array:24 [
  "pii" => "S2173511513000171"
  "issn" => "21735115"
  "doi" => "10.1016/j.rppnen.2013.02.006"
  "estado" => "S300"
  "fechaPublicacion" => "2013-03-01"
  "aid" => "119"
  "copyright" => "Sociedade Portuguesa de Pneumologia"
  "copyrightAnyo" => "2012"
  "documento" => "article"
  "crossmark" => 0
  "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
  "subdocumento" => "fla"
  "cita" => "Rev Port Pneumol. 2013;19:70-5"
  "abierto" => array:3 [
    "ES" => true
    "ES2" => true
    "LATM" => true
  ]
  "gratuito" => true
  "lecturas" => array:2 [
    "total" => 5358
    "formatos" => array:3 [
      "EPUB" => 232
      "HTML" => 3714
      "PDF" => 1412
    ]
  ]
  "itemSiguiente" => array:19 [
    "pii" => "S2173511513000183"
    "issn" => "21735115"
    "doi" => "10.1016/j.rppnen.2013.02.007"
    "estado" => "S300"
    "fechaPublicacion" => "2013-03-01"
    "aid" => "127"
    "copyright" => "Sociedade Portuguesa de Pneumologia"
    "documento" => "article"
    "crossmark" => 0
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "sco"
    "cita" => "Rev Port Pneumol. 2013;19:76-9"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 4748
      "formatos" => array:3 [
        "EPUB" => 272
        "HTML" => 3074
        "PDF" => 1402
      ]
    ]
    "en" => array:12 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Case report</span>"
      "titulo" => "<span class="elsevierStyleItalic">Eikenella corrodens</span> and <span class="elsevierStyleItalic">Porphyromonas asaccharolytica</span> pleural empyema in a diabetic patient with obstructive sleep apnea syndrome on noninvasive ventilation"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => array:2 [
        0 => "en"
        1 => "pt"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "76"
          "paginaFinal" => "79"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "pt" => array:1 [
          "titulo" => "Empiema pleural por <span class="elsevierStyleItalic">Eikenella corrodens</span> e <span class="elsevierStyleItalic">Porphyromonas saccharolytica</span> numa doente diab&#233;tica sob ventila&#231;&#227;o n&#227;o invasiva por s&#237;ndrome de apneia obstrutiva do sono"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "pt" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "J&#46; Caiano Gil, R&#46; Calisto, J&#46; Amado, V&#46; Barreto"
          "autores" => array:4 [
            0 => array:2 [
              "nombre" => "J&#46;"
              "apellidos" => "Caiano Gil"
            ]
            1 => array:2 [
              "nombre" => "R&#46;"
              "apellidos" => "Calisto"
            ]
            2 => array:2 [
              "nombre" => "J&#46;"
              "apellidos" => "Amado"
            ]
            3 => array:2 [
              "nombre" => "V&#46;"
              "apellidos" => "Barreto"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511513000183?idApp=UINPBA00004E"
    "url" => "/21735115/0000001900000002/v1_201305151603/S2173511513000183/v1_201305151603/en/main.assets"
  ]
  "itemAnterior" => array:19 [
    "pii" => "S2173511513000213"
    "issn" => "21735115"
    "doi" => "10.1016/j.rppnen.2013.03.002"
    "estado" => "S300"
    "fechaPublicacion" => "2013-03-01"
    "aid" => "122"
    "copyright" => "Sociedade Portuguesa de Pneumologia"
    "documento" => "article"
    "crossmark" => 0
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "fla"
    "cita" => "Rev Port Pneumol. 2013;19:65-9"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 3431
      "formatos" => array:3 [
        "EPUB" => 247
        "HTML" => 2213
        "PDF" => 971
      ]
    ]
    "en" => array:13 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original</span>"
      "titulo" => "Iatrogenic pneumothorax&#58; experience of a Moroccan Emergency Center"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => array:2 [
        0 => "en"
        1 => "pt"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "65"
          "paginaFinal" => "69"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "pt" => array:1 [
          "titulo" => "Pneumot&#243;rax Iatrog&#233;nico&#58; experi&#234;ncia de um Centro de Emerg&#234;ncia Marroquino"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "pt" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "resumenGrafico" => array:2 [
        "original" => 0
        "multimedia" => array:6 [
          "identificador" => "fig0005"
          "etiqueta" => "Figure 1"
          "tipo" => "MULTIMEDIAFIGURA"
          "mostrarFloat" => true
          "mostrarDisplay" => false
          "figura" => array:1 [
            0 => array:4 [
              "imagen" => "gr1.jpeg"
              "Alto" => 897
              "Ancho" => 900
              "Tamanyo" => 123549
            ]
          ]
        ]
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "M&#46;M&#46; El Hammoumi, G&#46; Drissi, A&#46; Achir, A&#46; Benchekroun, A&#46; Benosman"
          "autores" => array:5 [
            0 => array:2 [
              "nombre" => "M&#46;M&#46;"
              "apellidos" => "El Hammoumi"
            ]
            1 => array:2 [
              "nombre" => "G&#46;"
              "apellidos" => "Drissi"
            ]
            2 => array:2 [
              "nombre" => "A&#46;"
              "apellidos" => "Achir"
            ]
            3 => array:2 [
              "nombre" => "A&#46;"
              "apellidos" => "Benchekroun"
            ]
            4 => array:2 [
              "nombre" => "A&#46;"
              "apellidos" => "Benosman"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511513000213?idApp=UINPBA00004E"
    "url" => "/21735115/0000001900000002/v1_201305151603/S2173511513000213/v1_201305151603/en/main.assets"
  ]
  "en" => array:21 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>"
    "titulo" => "Evaluation of the oxidant and antioxidant balance in the pathogenesis of chronic obstructive pulmonary disease"
    "tieneTextoCompleto" => true
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "70"
        "paginaFinal" => "75"
      ]
    ]
    "autores" => array:1 [
      0 => array:4 [
        "autoresLista" => "C&#46; Crist&#243;v&#227;o, L&#46; Crist&#243;v&#227;o, F&#46; Nogueira, M&#46; Bicho"
        "autores" => array:4 [
          0 => array:4 [
            "nombre" => "C&#46;"
            "apellidos" => "Crist&#243;v&#227;o"
            "email" => array:1 [
              0 => "cristina&#46;cristovao&#64;iol&#46;pt"
            ]
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">¿</span>"
                "identificador" => "cor0005"
              ]
            ]
          ]
          1 => array:3 [
            "nombre" => "L&#46;"
            "apellidos" => "Crist&#243;v&#227;o"
            "referencia" => array:4 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff0010"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">c</span>"
                "identificador" => "aff0015"
              ]
              2 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">d</span>"
                "identificador" => "aff0020"
              ]
              3 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">e</span>"
                "identificador" => "aff0025"
              ]
            ]
          ]
          2 => array:3 [
            "nombre" => "F&#46;"
            "apellidos" => "Nogueira"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
            ]
          ]
          3 => array:3 [
            "nombre" => "M&#46;"
            "apellidos" => "Bicho"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">e</span>"
                "identificador" => "aff0025"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:5 [
          0 => array:3 [
            "entidad" => "Servi&#231;o de Pneumologia do Centro Hospitalar de Lisboa Ocidental&#44; Hospital de Egas Moniz&#44; Lisboa&#44; Portugal"
            "etiqueta" => "<span class="elsevierStyleSup">a</span>"
            "identificador" => "aff0005"
          ]
          1 => array:3 [
            "entidad" => "Faculdade de Engenharia e Ci&#234;ncias Naturais&#44; Universidade Lus&#243;fona de Humanidades e Tecnologias de Lisboa&#44; Lisboa&#44; Portugal"
            "etiqueta" => "<span class="elsevierStyleSup">b</span>"
            "identificador" => "aff0010"
          ]
          2 => array:3 [
            "entidad" => "Faculdade de Ci&#234;ncias Biom&#233;dicas&#44; Universidade Lus&#243;fona de Humanidades e Tecnologias de Lisboa&#44; Lisboa&#44; Portugal"
            "etiqueta" => "<span class="elsevierStyleSup">c</span>"
            "identificador" => "aff0015"
          ]
          3 => array:3 [
            "entidad" => "Unidade de Biotecnologia Ambiental&#44; Faculdade de Ci&#234;ncias e Tecnologia&#44; Universidade Nova de Lisboa&#44; Lisboa&#44; Portugal"
            "etiqueta" => "<span class="elsevierStyleSup">d</span>"
            "identificador" => "aff0020"
          ]
          4 => array:3 [
            "entidad" => "Laborat&#243;rio de Gen&#233;tica&#44; Faculdade de Medicina&#44; Universidade de Lisboa&#44; Lisboa&#44; Portugal"
            "etiqueta" => "<span class="elsevierStyleSup">e</span>"
            "identificador" => "aff0025"
          ]
        ]
        "correspondencia" => array:1 [
          0 => array:3 [
            "identificador" => "cor0005"
            "etiqueta" => "&#8270;"
            "correspondencia" => "Corresponding author&#46;"
          ]
        ]
      ]
    ]
    "titulosAlternativos" => array:1 [
      "pt" => array:1 [
        "titulo" => "Avalia&#231;&#227;o do equil&#237;brio entre oxidantes e antioxidantes na patog&#233;nese da doen&#231;a pulmonar obstrutiva cr&#243;nica"
      ]
    ]
    "resumenGrafico" => array:2 [
      "original" => 0
      "multimedia" => array:7 [
        "identificador" => "fig0005"
        "etiqueta" => "Figure 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
            "Alto" => 818
            "Ancho" => 1382
            "Tamanyo" => 60403
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Levels of malondialdehyde &#40;MDA&#41; in plasma from control subjects and COPD patients&#46; Values are expressed as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEM&#46; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001 significantly different between controls and whole COPD group&#46; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01 significantly different between controls and non-smoker patients&#46; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001 significantly different between controls and ex-smoker patients&#46; There was no significant differences between non-smokers and ex-smokers COPD patients&#46;</p>"
        ]
      ]
    ]
    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Chronic obstructive pulmonary disease &#40;COPD&#41; is one of the most common chronic diseases and represents an important cause of morbidity and mortality&#46;<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1&#8211;3</span></a> Experimental studies have provided evidence about an imbalance between oxidants&#47;antioxidants&#44; in favor of reactive oxidizing species &#40;oxidative stress&#41;&#44; associated with COPD&#46;<a class="elsevierStyleCrossRefs" href="#bib0010"><span class="elsevierStyleSup">2&#44;4&#8211;8</span></a> The involvement of oxidative stress in the pathogenesis of COPD appears to be crucial for the manifestation of the inflammatory response of the lung&#46;<a class="elsevierStyleCrossRefs" href="#bib0045"><span class="elsevierStyleSup">9&#8211;13</span></a> The increase of oxidative stress in patients with COPD results from the action of exogenous oxidants &#40;<span class="elsevierStyleItalic">e&#46;g&#46;</span> air pollutants and tobacco components&#41; as well as endogenous oxidants produced during the inflammatory process&#46; However&#44; there are numerous inconsistent results in the studies associated with oxidant and antioxidant imbalance in the pathogenesis of COPD&#46;<a class="elsevierStyleCrossRefs" href="#bib0070"><span class="elsevierStyleSup">14&#44;15</span></a> One of the main targets of oxidative stress are the polyunsaturated fatty acids present in cell membranes&#44;<a class="elsevierStyleCrossRefs" href="#bib0080"><span class="elsevierStyleSup">16&#44;17</span></a> the oxidizing species leading to a multistep process in which a whole is classified as lipid peroxidation&#46; There are several products resulting from lipid peroxidation&#44; among those to consider is a dialdehyde &#40;malonyldialdehyde &#91;MDA&#93;&#41; which has a recognized effect on the level of the human genome and is considered a clastogenic and genotoxic agent&#46;<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">18</span></a> This means that the evaluation of MDA in biological samples can be considered as an indicator of increased lipid peroxidation and&#44; therefore&#44; an indicator of oxidative damage <span class="elsevierStyleItalic">in vivo</span>&#46;<a class="elsevierStyleCrossRefs" href="#bib0080"><span class="elsevierStyleSup">16&#44;19&#44;20</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">There is evidence that oxidative stress reaches the circulation by a fall in the plasma antioxidant capacity &#40;vitamin C&#44; vitamin E&#44; &#946;-carotene and sulphydryls&#41; associated to smoking&#46; In addition&#44; a similar fall in plasma antioxidant occurs in exacerbations of COPD&#46;<a class="elsevierStyleCrossRefs" href="#bib0025"><span class="elsevierStyleSup">5&#44;13</span></a> Epidemiological studies have shown that high dietary intake of antioxidants vitamins C and E related to a lower prevalence of chronic bronchitis in smokers&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">The main objective of this study was to clarify the hypothesis of the existence of an imbalance between oxidant species production and antioxidant defence associated with the pathogenesis of COPD&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Materials and methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Study population</span><p id="par0020" class="elsevierStylePara elsevierViewall">Twenty patients &#40;mean age 71&#46;30<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>7&#46;68 years&#41; with stable COPD &#40;at least more than 6 months without exacerbation history&#41; were studied&#46; Fifteen of them had a past history of smoking and five were non-smokers&#44; with passive smoking exposure or occupational exposure&#46; Clinical and physiological characteristics of COPD group are shown in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#46; The ex-smokers had stopped smoking at least 1 year before their participation in the study&#46; Fifty healthy subjects &#40;mean age 41&#46;60<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>12&#46;31 years&#59; 12 female&#44; 38 male and body mass index&#44; BMI 27&#46;322<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>4&#46;310<span class="elsevierStyleHsp" style=""></span>kg&#47;m<span class="elsevierStyleSup">2</span>&#41; with no history of lung disease were used as control group&#46; Twelve of them were current smokers and thirty-eight had never smoked&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0025" class="elsevierStylePara elsevierViewall">Oxidative stress was assessed in plasma through the determination of the levels of a biomarker such as MDA in COPD patients and in control group&#46; Antioxidant <span class="elsevierStyleItalic">status</span> was evaluated by the quantification of vitamin C and total sulphydryl &#40;<span class="elsevierStyleGlyphsbnd"></span>SH&#41; groups&#44; using spectrophotometric methods&#46;</p><p id="par0030" class="elsevierStylePara elsevierViewall">The study was conducted according to the rules of the declaration of Helsinki&#46; Informed consent was obtained from all subjects participating in the study&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Methods</span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Determination of plasmatic malonyldialdehyde</span><p id="par0035" class="elsevierStylePara elsevierViewall">A biomarker of systemic oxidative stress has been assessed in plasma through the quantification of the levels of a lipid peroxidation derived product&#44; MDA&#46; MDA concentrations were measured spectrophotometrically in terms of thiobarbituric acid reactive substances &#40;TBARS&#41; using a spectrophotometric method modified from Ohkawa et al&#46;<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">22</span></a> The absorbances were read at 532<span class="elsevierStyleHsp" style=""></span>nm corresponding to the colored complex formed between the MDA and thiobarbituric acid &#40;TBA&#41;&#46; The concentration of TBARS was calculated using the MDA concentration and using a calibration curve previously prepared&#46; The concentration of MDA was expressed in nmol&#47;mL of plasma&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Quantification of vitamin C</span><p id="par0040" class="elsevierStylePara elsevierViewall">Vitamin C levels were monitored by the method described by Omaye et al&#46;<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">23</span></a> In brief&#44; the dinitrophenylhydrazine reacts with oxidized vitamin C &#40;oxidized ascorbic acid&#41; to give a colored product&#46; The absorbance was measured at 520<span class="elsevierStyleHsp" style=""></span>nm and is directly proportional to the vitamin C concentration&#46; The levels of vitamin C were expressed in &#956;g&#47;mL of plasma&#46;</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Determination of non-protein sulphydryl groups</span><p id="par0045" class="elsevierStylePara elsevierViewall">The non-protein sulphydryl groups are mainly in the form of reduced glutathione &#40;GSH&#41;&#46; We evaluated the sulphydryl group using a spectrophotometric method involving the use of Ellman&#39;s reagent&#46;<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">24</span></a> The 5&#44;5&#8242;-dithiobis&#40;2-nitrobenzoic acid&#41; &#40;DTNB&#41; undergoes disulfide exchange with sulphydryl groups and 5-thio-2-nitrobenzoate anion &#40;TNB&#41; is formed&#46; The absorbance of the reduced chromogen was measured at 412<span class="elsevierStyleHsp" style=""></span>nm