sp0055" class="elsevierStyleSimplePara elsevierViewall">The solution to the tuberculosis problem in Portugal implies structuring genotyping&#8217;s role in tuberculosis prevention and control and its evaluation through concrete examples and results&#46;</p><p id="sp0060" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleBold">Rev Port Pneumol 2008&#59; XIV &#40;4&#41;&#58; 509-516</span></p></span>"
      ]
    ]
    "lecturaRecomendada" => array:1 [
      0 => array:3 [
        "vista" => "all"
        "titulo" => "<span class="elsevierStyleSectionTitle" id="st0025">Bibliografia</span>"
        "seccion" => array:1 [
          0 => array:2 [
            "vista" => "all"
            "bibliografiaReferencia" => array:25 [
              0 => array:3 [
                "identificador" => "bb0005"
                "etiqueta" => "1&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "<span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex genetic diversity&#58; mining the fourth international spoligotyping database &#40;SpolDB4&#41; for classification&#44; population genetics and epidemiology"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:66 [
                              0 => "K&#46; Brudey"
                              1 => "J&#46;R&#46; Driscoll"
                              2 => "L&#46; Rigouts"
                              3 => "W&#46;M&#46; Prodinger"
                              4 => "A&#46; Gori"
                              5 => "S&#46;A&#46; Al-Hajoj"
                              6 => "C&#46; Allix"
                              7 => "L&#46; Aristimuno"
                              8 => "J&#46; Arora"
                              9 => "V&#46; Baumanis"
                              10 => "L&#46; Binder"
                              11 => "P&#46; Cafrune"
                              12 => "A&#46; Cataldi"
                              13 => "S&#46; Cheong"
                              14 => "R&#46; Diel"
                              15 => "C&#46; Ellermeier"
                              16 => "J&#46;T&#46; Evans"
                              17 => "M&#46; Fauville-Dufaux"
                              18 => "S&#46; Ferdinand"
                              19 => "Viedma D&#46; Garcia de"
                              20 => "C&#46; Garzelli"
                              21 => "L&#46; Gazzola"
                              22 => "H&#46;M&#46; Gomes"
                              23 => "M&#46;C&#46; Guttierez"
                              24 => "P&#46;M&#46; Hawkey"
                              25 => "P&#46;D&#46; van Helden"
                              26 => "G&#46;V&#46; Kadival"
                              27 => "B&#46;N&#46; Kreiswirth"
                              28 => "K&#46; Kremer"
                              29 => "M&#46; Kubin"
                              30 => "S&#46;P&#46; Kulkarni"
                              31 => "B&#46; Liens"
                              32 => "T&#46; Lillebaek"
                              33 => "M&#46;L&#46; Ho"
                              34 => "C&#46; Martin"
                              35 => "C&#46; Martin"
                              36 => "I&#46; Mokrousov"
                              37 => "O&#46; Narvskaia"
                              38 => "Y&#46;F&#46; Ngeow"
                              39 => "L&#46; Neumann"
                              40 => "S&#46; Niemann"
                              41 => "I&#46; Parwati"
                              42 => "Z&#46; Rahim"
                              43 => "V&#46; Rasolofo-Razanamparany"
                              44 => "T&#46; Rasolonavalona"
                              45 => "M&#46;L&#46; Rossetti"
                              46 => "S&#46; Rusch-Gerdes"
                              47 => "A&#46; Sajduda"
                              48 => "S&#46; Samper"
                              49 => "I&#46;G&#46; Shemyakin"
                              50 => "U&#46;B&#46; Singh"
                              51 => "A&#46; Somoskovi"
                              52 => "R&#46;A&#46; Skuce"
                              53 => "D&#46; van Soolingen"
                              54 => "E&#46;M&#46; Streicher"
                              55 => "P&#46;N&#46; Suffys"
                              56 => "E&#46; Tortoli"
                              57 => "T&#46; Tracevska"
                              58 => "V&#46; Vincent"
                              59 => "T&#46;C&#46; Victor"
                              60 => "R&#46;M&#46; Warren"
                              61 => "S&#46;F&#46; Yap"
                              62 => "K&#46; Zaman"
                              63 => "F&#46; Portaels"
                              64 => "N&#46; Rastogi"
                              65 => "C&#46; Sola"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1186/1471-2180-6-23"
                        "Revista" => array:6 [
                          "tituloSerie" => "BMC Microbiol"
                          "fecha" => "2006"
                          "volumen" => "6"
                          "paginaInicial" => "23"
                          "paginaFinal" => "39"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16519816"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              1 => array:3 [
