array:24 [
  "pii" => "S2173511515000391"
  "issn" => "21735115"
  "doi" => "10.1016/j.rppnen.2014.09.010"
  "estado" => "S300"
  "fechaPublicacion" => "2015-07-01"
  "aid" => "1040"
  "copyright" => "Sociedade Portuguesa de Pneumologia"
  "copyrightAnyo" => "2014"
  "documento" => "article"
  "crossmark" => 1
  "licencia" => "http://www.elsevier.com/open-access/userlicense/1.0/"
  "subdocumento" => "fla"
  "cita" => "Rev Port Pneumol. 2015;21:185-91"
  "abierto" => array:3 [
    "ES" => true
    "ES2" => true
    "LATM" => true
  ]
  "gratuito" => true
  "lecturas" => array:2 [
    "total" => 1528
    "formatos" => array:3 [
      "EPUB" => 209
      "HTML" => 712
      "PDF" => 607
    ]
  ]
  "itemSiguiente" => array:19 [
    "pii" => "S2173511515000172"
    "issn" => "21735115"
    "doi" => "10.1016/j.rppnen.2014.12.003"
    "estado" => "S300"
    "fechaPublicacion" => "2015-07-01"
    "aid" => "1021"
    "copyright" => "Sociedade Portuguesa de Pneumologia"
    "documento" => "article"
    "crossmark" => 1
    "licencia" => "http://www.elsevier.com/open-access/userlicense/1.0/"
    "subdocumento" => "fla"
    "cita" => "Rev Port Pneumol. 2015;21:192-7"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 1503
      "formatos" => array:3 [
        "EPUB" => 207
        "HTML" => 645
        "PDF" => 651
      ]
    ]
    "en" => array:11 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>"
      "titulo" => "Respiratory parameters in elite athletes &#8211; does sport have an influence&#63;"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => "en"
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "192"
          "paginaFinal" => "197"
        ]
      ]
      "contieneResumen" => array:1 [
        "en" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "S&#46; Mazic, B&#46; Lazovic, M&#46; Djelic, J&#46; Suzic-Lazic, S&#46; Djordjevic-Saranovic, T&#46; Durmic, I&#46; Soldatovic, D&#46; Zikic, Z&#46; Gluvic, V&#46; Zugic"
          "autores" => array:10 [
            0 => array:2 [
              "nombre" => "S&#46;"
              "apellidos" => "Mazic"
            ]
            1 => array:2 [
              "nombre" => "B&#46;"
              "apellidos" => "Lazovic"
            ]
            2 => array:2 [
              "nombre" => "M&#46;"
              "apellidos" => "Djelic"
            ]
            3 => array:2 [
              "nombre" => "J&#46;"
              "apellidos" => "Suzic-Lazic"
            ]
            4 => array:2 [
              "nombre" => "S&#46;"
              "apellidos" => "Djordjevic-Saranovic"
            ]
            5 => array:2 [
              "nombre" => "T&#46;"
              "apellidos" => "Durmic"
            ]
            6 => array:2 [
              "nombre" => "I&#46;"
              "apellidos" => "Soldatovic"
            ]
            7 => array:2 [
              "nombre" => "D&#46;"
              "apellidos" => "Zikic"
            ]
            8 => array:2 [
              "nombre" => "Z&#46;"
              "apellidos" => "Gluvic"
            ]
            9 => array:2 [
              "nombre" => "V&#46;"
              "apellidos" => "Zugic"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511515000172?idApp=UINPBA00004E"
    "url" => "/21735115/0000002100000004/v1_201507020213/S2173511515000172/v1_201507020213/en/main.assets"
  ]
  "itemAnterior" => array:19 [
    "pii" => "S2173511514001237"
    "issn" => "21735115"
    "doi" => "10.1016/j.rppnen.2014.09.003"
    "estado" => "S300"
    "fechaPublicacion" => "2015-07-01"
    "aid" => "1003"
    "copyright" => "Sociedade Portuguesa de Pneumologia"
    "documento" => "article"
    "crossmark" => 1
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "fla"
    "cita" => "Rev Port Pneumol. 2015;21:178-84"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 1317
      "formatos" => array:3 [
        "EPUB" => 218
        "HTML" => 597
        "PDF" => 502
      ]
    ]
    "en" => array:11 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>"
      "titulo" => "Children&#39;s exposure to second hand smoke at home&#58; A cross-sectional study in Portugal"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => "en"
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "178"
          "paginaFinal" => "184"
        ]
      ]
      "contieneResumen" => array:1 [
        "en" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Paulo D&#46; Vit&#243;ria, Jos&#233; Cunha Machado, Ana Carolina Ara&#250;jo, Sofia B&#46; Ravara, Catarina Samorinha, Henedina Antunes, Manuel Rosas, Elisardo Beco&#241;a, Jos&#233; Precioso"
          "autores" => array:9 [
            0 => array:2 [
              "nombre" => "Paulo D&#46;"
              "apellidos" => "Vit&#243;ria"
            ]
            1 => array:2 [
              "nombre" => "Jos&#233; Cunha"
              "apellidos" => "Machado"
            ]
            2 => array:2 [
              "nombre" => "Ana Carolina"
              "apellidos" => "Ara&#250;jo"
            ]
            3 => array:2 [
              "nombre" => "Sofia B&#46;"
              "apellidos" => "Ravara"
            ]
            4 => array:2 [
              "nombre" => "Catarina"
              "apellidos" => "Samorinha"
            ]
            5 => array:2 [
              "nombre" => "Henedina"
              "apellidos" => "Antunes"
            ]
            6 => array:2 [
              "nombre" => "Manuel"
              "apellidos" => "Rosas"
            ]
            7 => array:2 [
              "nombre" => "Elisardo"
              "apellidos" => "Beco&#241;a"
            ]
            8 => array:2 [
              "nombre" => "Jos&#233;"
              "apellidos" => "Precioso"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511514001237?idApp=UINPBA00004E"
    "url" => "/21735115/0000002100000004/v1_201507020213/S2173511514001237/v1_201507020213/en/main.assets"
  ]
  "en" => array:18 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>"
    "titulo" => "Diagnostic yield of sputum microbiological analysis in the diagnosis of pulmonary tuberculosis in a period of 10 years"
    "tieneTextoCompleto" => true
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "185"
        "paginaFinal" => "191"
      ]
    ]
    "autores" => array:1 [
      0 => array:4 [
        "autoresLista" => "A&#46; Tavares e Castro, M&#46; Mendes, S&#46; Freitas, P&#46;C&#46; Roxo"
        "autores" => array:4 [
          0 => array:4 [
            "nombre" => "A&#46; Tavares e"
            "apellidos" => "Castro"
            "email" => array:1 [
              0 => "anatavaresecastro&#64;gmail&#46;com"
            ]
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "cor0005"
              ]
            ]
          ]
          1 => array:3 [
            "nombre" => "M&#46;"
            "apellidos" => "Mendes"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff0010"
              ]
            ]
          ]
          2 => array:3 [
            "nombre" => "S&#46;"
            "apellidos" => "Freitas"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
            ]
          ]
          3 => array:3 [
            "nombre" => "P&#46;C&#46;"
            "apellidos" => "Roxo"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">c</span>"
                "identificador" => "aff0015"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:3 [
          0 => array:3 [
            "entidad" => "Pulmonology Unit&#44; Hospitais da Universidade de Coimbra &#8211; Centro Hospitalar e Universit&#225;rio de Coimbra&#44; Coimbra&#44; Portugal"
            "etiqueta" => "a"
            "identificador" => "aff0005"
          ]
          1 => array:3 [
            "entidad" => "Pulmonology Unit&#44; Centro Hospitalar Cova da Beira&#44; Covilh&#227;&#44; Portugal"
            "etiqueta" => "b"
            "identificador" => "aff0010"
          ]
          2 => array:3 [
            "entidad" => "Pulmonology Diagnostic Center of Coimbra&#44; Coimbra&#44; Portugal"
            "etiqueta" => "c"
            "identificador" => "aff0015"
          ]
        ]
        "correspondencia" => array:1 [
          0 => array:3 [
            "identificador" => "cor0005"
            "etiqueta" => "&#8270;"
            "correspondencia" => "Corresponding author&#46;"
          ]
        ]
      ]
    ]