and is directly proportional to the GSH concentration&#46; The levels of sulphydryl groups were expressed in &#956;mol&#47;mL of plasma&#46;</p></span></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Statistical analyses</span><p id="par0050" class="elsevierStylePara elsevierViewall">The results are expressed as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEM of the concentrations of plasma parameters evaluated&#46; The appropriate nonparametric test was chosen for data not normally distributed&#46; Comparisons between two groups were tested using unpaired <span class="elsevierStyleItalic">t</span>-test or Mann&#8211;Whitney <span class="elsevierStyleItalic">U</span>-test&#46; A difference with <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05 was considered statistically significant&#46;</p></span></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Results</span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Oxidative stress and antioxidant <span class="elsevierStyleItalic">status</span></span><p id="par0055" class="elsevierStylePara elsevierViewall">The plasma concentrations of MDA&#44; vitamin C and sulphydryl groups of COPD patients &#40;whole group&#44; non-smoker and ex-smoker patients&#41; and control subjects are shown in <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#46; The marker of oxidative stress &#40;TBARS evaluated as the amount of MDA produced&#41; was found to be significantly &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41; higher in COPD patients when compared with control group&#46; <a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a> shows the MDA plasma levels in controls and COPD patients&#46; We did not find significant differences in MDA levels associated to age but we did observe a significant difference between controls and COPD patients non-smokers &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01&#41; and ex-smokers &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41;&#46; Our results have shown no significant difference in TBARS in ex-smokers COPD patients as compared with non-smokers COPD patients&#46; However&#44; we observed an increase in the marker of oxidative stress associated to the ex-smoker patients&#46;</p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0060" class="elsevierStylePara elsevierViewall">COPD patients had a significant &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41; decrease in antioxidant <span class="elsevierStyleItalic">status</span> &#40;vitamin C and sulphydryl groups&#41; when compared with the control group &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41;&#46; No significant difference was found between non-smokers and ex-smokers in COPD patients&#46; But we observed a decrease in vitamin C associated to the ex-smoker patients&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia></span></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Discussion and conclusions</span><p id="par0065" class="elsevierStylePara elsevierViewall">Oxidants promote lipid peroxidation in cell membrane&#46; The detection of a product of lipid peroxidation in plasma&#44; namely MDA&#44; is a useful tool to show the occurrence of an oxidative stress <span class="elsevierStyleItalic">in vivo</span>&#46; The aim of the present study was to compare the production of MDA and the levels of antioxidants vitamin C and the thiol groups in COPD and healthy controls&#46; Our results show that oxidative stress is an important pathophysiologic change in COPD&#46; The increase in the lipid peroxidation products in plasma of patients with COPD&#44; supports the hypothesis of oxidative stress associated with the disease&#46; According to our results&#44; an increased oxidative stress is most evident in patients with <span class="elsevierStyleItalic">smoking status</span>&#44; but we did not find significant differences in lipid peroxidation and antioxidant <span class="elsevierStyleItalic">status</span> in non-smokers and ex-smokers patients&#46; In addition&#44; we did not observe any significant difference in the marker of oxidative stress and antioxidant <span class="elsevierStyleItalic">status</span> between healthy smokers and non-smokers&#46; Rahman et al&#46;<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">25</span></a> observed that in smokers with and without COPD&#44; an end product of lipid peroxidation is formed in airway epithelial cells&#44; endothelial cells&#44; neutrophils and macrophages&#46; They concluded that oxidative stress resulting from cigarette smoking appears to be more pronounced in those who developed COPD&#46; Previous studies by other authors showed no correlation between TBARs and hydrogen peroxide levels in the COPD associated to cigarette smoking&#46;<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">26</span></a> Although our results should be regarded as preliminary&#44; we observed a tendency for an increase in MDA levels associated to COPD patients with smoking habits&#46;</p><p id="par0070" class="elsevierStylePara elsevierViewall">Antioxidants are also markers of oxidative stress&#46; We can define an antioxidant as any substance that&#44; when present at low concentrations&#44; compared with those of the oxidizable substrate&#44; considerably delays or inhibits oxidation of the substrate&#46;<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">16</span></a> Antioxidants can act at several different stages in an oxidative sequence&#46; Plasma contains a variety of antioxidants&#44; namely vitamin C and thiol groups&#46; The loss of plasma antioxidants may indicate the ongoing biological oxidative stress&#46; When oxidants increase and antioxidant fail&#44; a situation of oxidative stress ensues that leads to excessive molecular damage and tissue injury&#46;<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">16</span></a> Despite some limitations&#44; plasma vitamin C has served as the index for the biochemical evaluation of vitamin C <span class="elsevierStyleItalic">status</span>&#46;<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">23</span></a> Plasmatic vitamin C is particularly important because the gas phase of cigarette smoke induces lipid peroxidation in plasma <span class="elsevierStyleItalic">in vitro</span> that is decreased by vitamin C&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> In addition&#44; as a consequence of the role of oxidative stress in the pathogenesis of emphysema&#44; it has been suggested that dietary antioxidants such as vitamins A&#44; C and E could have a protective effect in smokers&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> In addition&#44; other studies showed decreased total antioxidant capacity in patients with COPD as compared to control subjects&#46;<a class="elsevierStyleCrossRefs" href="#bib0140"><span class="elsevierStyleSup">28&#8211;30</span></a> However discrepant results were found in other studies regarding the relationship between antioxidant <span class="elsevierStyleItalic">status</span> and pulmonary functions in COPD patients&#46;<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">14</span></a> Other studies failed to find a protective effect of antioxidants on the lung function&#46;<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">30</span></a> The molecular mechanisms relating to antioxidant defence in COPD is still lacking&#46;<a class="elsevierStyleCrossRefs" href="#bib0155"><span class="elsevierStyleSup">31&#44;32</span></a> In this study we found that COPD patients had a significant &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41; decrease in antioxidant <span class="elsevierStyleItalic">status</span> &#40;vitamin C and sulphydryl groups&#41; when compared with control group&#46; The significant decrease in vitamin C and sulphydryl groups associated to COPD patients agrees with the hypothesis that glutathione&#44; the most abundant cellular non-protein sulphydryls&#44; and the glutathione redox cycle may be regarded as an antioxidant mechanism involved in the protection against oxidative stress&#46;<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">33</span></a> We did not find a significant difference in the antioxidant <span class="elsevierStyleItalic">status</span> between non-smokers and smokers healthy subjects and between non-smokers and ex-smokers patients but we did observe a tendency toward a decrease in vitamin C associated to COPD patients with smoking habits&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">The difference of age between controls and COPD patients could be a limitation in our study&#46; In order to observe the effect of age&#44; we compared the results in healthy subjects younger than 50 years and those over 50 years&#46; We found no significant difference in plasma MDA levels and antioxidant <span class="elsevierStyleItalic">status</span> associated to age&#46; Our observation is supported by the studies of other authors who found no relationship between plasma antioxidant capacity and protein sulphydryls with age in healthy subjects&#46;<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">21&#44;28</span></a> Other studies reported no age dependent change in the plasma MDA levels in the general population&#46;<a class="elsevierStyleCrossRefs" href="#bib0170"><span class="elsevierStyleSup">34&#44;35</span></a> In addition&#44; other authors have found a marked reduction in MDA associated to chronic airway disorders&#44; particularly COPD&#46; It has been suggested that the increase in MDA levels related to the pathophysiology of COPD may be a promising marker of oxidative stress in chronic pulmonary diseases&#46;<a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">17</span></a></p><p id="par0080" class="elsevierStylePara elsevierViewall">In our study oxygen therapy was not analyzed&#46; However the effect of this therapy in oxidative stress in COPD patients is still controversial&#46; Our results show a significant difference in oxidative stress and antioxidant <span class="elsevierStyleItalic">status</span> between controls and COPD patients &#40;with or without oxygen therapy&#41;&#46; No significant difference was found to be associated with oxygen therapy&#46; Further studies are required to clarify the effects of oxygen therapy in COPD patients&#46;</p><p id="par0085" class="elsevierStylePara elsevierViewall">In conclusion&#44; this study showed that elevated levels of TBARS&#44; namely the end product of oxidant stress appears to be more pronounced in COPD patients&#46; Concomitantly&#44; a significant decrease in the protective antioxidant responses was found to be associated to COPD in both non-smokers and ex-smokers patients&#46; The imbalance between oxidants and antioxidants seems to be an important event associated to the development of COPD&#46; This could provide therapeutic implications in the future&#46;</p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Conflicts of interest</span><p id="par0090" class="elsevierStylePara elsevierViewall">The authors have no conflicts of interest to declare&#46;</p></span></span>"
    "textoCompletoSecciones" => array:1 [
      "secciones" => array:11 [
        0 => array:2 [
          "identificador" => "xres175788"
          "titulo" => "Abstract"
        ]
        1 => array:2 [
          "identificador" => "xpalclavsec164122"
          "titulo" => "Keywords"
        ]
        2 => array:2 [
          "identificador" => "xres175789"
          "titulo" => "Resumo"
        ]
        3 => array:2 [
          "identificador" => "xpalclavsec164123"
          "titulo" => "Palavras-chave"
        ]
        4 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Introduction"
        ]
        5 => array:3 [
          "identificador" => "sec0010"
          "titulo" => "Materials and methods"
          "secciones" => array:3 [
            0 => array:2 [
              "identificador" => "sec0015"
              "titulo" => "Study population"
            ]
            1 => array:3 [
              "identificador" => "sec0020"
              "titulo" => "Methods"
              "secciones" => array:3 [
                0 => array:2 [
                  "identificador" => "sec0025"
                  "titulo" => "Determination of plasmatic malonyldialdehyde"
                ]
                1 => array:2 [
                  "identificador" => "sec0030"
                  "titulo" => "Quantification of vitamin C"
                ]
                2 => array:2 [
                  "identificador" => "sec0035"
                  "titulo" => "Determination of non-protein sulphydryl groups"
                ]
              ]
            ]
            2 => array:2 [
              "identificador" => "sec0040"
              "titulo" => "Statistical analyses"
            ]
          ]
        ]
        6 => array:3 [
          "identificador" => "sec0045"
          "titulo" => "Results"
          "secciones" => array:1 [
            0 => array:2 [
              "identificador" => "sec0050"
              "titulo" => "Oxidative stress and antioxidant status"
            ]
          ]
        ]
        7 => array:2 [
          "identificador" => "sec0055"
          "titulo" => "Discussion and conclusions"
        ]
        8 => array:2 [
          "identificador" => "sec0060"
          "titulo" => "Conflicts of interest"
        ]
        9 => array:2 [
          "identificador" => "xack45053"
          "titulo" => "Acknowledgements"
        ]
        10 => array:1 [
          "titulo" => "References"
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2012-02-20"
    "fechaAceptado" => "2012-09-14"
    "PalabrasClave" => array:2 [
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec164122"
          "palabras" => array:4 [
            0 => "Chronic obstructive pulmonary disease"
            1 => "Oxidative stress"
            2 => "Lipid peroxidation"
            3 => "Antioxidants"
          ]
        ]
      ]
      "pt" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palavras-chave"
          "identificador" => "xpalclavsec164123"
          "palabras" => array:4 [
            0 => "Doen&#231;a pulmonar obstrutiva cr&#243;nica"
            1 => "<span class="elsevierStyleItalic">Stress</span> oxidativo"
            2 => "Peroxida&#231;&#227;o lip&#237;dica"
            3 => "Antioxidantes"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:2 [
      "en" => array:2 [
        "titulo" => "Abstract"
        "resumen" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Chronic obstructive pulmonary disease &#40;COPD&#41; is one of the most common chronic diseases and a major cause of morbidity and mortality&#46; An imbalance between oxidants and antioxidants &#40;oxidative stress&#41; has been proposed as a critical event in the pathogenesis of COPD&#46; The increased oxidative stress in patients with COPD is the result of exogenous oxidants namely pollutants and cigarette smoke as well as endogenous oxidant production during inflammation&#46; The aim of the present study was to clarify the hypothesis about the presence of an imbalance between oxidants and the antioxidant defences associated to COPD&#46; In this study&#44; we evaluated a biomarker of oxidative stress &#40;malondialdehyde&#44; a lipid peroxidation derived product&#41; and non-enzymatic antioxidants &#40;vitamin C and the sulphydryl groups&#41; in COPD patients and healthy controls&#46; The marker of oxidative stress was found to be significantly &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41; higher in COPD patients when compared with control group&#46; No age dependent changes in the plasma levels of lipid peroxidation products were found&#46; COPD patients had a significant &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41; decrease in antioxidant <span class="elsevierStyleItalic">status</span> as compared with control group&#46; Our results show that oxidative stress is an important pathophysiologic change in COPD&#46;</p>"
      ]
      "pt" => array:2 [
        "titulo" => "Resumo"
        "resumen" => "<p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">A doen&#231;a pulmonar obstrutiva cr&#243;nica &#40;DPOC&#41; &#233; uma das doen&#231;as cr&#243;nicas mais comuns e representa uma importante causa de morbilidade e mortalidade&#46; Um desequil&#237;brio entre oxidantes e antioxidantes &#40;<span class="elsevierStyleItalic">stress</span> oxidativo&#41; tem sido proposto como um acontecimento importante na patog&#233;nese da DPOC&#46; O aumento do <span class="elsevierStyleItalic">stress</span> oxidativo em doentes com DPOC &#233; o resultado da presen&#231;a de oxidantes ex&#243;genos&#44; nomeadamente&#44; poluentes e fumo do tabaco&#44; assim como oxidantes end&#243;genos produzidos durante a inflama&#231;&#227;o&#46; O objetivo do presente estudo consistiu em clarificar a hip&#243;tese sobre a exist&#234;ncia de um desequil&#237;brio entre oxidantes e as defesas antioxidantes associado &#224; DPOC&#46; Neste estudo&#44; avaliou-se um biomarcador do <span class="elsevierStyleItalic">stress</span> oxidativo &#40;malonildialde&#237;do&#44; um produto resultante da peroxida&#231;&#227;o lip&#237;dica&#41; e antioxidantes n&#227;o-enzim&#225;ticos &#40;vitamina C e grupos sulfidrilo&#41;&#44; em doentes com DPOC e em controlos saud&#225;veis&#46; Observou-se um aumento significativo &#40;p<span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#44;001&#41; do marcador do <span class="elsevierStyleItalic">stress</span> oxidativo nos doentes com DPOC comparativamente ao grupo controlo&#46; N&#227;o foram encontradas altera&#231;&#245;es dependentes da idade nos n&#237;veis dos produtos da peroxida&#231;&#227;o lip&#237;dica&#46; Os doentes DPOC apresentaram uma diminui&#231;&#227;o significativa &#40;p<span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#44;001&#41; do <span class="elsevierStyleItalic">status</span> antioxidante&#44; comparativamente ao grupo controlo&#46; Os nossos resultados evidenciam que o <span class="elsevierStyleItalic">stress</span> oxidativo representa uma importante altera&#231;&#227;o fisiopatol&#243;gica na DPOC&#46;</p>"
      ]
    ]
    "NotaPie" => array:2 [
      0 => array:2 [