                "identificador" => "bb0010"
                "etiqueta" => "2&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Evaluation of a Two-Step Approach for Large-Scale&#44; Prospective Genotyping of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> Isolates in the United States"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:7 [
                              0 => "L&#46;S&#46; Cowan"
                              1 => "L&#46; Diem"
                              2 => "T&#46; Monson"
                              3 => "P&#46; Wand"
                              4 => "D&#46; Temporado"
                              5 => "T&#46;V&#46; Oemig"
                              6 => "J&#46;T&#46; Crawford"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1128/JCM.43.2.688-695.2005"
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "2005"
                          "volumen" => "43"
                          "paginaInicial" => "688"
                          "paginaFinal" => "695"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15695665"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              2 => array:3 [
                "identificador" => "bb0015"
                "etiqueta" => "3&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Snapshot of moving and expanding clones of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> and their global distribution assessed by spoligotyping in an international study"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:47 [
                              0 => "I&#46; Filliol"
                              1 => "J&#46;R&#46; Driscoll"
                              2 => "D&#46; van Soolingen"
                              3 => "B&#46;N&#46; Kreiswirth"
                              4 => "K&#46; Kremer"
                              5 => "G&#46; Valetudie"
                              6 => "D&#46;A&#46; Dang"
                              7 => "R&#46; Barlow"
                              8 => "D&#46; Banerjee"
                              9 => "P&#46;J&#46; Bifani"
                              10 => "K&#46; Brudey"
                              11 => "A&#46; Cataldi"
                              12 => "R&#46;C&#46; Cooksey"
                              13 => "D&#46;V&#46; Cousins"
                              14 => "J&#46;W&#46; Dale"
                              15 => "O&#46;A&#46; Dellagostin"
                              16 => "F&#46; Drobniewski"
                              17 => "G&#46; Engelmann"
                              18 => "S&#46; Ferdinand"
                              19 => "D&#46; Gascoyne-Binzi"
                              20 => "M&#46; Gordon"
                              21 => "M&#46;C&#46; Gutierrez"
                              22 => "W&#46;H&#46; Haas"
                              23 => "H&#46; Heersma"
                              24 => "E&#46; Kassa-Kelembho"
                              25 => "M&#46;L&#46; Ho"
                              26 => "A&#46; Makristathis"
                              27 => "C&#46; Mammina"
                              28 => "G&#46; Martin"
                              29 => "P&#46; Mostrom"
                              30 => "I&#46; Mokrousov"
                              31 => "V&#46; Narbonne"
                              32 => "O&#46; Narvskaya"
                              33 => "A&#46; Nastasi"
                              34 => "S&#46;N&#46; Niobe-Eyangoh"
                              35 => "J&#46;W&#46; Pape"
                              36 => "V&#46; Rasolofo-Razanamparany"
                              37 => "M&#46; Ridell"
                              38 => "M&#46;L&#46; Rossetti"
                              39 => "F&#46; Stauffer"
                              40 => "P&#46;N&#46; Suffys"
                              41 => "H&#46; Takiff"
                              42 => "J&#46; Texier-Maugein"
                              43 => "V&#46; Vincent"
                              44 => "J&#46;H&#46; de Waard"
                              45 => "C&#46; Sola"
                              46 => "N&#46; Rastogi"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:7 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "2003"
                          "volumen" => "41"
                          "paginaInicial" => "1963"
                          "paginaFinal" => "1970"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12734235"
                              "web" => "Medline"
                            ]
                          ]
                          "itemHostRev" => array:3 [
                            "pii" => "S0140673601060755"
                            "estado" => "S300"
                            "issn" => "01406736"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              3 => array:3 [
                "identificador" => "bb0020"
                "etiqueta" => "4&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Global distribution of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> spoligotypes"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:49 [
                              0 => "I&#46; Filliol"