    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Tuberculosis &#40;TB&#41; is still associated with a high global burden&#59; there was an estimated 8&#46;6 million new cases and 1&#46;3 million deaths in 2012&#44; most of them occurring in low- and middle-income countries&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">1</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">Early diagnosis and immediate treatment are essential to cure this airborne infectious disease and prevent transmission in the community&#46; A definite diagnosis can only be established if <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> is isolated and identified from respiratory specimens&#44; most frequently expectorated or induced sputum&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">Therefore&#44; when pulmonary TB is suspected&#44; standard guidelines recommend that clinical specimens should be collected and submitted for laboratory testing&#44; such as smear microscopy&#44; culture and nucleic acid amplification to increase the detection rate of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>&#46;<a class="elsevierStyleCrossRefs" href="#bib0140"><span class="elsevierStyleSup">2&#8211;5</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">It has been stated that laboratory analysis should be performed on at least three sputum specimens&#44; collected over consecutive days&#46;<a class="elsevierStyleCrossRefs" href="#bib0160"><span class="elsevierStyleSup">6&#44;7</span></a> However&#44; there has been some controversy attached to this methodology and some authors find examination of multiple specimens excessive in relation to the yield gain and hard to achieve especially in resource-limited settings&#46;<a class="elsevierStyleCrossRefs" href="#bib0170"><span class="elsevierStyleSup">8&#8211;13</span></a></p><p id="par0025" class="elsevierStylePara elsevierViewall">Culture identification is still the gold standard for diagnosis of pulmonary TB despite the fact that <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> is a slow growing organism and solid medium culture may take up to 4&#8211;8 weeks to provide a positive result&#46; To overcome this limitation&#44; liquid medium culture has emerged as a more sensitive and speedier technique to detect bacilli growth and simultaneously test for drug susceptibility&#46;<a class="elsevierStyleCrossRefs" href="#bib0200"><span class="elsevierStyleSup">14&#8211;16</span></a></p><p id="par0030" class="elsevierStylePara elsevierViewall">In developing countries&#44; sputum smear microscopy is the most commonly used diagnostic test for pulmonary TB&#46; It is a simple technique that quickly identifies acid-fast bacilli &#40;AFB&#41; with a relatively low cost and high positive predictive value&#44; especially when concentrated samples are used&#46; It is important as a tool not only to establish a presumptive diagnosis of pulmonary TB but also to monitor the patient response to anti-TB treatment&#46; In 2009&#44; the WHO recommended that the conventional bright field microscopy using Ziehl&#8211;Neelsen &#40;ZN&#41; stain should be replaced by the more sensitive fluorescent light-emitting diode &#40;LED&#41; microscopy&#44; but up to 2012 only two percent of the microscopy centers had this kind of equipment&#46;<a class="elsevierStyleCrossRefs" href="#bib0135"><span class="elsevierStyleSup">1&#44;17</span></a> Nowadays&#44; many countries&#44; including Portugal&#44; use fluorescent microscopy for sputum microscopy on a routine basis&#46;</p><p id="par0035" class="elsevierStylePara elsevierViewall">Rapid molecular tests are recent diagnostic instruments that can be used to simultaneously test for pulmonary TB and rifampicin resistance with higher sensitivity than sputum smear microscopy and which could replace conventional culture-based drug susceptibility testing&#46;<a class="elsevierStyleCrossRef" href="#bib0220"><span class="elsevierStyleSup">18</span></a> However&#44; these groundbreaking tests are more expensive&#44; require appropriately equipped laboratory settings which are still unavailable in many high-prevalence countries and demand a change in the diagnosis paradigm&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">Thus&#44; at the present time&#44; ZN smear microscopy and solid or liquid medium cultures remain the diagnostic methods most widely used in the diagnosis of TB&#46; The aim of this study was to analyze the sensitivities of these conventional methods in a Portuguese public health department&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Materials and methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Patient selection</span><p id="par0045" class="elsevierStylePara elsevierViewall">Between 2004 and 2013&#44; data from all patients submitted to sputum analysis at the Pulmonology Diagnostic Centre &#40;PDC&#41; laboratory was retrospectively assessed&#46; Only those that had three adequate samples collected in consecutive mornings were included in this study&#46; Non-pulmonary and&#47;or pleural tuberculosis&#44; latent tuberculosis infection &#40;LTBI&#41; and presumed TB based only on clinical grounds were excluded&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">Patients were diagnosed with LTBI when they exhibited no symptoms or signs of the disease except for a positive tuberculin skin test and&#47;or a positive interferon-&#947; release assays &#40;IGRA&#41; test&#46; A diagnosis of tuberculosis was made if <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> was isolated from respiratory samples or if a histological diagnosis was made&#46; Pulmonary TB diagnosis was excluded when all standard diagnostic procedures&#44; including bacteriologic examination&#44; chest radiograph and occasionally histologic analysis&#44; did not confirm active disease suspicion and also&#44; when during follow-up appointments&#44; there was clinical improvement or establishment of an alternative diagnosis&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Microbiology technique</span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">Sputum collection</span><p id="par0055" class="elsevierStylePara elsevierViewall">All patients received prior oral and written specific instructions on the correct sputum collection technique&#46; Collection was done at home immediately after waking up and washing the mouth with water&#44; avoiding toothpaste or antiseptic solutions&#46; Samples were collected to a sterile container three days in a row&#44; placed inside a thermal bag at low temperature in the fridge&#44; avoiding overgrowth of other bacteria&#44; and delivered to the laboratory in the fourth day&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0060">Digestion and decontamination procedure</span><p id="par0060" class="elsevierStylePara elsevierViewall">To maximize the mycobacterial yield&#44; contaminated specimens were submitted to a digestion and decontamination procedure with the N-acetyl-<span class="elsevierStyleSmallCaps">l</span>-cysteine-sodium hydroxide &#40;NALC-NaOH&#41; method &#40;BBL<span class="elsevierStyleSup">&#174;</span> MycoPrep<span class="elsevierStyleSup">TM</span> Specimen Digestion and Decontamination Kit&#41; prior to culture analysis&#46; NALC-NaOH solution was prepared onsite and immediately added to an equal amount of sputum sample &#40;of 5<span class="elsevierStyleHsp" style=""></span>mL each&#41; in a 50<span class="elsevierStyleHsp" style=""></span>mL centrifuge tube due to the rapid loss of NAC activity when the compound is in solution&#46; The decontamination process included adding a phosphate buffer of 6&#46;8 to the 50<span class="elsevierStyleHsp" style=""></span>mL mark before proceeding to centrifugation&#46; The supernatant fluid was carefully decanted and a small amount of the same buffer added to the sediment&#46; The sediment was resuspended and the suspension used for preparation of concentrated smears and the performance of mycobacterial procedures&#46;</p></span></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Microscopy staining technique</span><p id="par0065" class="elsevierStylePara elsevierViewall">Ziehl&#8211;Neelsen stain &#40;BD&#8482; TB Stain Kit ZN&#41; was used to stain two smears prepared from each sputum sample&#44; one direct &#40;unconcentrated&#41; and one