        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara">Please cite this article as&#58; Crist&#243;v&#227;o C&#44; et al&#46; Avalia&#231;&#227;o do equil&#237;brio entre oxidantes e antioxidantes na patog&#233;nese da doen&#231;a pulmonar obstrutiva cr&#243;nica&#46; Rev Port Pneumol&#46; 2013&#46; <span class="elsevierStyleInterRef" href="doi:10.1016/j.rppneu.2012.09.002">http&#58;&#47;&#47;dx&#46;doi&#46;org&#47;10&#46;1016&#47;j&#46;rppneu&#46;2012&#46;09&#46;002</span>&#46;</p>"
      ]
      1 => array:2 [
        "etiqueta" => "&#9734;&#9734;"
        "nota" => "<p class="elsevierStyleNotepara">Department where the study was carried out&#58; This study was conducted in the laboratory of Genetics&#44; Faculdade de Medicina&#44; Universidade de Lisboa and in the laboratories of the Universidade Lus&#243;fona de Humanidades e Tecnologias de Lisboa&#46;</p>"
      ]
    ]
    "multimedia" => array:4 [
      0 => array:7 [
        "identificador" => "fig0005"
        "etiqueta" => "Figure 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
            "Alto" => 818
            "Ancho" => 1382
            "Tamanyo" => 60403
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Levels of malondialdehyde &#40;MDA&#41; in plasma from control subjects and COPD patients&#46; Values are expressed as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEM&#46; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001 significantly different between controls and whole COPD group&#46; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01 significantly different between controls and non-smoker patients&#46; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001 significantly different between controls and ex-smoker patients&#46; There was no significant differences between non-smokers and ex-smokers COPD patients&#46;</p>"
        ]
      ]
      1 => array:7 [
        "identificador" => "fig0010"
        "etiqueta" => "Figure 2"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr2.jpeg"
            "Alto" => 974
            "Ancho" => 2295
            "Tamanyo" => 110836
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Concentration of plasma vitamin C and sulphydryl groups in control subjects and COPD patients&#46; Values are expressed as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEM&#46; COPD patients had a significant &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41; decrease in antioxidant <span class="elsevierStyleItalic">status</span> &#40;vitamin C and <span class="elsevierStyleGlyphsbnd"></span>SH groups&#41; when compared with control group&#46;</p>"
        ]
      ]
      2 => array:7 [
        "identificador" => "tbl0005"
        "etiqueta" => "Table 1"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:1 [
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">Clinical and physiological parameters&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">Values&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Age&#44; years&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">71&#44;30<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>7&#44;68&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Male&#47;female&#44; <span class="elsevierStyleItalic">n</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">11&#47;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Body mass index&#44; kg&#47;m<span class="elsevierStyleSup">2</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">27&#44;918<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#44;724&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Smoking status&#58; ex-smokers&#47;non-smokers&#44; <span class="elsevierStyleItalic">n</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">15&#47;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Packs-years in ex-smokers&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">68&#44;8<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>10&#44;754&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">GOLD stage&#58; I&#47;II&#47;IV&#44; <span class="elsevierStyleItalic">n</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">2&#47;4&#47;14&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">FVC&#44; &#37; predicted&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">77&#44;100<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>5&#44;190&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">FEV1&#44; &#37; predicted&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">58&#44;130<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>5&#44;332&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">FEV1&#47;FVC&#44; &#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">59&#44;453<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>3&#44;805&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">paO2&#44; mmHg&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">66&#44;700<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2&#44;71&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab268168.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Clinical and physiological characteristics of COPD group&#46;</p>"
        ]
      ]
      3 => array:7 [
        "identificador" => "tbl0010"
        "etiqueta" => "Table 2"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">All data are expressed as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEM&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">Controls &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>50&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">Whole COPD group &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>20&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">COPD non-smokers &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>5&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">COPD ex-smokers &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>15&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">MDA &#40;nmol&#47;mL&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">3&#46;830<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;310&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">8&#46;549<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#46;228&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">7&#46;268<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2&#46;309&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">8&#46;976<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#46;472&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">VitaminC &#40;&#956;g&#47;mL&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">6&#46;230<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;220&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">1&#46;866<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;353&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">2&#46;119<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;770&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">1&#46;782<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;408&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Sulphydryl groups &#40;&#956;mol&#47;mL&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;275<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;011&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;135<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;022&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;130<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;021&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;137<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;029&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab268167.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Plasma MDA levels and antioxidant <span class="elsevierStyleItalic">status</span> of the study groups&#46;</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0005"
          "bibliografiaReferencia" => array:35 [
            0 => array:3 [
              "identificador" => "bib0005"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Oxidative stress in chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:3 [
                          "colaboracion" => "Oxidative Stress Study Group"
                          "etal" => false
                          "autores" => array:3 [
                            0 => "J&#46;E&#46; Repine"
                            1 => "A&#46; Bast"
                            2 => "I&#46; Lankhorst"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/ajrccm.156.2.9611013"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "1997"
                        "volumen" => "156"
                        "paginaInicial" => "341"
                        "paginaFinal" => "357"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9279209"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0010"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The pathobiology of chronic bronchitis and emphysema"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "R&#46;M&#46; Tuder"
                            1 => "N&#46;F&#46; Voelkel"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "LibroEditado" => array:4 [
                        "titulo" => "Chronic obstructive lung diseases"
                        "paginaInicial" => "90"
                        "paginaFinal" => "113"
                        "serieFecha" => "2002"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0015"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Epidemiology"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "J&#46; Vestbo"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "LibroEditado" => array:4 [
                        "titulo" => "Chronic obstructive lung diseases"
                        "paginaInicial" => "41"
                        "paginaFinal" => "55"
                        "serieFecha" => "2002"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib0020"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Oxidant&#47;antioxidant imbalance in smokers and chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "I&#46; Rahman"
                            1 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Thorax"
                        "fecha" => "1996"
                        "volumen" => "51"
                        "paginaInicial" => "348"
                        "paginaFinal" => "350"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8733482"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib0025"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Oxidants and antioxidants as therapeutic targets in chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "W&#46; MacNee"
                            1 => "I&#46; Rahman"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/ajrccm.160.supplement_1.15"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "1999"
                        "volumen" => "160"
                        "paginaInicial" => "S58"
                        "paginaFinal" => "S65"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10556172"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib0030"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Oxidants&#47;antioxidants and COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Chest"
                        "fecha" => "2000"
                        "volumen" => "117"
                        "paginaInicial" => "303S"
                        "paginaFinal" => "317S"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10843965"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib0035"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Oxidant&#8211;antioxidant balance in patients with COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "I&#46; Hanta"
                            1 => "A&#46; Kocabas"
                            2 => "N&#46; Canacankatan"
                            3 => "S&#46; Kuleci"
                            4 => "G&#46; Seydaoglu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1007/s00408-005-2561-4"
                      "Revista" => array:6 [
                        "tituloSerie" => "Lung"
                        "fecha" => "2006"
                        "volumen" => "184"
                        "paginaInicial" => "51"
                        "paginaFinal" => "55"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16622773"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib0040"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Impact of oxidative stress on lung diseases"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "H&#46;S&#46; Park"
                            1 => "S&#46;R&#46; Kim"
                            2 => "Y&#46;C&#46; Lee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/j.1440-1843.2008.01447.x"
                      "Revista" => array:6 [
                        "tituloSerie" => "Respirology"
                        "fecha" => "2009"
                        "volumen" => "14"
                        "paginaInicial" => "27"
                        "paginaFinal" => "38"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19144046"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib0045"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "ROS in the local and systemic pathogenesis of COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "R&#46;C&#46;J&#46; Langen"
                            1 => "S&#46;H&#46; Korn"
                            2 => "E&#46;F&#46;M&#46; Wouters"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Free Radic Biol Med"
                        "fecha" => "2003"
                        "volumen" => "35"
                        "paginaInicial" => "226"
                        "paginaFinal" => "235"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12885585"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib0050"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pathogenesis of chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1513/pats.200504-045SR"
                      "Revista" => array:6 [
                        "tituloSerie" => "Proc Am Thorac Soc"
                        "fecha" => "2005"
                        "volumen" => "2"
                        "paginaInicial" => "258"
                        "paginaFinal" => "266"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16267346"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib0055"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Systemic manifestations in chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "V&#46;Z&#46; Dourado"
                            1 => "S&#46;E&#46; Tanni"
                            2 => "S&#46;A&#46; Vale"
                            3 => "M&#46;M&#46; Faganello"
                            4 => "F&#46;F&#46; Sanchez"
                            5 => "I&#46; Godoy"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Bras Pneumol"
                        "fecha" => "2006"
                        "volumen" => "32"
                        "paginaInicial" => "161"
                        "paginaFinal" => "171"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17273586"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            11 => array:3 [
              "identificador" => "bib0060"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pathogenic triad in COPD&#58; oxidative stress&#44; protease&#8211;antiprotease imbalance&#44; and inflammation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "B&#46;M&#46; Fischer"
                            1 => "E&#46; Pavlisko"
                            2 => "J&#46;A&#46; Voynow"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.2147/COPD.S10770"
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Chron Obstruct Pulmon Dis"
                        "fecha" => "2011"
                        "volumen" => "6"
                        "paginaInicial" => "413"
                        "paginaFinal" => "421"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/21857781"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib0065"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pulmonary and systemic oxidant&#47;antioxidant imbalance in chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1513/pats.200411-056SF"
                      "Revista" => array:6 [
                        "tituloSerie" => "Proc Am Thorac Soc"
                        "fecha" => "2005"
                        "volumen" => "2"
                        "paginaInicial" => "50"
                        "paginaFinal" => "60"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16113469"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib0070"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The association between oxidative stress and obstructive lung impairment in patients with COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "Z&#46; Kluchov&#225;"
                            1 => "D&#46; Petr&#225;sova"
                            2 => "P&#46; Joppa"
                            3 => "Z&#46; Dorkov&#225;"
                            4 => "R&#46; Tk&#225;cov&#225;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Phys Res"
                        "fecha" => "2007"
                        "volumen" => "56"
                        "paginaInicial" => "51"
                        "paginaFinal" => "56"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib0075"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pathogenesis of COPD&#46; Part II&#46; Oxidative&#8211;antioxidative imbalance"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "J&#46;C&#46;W&#46; Mak"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2008"
                        "volumen" => "12"
                        "paginaInicial" => "368"
                        "paginaFinal" => "374"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18371260"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            15 => array:3 [
              "identificador" => "bib0080"
              "etiqueta" => "16"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lipid peroxidation and antioxidants as biomarkers of tissue damage"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "J&#46;M&#46;C&#46; Gutteridge"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Clin Chem"
                        "fecha" => "1995"
                        "volumen" => "41"
                        "paginaInicial" => "1819"
