Genotypic analysis of Mycobacterium tuberculosis isolates from a Lisbon hospital in Portugal

Perdigão, João; Milho, Catarina; Carrilho, Lurdes; Brum, Laura; Portugal, Isabel

doi:10.1016/S2173-5115(09)70153-8

Pulmonology

ISSN: 2531-0437

Pulmonology (previously Revista Portuguesa de Pneumologia) is the official journal of the Portuguese Society of Pulmonology (Sociedade Portuguesa de Pneumologia/SPP). The journal publishes 6 issues per year, mainly about respiratory system diseases in adults and clinical research. This work can range from peer-reviewed original articles to review articles, editorials, and opinion articles. The journal is printed in English, and is freely available in its web page as well as in Medline and other databases.

Special Issue 2023. Volume 29, Issue S4.

Indexed in:

Science Citation Index Expanded, Journal of Citation Reports; Index Medicus/MEDLINE; Scopus; EMBASE/Excerpta Medica

4 Professora auxiliar convidada da Faculdade de Ciências Médicas da Universidade Nova de Lisboa. Assessora de Bacteriologia do Instituto Nacional de Saúde Dr. Ricardo Jorge. Departamento de Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal/Assistant Guest Professor, School of Medical Sciences, Universidade Nova de Lisboa. Bacteriology assistant, Instituto Nacional de Saúde Dr. Ricardo Jorge. Department of Infectious Diseases, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal

5 Professora auxiliar da Faculdade de Farmácia da Universidade de Lisboa. Investigadora visitante do Instituto Nacional de Saúde Dr. Ricardo Jorge, Centro de Patogénese Molecular, Faculdade de Farmácia da Universidade de Lisboa, Portugal. Departamento de Doenças Infecciosas, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal/Guest Professor, School of Pharmacy, Universidade de Lisboa. Visiting Researcher, Instituto Nacional de Saúde Dr. Ricardo Jorge, Molecular Pathogenesis Centre, School of Pharmacy, Universidade de Lisboa, Portugal. Department of Infectious Diseases, Instituto Nacional de Saúde Dr. Ricardo Jorge, Lisboa, Portugal

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Abstract

Portugal has one of the highest tuberculosis notification rates of the European Union with Lisbon Health Region having an incidence rate well above the national average. The present study analyses the transmission, drug susceptibility and characteristics of a study population from a Central Lisbon's Hospital. One hundred and thirty-two Mycobacterium tuberculosis clinical isolates were previously tested for drug susceptibility to first-line drugs. The multidrug (MDR) resistance rate was found to be 3.0%, while 13.6% of the isolates were resistant to one or more first-line drugs. HIV serology was available for 98 patients, 26 (26.5%) were positive. Genotyping was performed by MIRU-VNTR and 53 (40,2%) out of the 132 isolates were found to be distributed through 17 MIRU-VNTR clusters of two or more isolates. Lisboa strains accounted for 25.8% of all strains. We conclude that transmission of resistant and susceptible Mycobacterium tuberculosis strains is occurring, with special concern for Lisboa strains.

Key-words:

Lisboa family

tuberculosis

multidrug resistance

MIRU

Resumo

Portugal apresenta uma das taxas de incidência de tuberculose mais altas da União Europeia, tendo a Região de Saúde de Lisboa uma taxa de incidência muito acima da média nacional. O presente estudo analisa a transmissão, susceptibilidade aos antibacilares e características de uma população de estudo de um hospital central de Lisboa. Cento e trinta e dois isolados clínicos de Mycobacterium tuberculosis foram previamente testados quanto à susceptibilidade aos antibacilares de primeira linha. A taxa de multirresistência encontrada foi de 3,0%, enquanto 13,6% dos isolados eram resistentes a um ou mais antibacilares de primeira linha. A serologia para o VIH estava disponível para 98 doentes, 26 (26,5%) eram positivos. Os isolados foram genotipados por MIRU-VNTR e 53 (40,2%) dos 132 isolados encontravamse distribuídos por 17 clusters MIRU-VNTR diferentes de dois ou mais isolados. De todos os isolados analisados, 25,8% pertenciam à família Lisboa. Concluímos que a transmissão de estirpes de Mycobacterium tuberculosis, resistentes e susceptíveis, está a ocorrer, com especial preocupação para as estirpes Lisboa.

Palavras-chave:

Família Lisboa

tuberculose

multirresistência

MIRU

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