                              1 => "J&#46;R&#46; Driscoll"
                              2 => "D&#46; Van Soolingen"
                              3 => "B&#46;N&#46; Kreiswirth"
                              4 => "K&#46; Kremer"
                              5 => "G&#46; Valetudie"
                              6 => "D&#46;D&#46; Anh"
                              7 => "R&#46; Barlow"
                              8 => "D&#46; Banerjee"
                              9 => "P&#46;J&#46; Bifani"
                              10 => "K&#46; Brudey"
                              11 => "A&#46; Cataldi"
                              12 => "R&#46;C&#46; Cooksey"
                              13 => "D&#46;V&#46; Cousins"
                              14 => "J&#46;W&#46; Dale"
                              15 => "O&#46;A&#46; Dellagostin"
                              16 => "F&#46; Drobniewski"
                              17 => "G&#46; Engelmann"
                              18 => "S&#46; Ferdinand"
                              19 => "D&#46; Gascoyne-Binzi"
                              20 => "M&#46; Gordon"
                              21 => "M&#46;C&#46; Gutierrez"
                              22 => "W&#46;H&#46; Haas"
                              23 => "H&#46; Heersma"
                              24 => "G&#46; Kallenius"
                              25 => "E&#46; Kassa-Kelembho"
                              26 => "T&#46; Koivula"
                              27 => "H&#46;M&#46; Ly"
                              28 => "A&#46; Makristathis"
                              29 => "C&#46; Mammina"
                              30 => "G&#46; Martin"
                              31 => "P&#46; Mostrom"
                              32 => "I&#46; Mokrousov"
                              33 => "V&#46; Narbonne"
                              34 => "O&#46; Narvskaya"
                              35 => "A&#46; Nastasi"
                              36 => "S&#46;N&#46; Niobe-Eyangoh"
                              37 => "J&#46;W&#46; Pape"
                              38 => "V&#46; Rasolofo-Razanamparany"
                              39 => "M&#46; Ridell"
                              40 => "M&#46;L&#46; Rossetti"
                              41 => "F&#46; Stauffer"
                              42 => "P&#46;N&#46; Suffys"
                              43 => "H&#46; Takiff"
                              44 => "J&#46; Texier-Maugein"
                              45 => "V&#46; Vincent"
                              46 => "J&#46;H&#46; De Waard"
                              47 => "C&#46; Sola"
                              48 => "N&#46; Rastogi"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.3201/eid0811.020125"
                        "Revista" => array:6 [
                          "tituloSerie" => "Emerg Infect Dis"
                          "fecha" => "2002"
                          "volumen" => "8"
                          "paginaInicial" => "1347"
                          "paginaFinal" => "1349"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12453368"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              4 => array:3 [
                "identificador" => "bb0025"
                "etiqueta" => "5&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Molecular typing of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> based on variable number of tandem DNA repeats used alone and in association with spoligotyping"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:5 [
                              0 => "I&#46; Filliol"
                              1 => "S&#46; Ferdinand"
                              2 => "L&#46; Negroni"
                              3 => "C&#46; Sola"
                              4 => "N&#46; Rastogi"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "2000"
                          "volumen" => "38"
                          "paginaInicial" => "2520"
                          "paginaFinal" => "2524"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10878036"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              5 => array:3 [
                "identificador" => "bb0030"
                "etiqueta" => "6&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Simultaneous detection and strain differentiation of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> for diagnosis and epidemiology"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:11 [
                              0 => "J&#46; Kamerbeek"
                              1 => "L&#46; Schouls"
                              2 => "A&#46; Kolk"
                              3 => "M&#46; van Agterveld"
                              4 => "D&#46; van Soolingen"
                              5 => "S&#46; Kuijper"
                              6 => "A&#46; Bunschoten"
                              7 => "H&#46; Molhuizen"
                              8 => "R&#46; Shaw"
                              9 => "M&#46; Goyal"
                              10 => "J&#46; van Embden"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "1997"
                          "volumen" => "35"
                          "paginaInicial" => "907"