                        "paginaFinal" => "1828"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7497639"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            16 => array:3 [
              "identificador" => "bib0085"
              "etiqueta" => "17"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Malondialdehyde in exhaled breath condensate as a marker of oxidative stress in different pulmonary diseases"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "M&#46;L&#46; Bartoli"
                            1 => "F&#46; Novelli"
                            2 => "F&#46; Costa"
                            3 => "L&#46; Malagrin&#242;"
                            4 => "L&#46; Melosini"
                            5 => "E&#46; Bacci"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:4 [
                        "tituloSerie" => "Mediators Inflamm"
                        "fecha" => "2011"
                        "paginaInicial" => "1"
                        "paginaFinal" => "6"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            17 => array:3 [
              "identificador" => "bib0090"
              "etiqueta" => "18"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "DNA damage and oxygen species"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "J&#46; Rueff"
                            1 => "A&#46; Laires"
                            2 => "A&#46; Br&#225;s"
                            3 => "H&#46; Borba"
                            4 => "T&#46; Chaveca"
                            5 => "J&#46; Gaspar"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Libro" => array:4 [
                        "titulo" => "DNA repair mechanisms and their biological implications in mammalian cells"
                        "fecha" => "1989"
                        "editorial" => "Plenum Press"
                        "editorialLocalizacion" => "New York"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            18 => array:3 [
              "identificador" => "bib0095"
              "etiqueta" => "19"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Systemic effects of smoking"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "D&#46;G&#46; Yanbaeva"
                            1 => "M&#46;A&#46; Dentener"
                            2 => "E&#46;C&#46; Creutzberg"
                            3 => "G&#46; Wesselin"
                            4 => "E&#46;F&#46;M&#46; Wouters"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1378/chest.06-2179"
                      "Revista" => array:6 [
                        "tituloSerie" => "Chest"
                        "fecha" => "2007"
                        "volumen" => "131"
                        "paginaInicial" => "1557"
                        "paginaFinal" => "1566"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17494805"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            19 => array:3 [
              "identificador" => "bib0100"
              "etiqueta" => "20"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Blood reflects tissue oxidative stress depending on biomarker and tissue studied"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "A&#46;S&#46; Veskoukis"
                            1 => "M&#46;G&#46; Nikolaidis"
                            2 => "A&#46; Kyparos"
                            3 => "D&#46; Kouretas"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.freeradbiomed.2009.07.014"
                      "Revista" => array:6 [
                        "tituloSerie" => "Free Radic Biol Med"
                        "fecha" => "2009"
                        "volumen" => "47"
                        "paginaInicial" => "1371"
                        "paginaFinal" => "1374"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19616614"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            20 => array:3 [
              "identificador" => "bib0105"
              "etiqueta" => "21"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Is there any relationship between plasma antioxidant capacity and lung function in smokers and in patients with chronic obstructive pulmonary disease&#63;"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "I&#46; Rahman"
                            1 => "E&#46; Swarska"
                            2 => "M&#46; Henry"
                            3 => "J&#46; Stolk"
                            4 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Thorax"
                        "fecha" => "2000"
                        "volumen" => "55"
                        "paginaInicial" => "189"
                        "paginaFinal" => "193"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10679536"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            21 => array:3 [
              "identificador" => "bib0110"
              "etiqueta" => "22"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "H&#46; Ohkawa"
                            1 => "N&#46; Ohishi"
                            2 => "K&#46; Yagi"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Anal Biochem"
                        "fecha" => "1979"
                        "volumen" => "95"
                        "paginaInicial" => "351"
                        "paginaFinal" => "358"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/36810"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            22 => array:3 [
              "identificador" => "bib0115"
              "etiqueta" => "23"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Selected methods for determination of ascorbic acid in animal cells&#44; tissues and fluids"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "S&#46;T&#46; Omaye"
                            1 => "J&#46;D&#46; Turnbull"
                            2 => "H&#46;E&#46; Sauberlich"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "LibroEditado" => array:4 [
                        "titulo" => "Methods enzymol&#44; vitamins and coenzymes"
                        "paginaInicial" => "3"
                        "paginaFinal" => "11"
                        "serieFecha" => "1979"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            23 => array:3 [
              "identificador" => "bib0120"
              "etiqueta" => "24"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Techniques in free radical research"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "C&#46; Rice-Evans"
                            1 => "A&#46; Diplock"
                            2 => "C&#46; Symons"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "LibroEditado" => array:4 [
                        "titulo" => "Laboratory techniques in biochemistry and molecular biology"
                        "paginaInicial" => "227"
                        "paginaFinal" => "229"
                        "serieFecha" => "1991"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            24 => array:3 [
              "identificador" => "bib0125"
              "etiqueta" => "25"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "4-Hydroxy-2-nonenal&#44; a specific lipid peroxidation product&#44; is elevated in lungs of patients with chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "I&#46; Rahman"
                            1 => "A&#46;A&#46;M&#46; van Schadewijk"
                            2 => "A&#46;J&#46;L&#46; Crowther"
                            3 => "P&#46;S&#46; Hiemstra"
                            4 => "J&#46; Stolk"
                            5 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/rccm.2110101"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "2002"
                        "volumen" => "166"
                        "paginaInicial" => "490"
                        "paginaFinal" => "495"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12186826"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            25 => array:3 [
              "identificador" => "bib0130"
              "etiqueta" => "26"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Increased content of thiobarbituric acid-reactive substances and hydrogen peroxide in the expired breath condensate of patients with stable chronic obstructive pulmonary disease&#58; no significant effect of cigarette smoking"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "D&#46; Nowak"
                            1 => "M&#46; Kasielski"
                            2 => "A&#46; Antczak"
                            3 => "T&#46; Pietras"
                            4 => "P&#46; Bialasiewicz"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1053/rmed.1999.0574"
                      "Revista" => array:6 [
                        "tituloSerie" => "Respir Med"
                        "fecha" => "1999"
                        "volumen" => "93"
                        "paginaInicial" => "389"
                        "paginaFinal" => "396"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10464820"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            26 => array:3 [
              "identificador" => "bib0135"
              "etiqueta" => "27"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Cigarette smoke oxidation of human plasma constituents"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "C&#46;E&#46; Cross"
                            1 => "C&#46;A&#46; O&#8217;Neill"
                            2 => "A&#46;Z&#46; Reznick"
                            3 => "M&#46;L&#46; Hu"
                            4 => "L&#46; Marcocci"
                            5 => "L&#46; Packer"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann N Y Acad Sci"
                        "fecha" => "1993"
                        "volumen" => "686"
                        "paginaInicial" => "72"
                        "paginaFinal" => "90"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8512263"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            27 => array:3 [
              "identificador" => "bib0140"
              "etiqueta" => "28"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Systemic oxidative stress in asthma&#44; COPD&#44; and smokers"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "I&#46; Rahman"
                            1 => "D&#46; Morrison"
                            2 => "K&#46; Donaldson"
                            3 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Am J Respir Crit Med"
                        "fecha" => "1996"
                        "volumen" => "154"
                        "paginaInicial" => "1055"
                        "paginaFinal" => "1060"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            28 => array:3 [
              "identificador" => "bib0145"
              "etiqueta" => "29"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The effects of antioxidants on exercise-induced lipid peroxidation in patients with COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "A&#46; Agacdiken"
                            1 => "I&#46; Basyigit"
                            2 => "M&#46; &#214;zden"
                            3 => "F&#46; Yildiz"
                            4 => "D&#46; Ural"
                            5 => "H&#46; Maral"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/j.1440-1843.2003.00526.x"
                      "Revista" => array:6 [
                        "tituloSerie" => "Respirology"
                        "fecha" => "2004"
                        "volumen" => "9"
                        "paginaInicial" => "38"
                        "paginaFinal" => "42"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/14982600"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            29 => array:3 [
              "identificador" => "bib0150"
              "etiqueta" => "30"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Increased oxidative stress and altered levels of antioxidants in chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "A&#46; Nadeem"
                            1 => "H&#46;G&#46; Raj"
                            2 => "S&#46;K&#46; Chhabra"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1007/s10753-006-8965-3"
                      "Revista" => array:6 [
                        "tituloSerie" => "Inflammation"
                        "fecha" => "2005"
                        "volumen" => "29"
                        "paginaInicial" => "23"
                        "paginaFinal" => "32"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16502343"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            30 => array:3 [
              "identificador" => "bib0155"
              "etiqueta" => "31"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The pharmokinetic limitations of antioxidant treatment for COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "R&#46; Foronjy"
                            1 => "A&#46; Wallace"
                            2 => "J&#46; d&#8217;Armiento"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.pupt.2007.10.004"
                      "Revista" => array:6 [
                        "tituloSerie" => "Pulm Pharmacol Ther"
                        "fecha" => "2008"
                        "volumen" => "21"
                        "paginaInicial" => "370"
                        "paginaFinal" => "379"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18054260"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            31 => array:3 [
              "identificador" => "bib0160"
              "etiqueta" => "32"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Antioxidant therapeutic advances in COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "I&#46; Rahman"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1177/1753465808098224"
                      "Revista" => array:6 [
                        "tituloSerie" => "Ther Adv Respir Dis"
                        "fecha" => "2008"
                        "volumen" => "2"
                        "paginaInicial" => "351"
                        "paginaFinal" => "374"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19124382"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            32 => array:3 [
              "identificador" => "bib0165"
              "etiqueta" => "33"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Aging adversely affects the cigarette smoke-induced glutathione adaptive response in the lung"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "N&#46;S&#46; Gould"
                            1 => "E&#46; Min"
                            2 => "S&#46; Gauthier"
                            3 => "H&#46;W&#46; Chu"
                            4 => "R&#46; Martin"
                            5 => "B&#46;J&#46; Day"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/rccm.201003-0442OC"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "2010"
                        "volumen" => "182"
                        "paginaInicial" => "1114"
                        "paginaFinal" => "1122"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20622027"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            33 => array:3 [
              "identificador" => "bib0170"
              "etiqueta" => "34"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Age&#44; sex&#44; cigarette smoking and indices of free radical activity in healthy humans"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "A&#46;B&#46; Bridges"
                            1 => "N&#46;A&#46; Scott"
                            2 => "G&#46;J&#46; Parry"
                            3 => "J&#46;J&#46; Belch"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Eur J Med"
                        "fecha" => "1993"
                        "volumen" => "2"
                        "paginaInicial" => "205"
                        "paginaFinal" => "208"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8261071"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            34 => array:3 [
              "identificador" => "bib0175"
              "etiqueta" => "35"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Factors associated with oxidative stress in human populations"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "G&#46; Block"
                            1 => "M&#46; Dietrich"
                            2 => "E&#46;P&#46; Norkus"
                            3 => "J&#46;D&#46; Morrow"
                            4 => "M&#46; Hudes"
                            5 => "B&#46; Caan"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Epidemiol"
                        "fecha" => "2002"
                        "volumen" => "156"
                        "paginaInicial" => "274"
                        "paginaFinal" => "285"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12142263"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
    "agradecimientos" => array:1 [
      0 => array:3 [
        "identificador" => "xack45053"
        "titulo" => "Acknowledgements"
        "texto" => "<p id="par0095" class="elsevierStylePara elsevierViewall">We thank Professor J&#46; Mexia who greatly contributed to the Statistical Analyses of this work&#46; We extended our appreciation to patients and the healthy volunteers who generously collaborated in this study&#46;</p>"
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/21735115/0000001900000002/v1_201305151603/S2173511513000171/v1_201305151603/en/main.assets"
  "Apartado" => array:4 [
    "identificador" => "9710"
    "tipo" => "SECCION"
    "en" => array:2 [
      "titulo" => "Original articles"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "en"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/21735115/0000001900000002/v1_201305151603/S2173511513000171/v1_201305151603/en/main.pdf?idApp=UINPBA00004E&text.app=https://journalpulmonology.org/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511513000171?idApp=UINPBA00004E"
]
Share
Journal Information

Statistics

Follow this link to access the full text of the article

Original article
Evaluation of the oxidant and antioxidant balance in the pathogenesis of chronic obstructive pulmonary disease
Avaliação do equilíbrio entre oxidantes e antioxidantes na patogénese da doença pulmonar obstrutiva crónica
C. Cristóvãoa,
Corresponding author
cristina.cristovao@iol.pt

Corresponding author.
