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Pyrazinamide susceptibility testing in all isolates of the Mycobacterium tuberculosis complex – a critical analysis
Testes de sensibilidade à pirazinamida em todos os isolados do complexo Mycobacterium tuberculosis – uma análise crítica
J. Cunha
Laboratório de Micobacteriologia do Centro de Diagnóstico Pneumológico do Porto, Administração Regional de Saúde do Norte, Porto, Portugal
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Cunha" "autores" => array:1 [ 0 => array:3 [ "nombre" => "J." "apellidos" => "Cunha" "email" => array:1 [ 0 => "quimcunha@gmail.com" ] ] ] "afiliaciones" => array:1 [ 0 => array:1 [ "entidad" => "Laboratório de Micobacteriologia do Centro de Diagnóstico Pneumológico do Porto, Administração Regional de Saúde do Norte, Porto, Portugal" ] ] ] ] "titulosAlternativos" => array:1 [ "pt" => array:1 [ "titulo" => "Testes de sensibilidade à pirazinamida em todos os isolados do complexo <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> – uma análise crítica" ] ] "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall">In 1950 the World Health Organization (WHO) recognition of the public health threat caused by bovine tuberculosis led governments to implement control measures; these included the pasteurization of milk, rigorous inspection of carcasses in slaughterhouses, the culling of cattle with positive tuberculin tests and prohibiting animals from leaving infected farms. By these measures bovine tuberculosis was controlled or even eradicated. The tuberculosis due to <span class="elsevierStyleItalic">Mycobacterium bovis</span> (<span class="elsevierStyleItalic">M. bovis</span>) is now, therefore, an occupational zoonosis, with the risk groups coming from rural communities, farmers and handlers of cattle, workers of the meat industry, veterinarians and zoological garden workers.<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1,2</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">Nowadays human infection by <span class="elsevierStyleItalic">M. bovis</span> is considered rare in developed countries, accounting for about 1% of all human tuberculosis cases.<a class="elsevierStyleCrossRefs" href="#bib0015"><span class="elsevierStyleSup">3–7</span></a> The laboratory identification of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex (MTC) provides the clinical diagnosis of tuberculosis. The species <span class="elsevierStyleItalic">M. tuberculosis</span> and <span class="elsevierStyleItalic">M. bovis</span>, among others, belong to this complex. Although genotypically very similar, <span class="elsevierStyleItalic">M. bovis</span> is less easily transmitted between humans and is intrinsically resistant to pyrazinamide.<a class="elsevierStyleCrossRefs" href="#bib0020"><span class="elsevierStyleSup">4,8,9</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">Based on this characteristic, the Direção Geral de Saúde (General Directorate of Health, DGS) has recently published Guideline 13/2010, in which it recommends performing drug susceptibility testing (DST) on all first-line anti-tuberculosis drugs (differentiating isoniazid, rifampicin, streptomycin, ethambutol and pyrazinamide) in all isolates of <span class="elsevierStyleItalic">M. tuberculosis</span> complex and further subspecies differentiation when a pyrazinamide resistance isolate is found.<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">10</span></a> It should be noted that the article 9/DT from DGS about first-line DST, in place since the year 2000, does not list the drugs.<a class="elsevierStyleCrossRef" href="#bib0055"><span class="elsevierStyleSup">11</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">This antibiotic, in the context of laboratory diagnosis, is not in the batch of anti-tuberculosis drugs to be first-line tested, although it is commonly used in first-line therapeutic regimens.<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">12</span></a> Testing it requires a different laboratory methodology from other DST, since this drug is only active in an acid environment. In a number of laboratories this test is performed whenever resistance to at least one of the remaining first-line drugs occurs.</p><p id="par0025" class="elsevierStylePara elsevierViewall">The latest international recommendations, from the WHO and from the European Centre for Disease Prevention and Control (ECDC), do not include this drug in the group of the anti-tuberculosis drugs to be routinely tested. Both organizations refer to the low reproducibility and consequently unreliable results. WHO has emphasized also the expense.