concentrated&#44; according to the Ziehl&#8211;Neelsen &#40;hot&#41; acid-fast procedure for early presumptive diagnosis of mycobacterial infection&#46;</p><p id="par0070" class="elsevierStylePara elsevierViewall">The concentrated smear was obtained after a digestion and decontamination procedure with N-acetyl-<span class="elsevierStyleSmallCaps">l</span>-cysteine and sodium hydroxide 2&#37; &#40;BD BBL&#8482; MycoPrep&#8482;&#41; to maximize the mycobacterial yield&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">Smears made from direct and concentrated sputum were observed on optic microscope &#40;Leitz Laborlux K&#41; with oil immersion lens&#46;</p><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Mycobacterial culture</span><p id="par0080" class="elsevierStylePara elsevierViewall">Digested and decontaminated sputum samples were inoculated into a mycobacteria growth indicator tube &#40;MGIT&#8482;&#41; with modified Middlebrook 7H9 broth &#40;BBL&#8482; MGIT&#8482;&#41;&#44; enrichment growth supplement &#40;BBL&#8482; OADC&#41; and an antibiotic mixture against Gram-negative and Gram-positive bacteria and fungi &#40;BBL&#8482; MGIT&#8482; PANTA&#8482;&#41; for the detection and recovery of mycobacteria using the BACTEC&#8482; MGIT&#8482; 960 System&#46; This incubator executes continuous monitoring until there is a positive end to the testing protocol&#46; An instrument positive tube contains approximately 10<span class="elsevierStyleSup">5</span>&#8211;10<span class="elsevierStyleSup">6</span> colony-forming units per milliliter &#40;CFU&#47;mL&#41;&#44; detected by fluorescence analysis&#46; Positive cultures were confirmed by smear microscope analysis&#46; Culture that remained negative up to 45 days and showed no visible signs of positivity were removed from the instrument as negatives&#46;</p><p id="par0085" class="elsevierStylePara elsevierViewall">Samples were also incubated into a solid egg-based medium &#40;BBL&#8482; L&#246;wenstein&#8211;Jensen Medium&#41; for isolation and cultivation of mycobacteria using a Jouan Incubator at 35<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2<span class="elsevierStyleHsp" style=""></span>&#176;C up to 8 weeks&#44; beyond which the result is considered negative&#46;</p></span></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Species identification</span><p id="par0090" class="elsevierStylePara elsevierViewall">A DNA probe test for in <span class="elsevierStyleItalic">vitro</span> routine &#40;INNO-LiPA MYCOBACTERYA v2 assay&#41; was used for the detection and identification of the genus <span class="elsevierStyleItalic">Mycobaterium</span> and 16 different mycobacterial species from solid culture or&#44; if negative&#44; from liquid culture&#46; This assay is based on nucleotide differences in the 16S&#8211;23S ribosomal &#40;rRNA&#41; spacer region&#46;</p></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Antimycobacterial susceptibility testing</span><p id="par0095" class="elsevierStylePara elsevierViewall">Streptomycin&#44; isoniazid&#44; rifampicin and ethambutol susceptibility testing for <span class="elsevierStyleItalic">Mycobaterium tuberculosis</span> were performed according to the Method of Proportion &#40;MOP&#41; procedure with a rapid qualitative procedure &#40;BACTEC&#8482; MGIT&#8482; 960 SIRE Kit&#41;&#44; used with a semi-automated system &#40;BACTEC&#8482; MGIT&#8482; 960 System&#41;&#46; An isolate was determined resistant if one or more of the test population grew in the presence of the critical concentration of the drug&#46; The testing for pyrazinamide requires some modification from the general methods because the drug is active in vitro only at lower pH values&#46; So&#44; pyrazinamide susceptibility testing based on the same principles but in this case a different qualitative procedure was used with a non-radiometric method &#40;BACTEC&#8482; MGIT&#8482; 960 PZA Kit&#41;&#46;</p></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Statistical analysis</span><p id="par0100" class="elsevierStylePara elsevierViewall">Data analysis was executed by IBM SPSS Statistics<span class="elsevierStyleSup">&#174;</span> for Windows version 20&#46;0&#46; All patient demographic and clinical features were reported using frequency and descriptive analyses&#46; Sensitivity&#44; specificity&#44; positive and negative predictive measures were calculated for each and all sputum sample analysis&#46;</p></span></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Results</span><p id="par0105" class="elsevierStylePara elsevierViewall">A total of 753 patients were included in the present study&#46; Thirty-one patients were excluded as 10 of these had latent tuberculosis infection&#44; 18 had extrapulmonary tuberculosis and three were diagnosed based on clinical grounds alone&#44; with no microbiological or histological confirmation&#46; Demographic and clinical characteristics of the remaining 694 patients are categorized in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#46; Mean &#40;range&#41; age was 48&#46;5<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>18&#46;6 &#40;17&#46;1&#8211;87&#46;4&#41; years old&#46; There was a non-significant predominance of male gender and a vast prevalence of Portuguese patients&#46; A minor cluster was foreign-born&#44; from geographical areas such as Portuguese-speaking African countries&#44; East Europe&#44; South America and Middle East&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0110" class="elsevierStylePara elsevierViewall">The underlying criteria for the investigation of tuberculosis among this population were mostly clinical suspicion of tuberculosis based on symptoms and&#47;or radiological changes&#46; A small number of patients were diagnosed while being screened for tuberculosis contacts or while submitted to screening protocols&#44; such as those aimed at rheumatoid candidates for biological treatment&#46;</p><p id="par0115" class="elsevierStylePara elsevierViewall">From all 694 patients&#44; 285 &#40;41&#37;&#41; were diagnosed with active respiratory tuberculosis and 409 &#40;59&#37;&#41; had non-confirmed respiratory tuberculosis&#46; Amongst the latter group of patients&#44; 17 &#40;2&#37;&#41; had other mycobacteria isolated from sputum samples&#44; within which the most frequent was <span class="elsevierStyleItalic">Mycobacterium avium</span>&#46;</p><p id="par0120" class="elsevierStylePara elsevierViewall">Clinical characteristics of patients diagnosed with respiratory tuberculosis are detailed in <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#46; Tuberculosis was diagnosed from sputum microbiological analysis in 180 &#40;63&#37;&#41; patients&#46; In 89 &#40;31&#37;&#41; patients the disease was confirmed by the identification of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> in other respiratory specimens&#44; such as bronchial aspirate or bronchoalveolar lavage&#46; A total of 16 &#40;6&#37;&#41; symptomatic patients had tuberculosis diagnosed by histological analysis of lung biopsy samples obtained through surgical procedures and sustained by a positive IGRA test&#46; Histological examination with hematoxylin and eosin staining revealed in all of these biopsies granulomas with central caseous necrosis ringed by epithelioid macrophages or granulomas with Langhan&#39;s giant cells along with lymphocytes&#44; plasma cells and occasional neutrophils&#46; Additionally&#44; in all these cases&#44; an acid-fast stain with Ziehl&#8211;Neelsen disclosed microorganisms as slender red rods&#46;</p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><p id="par0125" class="elsevierStylePara elsevierViewall">Pulmonary tuberculosis was associated with chest radiographic changes in 88&#37; of the patients&#46; The most frequent radiographic pattern was non-cavitated alterations &#40;54&#37;&#41; but a significant number of patients exhibited cavitated lesions &#40;34&#37;&#41;&#46;</p><p id="par0130" class="elsevierStylePara elsevierViewall">At least one comorbid condition occurred in 99 patients &#40;35&#37;&#41; with tuberculosis&#46; The remaining 186 patients &#40;65&#37;&#41; were otherwise healthy&#46; Diabetes <span class="elsevierStyleItalic">mellitus</span> and cancer were the most frequent underlying diseases&#46; Alcoholism and