, L. Cristóvãob,c,d,e, F. Nogueiraa, M. Bichoe
a Serviço de Pneumologia do Centro Hospitalar de Lisboa Ocidental, Hospital de Egas Moniz, Lisboa, Portugal
b Faculdade de Engenharia e Ciências Naturais, Universidade Lusófona de Humanidades e Tecnologias de Lisboa, Lisboa, Portugal
c Faculdade de Ciências Biomédicas, Universidade Lusófona de Humanidades e Tecnologias de Lisboa, Lisboa, Portugal
d Unidade de Biotecnologia Ambiental, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Lisboa, Portugal
e Laboratório de Genética, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal
Read
998
Times
was read the article
493
Total PDF
505
Total HTML
Share statistics
 array:24 [
  "pii" => "S2173511513000171"
  "issn" => "21735115"
  "doi" => "10.1016/j.rppnen.2013.02.006"
  "estado" => "S300"
  "fechaPublicacion" => "2013-03-01"
  "aid" => "119"
  "copyright" => "Sociedade Portuguesa de Pneumologia"
  "copyrightAnyo" => "2012"
  "documento" => "article"
  "crossmark" => 0
  "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
  "subdocumento" => "fla"
  "cita" => "Rev Port Pneumol. 2013;19:70-5"
  "abierto" => array:3 [
    "ES" => true
    "ES2" => true
    "LATM" => true
  ]
  "gratuito" => true
  "lecturas" => array:2 [
    "total" => 5358
    "formatos" => array:3 [
      "EPUB" => 232
      "HTML" => 3714
      "PDF" => 1412
    ]
  ]
  "itemSiguiente" => array:19 [
    "pii" => "S2173511513000183"
    "issn" => "21735115"
    "doi" => "10.1016/j.rppnen.2013.02.007"
    "estado" => "S300"
    "fechaPublicacion" => "2013-03-01"
    "aid" => "127"
    "copyright" => "Sociedade Portuguesa de Pneumologia"
    "documento" => "article"
    "crossmark" => 0
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "sco"
    "cita" => "Rev Port Pneumol. 2013;19:76-9"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 4748
      "formatos" => array:3 [
        "EPUB" => 272
        "HTML" => 3074
        "PDF" => 1402
      ]
    ]
    "en" => array:12 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Case report</span>"
      "titulo" => "<span class="elsevierStyleItalic">Eikenella corrodens</span> and <span class="elsevierStyleItalic">Porphyromonas asaccharolytica</span> pleural empyema in a diabetic patient with obstructive sleep apnea syndrome on noninvasive ventilation"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => array:2 [
        0 => "en"
        1 => "pt"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "76"
          "paginaFinal" => "79"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "pt" => array:1 [
          "titulo" => "Empiema pleural por <span class="elsevierStyleItalic">Eikenella corrodens</span> e <span class="elsevierStyleItalic">Porphyromonas saccharolytica</span> numa doente diab&#233;tica sob ventila&#231;&#227;o n&#227;o invasiva por s&#237;ndrome de apneia obstrutiva do sono"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "pt" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "J&#46; Caiano Gil, R&#46; Calisto, J&#46; Amado, V&#46; Barreto"
          "autores" => array:4 [
            0 => array:2 [
              "nombre" => "J&#46;"
              "apellidos" => "Caiano Gil"
            ]
            1 => array:2 [
              "nombre" => "R&#46;"
              "apellidos" => "Calisto"
            ]
            2 => array:2 [
              "nombre" => "J&#46;"
              "apellidos" => "Amado"
            ]
            3 => array:2 [
              "nombre" => "V&#46;"
              "apellidos" => "Barreto"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511513000183?idApp=UINPBA00004E"
    "url" => "/21735115/0000001900000002/v1_201305151603/S2173511513000183/v1_201305151603/en/main.assets"
  ]
  "itemAnterior" => array:19 [
    "pii" => "S2173511513000213"
    "issn" => "21735115"
    "doi" => "10.1016/j.rppnen.2013.03.002"
    "estado" => "S300"
    "fechaPublicacion" => "2013-03-01"
    "aid" => "122"
    "copyright" => "Sociedade Portuguesa de Pneumologia"
    "documento" => "article"
    "crossmark" => 0
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "fla"
    "cita" => "Rev Port Pneumol. 2013;19:65-9"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 3431
      "formatos" => array:3 [
        "EPUB" => 247
        "HTML" => 2213
        "PDF" => 971
      ]
    ]
    "en" => array:13 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original</span>"
      "titulo" => "Iatrogenic pneumothorax&#58; experience of a Moroccan Emergency Center"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => array:2 [
        0 => "en"
        1 => "pt"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "65"
          "paginaFinal" => "69"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "pt" => array:1 [
          "titulo" => "Pneumot&#243;rax Iatrog&#233;nico&#58; experi&#234;ncia de um Centro de Emerg&#234;ncia Marroquino"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "pt" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "resumenGrafico" => array:2 [
        "original" => 0
        "multimedia" => array:6 [
          "identificador" => "fig0005"
          "etiqueta" => "Figure 1"
          "tipo" => "MULTIMEDIAFIGURA"
          "mostrarFloat" => true
          "mostrarDisplay" => false
          "figura" => array:1 [
            0 => array:4 [
              "imagen" => "gr1.jpeg"
              "Alto" => 897
              "Ancho" => 900
              "Tamanyo" => 123549
            ]
          ]
        ]
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "M&#46;M&#46; El Hammoumi, G&#46; Drissi, A&#46; Achir, A&#46; Benchekroun, A&#46; Benosman"
          "autores" => array:5 [
            0 => array:2 [
              "nombre" => "M&#46;M&#46;"
              "apellidos" => "El Hammoumi"
            ]
            1 => array:2 [
              "nombre" => "G&#46;"
              "apellidos" => "Drissi"
            ]
            2 => array:2 [
              "nombre" => "A&#46;"
              "apellidos" => "Achir"
            ]
            3 => array:2 [
              "nombre" => "A&#46;"
              "apellidos" => "Benchekroun"
            ]
            4 => array:2 [
              "nombre" => "A&#46;"
              "apellidos" => "Benosman"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511513000213?idApp=UINPBA00004E"
    "url" => "/21735115/0000001900000002/v1_201305151603/S2173511513000213/v1_201305151603/en/main.assets"
  ]
  "en" => array:21 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>"
    "titulo" => "Evaluation of the oxidant and antioxidant balance in the pathogenesis of chronic obstructive pulmonary disease"
    "tieneTextoCompleto" => true
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "70"
        "paginaFinal" => "75"
      ]
    ]
    "autores" => array:1 [
      0 => array:4 [
        "autoresLista" => "C&#46; Crist&#243;v&#227;o, L&#46; Crist&#243;v&#227;o, F&#46; Nogueira, M&#46; Bicho"
        "autores" => array:4 [
          0 => array:4 [
            "nombre" => "C&#46;"
            "apellidos" => "Crist&#243;v&#227;o"
            "email" => array:1 [
              0 => "cristina&#46;cristovao&#64;iol&#46;pt"
            ]
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">¿</span>"
                "identificador" => "cor0005"
              ]
            ]
          ]
          1 => array:3 [
            "nombre" => "L&#46;"
            "apellidos" => "Crist&#243;v&#227;o"
            "referencia" => array:4 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff0010"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">c</span>"
                "identificador" => "aff0015"
              ]
              2 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">d</span>"
                "identificador" => "aff0020"
              ]
              3 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">e</span>"
                "identificador" => "aff0025"
              ]
            ]
          ]
          2 => array:3 [
            "nombre" => "F&#46;"
            "apellidos" => "Nogueira"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
            ]
          ]
          3 => array:3 [
            "nombre" => "M&#46;"
            "apellidos" => "Bicho"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">e</span>"
                "identificador" => "aff0025"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:5 [
          0 => array:3 [
            "entidad" => "Servi&#231;o de Pneumologia do Centro Hospitalar de Lisboa Ocidental&#44; Hospital de Egas Moniz&#44; Lisboa&#44; Portugal"
            "etiqueta" => "<span class="elsevierStyleSup">a</span>"
            "identificador" => "aff0005"
          ]
          1 => array:3 [
            "entidad" => "Faculdade de Engenharia e Ci&#234;ncias Naturais&#44; Universidade Lus&#243;fona de Humanidades e Tecnologias de Lisboa&#44; Lisboa&#44; Portugal"
            "etiqueta" => "<span class="elsevierStyleSup">b</span>"
            "identificador" => "aff0010"
          ]
          2 => array:3 [
            "entidad" => "Faculdade de Ci&#234;ncias Biom&#233;dicas&#44; Universidade Lus&#243;fona de Humanidades e Tecnologias de Lisboa&#44; Lisboa&#44; Portugal"
            "etiqueta" => "<span class="elsevierStyleSup">c</span>"
            "identificador" => "aff0015"
          ]
          3 => array:3 [
            "entidad" => "Unidade de Biotecnologia Ambiental&#44; Faculdade de Ci&#234;ncias e Tecnologia&#44; Universidade Nova de Lisboa&#44; Lisboa&#44; Portugal"
            "etiqueta" => "<span class="elsevierStyleSup">d</span>"
            "identificador" => "aff0020"
          ]
          4 => array:3 [
            "entidad" => "Laborat&#243;rio de Gen&#233;tica&#44; Faculdade de Medicina&#44; Universidade de Lisboa&#44; Lisboa&#44; Portugal"
            "etiqueta" => "<span class="elsevierStyleSup">e</span>"
            "identificador" => "aff0025"
          ]
        ]
        "correspondencia" => array:1 [
          0 => array:3 [
            "identificador" => "cor0005"
            "etiqueta" => "&#8270;"
            "correspondencia" => "Corresponding author&#46;"
          ]
        ]
      ]
    ]
    "titulosAlternativos" => array:1 [
      "pt" => array:1 [
        "titulo" => "Avalia&#231;&#227;o do equil&#237;brio entre oxidantes e antioxidantes na patog&#233;nese da doen&#231;a pulmonar obstrutiva cr&#243;nica"
      ]
    ]
    "resumenGrafico" => array:2 [
      "original" => 0
      "multimedia" => array:7 [
        "identificador" => "fig0005"
        "etiqueta" => "Figure 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
            "Alto" => 818
            "Ancho" => 1382
            "Tamanyo" => 60403
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Levels of malondialdehyde &#40;MDA&#41; in plasma from control subjects and COPD patients&#46; Values are expressed as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEM&#46; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001 significantly different between controls and whole COPD group&#46; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01 significantly different between controls and non-smoker patients&#46; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001 significantly different between controls and ex-smoker patients&#46; There was no significant differences between non-smokers and ex-smokers COPD patients&#46;</p>"
        ]
      ]
    ]
    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Chronic obstructive pulmonary disease &#40;COPD&#41; is one of the most common chronic diseases and represents an important cause of morbidity and mortality&#46;<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1&#8211;3</span></a> Experimental studies have provided evidence about an imbalance between oxidants&#47;antioxidants&#44; in favor of reactive oxidizing species &#40;oxidative stress&#41;&#44; associated with COPD&#46;<a class="elsevierStyleCrossRefs" href="#bib0010"><span class="elsevierStyleSup">2&#44;4&#8211;8</span></a> The involvement of oxidative stress in the pathogenesis of COPD appears to be crucial for the manifestation of the inflammatory response of the lung&#46;<a class="elsevierStyleCrossRefs" href="#bib0045"><span class="elsevierStyleSup">9&#8211;13</span></a> The increase of oxidative stress in patients with COPD results from the action of exogenous oxidants &#40;<span class="elsevierStyleItalic">e&#46;g&#46;</span> air pollutants and tobacco components&#41; as well as endogenous oxidants produced during the inflammatory process&#46; However&#44; there are numerous inconsistent results in the studies associated with oxidant and antioxidant imbalance in the pathogenesis of COPD&#46;<a class="elsevierStyleCrossRefs" href="#bib0070"><span class="elsevierStyleSup">14&#44;15</span></a> One of the main targets of oxidative stress are the polyunsaturated fatty acids present in cell membranes&#44;<a class="elsevierStyleCrossRefs" href="#bib0080"><span class="elsevierStyleSup">16&#44;17</span></a> the oxidizing species leading to a multistep process in which a whole is classified as lipid peroxidation&#46; There are several products resulting from lipid peroxidation&#44; among those to consider is a dialdehyde &#40;malonyldialdehyde &#91;MDA&#93;&#41; which has a recognized effect on the level of the human genome and is considered a clastogenic and genotoxic agent&#46;<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">18</span></a> This means that the evaluation of MDA in biological samples can be considered as an indicator of increased lipid peroxidation and&#44; therefore&#44; an indicator of oxidative damage <span class="elsevierStyleItalic">in vivo</span>&#46;<a class="elsevierStyleCrossRefs" href="#bib0080"><span class="elsevierStyleSup">16&#44;19&#44;20</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">There is evidence that oxidative stress reaches the circulation by a fall in the plasma antioxidant capacity &#40;vitamin C&#44; vitamin E&#44; &#946;-carotene and sulphydryls&#41; associated to smoking&#46; In addition&#44; a similar fall in plasma antioxidant occurs in exacerbations of COPD&#46;<a class="elsevierStyleCrossRefs" href="#bib0025"><span class="elsevierStyleSup">5&#44;13</span></a> Epidemiological studies have shown that high dietary intake of antioxidants vitamins C and E related to a lower prevalence of chronic bronchitis in smokers&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">The main objective of this study was to clarify the hypothesis of the existence of an imbalance between oxidant species production and antioxidant defence associated with the pathogenesis of COPD&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Materials and methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Study population</span><p id="par0020" class="elsevierStylePara elsevierViewall">Twenty patients &#40;mean age 71&#46;30<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>7&#46;68 years&#41; with stable COPD &#40;at least more than 6 months without exacerbation history&#41; were studied&#46; Fifteen of them had a past history of smoking and five were non-smokers&#44; with passive smoking exposure or occupational exposure&#46; Clinical and physiological characteristics of COPD group are shown in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#46; The ex-smokers had stopped smoking at least 1 year before their participation in the study&#46; Fifty healthy subjects &#40;mean age 41&#46;60<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>12&#46;31 years&#59; 12 female&#44; 38 male and body mass index&#44; BMI 27&#46;322<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>4&#46;310<span class="elsevierStyleHsp" style=""></span>kg&#47;m<span class="elsevierStyleSup">2</span>&#41; with no history of lung disease were used as control group&#46; Twelve of them were current smokers and thirty-eight had never smoked&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0025" class="elsevierStylePara elsevierViewall">Oxidative stress was assessed in plasma through the determination of the levels of a biomarker such as MDA in COPD patients and in control group&#46; Antioxidant <span class="elsevierStyleItalic">status</span> was evaluated by the quantification of vitamin C and total sulphydryl &#40;<span class="elsevierStyleGlyphsbnd"></span>SH&#41; groups&#44; using spectrophotometric methods&#46;</p><p id="par0030" class="elsevierStylePara elsevierViewall">The study was conducted according to the rules of the declaration of Helsinki&#46; Informed consent was obtained from all subjects participating in the study&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Methods</span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Determination of plasmatic malonyldialdehyde</span><p id="par0035" class="elsevierStylePara elsevierViewall">A biomarker of systemic oxidative stress has been assessed in plasma through the quantification of the levels of a lipid peroxidation derived product&#44; MDA&#46; MDA concentrations were measured spectrophotometrically in terms of thiobarbituric acid reactive substances &#40;TBARS&#41; using a spectrophotometric method modified from Ohkawa et al&#46;<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">22</span></a> The absorbances were read at 532<span class="elsevierStyleHsp" style=""></span>nm corresponding to the colored complex formed between the MDA and thiobarbituric acid &#40;TBA&#41;&#46; The concentration of TBARS was calculated using the MDA concentration and using a calibration curve previously prepared&#46; The concentration of MDA was expressed in nmol&#47;mL of plasma&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Quantification of vitamin C</span><p id="par0040" class="elsevierStylePara elsevierViewall">Vitamin C levels were monitored by the method described by Omaye et al&#46;<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">23</span></a> In brief&#44; the dinitrophenylhydrazine reacts with oxidized vitamin C &#40;oxidized ascorbic acid&#41; to give a colored product&#46; The absorbance was measured at 520<span class="elsevierStyleHsp" style=""></span>nm and is directly proportional to the vitamin C concentration&#46; The levels of vitamin C were expressed in &#956;g&#47;mL of plasma&#46;</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Determination of non-protein sulphydryl groups</span><p id="par0045" class="elsevierStylePara elsevierViewall">The non-protein sulphydryl groups are mainly in the form of reduced glutathione &#40;GSH&#41;&#46; We evaluated the sulphydryl group using a spectrophotometric method involving the use