<a class="elsevierStyleCrossRefs" href="#bib0060"><span class="elsevierStyleSup">12,13</span></a></p><p id="par0030" class="elsevierStylePara elsevierViewall">The unit cost of these tests was calculated based on the selling prices to Administração Regional de Saúde do Norte (Northern Regional Health Administration) of a reagents kit required for DST implementation through a liquid culture system (BACTEC MGIT 960, Becton and Dickinson, Maryland, USA). The pyrazinamide susceptibility test costs around €45, almost three times as much as the other first-line DST. For a total cost evaluation of a test one would need to add the extra time spent, as well as costs of other reagents and laboratory material such as: suspension mediums, ethyl alcohol, bidistilled water, micropipette tips, syringes, needles, etc.</p><p id="par0035" class="elsevierStylePara elsevierViewall">The DGS Guidance, at a national level, will lead to thousands of pyrazinamide susceptibility tests per year, with an increased outflow of hundreds of thousands of Euros, which poses the question whether this can be really justified.</p><p id="par0040" class="elsevierStylePara elsevierViewall">The arguments against, in addition to the international guidelines, also include the fact that studies that have been carried out have shown that the screening of <span class="elsevierStyleItalic">M. bovis</span>, based on pyrazinamide monoresistance, has a low positive predictive value, as well as others that have revealed the existence of <span class="elsevierStyleItalic">M. bovis</span> strains with other anti-tuberculosis resistance patterns.<a class="elsevierStyleCrossRefs" href="#bib0070"><span class="elsevierStyleSup">14,15</span></a></p><p id="par0045" class="elsevierStylePara elsevierViewall">Guideline 13/2010 from DGS is based on the significant increase in bovine tuberculosis; however, it is also clear that, nowadays, the <span class="elsevierStyleItalic">M. bovis</span> infection in human beings is, in practice, restricted to the risk groups. It would be very good if we could count on new prevention strategies, such as better communication between veterinary and human health working groups, to identify patients who are most susceptible to the disease. The pyrazinamide susceptibility test could then be performed on isolates from samples of these specific users and its cost/benefit ratio improved.</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Conflicts of interest</span><p id="par0050" class="elsevierStylePara elsevierViewall">The author has no conflicts of interest to declare.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:2 [ 0 => array:2 [ "identificador" => "sec0005" "titulo" => "Conflicts of interest" ] 1 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "NotaPie" => array:1 [ 0 => array:2 [ "etiqueta" => "☆" "nota" => "<p class="elsevierStyleNotepara">Please cite this article as: Cunha J. Pyrazinamide susceptibility testing in all isolates of the <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex – a critical analysis. Rev Port Pneumol. 2012. <span class="elsevierStyleInterRef" href="doi:10.1016/j.rppneu.2011.12.002">doi:10.1016/j.rppneu.2011.12.002</span>.</p>" ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:15 [ 0 => array:3 [ "identificador" => "bib0005" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Expert Committee on Tuberculosis – report on the fifth edition" "autores" => array:1 [ 0 => array:2 [ "colaboracion" => "World Health Organization" "etal" => false ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "1950" "editorial" => "WHO" "editorialLocalizacion" => "Genebra" ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib0010" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The epidemiology of <span class="elsevierStyleItalic">Mycobacterium bovis</span> infection in animals and man: a review" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "L.M. 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| Year/Month | Html | Total | |
|---|---|---|---|
| 2024 November | 8 | 8 | 16 |
| 2024 October | 31 | 40 | 71 |
| 2024 September | 38 | 35 | 73 |
| 2024 August | 45 | 37 | 82 |
| 2024 July | 35 | 45 | 80 |
| 2024 June | 27 | 31 | 58 |
| 2024 May | 29 | 36 | 65 |
| 2024 April | 25 | 25 | 50 |
| 2024 March | 25 | 32 | 57 |
| 2024 February | 23 | 36 | 59 |
| 2024 January | 13 | 24 | 37 |
| 2023 December | 14 | 33 | 47 |
| 2023 November | 19 | 36 | 55 |
| 2023 October | 16 | 50 | 66 |
| 2023 September | 18 | 27 | 45 |
| 2023 August | 18 | 28 | 46 |
| 2023 July | 15 | 34 | 49 |
| 2023 June | 18 | 22 | 40 |
| 2023 May | 24 | 27 | 51 |
| 2023 April | 12 | 14 | 26 |
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