Genotypic analysis of Mycobacterium tuberculosis isolates from a Lisbon hospital in Portugal
Análise genotípica de isolados de Mycobacterium tuberculosis de um hospital em Lisboa, Portugal
João Perdigão1, Catarina Milho2, Lurdes Carrilho3, Laura Brum4, Isabel Portugal5,
Corresponding author
isabel.portugal@ff.ul.pt
Correspondence to/Correspondência: Centro de Patogénese Molecular, URIA, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
Correspondence to/Correspondência: Centro de Patogénese Molecular, URIA, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal.
1 Licenciado em biologia microbiana e genética, mestre em biologia molecular e genética, Aluno de doutoramento em microbiologia/BsC, Microbiology and Genetics. MsC Molecular Biology and Genetics. PhD Microbiology (partway)
2 Licenciada em microbiologia/BsC, Microbiology Molecular Centro de Patogénese Molecular, URIA, Faculdade de Farmácia da Universidade de Lisboa, Portugal/Molecular Pathogenesis Centre, URIA, School of Pharmacy, Universidade de Lisboa, Portugal
3 Secção de Micobactérias do Serviço de Patologia Clínica do Hospital de Pulido Valente, Lisboa, Portugal/Mycobacteria Section, Clinical Pathology Unit, Hospital de Pulido Valente, Lisboa, Portugal
4 Professora auxiliar convidada da Faculdade de Ciências Médicas da Universidade Nova de Lisboa. Assessora de Bacteriologia do Instituto Nacional de Saúde Dr. Ricardo Jorge. Departamento de Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal/Assistant Guest Professor, School of Medical Sciences, Universidade Nova de Lisboa. Bacteriology assistant, Instituto Nacional de Saúde Dr. Ricardo Jorge. Department of Infectious Diseases, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal
5 Professora auxiliar da Faculdade de Farmácia da Universidade de Lisboa. Investigadora visitante do Instituto Nacional de Saúde Dr. Ricardo Jorge, Centro de Patogénese Molecular, Faculdade de Farmácia da Universidade de Lisboa, Portugal. Departamento de Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal/Guest Professor, School of Pharmacy, Universidade de Lisboa. Visiting Researcher, Instituto Nacional de Saúde Dr. Ricardo Jorge, Molecular Pathogenesis Centre, School of Pharmacy, Universidade de Lisboa, Portugal. Department of Infectious Diseases, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal
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Assessora de Bacteriologia do Instituto Nacional de Saúde Dr. Ricardo Jorge. Departamento de Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal/Assistant Guest Professor, School of Medical Sciences, Universidade Nova de Lisboa. Bacteriology assistant, Instituto Nacional de Saúde Dr. Ricardo Jorge. Department of Infectious Diseases, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal" "etiqueta" => "<span class="elsevierStyleSup">4</span>" "identificador" => "aff0020" ] 4 => array:3 [ "entidad" => "Professora auxiliar da Faculdade de Farmácia da Universidade de Lisboa. Investigadora visitante do Instituto Nacional de Saúde Dr. Ricardo Jorge, Centro de Patogénese Molecular, Faculdade de Farmácia da Universidade de Lisboa, Portugal. Departamento de Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal/Guest Professor, School of Pharmacy, Universidade de Lisboa. Visiting Researcher, Instituto Nacional de Saúde Dr. Ricardo Jorge, Molecular Pathogenesis Centre, School of Pharmacy, Universidade de Lisboa, Portugal. Department of Infectious Diseases, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal" "etiqueta" => "<span class="elsevierStyleSup">5</span>" "identificador" => "aff0025" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Correspondence to/Correspondência: Centro de Patogénese Molecular, URIA, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal." ] ] ] ] "titulosAlternativos" => array:1 [ "pt" => array:1 [ "titulo" => "Análise genotípica de isolados de <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> de um hospital em Lisboa, Portugal" ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2009-03-30" "fechaAceptado" => "2009-04-17" "PalabrasClave" => array:2 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Key-words" "identificador" => "xpalclavsec160298" "palabras" => array:4 [ 0 => "Lisboa family" 1 => "tuberculosis" 2 => "multidrug resistance" 3 => "MIRU" ] ] ] "pt" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palavras-chave" "identificador" => "xpalclavsec160299" "palabras" => array:4 [ 0 => "Família Lisboa" 1 => "tuberculose" 2 => "multirresistência" 3 => "MIRU" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "en" => array:2 [ "titulo" => "Abstract" "resumen" => "<p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Portugal has one of the highest tuberculosis notification rates of the European Union with Lisbon Health Region having an incidence rate well above the national average. The present study analyses the transmission, drug susceptibility and characteristics of a study population from a Central Lisbon's Hospital. One hundred and thirty-two <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> clinical isolates were previously tested for drug susceptibility to first-line drugs. The multidrug (MDR) resistance rate was found to be 3.0%, while 13.6% of the isolates were resistant to one or more first-line drugs. HIV serology was available for 98 patients, 26 (26.5%) were positive. Genotyping was performed by MIRU-VNTR and 53 (40,2%) out of the 132 isolates were found to be distributed through 17 MIRU-VNTR clusters of two or more isolates. Lisboa strains accounted for 25.8% of all strains. We conclude that transmission of resistant and susceptible <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> strains is occurring, with special concern for Lisboa strains.</p>" ] "pt" => array:2 [ "titulo" => "Resumo" "resumen" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Portugal apresenta uma das taxas de incidência de tuberculose mais altas da União Europeia, tendo a Região de Saúde de Lisboa uma taxa de incidência muito acima da média nacional. O presente estudo analisa a transmissão, susceptibilidade aos antibacilares e características de uma população de estudo de um hospital central de Lisboa. Cento e trinta e dois isolados clínicos de <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> foram previamente testados quanto à susceptibilidade aos antibacilares de primeira linha. A taxa de multirresistência encontrada foi de 3,0%, enquanto 13,6% dos isolados eram resistentes a um ou mais antibacilares de primeira linha. A serologia para o VIH estava disponível para 98 doentes, 26 (26,5%) eram positivos. Os isolados foram genotipados por MIRU-VNTR e 53 (40,2%) dos 132 isolados encontravamse distribuídos por 17 <span class="elsevierStyleItalic">clusters</span> MIRU-VNTR diferentes de dois ou mais isolados. De todos os isolados analisados, 25,8% pertenciam à família Lisboa. Concluímos que a transmissão de estirpes de <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>, resistentes e susceptíveis, está a ocorrer, com especial preocupação para as estirpes Lisboa.</p>" ] ] "bibliografia" => array:2 [ "titulo" => "Bibliography" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:27 [ 0 => array:3 [ "identificador" => "bib1" "etiqueta" => "1." "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Surveillance of tuberculosis cases in Europe. Report on tuberculosis cases notified in 2006. 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