of Ellman&#39;s reagent&#46;<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">24</span></a> The 5&#44;5&#8242;-dithiobis&#40;2-nitrobenzoic acid&#41; &#40;DTNB&#41; undergoes disulfide exchange with sulphydryl groups and 5-thio-2-nitrobenzoate anion &#40;TNB&#41; is formed&#46; The absorbance of the reduced chromogen was measured at 412<span class="elsevierStyleHsp" style=""></span>nm and is directly proportional to the GSH concentration&#46; The levels of sulphydryl groups were expressed in &#956;mol&#47;mL of plasma&#46;</p></span></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Statistical analyses</span><p id="par0050" class="elsevierStylePara elsevierViewall">The results are expressed as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEM of the concentrations of plasma parameters evaluated&#46; The appropriate nonparametric test was chosen for data not normally distributed&#46; Comparisons between two groups were tested using unpaired <span class="elsevierStyleItalic">t</span>-test or Mann&#8211;Whitney <span class="elsevierStyleItalic">U</span>-test&#46; A difference with <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05 was considered statistically significant&#46;</p></span></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Results</span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Oxidative stress and antioxidant <span class="elsevierStyleItalic">status</span></span><p id="par0055" class="elsevierStylePara elsevierViewall">The plasma concentrations of MDA&#44; vitamin C and sulphydryl groups of COPD patients &#40;whole group&#44; non-smoker and ex-smoker patients&#41; and control subjects are shown in <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#46; The marker of oxidative stress &#40;TBARS evaluated as the amount of MDA produced&#41; was found to be significantly &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41; higher in COPD patients when compared with control group&#46; <a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a> shows the MDA plasma levels in controls and COPD patients&#46; We did not find significant differences in MDA levels associated to age but we did observe a significant difference between controls and COPD patients non-smokers &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01&#41; and ex-smokers &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41;&#46; Our results have shown no significant difference in TBARS in ex-smokers COPD patients as compared with non-smokers COPD patients&#46; However&#44; we observed an increase in the marker of oxidative stress associated to the ex-smoker patients&#46;</p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0060" class="elsevierStylePara elsevierViewall">COPD patients had a significant &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41; decrease in antioxidant <span class="elsevierStyleItalic">status</span> &#40;vitamin C and sulphydryl groups&#41; when compared with the control group &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41;&#46; No significant difference was found between non-smokers and ex-smokers in COPD patients&#46; But we observed a decrease in vitamin C associated to the ex-smoker patients&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia></span></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Discussion and conclusions</span><p id="par0065" class="elsevierStylePara elsevierViewall">Oxidants promote lipid peroxidation in cell membrane&#46; The detection of a product of lipid peroxidation in plasma&#44; namely MDA&#44; is a useful tool to show the occurrence of an oxidative stress <span class="elsevierStyleItalic">in vivo</span>&#46; The aim of the present study was to compare the production of MDA and the levels of antioxidants vitamin C and the thiol groups in COPD and healthy controls&#46; Our results show that oxidative stress is an important pathophysiologic change in COPD&#46; The increase in the lipid peroxidation products in plasma of patients with COPD&#44; supports the hypothesis of oxidative stress associated with the disease&#46; According to our results&#44; an increased oxidative stress is most evident in patients with <span class="elsevierStyleItalic">smoking status</span>&#44; but we did not find significant differences in lipid peroxidation and antioxidant <span class="elsevierStyleItalic">status</span> in non-smokers and ex-smokers patients&#46; In addition&#44; we did not observe any significant difference in the marker of oxidative stress and antioxidant <span class="elsevierStyleItalic">status</span> between healthy smokers and non-smokers&#46; Rahman et al&#46;<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">25</span></a> observed that in smokers with and without COPD&#44; an end product of lipid peroxidation is formed in airway epithelial cells&#44; endothelial cells&#44; neutrophils and macrophages&#46; They concluded that oxidative stress resulting from cigarette smoking appears to be more pronounced in those who developed COPD&#46; Previous studies by other authors showed no correlation between TBARs and hydrogen peroxide levels in the COPD associated to cigarette smoking&#46;<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">26</span></a> Although our results should be regarded as preliminary&#44; we observed a tendency for an increase in MDA levels associated to COPD patients with smoking habits&#46;</p><p id="par0070" class="elsevierStylePara elsevierViewall">Antioxidants are also markers of oxidative stress&#46; We can define an antioxidant as any substance that&#44; when present at low concentrations&#44; compared with those of the oxidizable substrate&#44; considerably delays or inhibits oxidation of the substrate&#46;<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">16</span></a> Antioxidants can act at several different stages in an oxidative sequence&#46; Plasma contains a variety of antioxidants&#44; namely vitamin C and thiol groups&#46; The loss of plasma antioxidants may indicate the ongoing biological oxidative stress&#46; When oxidants increase and antioxidant fail&#44; a situation of oxidative stress ensues that leads to excessive molecular damage and tissue injury&#46;<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">16</span></a> Despite some limitations&#44; plasma vitamin C has served as the index for the biochemical evaluation of vitamin C <span class="elsevierStyleItalic">status</span>&#46;<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">23</span></a> Plasmatic vitamin C is particularly important because the gas phase of cigarette smoke induces lipid peroxidation in plasma <span class="elsevierStyleItalic">in vitro</span> that is decreased by vitamin C&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> In addition&#44; as a consequence of the role of oxidative stress in the pathogenesis of emphysema&#44; it has been suggested that dietary antioxidants such as vitamins A&#44; C and E could have a protective effect in smokers&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> In addition&#44; other studies showed decreased total antioxidant capacity in patients with COPD as compared to control subjects&#46;<a class="elsevierStyleCrossRefs" href="#bib0140"><span class="elsevierStyleSup">28&#8211;30</span></a> However discrepant results were found in other studies regarding the relationship between antioxidant <span class="elsevierStyleItalic">status</span> and pulmonary functions in COPD patients&#46;<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">14</span></a> Other studies failed to find a protective effect of antioxidants on the lung function&#46;<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">30</span></a> The molecular mechanisms relating to antioxidant defence in COPD is still lacking&#46;<a class="elsevierStyleCrossRefs" href="#bib0155"><span class="elsevierStyleSup">31&#44;32</span></a> In this study we found that COPD patients had a significant &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41; decrease in antioxidant <span class="elsevierStyleItalic">status</span> &#40;vitamin C and sulphydryl groups&#41; when compared with control group&#46; The significant decrease in vitamin C and sulphydryl groups associated to COPD patients agrees with the hypothesis that glutathione&#44; the most abundant cellular non-protein sulphydryls&#44; and the glutathione redox cycle may be regarded as an antioxidant mechanism involved in the protection against oxidative stress&#46;<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">33</span></a> We did not find a significant difference in the antioxidant <span class="elsevierStyleItalic">status</span> between non-smokers and smokers healthy subjects and between non-smokers and ex-smokers patients but we did observe a tendency toward a decrease in vitamin C associated to COPD patients with smoking habits&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">The difference of age between controls and COPD patients could be a limitation in our study&#46; In order to observe the effect of age&#44; we compared the results in healthy subjects younger than 50 years and those over 50 years&#46; We found no significant difference in plasma MDA levels and antioxidant <span class="elsevierStyleItalic">status</span> associated to age&#46; Our observation is supported by the studies of other authors who found no relationship between plasma antioxidant capacity and protein sulphydryls with age in healthy subjects&#46;<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">21&#44;28</span></a> Other studies reported no age dependent change in the plasma MDA levels in the general population&#46;<a class="elsevierStyleCrossRefs" href="#bib0170"><span class="elsevierStyleSup">34&#44;35</span></a> In addition&#44; other authors have found a marked reduction in MDA associated to chronic airway disorders&#44; particularly COPD&#46; It has been suggested that the increase in MDA levels related to the pathophysiology of COPD may be a promising marker of oxidative stress in chronic pulmonary diseases&#46;<a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">17</span></a></p><p id="par0080" class="elsevierStylePara elsevierViewall">In our study oxygen therapy was not analyzed&#46; However the effect of this therapy in oxidative stress in COPD patients is still controversial&#46; Our results show a significant difference in oxidative stress and antioxidant <span class="elsevierStyleItalic">status</span> between controls and COPD patients &#40;with or without oxygen therapy&#41;&#46; No significant difference was found to be associated with oxygen therapy&#46; Further studies are required to clarify the effects of oxygen therapy in COPD patients&#46;</p><p id="par0085" class="elsevierStylePara elsevierViewall">In conclusion&#44; this study showed that elevated levels of TBARS&#44; namely the end product of oxidant stress appears to be more pronounced in COPD patients&#46; Concomitantly&#44; a significant decrease in the protective antioxidant responses was found to be associated to COPD in both non-smokers and ex-smokers patients&#46; The imbalance between oxidants and antioxidants seems to be an important event associated to the development of COPD&#46; This could provide therapeutic implications in the future&#46;</p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Conflicts of interest</span><p id="par0090" class="elsevierStylePara elsevierViewall">The authors have no conflicts of interest to declare&#46;</p></span></span>"
    "textoCompletoSecciones" => array:1 [
      "secciones" => array:11 [
        0 => array:2 [
          "identificador" => "xres175788"
          "titulo" => "Abstract"
        ]
        1 => array:2 [
          "identificador" => "xpalclavsec164122"
          "titulo" => "Keywords"
        ]
        2 => array:2 [
          "identificador" => "xres175789"
          "titulo" => "Resumo"
        ]
        3 => array:2 [
          "identificador" => "xpalclavsec164123"
          "titulo" => "Palavras-chave"
        ]
        4 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Introduction"
        ]
        5 => array:3 [
          "identificador" => "sec0010"
          "titulo" => "Materials and methods"
          "secciones" => array:3 [
            0 => array:2 [
              "identificador" => "sec0015"
              "titulo" => "Study population"
            ]
            1 => array:3 [
              "identificador" => "sec0020"
              "titulo" => "Methods"
              "secciones" => array:3 [
                0 => array:2 [
                  "identificador" => "sec0025"
                  "titulo" => "Determination of plasmatic malonyldialdehyde"
                ]
                1 => array:2 [
                  "identificador" => "sec0030"
                  "titulo" => "Quantification of vitamin C"
                ]
                2 => array:2 [
                  "identificador" => "sec0035"
                  "titulo" => "Determination of non-protein sulphydryl groups"
                ]
              ]
            ]
            2 => array:2 [
              "identificador" => "sec0040"
              "titulo" => "Statistical analyses"
            ]
          ]
        ]
        6 => array:3 [
          "identificador" => "sec0045"
          "titulo" => "Results"
          "secciones" => array:1 [
            0 => array:2 [
              "identificador" => "sec0050"
              "titulo" => "Oxidative stress and antioxidant status"
            ]
          ]
        ]
        7 => array:2 [
          "identificador" => "sec0055"
          "titulo" => "Discussion and conclusions"
        ]
        8 => array:2 [
          "identificador" => "sec0060"
          "titulo" => "Conflicts of interest"
        ]
        9 => array:2 [
          "identificador" => "xack45053"
          "titulo" => "Acknowledgements"
        ]
        10 => array:1 [
          "titulo" => "References"
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2012-02-20"
    "fechaAceptado" => "2012-09-14"
    "PalabrasClave" => array:2 [
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec164122"
          "palabras" => array:4 [
            0 => "Chronic obstructive pulmonary disease"
            1 => "Oxidative stress"
            2 => "Lipid peroxidation"
            3 => "Antioxidants"
          ]
        ]
      ]
      "pt" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palavras-chave"
          "identificador" => "xpalclavsec164123"
          "palabras" => array:4 [
            0 => "Doen&#231;a pulmonar obstrutiva cr&#243;nica"
            1 => "<span class="elsevierStyleItalic">Stress</span> oxidativo"
            2 => "Peroxida&#231;&#227;o lip&#237;dica"
            3 => "Antioxidantes"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:2 [
      "en" => array:2 [
        "titulo" => "Abstract"
        "resumen" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Chronic obstructive pulmonary disease &#40;COPD&#41; is one of the most common chronic diseases and a major cause of morbidity and mortality&#46; An imbalance between oxidants and antioxidants &#40;oxidative stress&#41; has been proposed as a critical event in the pathogenesis of COPD&#46; The increased oxidative stress in patients with COPD is the result of exogenous oxidants namely pollutants and cigarette smoke as well as endogenous oxidant production during inflammation&#46; The aim of the present study was to clarify the hypothesis about the presence of an imbalance between oxidants and the antioxidant defences associated to COPD&#46; In this study&#44; we evaluated a biomarker of oxidative stress &#40;malondialdehyde&#44; a lipid peroxidation derived product&#41; and non-enzymatic antioxidants &#40;vitamin C and the sulphydryl groups&#41; in COPD patients and healthy controls&#46; The marker of oxidative stress was found to be significantly &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41; higher in COPD patients when compared with control group&#46; No age dependent changes in the plasma levels of lipid peroxidation products were found&#46; COPD patients had a significant &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41; decrease in antioxidant <span class="elsevierStyleItalic">status</span> as compared with control group&#46; Our results show that oxidative stress is an important pathophysiologic change in COPD&#46;</p>"
      ]
      "pt" => array:2 [
        "titulo" => "Resumo"
        "resumen" => "<p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">A doen&#231;a pulmonar obstrutiva cr&#243;nica &#40;DPOC&#41; &#233; uma das doen&#231;as cr&#243;nicas mais comuns e representa uma importante causa de morbilidade e mortalidade&#46; Um desequil&#237;brio entre oxidantes e antioxidantes &#40;<span class="elsevierStyleItalic">stress</span> oxidativo&#41; tem sido proposto como um acontecimento importante na patog&#233;nese da DPOC&#46; O aumento do <span class="elsevierStyleItalic">stress</span> oxidativo em doentes com DPOC &#233; o resultado da presen&#231;a de oxidantes ex&#243;genos&#44; nomeadamente&#44; poluentes e fumo do tabaco&#44; assim como oxidantes end&#243;genos produzidos durante a inflama&#231;&#227;o&#46; O objetivo do presente estudo consistiu em clarificar a hip&#243;tese sobre a exist&#234;ncia de um desequil&#237;brio entre oxidantes e as defesas antioxidantes associado &#224; DPOC&#46; Neste estudo&#44; avaliou-se um biomarcador do <span class="elsevierStyleItalic">stress</span> oxidativo &#40;malonildialde&#237;do&#44; um produto resultante da peroxida&#231;&#227;o lip&#237;dica&#41; e antioxidantes n&#227;o-enzim&#225;ticos &#40;vitamina C e grupos sulfidrilo&#41;&#44; em doentes com DPOC e em controlos saud&#225;veis&#46; Observou-se um aumento significativo &#40;p<span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#44;001&#41; do marcador do <span class="elsevierStyleItalic">stress</span> oxidativo nos doentes com DPOC comparativamente ao grupo controlo&#46; N&#227;o foram encontradas altera&#231;&#245;es dependentes da idade nos n&#237;veis dos produtos da peroxida&#231;&#227;o lip&#237;dica&#46; Os doentes DPOC apresentaram uma diminui&#231;&#227;o significativa &#40;p<span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#44;001&#41; do <span class="elsevierStyleItalic">status</span> antioxidante&#44; comparativamente ao grupo controlo&#46; Os nossos resultados evidenciam que o <span class="elsevierStyleItalic">stress</span> oxidativo representa uma importante altera&#231;&#227;o fisiopatol&#243;gica na DPOC&#46;</p>"
      ]
    ]