                          "paginaFinal" => "914"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9157152"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              6 => array:3 [
                "identificador" => "bb0035"
                "etiqueta" => "7&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "High&#8211;resolution minisatellite-based typing as a portable approach to global analysis of Mycobacterium tuberculosis molecular epidemiology"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:9 [
                              0 => "E&#46; Mazars"
                              1 => "S&#46; Lesjean"
                              2 => "A&#46;L&#46; Banuls"
                              3 => "M&#46; Gilbert"
                              4 => "V&#46; Vincent"
                              5 => "B&#46; Gicquel"
                              6 => "M&#46; Tibayrenc"
                              7 => "C&#46; Locht"
                              8 => "P&#46; Supply"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1073/pnas.98.4.1901"
                        "Revista" => array:6 [
                          "tituloSerie" => "Proc Natl Acad Sci USA"
                          "fecha" => "2001"
                          "volumen" => "98"
                          "paginaInicial" => "1901"
                          "paginaFinal" => "1906"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11172048"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              7 => array:3 [
                "identificador" => "bb0040"
                "etiqueta" => "8&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Spoligotype database of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>&#58; biogeographic distribution of shared types and epidemiologic and phylogenetic perspectives"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:6 [
                              0 => "C&#46; Sola"
                              1 => "I&#46; Filliol"
                              2 => "M&#46;C&#46; Gutierrez"
                              3 => "I&#46; Mokrousov"
                              4 => "V&#46; Vincent"
                              5 => "N&#46; Rastogi"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.3201/eid0703.010304"
                        "Revista" => array:6 [
                          "tituloSerie" => "Emerg Infect Dis"
                          "fecha" => "2001"
                          "volumen" => "7"
                          "paginaInicial" => "390"
                          "paginaFinal" => "396"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11384514"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              8 => array:3 [
                "identificador" => "bb0045"
                "etiqueta" => "9&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Genotyping of the <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex using MIRUs&#58; association with VNTR and spoligotyping for molecular epidemiology and evolutionary genetics"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:7 [
                              0 => "C&#46; Sola"
                              1 => "I&#46; Filliol"
                              2 => "E&#46; Legrand"
                              3 => "S&#46; Lesjean"
                              4 => "C&#46; Locht"
                              5 => "P&#46; Supply"
                              6 => "N&#46; Rastogi"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "Infect Genet Evol"
                          "fecha" => "2003"
                          "volumen" => "3"
                          "paginaInicial" => "125"
                          "paginaFinal" => "133"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12809807"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              9 => array:3 [
                "identificador" => "bb0050"
                "etiqueta" => "10&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Characterization of ancestral <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> by multiple genetic markers and proposal of genotyping strategy"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:10 [
                              0 => "Y&#46;J&#46; Sun"
                              1 => "A&#46;S&#46; Lee"
                              2 => "S&#46;T&#46; Ng"
                              3 => "S&#46; Ravindran"
                              4 => "K&#46; Kremer"
                              5 => "R&#46; Bellamy"
                              6 => "S&#46;Y&#46; Wong"
                              7 => "D&#46; van Soolingen"
                              8 => "P&#46; Supply"
                              9 => "N&#46;I&#46; Paton"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1128/JCM.42.11.5058-5064.2004"
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "2004"
                          "volumen" => "42"
                          "paginaInicial" => "5058"
                          "paginaFinal" => "5064"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15528696"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              10 => array:3 [
                "identificador" => "bb0055"
                "etiqueta" => "11&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:23 [