intravenous drug abuse were also not frequent&#59; 24 and 12 patients respectively were identified&#46;</p><p id="par0135" class="elsevierStylePara elsevierViewall">Twenty-six patients &#40;9&#37;&#41; were considered at risk for tuberculosis due to their residence status&#44; either because they were in prison&#44; living in community residences or were homeless&#46;</p><p id="par0140" class="elsevierStylePara elsevierViewall">Each patient admitted to this study presented three satisfactory sputum samples for in-laboratory microbiological analysis&#44; for a total of 2082 specimens&#46; The diagnostic yield of all the microbiological techniques performed in the sputum samples is stated in <a class="elsevierStyleCrossRef" href="#tbl0015">Table 3</a>&#46; In terms of sputum smear microscopy&#44; the cumulative sensitivity values for each of the three smears were 19&#46;3&#37;&#44; 20&#46;4&#37; and 22&#46;5&#37; for direct smears and 24&#46;6&#37;&#44; 27&#46;7&#37; and 28&#46;8&#37; for concentrated samples&#46; Regarding mycobacterial culture&#44; when only one specimen was tested&#44; the sensitivities of L&#246;wenstein&#8211;Jensen and MGIT medium incubation were 33&#46;3&#37; and 43&#46;9&#37;&#46; When two samples were submitted the cumulative sensitivities increased to 37&#46;9&#37; and 51&#46;6&#37;&#44; and when three were submitted sensitivities were 41&#46;8&#37; and 55&#46;4&#37;&#46; Considering all forms of microbiological sputum analysis &#40;direct and concentrated smear&#44; solid and liquid medium culture&#41;&#44; the sensitivities for the first specimen were 51&#46;2&#37;&#46; The overall cumulative sensitivities values for the second and third sample were 59&#46;6&#37; and 63&#46;2&#37;&#46;</p><elsevierMultimedia ident="tbl0015"></elsevierMultimedia><p id="par0145" class="elsevierStylePara elsevierViewall">The same statistical analysis was only performed for patients with pulmonary tuberculosis and chest radiography with at least one cavitated lesion&#46; Compared to patients with other radiological patterns&#44; patients with cavitation had higher sensitivity values for all microbiological techniques executed&#46; The greatest sensitivity increments were observed for direct and concentrated sputum smear analysis&#44; with an average of 13&#46;0&#37; and 12&#46;4&#37; increase in sensitivity rates&#44; respectively&#46; Regarding cultural analysis&#44; the sensitivity increments were considerably lower with an average of 1&#46;1&#37; increase for L&#246;wenstein&#8211;Jensen and a 5&#46;3&#37; increase for MGIT medium incubation&#46; In this subset of patients&#44; cumulative sensitivities were also higher for all three consecutive sputum sample analysis &#40;58&#46;6&#37;&#44; 63&#46;6&#37; and 67&#46;7&#37;&#41;&#46;</p><p id="par0150" class="elsevierStylePara elsevierViewall">There was a significant increment of sensitivity rates due to additional second and third sputum specimens&#46; Sensitivity differences showed that&#44; when adding a second specimen&#44; the increment increase for direct and concentrated smear&#44; solid and liquid medium culture was 1&#46;1&#37; and 3&#46;2&#37;&#44; 4&#46;6&#37; and 7&#46;7&#37;&#44; respectively&#46; Similarly&#44; when adding a third specimen&#44; the sensitivity increase was 2&#46;1&#37; and 1&#46;1&#37;&#44; 3&#46;9&#37; and 3&#46;9&#37;&#46; The overall sensitivity difference for the two-specimen strategy was 8&#46;4&#37; and for the third-specimen strategy was 3&#46;5&#37;&#46; For patients with cavitated lesions&#44; cumulative sensitivity rates were 5&#46;1&#37; higher when adding a second&#44; and 4&#46;0&#37; higher when adding a third sputum sample&#46;</p></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Discussion</span><p id="par0155" class="elsevierStylePara elsevierViewall">The microscopic staining technique is one of the earliest methods used for detecting <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> and it remains a standard procedure for the diagnosis of tuberculosis because it is swift&#44; cheap and has a high positive predictive value&#46; On the other-hand&#44; microbiological identification of the bacilli by culture remains the <span class="elsevierStyleItalic">gold-standard</span> for the diagnosis of tuberculosis&#46;</p><p id="par0160" class="elsevierStylePara elsevierViewall">Regarding conventional microscopy&#44; the examination of concentrated sputum offered higher diagnostic yield when compared with direct smear&#46; Also&#44; there was an increase in the sensitivity values when considering two or three sputum samples rather than just one&#46; However&#44; the greatest gain was obtained when adding a second sputum sample&#44; while the third sputum sample offered a less substantial sensitivity increment&#46;</p><p id="par0165" class="elsevierStylePara elsevierViewall">Our results agree with the most consensual documented studies that show an incremental yield on more than one sputum examination<a class="elsevierStyleCrossRefs" href="#bib0225"><span class="elsevierStyleSup">19&#8211;21</span></a> and with more recent and controversial small trials claiming the relative inefficiency of the third smear and the burden it carries in undeveloped areas&#46;<a class="elsevierStyleCrossRefs" href="#bib0175"><span class="elsevierStyleSup">9&#8211;13</span></a></p><p id="par0170" class="elsevierStylePara elsevierViewall">The overall sensitivity of sputum smear microscopy&#44; culture analysis and all forms of microbiological analysis together remained low and did not exceed 28&#46;8&#37;&#44; 55&#46;4&#37; and 63&#46;2&#37;&#46; As predicted&#44; higher sensitivity rates were achieved among tuberculosis patients with pulmonary cavitation&#44; ranging from 58&#46;6&#37; for the first specimen to 67&#46;7&#37; for the third specimen&#46;</p><p id="par0175" class="elsevierStylePara elsevierViewall">Low sensitivity rates may be due to pauci-bacillary disease where only a small number of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> organisms are present in the respiratory specimens&#44; and&#47;or to technical problems&#46; During the actual decontamination and concentration process there is tuberculosis bacilli damage that can decrease the sensitivity rates of tuberculosis detection&#44; particularly in pauci-bacillary specimens&#46;</p><p id="par0180" class="elsevierStylePara elsevierViewall">One other possible explanation for a low count of bacilli disease in the tuberculosis patients observed in this study is related to the particular features of the patients observed in the PDC&#46; In fact&#44; the overwhelming majority was highly symptomatic and many had previously gone to their general physician or the hospital&#44; where they had been diagnosed with pneumonia and medicated with antibiotics such as quinolones&#46;</p><p id="par0185" class="elsevierStylePara elsevierViewall">There are also several other technical problems that could be considered&#46; First&#44; the specimen collection was done at home without any kind of medical surveillance&#46; Second&#44; all three samples were delivered to the Laboratory on the third day and only the third sample was submitted for microbiological analysis less than 24<span class="elsevierStyleHsp" style=""></span>h after being collected&#46; Third&#44; specific recommendations were made to each patient to preserve each sample in their domestic fridge&#44; but again there are no guaranties that this was in fact adhered to&#46; All of these methodological issues are directly related to the constraints of the population served by the PDC&#46; In fact&#44; many live far away and cannot afford to pay for three consecutive extra medical visits to provide adequate sputum samples&#46; This strategy was adopted once patients started failing their medical appointments and active tuberculosis cases were not being diagnosed&#46;</p><p id="par0190" class="elsevierStylePara elsevierViewall">To overcome these real-world limitations some alternative strategies could be considered&#46; Several studies have confirmed that the third examination is needless since two sputum smears are as effective as three smears for diagnosing tuberculosis<a class="elsevierStyleCrossRefs" href="#bib0225"><span class="elsevierStyleSup">19&#44;21&#8211;25</span></a> and this has correspondingly