    "NotaPie" => array:2 [
      0 => array:2 [
        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara">Please cite this article as&#58; Crist&#243;v&#227;o C&#44; et al&#46; Avalia&#231;&#227;o do equil&#237;brio entre oxidantes e antioxidantes na patog&#233;nese da doen&#231;a pulmonar obstrutiva cr&#243;nica&#46; Rev Port Pneumol&#46; 2013&#46; <span class="elsevierStyleInterRef" href="doi:10.1016/j.rppneu.2012.09.002">http&#58;&#47;&#47;dx&#46;doi&#46;org&#47;10&#46;1016&#47;j&#46;rppneu&#46;2012&#46;09&#46;002</span>&#46;</p>"
      ]
      1 => array:2 [
        "etiqueta" => "&#9734;&#9734;"
        "nota" => "<p class="elsevierStyleNotepara">Department where the study was carried out&#58; This study was conducted in the laboratory of Genetics&#44; Faculdade de Medicina&#44; Universidade de Lisboa and in the laboratories of the Universidade Lus&#243;fona de Humanidades e Tecnologias de Lisboa&#46;</p>"
      ]
    ]
    "multimedia" => array:4 [
      0 => array:7 [
        "identificador" => "fig0005"
        "etiqueta" => "Figure 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
            "Alto" => 818
            "Ancho" => 1382
            "Tamanyo" => 60403
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Levels of malondialdehyde &#40;MDA&#41; in plasma from control subjects and COPD patients&#46; Values are expressed as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEM&#46; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001 significantly different between controls and whole COPD group&#46; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01 significantly different between controls and non-smoker patients&#46; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001 significantly different between controls and ex-smoker patients&#46; There was no significant differences between non-smokers and ex-smokers COPD patients&#46;</p>"
        ]
      ]
      1 => array:7 [
        "identificador" => "fig0010"
        "etiqueta" => "Figure 2"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr2.jpeg"
            "Alto" => 974
            "Ancho" => 2295
            "Tamanyo" => 110836
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Concentration of plasma vitamin C and sulphydryl groups in control subjects and COPD patients&#46; Values are expressed as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEM&#46; COPD patients had a significant &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;001&#41; decrease in antioxidant <span class="elsevierStyleItalic">status</span> &#40;vitamin C and <span class="elsevierStyleGlyphsbnd"></span>SH groups&#41; when compared with control group&#46;</p>"
        ]
      ]
      2 => array:7 [
        "identificador" => "tbl0005"
        "etiqueta" => "Table 1"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:1 [
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">Clinical and physiological parameters&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">Values&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Age&#44; years&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">71&#44;30<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>7&#44;68&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Male&#47;female&#44; <span class="elsevierStyleItalic">n</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">11&#47;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Body mass index&#44; kg&#47;m<span class="elsevierStyleSup">2</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">27&#44;918<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#44;724&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Smoking status&#58; ex-smokers&#47;non-smokers&#44; <span class="elsevierStyleItalic">n</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">15&#47;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Packs-years in ex-smokers&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">68&#44;8<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>10&#44;754&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">GOLD stage&#58; I&#47;II&#47;IV&#44; <span class="elsevierStyleItalic">n</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">2&#47;4&#47;14&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">FVC&#44; &#37; predicted&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">77&#44;100<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>5&#44;190&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">FEV1&#44; &#37; predicted&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">58&#44;130<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>5&#44;332&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">FEV1&#47;FVC&#44; &#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">59&#44;453<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>3&#44;805&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">paO2&#44; mmHg&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">66&#44;700<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2&#44;71&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab268168.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Clinical and physiological characteristics of COPD group&#46;</p>"
        ]
      ]
      3 => array:7 [
        "identificador" => "tbl0010"
        "etiqueta" => "Table 2"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">All data are expressed as mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>SEM&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">Controls &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>50&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">Whole COPD group &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>20&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">COPD non-smokers &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>5&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" style="border-bottom: 2px solid black">COPD ex-smokers &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>15&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">MDA &#40;nmol&#47;mL&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">3&#46;830<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;310&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">8&#46;549<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#46;228&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">7&#46;268<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2&#46;309&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">8&#46;976<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>1&#46;472&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">VitaminC &#40;&#956;g&#47;mL&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">6&#46;230<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;220&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">1&#46;866<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;353&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">2&#46;119<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;770&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">1&#46;782<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;408&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Sulphydryl groups &#40;&#956;mol&#47;mL&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;275<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;011&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;135<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;022&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;130<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;021&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">0&#46;137<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>0&#46;029&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab268167.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Plasma MDA levels and antioxidant <span class="elsevierStyleItalic">status</span> of the study groups&#46;</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0005"
          "bibliografiaReferencia" => array:35 [
            0 => array:3 [
              "identificador" => "bib0005"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Oxidative stress in chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:3 [
                          "colaboracion" => "Oxidative Stress Study Group"
                          "etal" => false
                          "autores" => array:3 [
                            0 => "J&#46;E&#46; Repine"
                            1 => "A&#46; Bast"
                            2 => "I&#46; Lankhorst"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/ajrccm.156.2.9611013"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "1997"
                        "volumen" => "156"
                        "paginaInicial" => "341"
                        "paginaFinal" => "357"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9279209"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0010"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The pathobiology of chronic bronchitis and emphysema"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "R&#46;M&#46; Tuder"
                            1 => "N&#46;F&#46; Voelkel"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "LibroEditado" => array:4 [
                        "titulo" => "Chronic obstructive lung diseases"
                        "paginaInicial" => "90"
                        "paginaFinal" => "113"
                        "serieFecha" => "2002"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0015"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Epidemiology"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "J&#46; Vestbo"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "LibroEditado" => array:4 [
                        "titulo" => "Chronic obstructive lung diseases"
                        "paginaInicial" => "41"
                        "paginaFinal" => "55"
                        "serieFecha" => "2002"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib0020"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Oxidant&#47;antioxidant imbalance in smokers and chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "I&#46; Rahman"
                            1 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Thorax"
                        "fecha" => "1996"
                        "volumen" => "51"
                        "paginaInicial" => "348"
                        "paginaFinal" => "350"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8733482"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib0025"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Oxidants and antioxidants as therapeutic targets in chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "W&#46; MacNee"
                            1 => "I&#46; Rahman"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/ajrccm.160.supplement_1.15"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "1999"
                        "volumen" => "160"
                        "paginaInicial" => "S58"
                        "paginaFinal" => "S65"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10556172"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib0030"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Oxidants&#47;antioxidants and COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Chest"
                        "fecha" => "2000"
                        "volumen" => "117"
                        "paginaInicial" => "303S"
                        "paginaFinal" => "317S"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10843965"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib0035"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Oxidant&#8211;antioxidant balance in patients with COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "I&#46; Hanta"
                            1 => "A&#46; Kocabas"
                            2 => "N&#46; Canacankatan"
                            3 => "S&#46; Kuleci"
                            4 => "G&#46; Seydaoglu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1007/s00408-005-2561-4"
                      "Revista" => array:6 [
                        "tituloSerie" => "Lung"
                        "fecha" => "2006"
                        "volumen" => "184"
                        "paginaInicial" => "51"
                        "paginaFinal" => "55"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16622773"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib0040"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Impact of oxidative stress on lung diseases"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "H&#46;S&#46; Park"
                            1 => "S&#46;R&#46; Kim"
                            2 => "Y&#46;C&#46; Lee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/j.1440-1843.2008.01447.x"
                      "Revista" => array:6 [
                        "tituloSerie" => "Respirology"
                        "fecha" => "2009"
                        "volumen" => "14"
                        "paginaInicial" => "27"
                        "paginaFinal" => "38"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19144046"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib0045"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "ROS in the local and systemic pathogenesis of COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "R&#46;C&#46;J&#46; Langen"
                            1 => "S&#46;H&#46; Korn"
                            2 => "E&#46;F&#46;M&#46; Wouters"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Free Radic Biol Med"
                        "fecha" => "2003"
                        "volumen" => "35"
                        "paginaInicial" => "226"
                        "paginaFinal" => "235"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12885585"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib0050"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pathogenesis of chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1513/pats.200504-045SR"
                      "Revista" => array:6 [
                        "tituloSerie" => "Proc Am Thorac Soc"
                        "fecha" => "2005"
                        "volumen" => "2"
                        "paginaInicial" => "258"
                        "paginaFinal" => "266"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16267346"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib0055"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Systemic manifestations in chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "V&#46;Z&#46; Dourado"
                            1 => "S&#46;E&#46; Tanni"
                            2 => "S&#46;A&#46; Vale"
                            3 => "M&#46;M&#46; Faganello"
                            4 => "F&#46;F&#46; Sanchez"
                            5 => "I&#46; Godoy"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Bras Pneumol"
                        "fecha" => "2006"
                        "volumen" => "32"
                        "paginaInicial" => "161"
                        "paginaFinal" => "171"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17273586"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            11 => array:3 [
              "identificador" => "bib0060"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pathogenic triad in COPD&#58; oxidative stress&#44; protease&#8211;antiprotease imbalance&#44; and inflammation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "B&#46;M&#46; Fischer"
                            1 => "E&#46; Pavlisko"
                            2 => "J&#46;A&#46; Voynow"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.2147/COPD.S10770"
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Chron Obstruct Pulmon Dis"
                        "fecha" => "2011"
                        "volumen" => "6"
                        "paginaInicial" => "413"
                        "paginaFinal" => "421"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/21857781"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib0065"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pulmonary and systemic oxidant&#47;antioxidant imbalance in chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1513/pats.200411-056SF"
                      "Revista" => array:6 [
                        "tituloSerie" => "Proc Am Thorac Soc"
                        "fecha" => "2005"
                        "volumen" => "2"
                        "paginaInicial" => "50"
                        "paginaFinal" => "60"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16113469"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib0070"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The association between oxidative stress and obstructive lung impairment in patients with COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "Z&#46; Kluchov&#225;"
                            1 => "D&#46; Petr&#225;sova"
                            2 => "P&#46; Joppa"
                            3 => "Z&#46; Dorkov&#225;"
                            4 => "R&#46; Tk&#225;cov&#225;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Phys Res"
                        "fecha" => "2007"
                        "volumen" => "56"
                        "paginaInicial" => "51"
                        "paginaFinal" => "56"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib0075"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Pathogenesis of COPD&#46; Part II&#46; Oxidative&#8211;antioxidative imbalance"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "J&#46;C&#46;W&#46; Mak"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2008"
                        "volumen" => "12"
                        "paginaInicial" => "368"
                        "paginaFinal" => "374"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18371260"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            15 => array:3 [
              "identificador" => "bib0080"
              "etiqueta" => "16"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lipid peroxidation and antioxidants as biomarkers of tissue damage"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "J&#46;M&#46;C&#46; Gutteridge"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Clin Chem"
                        "fecha" => "1995"
                        "volumen" => "41"
                        "paginaInicial" => "1819"
                        "paginaFinal" => "1828"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7497639"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            16 => array:3 [