                              0 => "P&#46; Supply"
                              1 => "C&#46; Allix"
                              2 => "S&#46; Lesjean"
                              3 => "M&#46; Cardoso-Oelemann"
                              4 => "S&#46; R&#252;sch-Gerdes"
                              5 => "E&#46; Willery"
                              6 => "E&#46; Savine"
                              7 => "P&#46; de Haas"
                              8 => "H&#46; van Deutekom"
                              9 => "S&#46; Roring"
                              10 => "P&#46; Bifani"
                              11 => "N&#46; Kurepina"
                              12 => "B&#46; Kreiswirth"
                              13 => "C&#46; Sola"
                              14 => "N&#46; Rastogi"
                              15 => "V&#46; Vatin"
                              16 => "M&#46;C&#46; Gutierrez"
                              17 => "M&#46; Fauville"
                              18 => "S&#46; Niemann"
                              19 => "R&#46; Skuce"
                              20 => "K&#46; Kremer"
                              21 => "C&#46; Locht"
                              22 => "D&#46; van Soolingen"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1128/JCM.01392-06"
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "2006"
                          "volumen" => "44"
                          "paginaInicial" => "4498"
                          "paginaFinal" => "4510"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17005759"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              11 => array:3 [
                "identificador" => "bb0060"
                "etiqueta" => "12&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Automated high-throughput genotyping for study of global epidemiology of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> based on mycobacterial interspersed repetitive units"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:6 [
                              0 => "P&#46; Supply"
                              1 => "S&#46; Lesjean"
                              2 => "E&#46; Savine"
                              3 => "K&#46; Kremer"
                              4 => "D&#46; van Soolingen"
                              5 => "C&#46; Locht"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1128/JCM.39.10.3563-3571.2001"
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "2001"
                          "volumen" => "39"
                          "paginaInicial" => "3563"
                          "paginaFinal" => "3571"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11574573"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              12 => array:3 [
                "identificador" => "bb0065"
                "etiqueta" => "13&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Strain identification of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> by DNA fingerprinting&#58; recommendations for a standardized methodology"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => true
                            "autores" => array:10 [
                              0 => "J&#46;D&#46; van Embden"
                              1 => "M&#46;D&#46; Cave"
                              2 => "J&#46;T&#46; Crawford"
                              3 => "J&#46;W&#46; Dale"
                              4 => "K&#46;D&#46; Eisenach"
                              5 => "B&#46; Gicquel"
                              6 => "P&#46; Hermans"
                              7 => "C&#46; Martin"
                              8 => "R&#46; McAdam"
                              9 => "T&#46;M&#46; Shinnick"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "1993"
                          "volumen" => "31"
                          "paginaInicial" => "406"
                          "paginaFinal" => "409"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8381814"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              13 => array:3 [
                "identificador" => "bb0070"
                "etiqueta" => "14&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Occurrence and stability of insertion sequences in <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex strains&#58; evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:5 [
                              0 => "D&#46; van Soolingen"
                              1 => "P&#46;W&#46; Hermans"
                              2 => "P&#46;E&#46; de Haas"
                              3 => "D&#46;R&#46; Soll"
                              4 => "J&#46;D&#46; van Embden"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "1991"
                          "volumen" => "29"
                          "paginaInicial" => "2578"
                          "paginaFinal" => "2586"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/1685494"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              14 => array:3 [
                "identificador" => "bb0075"
                "etiqueta" => "15&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Evaluation of strategies for molecular fingerprinting for use in the routine work of a <span class="elsevierStyleItalic">Mycobacterium</span> reference unit"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:3 [