been included in the current World Health Organization recommendations on sputum examination&#46; In fact&#44; in this study&#44; the overall diagnostic sensitivity was incremented in 8&#46;4&#37; with two-specimen examination but the third-specimen added only 3&#46;5&#37; to sensitivity rates&#46; Furthermore&#44; a recent systematic review and meta-analysis showed that same-day smear microscopy is as accurate as standard microscopy stategy&#46;<a class="elsevierStyleCrossRef" href="#bib0260"><span class="elsevierStyleSup">26</span></a> So&#44; a possible alternative to standard strategies could be a same-day two-specimen examination&#44; which would diminish technical issues and reduce the number of clinical visits&#46;</p><p id="par0195" class="elsevierStylePara elsevierViewall">An additional methodological limitation needs to be addressed&#46; Sputum microscopy was performed with traditional ZN microscopy but fluorescent LED microscopy could have provided higher sensitivity rates&#46; However&#44; and despite the fact that the WHO recommends a switch to this more sensitive procedure&#44; this technique was not available at the PDC&#46;</p><p id="par0200" class="elsevierStylePara elsevierViewall">When sputum microscopy and culture are both negative&#44; clinical diagnosis of active tuberculosis may have to rely in other conventional diagnostic methods while novel diagnostic tools and further investigation may be required to enhance the identification of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> from biological specimens&#46;</p></span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Conflicts of interest</span><p id="par0205" class="elsevierStylePara elsevierViewall">The authors have no conflicts of interest to declare&#46;</p></span><span id="sec0075" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Ethical disclosures</span><span id="sec0080" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0110">Protection of human and animal subjects</span><p id="par0210" class="elsevierStylePara elsevierViewall">The authors declare that no experiments were performed on humans or animals for this study&#46;</p></span><span id="sec0085" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0115">Confidentiality of data</span><p id="par0215" class="elsevierStylePara elsevierViewall">The authors declare that they have followed the protocols of their work center on the publication of patient data&#46;</p></span><span id="sec0090" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0120">Right to privacy and informed consent</span><p id="par0220" class="elsevierStylePara elsevierViewall">The authors declare that no patient data appear in this article&#46;</p></span></span></span>"
    "textoCompletoSecciones" => array:1 [
      "secciones" => array:9 [
        0 => array:3 [
          "identificador" => "xres528853"
          "titulo" => "Abstract"
          "secciones" => array:4 [
            0 => array:2 [
              "identificador" => "abst0005"
              "titulo" => "Introduction"
            ]
            1 => array:2 [
              "identificador" => "abst0010"
              "titulo" => "Materials and methods"
            ]
            2 => array:2 [
              "identificador" => "abst0015"
              "titulo" => "Results"
            ]
            3 => array:2 [
              "identificador" => "abst0020"
              "titulo" => "Conclusions"
            ]
          ]
        ]
        1 => array:2 [
          "identificador" => "xpalclavsec549084"
          "titulo" => "Keywords"
        ]
        2 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Introduction"
        ]
        3 => array:3 [
          "identificador" => "sec0010"
          "titulo" => "Materials and methods"
          "secciones" => array:6 [
            0 => array:2 [
              "identificador" => "sec0015"
              "titulo" => "Patient selection"
            ]
            1 => array:3 [
              "identificador" => "sec0020"
              "titulo" => "Microbiology technique"
              "secciones" => array:2 [
                0 => array:2 [
                  "identificador" => "sec0025"
                  "titulo" => "Sputum collection"
                ]
                1 => array:2 [
                  "identificador" => "sec0030"
                  "titulo" => "Digestion and decontamination procedure"
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "sec0035"
              "titulo" => "Microscopy staining technique"
              "secciones" => array:1 [
                0 => array:2 [
                  "identificador" => "sec0040"
                  "titulo" => "Mycobacterial culture"
                ]
              ]
            ]
            3 => array:2 [
              "identificador" => "sec0045"
              "titulo" => "Species identification"
            ]
            4 => array:2 [
              "identificador" => "sec0050"
              "titulo" => "Antimycobacterial susceptibility testing"
            ]
            5 => array:2 [
              "identificador" => "sec0055"
              "titulo" => "Statistical analysis"
            ]
          ]
        ]
        4 => array:2 [
          "identificador" => "sec0060"
          "titulo" => "Results"
        ]
        5 => array:2 [
          "identificador" => "sec0065"
          "titulo" => "Discussion"
        ]
        6 => array:2 [
          "identificador" => "sec0070"
          "titulo" => "Conflicts of interest"
        ]
        7 => array:3 [
          "identificador" => "sec0075"
          "titulo" => "Ethical disclosures"
          "secciones" => array:3 [
            0 => array:2 [
              "identificador" => "sec0080"
              "titulo" => "Protection of human and animal subjects"
            ]
            1 => array:2 [
              "identificador" => "sec0085"
              "titulo" => "Confidentiality of data"
            ]
            2 => array:2 [
              "identificador" => "sec0090"
              "titulo" => "Right to privacy and informed consent"
            ]
          ]
        ]
        8 => array:1 [
          "titulo" => "References"
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2014-05-05"
    "fechaAceptado" => "2014-09-05"
    "PalabrasClave" => array:1 [
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec549084"
          "palabras" => array:5 [
            0 => "Tuberculosis"
            1 => "Sputum"
            2 => "Microscopy"
            3 => "Laboratory"
            4 => "<span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:1 [
      "en" => array:3 [
        "titulo" => "Abstract"
        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Introduction</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Pulmonary tuberculosis &#40;TB&#41; requires an early diagnosis for prompt introduction of treatment and prevention of transmission&#46; Definitive diagnosis is obtained by microbiological culture and identification of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> in respiratory specimens&#44; mostly sputum samples&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Materials and methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Retrospective data analysis of all patients suspected of pulmonary TB that submitted three consecutive sputum samples to the Pulmonology Diagnostic Center &#40;PDC&#41; Laboratory between 2004 and 2013&#46; Extrapulmonary TB cases were excluded&#46; Four microbiological analyses were executed on each specimen&#58; two smears with Ziehl&#8211;Neelsen staining&#44; direct and concentrate&#59; and two culture examinations&#44; one in liquid and one in solid medium&#46; Statistical analysis was performed by SPSS&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">A total of 694 patients were enrolled in this study &#40;65&#37; men&#44; mean age 48&#46;5<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>18&#46;6 years&#44; 97&#37; Portuguese&#41;&#44; most of them exhibiting TB-related complaints&#46; Pulmonary TB was diagnosed in 41&#37; of the patients&#59; 54&#37; had non-specific radiological changes and 34&#37; had pulmonary cavitation&#46; The cumulative sensitivity rates of each of the three smears were 24&#46;6&#37;&#44; 27&#46;7&#37; and 28&#46;8&#37; for concentrated samples and 19&#46;3&#37;&#44; 20&#46;4&#37; and 22&#46;5&#37; for direct samples&#46; The cumulative sensitivities of sputum culture were 33&#46;3&#37;&#44; 37&#46;9&#37; and 41&#46;8&#37; for solid medium&#44; and 43&#46;9&#37;&#44; 51&#46;6&#37; and 55&#46;4&#37; for liquid medium&#46; Pondering all forms of microbiological analysis&#44; the cumulative sensitivities of each sample were 51&#46;2&#37;&#44; 59&#46;6&#37; and 63&#46;2&#37;&#46; There was an incremental yield of 8&#46;4&#37; for the second specimen and 3&#46;5&#37; for the third specimen&#46; All sensitivity rates were higher among patients with pulmonary cavitation&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">This study showed an incremental yield with more than one sputum sample&#46; However&#44; overall sensitivity remained low&#44; suggesting a need for new diagnostic strategies and novel and better diagnostic tools&#46;</p></span>"