              "identificador" => "bib0085"
              "etiqueta" => "17"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Malondialdehyde in exhaled breath condensate as a marker of oxidative stress in different pulmonary diseases"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "M&#46;L&#46; Bartoli"
                            1 => "F&#46; Novelli"
                            2 => "F&#46; Costa"
                            3 => "L&#46; Malagrin&#242;"
                            4 => "L&#46; Melosini"
                            5 => "E&#46; Bacci"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:4 [
                        "tituloSerie" => "Mediators Inflamm"
                        "fecha" => "2011"
                        "paginaInicial" => "1"
                        "paginaFinal" => "6"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            17 => array:3 [
              "identificador" => "bib0090"
              "etiqueta" => "18"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "DNA damage and oxygen species"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "J&#46; Rueff"
                            1 => "A&#46; Laires"
                            2 => "A&#46; Br&#225;s"
                            3 => "H&#46; Borba"
                            4 => "T&#46; Chaveca"
                            5 => "J&#46; Gaspar"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Libro" => array:4 [
                        "titulo" => "DNA repair mechanisms and their biological implications in mammalian cells"
                        "fecha" => "1989"
                        "editorial" => "Plenum Press"
                        "editorialLocalizacion" => "New York"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            18 => array:3 [
              "identificador" => "bib0095"
              "etiqueta" => "19"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Systemic effects of smoking"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "D&#46;G&#46; Yanbaeva"
                            1 => "M&#46;A&#46; Dentener"
                            2 => "E&#46;C&#46; Creutzberg"
                            3 => "G&#46; Wesselin"
                            4 => "E&#46;F&#46;M&#46; Wouters"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1378/chest.06-2179"
                      "Revista" => array:6 [
                        "tituloSerie" => "Chest"
                        "fecha" => "2007"
                        "volumen" => "131"
                        "paginaInicial" => "1557"
                        "paginaFinal" => "1566"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17494805"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            19 => array:3 [
              "identificador" => "bib0100"
              "etiqueta" => "20"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Blood reflects tissue oxidative stress depending on biomarker and tissue studied"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "A&#46;S&#46; Veskoukis"
                            1 => "M&#46;G&#46; Nikolaidis"
                            2 => "A&#46; Kyparos"
                            3 => "D&#46; Kouretas"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.freeradbiomed.2009.07.014"
                      "Revista" => array:6 [
                        "tituloSerie" => "Free Radic Biol Med"
                        "fecha" => "2009"
                        "volumen" => "47"
                        "paginaInicial" => "1371"
                        "paginaFinal" => "1374"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19616614"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            20 => array:3 [
              "identificador" => "bib0105"
              "etiqueta" => "21"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Is there any relationship between plasma antioxidant capacity and lung function in smokers and in patients with chronic obstructive pulmonary disease&#63;"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "I&#46; Rahman"
                            1 => "E&#46; Swarska"
                            2 => "M&#46; Henry"
                            3 => "J&#46; Stolk"
                            4 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Thorax"
                        "fecha" => "2000"
                        "volumen" => "55"
                        "paginaInicial" => "189"
                        "paginaFinal" => "193"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10679536"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            21 => array:3 [
              "identificador" => "bib0110"
              "etiqueta" => "22"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "H&#46; Ohkawa"
                            1 => "N&#46; Ohishi"
                            2 => "K&#46; Yagi"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Anal Biochem"
                        "fecha" => "1979"
                        "volumen" => "95"
                        "paginaInicial" => "351"
                        "paginaFinal" => "358"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/36810"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            22 => array:3 [
              "identificador" => "bib0115"
              "etiqueta" => "23"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Selected methods for determination of ascorbic acid in animal cells&#44; tissues and fluids"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "S&#46;T&#46; Omaye"
                            1 => "J&#46;D&#46; Turnbull"
                            2 => "H&#46;E&#46; Sauberlich"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "LibroEditado" => array:4 [
                        "titulo" => "Methods enzymol&#44; vitamins and coenzymes"
                        "paginaInicial" => "3"
                        "paginaFinal" => "11"
                        "serieFecha" => "1979"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            23 => array:3 [
              "identificador" => "bib0120"
              "etiqueta" => "24"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Techniques in free radical research"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "C&#46; Rice-Evans"
                            1 => "A&#46; Diplock"
                            2 => "C&#46; Symons"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "LibroEditado" => array:4 [
                        "titulo" => "Laboratory techniques in biochemistry and molecular biology"
                        "paginaInicial" => "227"
                        "paginaFinal" => "229"
                        "serieFecha" => "1991"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            24 => array:3 [
              "identificador" => "bib0125"
              "etiqueta" => "25"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "4-Hydroxy-2-nonenal&#44; a specific lipid peroxidation product&#44; is elevated in lungs of patients with chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "I&#46; Rahman"
                            1 => "A&#46;A&#46;M&#46; van Schadewijk"
                            2 => "A&#46;J&#46;L&#46; Crowther"
                            3 => "P&#46;S&#46; Hiemstra"
                            4 => "J&#46; Stolk"
                            5 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/rccm.2110101"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "2002"
                        "volumen" => "166"
                        "paginaInicial" => "490"
                        "paginaFinal" => "495"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12186826"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            25 => array:3 [
              "identificador" => "bib0130"
              "etiqueta" => "26"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Increased content of thiobarbituric acid-reactive substances and hydrogen peroxide in the expired breath condensate of patients with stable chronic obstructive pulmonary disease&#58; no significant effect of cigarette smoking"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "D&#46; Nowak"
                            1 => "M&#46; Kasielski"
                            2 => "A&#46; Antczak"
                            3 => "T&#46; Pietras"
                            4 => "P&#46; Bialasiewicz"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1053/rmed.1999.0574"
                      "Revista" => array:6 [
                        "tituloSerie" => "Respir Med"
                        "fecha" => "1999"
                        "volumen" => "93"
                        "paginaInicial" => "389"
                        "paginaFinal" => "396"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10464820"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            26 => array:3 [
              "identificador" => "bib0135"
              "etiqueta" => "27"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Cigarette smoke oxidation of human plasma constituents"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "C&#46;E&#46; Cross"
                            1 => "C&#46;A&#46; O&#8217;Neill"
                            2 => "A&#46;Z&#46; Reznick"
                            3 => "M&#46;L&#46; Hu"
                            4 => "L&#46; Marcocci"
                            5 => "L&#46; Packer"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann N Y Acad Sci"
                        "fecha" => "1993"
                        "volumen" => "686"
                        "paginaInicial" => "72"
                        "paginaFinal" => "90"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8512263"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            27 => array:3 [
              "identificador" => "bib0140"
              "etiqueta" => "28"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Systemic oxidative stress in asthma&#44; COPD&#44; and smokers"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "I&#46; Rahman"
                            1 => "D&#46; Morrison"
                            2 => "K&#46; Donaldson"
                            3 => "W&#46; MacNee"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Am J Respir Crit Med"
                        "fecha" => "1996"
                        "volumen" => "154"
                        "paginaInicial" => "1055"
                        "paginaFinal" => "1060"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            28 => array:3 [
              "identificador" => "bib0145"
              "etiqueta" => "29"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The effects of antioxidants on exercise-induced lipid peroxidation in patients with COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "A&#46; Agacdiken"
                            1 => "I&#46; Basyigit"
                            2 => "M&#46; &#214;zden"
                            3 => "F&#46; Yildiz"
                            4 => "D&#46; Ural"
                            5 => "H&#46; Maral"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/j.1440-1843.2003.00526.x"
                      "Revista" => array:6 [
                        "tituloSerie" => "Respirology"
                        "fecha" => "2004"
                        "volumen" => "9"
                        "paginaInicial" => "38"
                        "paginaFinal" => "42"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/14982600"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            29 => array:3 [
              "identificador" => "bib0150"
              "etiqueta" => "30"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Increased oxidative stress and altered levels of antioxidants in chronic obstructive pulmonary disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "A&#46; Nadeem"
                            1 => "H&#46;G&#46; Raj"
                            2 => "S&#46;K&#46; Chhabra"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1007/s10753-006-8965-3"
                      "Revista" => array:6 [
                        "tituloSerie" => "Inflammation"
                        "fecha" => "2005"
                        "volumen" => "29"
                        "paginaInicial" => "23"
                        "paginaFinal" => "32"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16502343"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            30 => array:3 [
              "identificador" => "bib0155"
              "etiqueta" => "31"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The pharmokinetic limitations of antioxidant treatment for COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "R&#46; Foronjy"
                            1 => "A&#46; Wallace"
                            2 => "J&#46; d&#8217;Armiento"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.pupt.2007.10.004"
                      "Revista" => array:6 [
                        "tituloSerie" => "Pulm Pharmacol Ther"
                        "fecha" => "2008"
                        "volumen" => "21"
                        "paginaInicial" => "370"
                        "paginaFinal" => "379"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18054260"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            31 => array:3 [
              "identificador" => "bib0160"
              "etiqueta" => "32"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Antioxidant therapeutic advances in COPD"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "I&#46; Rahman"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1177/1753465808098224"
                      "Revista" => array:6 [
                        "tituloSerie" => "Ther Adv Respir Dis"
                        "fecha" => "2008"
                        "volumen" => "2"
                        "paginaInicial" => "351"
                        "paginaFinal" => "374"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19124382"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            32 => array:3 [
              "identificador" => "bib0165"
              "etiqueta" => "33"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Aging adversely affects the cigarette smoke-induced glutathione adaptive response in the lung"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "N&#46;S&#46; Gould"
                            1 => "E&#46; Min"
                            2 => "S&#46; Gauthier"
                            3 => "H&#46;W&#46; Chu"
                            4 => "R&#46; Martin"
                            5 => "B&#46;J&#46; Day"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1164/rccm.201003-0442OC"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Respir Crit Care Med"
                        "fecha" => "2010"
                        "volumen" => "182"
                        "paginaInicial" => "1114"
                        "paginaFinal" => "1122"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20622027"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            33 => array:3 [
              "identificador" => "bib0170"
              "etiqueta" => "34"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Age&#44; sex&#44; cigarette smoking and indices of free radical activity in healthy humans"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "A&#46;B&#46; Bridges"
                            1 => "N&#46;A&#46; Scott"
                            2 => "G&#46;J&#46; Parry"
                            3 => "J&#46;J&#46; Belch"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Eur J Med"
                        "fecha" => "1993"
                        "volumen" => "2"
                        "paginaInicial" => "205"
                        "paginaFinal" => "208"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8261071"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            34 => array:3 [
              "identificador" => "bib0175"
              "etiqueta" => "35"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Factors associated with oxidative stress in human populations"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "G&#46; Block"
                            1 => "M&#46; Dietrich"
                            2 => "E&#46;P&#46; Norkus"
                            3 => "J&#46;D&#46; Morrow"
                            4 => "M&#46; Hudes"
                            5 => "B&#46; Caan"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Epidemiol"
                        "fecha" => "2002"
                        "volumen" => "156"
                        "paginaInicial" => "274"
                        "paginaFinal" => "285"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12142263"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
    "agradecimientos" => array:1 [
      0 => array:3 [
        "identificador" => "xack45053"
        "titulo" => "Acknowledgements"
        "texto" => "<p id="par0095" class="elsevierStylePara elsevierViewall">We thank Professor J&#46; Mexia who greatly contributed to the Statistical Analyses of this work&#46; We extended our appreciation to patients and the healthy volunteers who generously collaborated in this study&#46;</p>"
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/21735115/0000001900000002/v1_201305151603/S2173511513000171/v1_201305151603/en/main.assets"
  "Apartado" => array:4 [
    "identificador" => "9710"
    "tipo" => "SECCION"
    "en" => array:2 [
      "titulo" => "Original articles"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "en"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/21735115/0000001900000002/v1_201305151603/S2173511513000171/v1_201305151603/en/main.pdf?idApp=UINPBA00004E&text.app=https://journalpulmonology.org/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511513000171?idApp=UINPBA00004E"
]
Article information
ISSN: 21735115
Original language: English
The statistics are updated each day
Year/Month Html Pdf Total
2024 November 6 3 9
2024 October 29 30 59
2024 September 32 24 56
2024 August 52 34 86
2024 July 32 26 58
2024 June 29 26 55
2024 May 35 35 70
2024 April 20 27 47
2024 March 36 21 57
2024 February 23 27 50
2024 January 20 26 46
2023 December 23 25 48
2023 November 22 32 54
2023 October 18 29 47
2023 September 19 33 52
2023 August 20 30 50
2023 July 23 22 45
2023 June 25 10 35
2023 May 25 24 49
2023 April 16 9 25
Show all

Follow this link to access the full text of the article

Pulmonology

Are you a health professional able to prescribe or dispense drugs?