                              0 => "S&#46;M&#46; Wilson"
                              1 => "S&#46; Goss"
                              2 => "F&#46; Drobniewski"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "1998"
                          "volumen" => "36"
                          "paginaInicial" => "3385"
                          "paginaFinal" => "3388"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9774602"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              15 => array:3 [
                "identificador" => "bb0080"
                "etiqueta" => "16&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "A data&#8211;mining approach to spacer oligonucleotide typing of Mycobacterium tuberculosis"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:4 [
                              0 => "M&#46; Sebban"
                              1 => "I&#46; Mokrousov"
                              2 => "N&#46; Rastogi"
                              3 => "C&#46; Sola"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "Bioinformatics"
                          "fecha" => "2002"
                          "volumen" => "18"
                          "paginaInicial" => "235"
                          "paginaFinal" => "243"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11847071"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              16 => array:3 [
                "identificador" => "bb0085"
                "etiqueta" => "17&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Germano de Sousa J&#46; Contribution of spoligotyping to the characterization of the population structure of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> isolates in Portugal"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:5 [
                              0 => "S&#46; David"
                              1 => "D&#46; Raposo Ribeiro"
                              2 => "A&#46; Antunes"
                              3 => "C&#46; Portugal"
                              4 => "L&#46; Sancho"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1016/j.meegid.2007.05.007"
                        "Revista" => array:6 [
                          "tituloSerie" => "Infect Genet Evol"
                          "fecha" => "2007"
                          "volumen" => "7"
                          "paginaInicial" => "609"
                          "paginaFinal" => "617"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17625987"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              17 => array:3 [
                "identificador" => "bb0090"
                "etiqueta" => "18&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Germano de Sousa J&#46; Novos dados sobre o Spoligtyping de estirpes do complexo <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> isoladas no Hospital Fernando Fonseca &#40;Amadora-Sintra&#44; Portugal&#41;"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:7 [
                              0 => "S&#46; David"
                              1 => "V&#46; Barros"
                              2 => "C&#46; Portugal"
                              3 => "A&#46; Antunes"
                              4 => "A&#46; Cardoso"
                              5 => "A&#46; Calado"
                              6 => "L&#46; Sancho"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:5 [
                          "tituloSerie" => "Rev Port Pneumol XI"
                          "fecha" => "2006"
                          "volumen" => "6"
                          "paginaInicial" => "513"
                          "paginaFinal" => "531"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              18 => array:3 [
                "identificador" => "bb0095"
                "etiqueta" => "19&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Germano de Sousa J&#46; Spoligtyping e polimorfismo do gene <span class="elsevierStyleItalic">pncA</span> um cen&#225;rio em duas etapas para o diagn&#243;stico de <span class="elsevierStyleItalic">Mycobacterium bovis</span> em Portugal"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:7 [
                              0 => "S&#46; David"
                              1 => "C&#46; Portugal"
                              2 => "A&#46; Antunes"
                              3 => "A&#46; Calado"
                              4 => "A&#46; Cardoso"
                              5 => "V&#46; Barros"
                              6 => "L&#46; Sancho"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:5 [
                          "tituloSerie" => "Rev Port Pneumol XI"
                          "fecha" => "2006"
                          "volumen" => "6"
                          "paginaInicial" => "533"
                          "paginaFinal" => "556"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              19 => array:3 [
                "identificador" => "bb0100"
                "etiqueta" => "20&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Identifica&#231;&#227;o molecular pelo m&#233;todo de Spoligotyping de estirpes do complexo <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> isoladas no Hospital Fernando Fonseca"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:7 [