        "secciones" => array:4 [
          0 => array:2 [
            "identificador" => "abst0005"
            "titulo" => "Introduction"
          ]
          1 => array:2 [
            "identificador" => "abst0010"
            "titulo" => "Materials and methods"
          ]
          2 => array:2 [
            "identificador" => "abst0015"
            "titulo" => "Results"
          ]
          3 => array:2 [
            "identificador" => "abst0020"
            "titulo" => "Conclusions"
          ]
        ]
      ]
    ]
    "NotaPie" => array:1 [
      0 => array:2 [
        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Institution at which work was performed&#58; Centro de Diagn&#243;stico Pneumol&#243;gico de Coimbra&#44; Coimbra&#44; Portugal&#46; Directed by&#58; Paulo Cravo-Roxo&#44; M&#46;D&#46;</p>"
      ]
    ]
    "multimedia" => array:3 [
      0 => array:7 [
        "identificador" => "tbl0005"
        "etiqueta" => "Table 1"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:1 [
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Variable&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">No&#46;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">&#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Age&#44; mean</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">&#177;</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">SD years</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " colspan="2" align="char" valign="top">48&#46;5<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>18&#46;6</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Gender</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Male&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">452&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">65&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Female&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">242&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">35&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Birth origin</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Portugal&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">672&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">97&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>African country of Portuguese-spoken language&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>East Europe&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>South America&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Criteria for tuberculosis suspicion</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Passive screening &#8211; symptoms&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">631&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">91&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Contact screening&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">29&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Protocolized screening&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">27&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Other&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Pulmonary tuberculosis</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>No&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">409&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">59&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">285&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">41&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Sputum mycobacteria</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">180&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">91&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium avium</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium fortuitum</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium gordonae</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium intracellulare</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium kansasii</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium genus</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab852278.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Demographic and clinical characteristics of patients suspected of pulmonary tuberculosis&#46;</p>"
        ]
      ]
      1 => array:7 [
        "identificador" => "tbl0010"
        "etiqueta" => "Table 2"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:1 [
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">No&#46;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">&#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Diagnosis confirmation technique</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Sputum analysis&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">180&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">63&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Bronchial aspirate&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">61&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">21&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Bronchoalveolar lavage&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">28&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Lung biopsy&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">16&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Radiological pattern</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Non-cavitated&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">155&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">54&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Cavitation&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">35&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Normal&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">21&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Unknown&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Comorbidities</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>No comorbidities&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">205&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">72&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Alcoholism&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">24&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Diabetes mellitus&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">18&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Neoplastic disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">14&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Intravenous drug abuse&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">12&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>HIV&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Interstitial pulmonary disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Psychiatric disorder&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary airway disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Chronic liver disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Chronic renal disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Housing status</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Proper house&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">263&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">92&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Social community resident&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">18&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Prison inmate&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Homeless&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab852279.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Clinical characteristics of patients with pulmonary tuberculosis&#46;</p>"