                              0 => "S&#46; David"
                              1 => "C&#46; Portugal"
                              2 => "A&#46; Antunes"
                              3 => "A&#46; Cardoso"
                              4 => "A&#46; Calado"
                              5 => "V&#46; Barros"
                              6 => "L&#46; Sancho"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:5 [
                          "tituloSerie" => "Rev Port Pneumol X"
                          "fecha" => "2004"
                          "volumen" => "3"
                          "paginaInicial" => "195"
                          "paginaFinal" => "204"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              20 => array:3 [
                "identificador" => "bb0105"
                "etiqueta" => "21&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Global dissemination of the <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> W-Beijing family strains"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:4 [
                              0 => "P&#46;J&#46; Bifani"
                              1 => "B&#46; Mathema"
                              2 => "N&#46;E&#46; Kurepina"
                              3 => "B&#46;N&#46; Kreiswirth"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "Trends Microbiol"
                          "fecha" => "2002"
                          "volumen" => "10"
                          "paginaInicial" => "45"
                          "paginaFinal" => "52"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11755085"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              21 => array:3 [
                "identificador" => "bb0110"
                "etiqueta" => "22&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Drug-resistant tuberculosis&#44; clinical virulence&#44; and the dominance of the Beijing strain family in Russia"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:8 [
                              0 => "F&#46; Drobniewski"
                              1 => "Y&#46; Balabanova"
                              2 => "V&#46; Nikolayevsky"
                              3 => "M&#46; Ruddy"
                              4 => "S&#46; Kuznetzov"
                              5 => "S&#46; Melentyev"
                              6 => "A&#46; Zakharova"
                              7 => "I&#46; Fedorin"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1001/jama.293.22.2726"
                        "Revista" => array:6 [
                          "tituloSerie" => "JAMA"
                          "fecha" => "2005"
                          "volumen" => "293"
                          "paginaInicial" => "2726"
                          "paginaFinal" => "2731"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15941801"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              22 => array:3 [
                "identificador" => "bb0115"
                "etiqueta" => "23&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Identifying <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex strain families using spoligotypes"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:5 [
                              0 => "I&#46; Vitol"
                              1 => "J&#46; Driscoll"
                              2 => "B&#46; Kreiswirth"
                              3 => "N&#46; Kurepina"
                              4 => "K&#46;P&#46; Bennett"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:2 [
                        "doi" => "10.1016/j.meegid.2006.03.003"
                        "Revista" => array:6 [
                          "tituloSerie" => "Infect Genet Evol"
                          "fecha" => "2006"
                          "volumen" => "6"
                          "paginaInicial" => "491"
                          "paginaFinal" => "504"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16632413"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              23 => array:3 [
                "identificador" => "bb0120"
                "etiqueta" => "24&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Spacer oligonucleotide typing of <span class="elsevierStyleItalic">Mycobacterium bovis</span> strains from cattle and other animals&#58; a tool for studying epidemiology of tuberculosis"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:11 [
                              0 => "A&#46; Aranaz"
                              1 => "E&#46; Li&#233;bana"
                              2 => "A&#46; Mateos"
                              3 => "L&#46; Dominguez"
                              4 => "D&#46; Vidal"
                              5 => "M&#46; Domingo"
                              6 => "O&#46; Gonzolez"
                              7 => "E&#46;F&#46; Rodriguez-Ferri"
                              8 => "A&#46;E&#46; Bunschoten"
                              9 => "J&#46;D&#46;A&#46; Van Embden"
                              10 => "D&#46; Cousins"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "J Clin Microbiol"
                          "fecha" => "1996"
                          "volumen" => "34"
                          "paginaInicial" => "2734"
                          "paginaFinal" => "2740"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8897175"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
              24 => array:3 [
                "identificador" => "bb0125"
                "etiqueta" => "25&#46;"
                "referencia" => array:1 [
                  0 => array:2 [
                    "contribucion" => array:1 [
                      0 => array:2 [
                        "titulo" => "Nature of DNA polymorphism in the direct repeat cluster of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>&#59; Application for strain differentiation by a novel method"
                        "autores" => array:1 [
                          0 => array:2 [
                            "etal" => false
                            "autores" => array:4 [
                              0 => "P&#46;M&#46;A&#46; Groenen"
                              1 => "A&#46;E&#46; Bunschoten"
                              2 => "D&#46; Van Soolingen"
                              3 => "J&#46;D&#46;A&#46; Van Embden"
                            ]
                          ]
                        ]
                      ]
                    ]
                    "host" => array:1 [
                      0 => array:1 [
                        "Revista" => array:6 [
                          "tituloSerie" => "Mol Microbiol"
                          "fecha" => "1993"
                          "volumen" => "10"
                          "paginaInicial" => "1057"
                          "paginaFinal" => "1065"
                          "link" => array:1 [
                            0 => array:2 [
                              "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7934856"
                              "web" => "Medline"
                            ]
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
  ]
  "idiomaDefecto" => "pt"
  "url" => "/08732159/0000001400000004/v1_201509151547/S0873215915302555/v1_201509151547/pt/main.assets"
  "Apartado" => array:4 [
    "identificador" => "43859"
    "tipo" => "SECCION"
    "pt" => array:2 [
      "titulo" => "Do Laborat&#243;rio &#224; Pr&#225;tica Cl&#237;nica"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "pt"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/08732159/0000001400000004/v1_201509151547/S0873215915302555/v1_201509151547/pt/main.pdf?idApp=UINPBA00004E&text.app=https://journalpulmonology.org/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0873215915302555?idApp=UINPBA00004E"
]
Article information
ISSN: 08732159
Original language: Portuguese
The statistics are updated each day
Year/Month Html Pdf Total
2024 November 6 3 9
2024 October 31 32 63
2024 September 29 32 61
2024 August 50 42 92
2024 July 33 35 68
2024 June 20 20 40
2024 May 27 26 53
2024 April 30 30 60
2024 March 31 29 60
2024 February 31 27 58
2024 January 19 30 49
2023 December 15 24 39
2023 November 18 20 38
2023 October 10 26 36
2023 September 21 25 46
2023 August 22 20 42
2023 July 28 33 61
2023 June 19 13 32
2023 May 28 26 54
2023 April 25 20 45
2023 March 28 18 46
2023 February 33 19 52
2023 January 28 18 46
2022 December 15 27 42
2022 November 42 33 75
2022 October 21 26 47
2022 September 17 32 49
2022 August 36 47 83
2022 July 22 30 52
2022 June 15 32 47
2022 May 46 46 92
2022 April 30 39 69
2022 March 32 35 67
2022 February 31 29 60
2022 January 29 47 76
2021 December 19 45 64
2021 November 18 26 44
2021 October 24 36 60
2021 September 25 29 54
2021 August 14 16 30
2021 July 21 23 44
2021 June 12 16 28
2021 May 22 35 57
2021 April 24 36 60
2021 March 22 21 43
2021 February 44 10 54
2021 January 29 15 44
2020 December 11 7 18
2020 November 46 18 64
2020 October 19 18 37
2020 September 44 18 62
2020 August 37 21 58
2020 July 29 20 49
2020 June 50 17 67
2020 May 60 13 73
2020 April 37 20 57
2020 March 45 12 57
2020 February 37 16 53
2020 January 25 19 44
2019 December 52 17 69
2019 November 33 19 52
2019 October 34 18 52
2019 September 42 19 61
2019 August 41 21 62
2019 July 45 25 70
2019 June 36 20 56
2019 May 39 36 75
2019 April 49 28 77
2019 March 44 16 60
2019 February 36 14 50
2019 January 53 16 69
2018 December 15 11 26
2018 November 10 1 11
2018 October 8 8 16
2018 September 13 11 24
2018 August 40 20 60
2018 July 23 22 45
2018 June 45 18 63
2018 May 34 16 50
2018 April 46 28 74
2018 March 29 30 59
2018 February 19 5 24
2018 January 19 11 30
2017 December 22 12 34
2017 November 22 19 41
2017 October 15 16 31
2017 September 16 7 23
2017 August 26 10 36
2017 July 20 6 26
2017 June 19 12 31
2017 May 32 19 51
2017 April 17 30 47
2017 March 12 12 24
2017 February 8 1 9
2017 January 11 2 13
2016 December 10 6 16
2016 November 6 5 11
2016 October 14 7 21
2016 September 1 1 2
2016 August 8 3 11
2016 July 3 2 5
2016 June 0 2 2
2016 May 3 1 4
2016 April 1 1 2
2016 March 1 2 3
2016 February 5 6 11
2016 January 6 5 11
2015 December 5 3 8
2015 November 1 1 2
2015 October 3 4 7
Show all

Follow this link to access the full text of the article

Pulmonology

Are you a health professional able to prescribe or dispense drugs?