        ]
      ]
      2 => array:7 [
        "identificador" => "tbl0015"
        "etiqueta" => "Table 3"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">MGIT&#44; mycobacteria growth indicator tube&#59; PPV&#44; positive predicted value&#59; NPV&#44; negative predicted value&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">True positive results&#44; <span class="elsevierStyleItalic">n</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">PPV&#44; &#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">NPV&#44; &#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Sensitivity&#44; &#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Specificity&#44; &#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " colspan="6" align="left" valign="top"><span class="elsevierStyleItalic">One specimen</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Direct smear&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">55&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">100&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">64&#46;0&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">19&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">100&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Concentrated smear&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">70&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">97&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">65&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">24&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>L&#246;wenstein&#8211;Jensen medium&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">95&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">96&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">68&#46;1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">33&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>MGIT&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">125&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">93&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">71&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">43&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">97&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Cumulative analysis&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">146&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">93&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">74&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">51&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">97&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="6" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="6" align="left" valign="top"><span class="elsevierStyleItalic">Two specimens</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Direct smear&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">58&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">98&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">64&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">20&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Concentrated smear&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">79&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">95&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">66&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">27&#46;7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&#46;0&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>L&#246;wenstein&#8211;Jensen medium&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">108&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">95&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">69&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">37&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">98&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>MGIT&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">147&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">90&#46;7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">74&#46;1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">51&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">96&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Cumulative analysis&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">170&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">91&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">77&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">59&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">96&#46;1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="6" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="6" align="left" valign="top"><span class="elsevierStyleItalic">Three specimens</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Direct smear&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">64&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">98&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">64&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">22&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Concentrated smear&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">82&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">95&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">66&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">28&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&#46;0&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>L&#246;wenstein&#8211;Jensen medium&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">119&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">95&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">70&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">41&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">98&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>MGIT&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">158&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">90&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">75&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">55&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">96&#46;1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Cumulative analysis&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">180&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">91&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">78&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">63&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">95&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab852277.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Diagnostic yield of sputum analysis submitted to the Pulmonology Diagnostic Centre Laboratory from patients suspected of pulmonary tuberculosis&#46;</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0005"
          "bibliografiaReferencia" => array:26 [
            0 => array:3 [
              "identificador" => "bib0135"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:1 [
                      "titulo" => "WHO &#124; Global tuberculosis report 2013"
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Libro" => array:1 [
                        "fecha" => "2013"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0140"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Large-scale use of polymerase chain reaction for detection of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> in a routine mycobacteriology laboratory"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "J&#46;E&#46; Clarridge"
                            1 => "R&#46;M&#46; Shawar"
                            2 => "T&#46;M&#46; Shinnick"
                            3 => "B&#46;B&#46; Plikaytis"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "1993"
                        "volumen" => "31"
                        "paginaInicial" => "2049"
                        "paginaFinal" => "2056"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8370729"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0145"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Evaluation of sputum smears concentrated by cytocentrifugation for detection of acid-fast bacilli"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "C&#46;A&#46; Saceanu"
                            1 => "N&#46;C&#46; Pfeiffer"
                            2 => "T&#46; McLean"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "1993"
                        "volumen" => "31"
                        "paginaInicial" => "2371"
                        "paginaFinal" => "2374"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8408558"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib0150"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Use of Gen-Probe AccuProbes to identify <span class="elsevierStyleItalic">Mycobacterium avium</span> complex&#44; <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex&#46; <span class="elsevierStyleItalic">Mycobacterium kansasii</span>&#44; and <span class="elsevierStyleItalic">Mycobacterium gordonae</span> directly from BACTEC TB broth cultures"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "B&#46;S&#46; Reisner"
                            1 => "A&#46;M&#46; Gatson"
                            2 => "G&#46;L&#46; Woods"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "1994"
                        "volumen" => "32"
                        "paginaInicial" => "2995"
                        "paginaFinal" => "2998"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7883888"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib0155"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Identification of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> and <span class="elsevierStyleItalic">M&#46; avium</span> complex directly from smear-positive sputum specimens and BACTEC 12B cultures by high-performance liquid chromatography with fluorescence detection and computer-driven pattern recognition models"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "K&#46;C&#46; Jost"
                            1 => "D&#46;F&#46; Dunbar"
                            2 => "S&#46;S&#46; Barth"
                            3 => "V&#46;L&#46; Headley"
                            4 => "L&#46;B&#46; Elliott"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "1995"
                        "volumen" => "33"
                        "paginaInicial" => "1270"
                        "paginaFinal" => "1277"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7615740"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib0160"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:1 [
                      "titulo" => "Manual of clinical microbiology"
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Libro" => array:3 [
                        "fecha" => "1999"
                        "paginaInicial" => "1773"
                        "editorial" => "Amer Society for Microbiology"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib0165"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Diagnostic mycobacteriology laboratory practices"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "T&#46;M&#46; Shinnick"
                            1 => "R&#46;C&#46; Good"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Clin Infect Dis"
                        "fecha" => "1995"
                        "volumen" => "21"
                        "paginaInicial" => "291"
                        "paginaFinal" => "299"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8562734"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib0170"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Tuberculosis control in resource-poor settings with high rates of HIV infection"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "K&#46;M&#46; De Cock"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Public Health"
                        "fecha" => "1996"
                        "volumen" => "86"
                        "paginaInicial" => "1071"
                        "paginaFinal" => "1073"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8712259"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib0175"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The adequacy of one sputum smear for diagnosing pulmonary tuberculosis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "B&#46; Dujardin"
                            1 => "E&#46; Haelterman"
                            2 => "W&#46; Van Damme"
                            3 => "G&#46; Kegels"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Public Health"
                        "fecha" => "1997"
                        "volumen" => "87"
                        "paginaInicial" => "1234"
                        "paginaFinal" => "1235"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9240123"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib0180"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:1 [
                  "referenciaCompleta" => "Ipuge YA&#44; Rieder HL&#44; Enarson DA&#46; The yield of acid-fast bacilli from serial smears in routine microscopy laboratories in rural Tanzania&#46; <span class="elsevierStyleItalic">Trans R Soc Trop Med Hyg</span>&#46; 90&#40;3&#41;&#58;258&#8211;261&#46;"
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib0185"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Diagnostic yield of repeated smear microscopy examinations among patients suspected of pulmonary TB in Shandong province of China"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "Z&#46;L&#46; Wu"
                            1 => "A&#46;Q&#46; Wang"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2000"
                        "volumen" => "4"
                        "paginaInicial" => "1086"
                        "paginaFinal" => "1087"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11092724"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            11 => array:3 [
              "identificador" => "bib0190"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Comparison of two versus three smears in identifying culture-positive tuberculosis patients in a rural African setting with high HIV prevalence"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "A&#46;C&#46; Crampin"
                            1 => "S&#46; Floyd"
                            2 => "F&#46; Mwaungulu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2001"
                        "volumen" => "5"
                        "paginaInicial" => "994"
                        "paginaFinal" => "999"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11716350"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib0195"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Screening tuberculosis suspects using two sputum smears"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "A&#46;D&#46; Harries"
                            1 => "N&#46;B&#46; Mphasa"
                            2 => "C&#46; Mundy"
                            3 => "A&#46; Banerjee"
                            4 => "J&#46;H&#46; Kwanjana"
                            5 => "F&#46;M&#46; Salaniponi"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2000"
                        "volumen" => "4"
                        "paginaInicial" => "36"
                        "paginaFinal" => "40"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10654642"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib0200"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Liquid vs&#46; solid culture for tuberculosis&#58; performance and cost in a resource-constrained setting"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "V&#46;N&#46; Chihota"
                            1 => "A&#46;D&#46; Grant"
                            2 => "K&#46; Fielding"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2010"
                        "volumen" => "14"
                        "paginaInicial" => "1024"
                        "paginaFinal" => "1031"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20626948"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib0205"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Time to detection of the growth of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> in MGIT 960 for determining the early bactericidal activity of antituberculosis agents"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "A&#46;H&#46; Diacon"
                            1 => "J&#46;S&#46; Maritz"
                            2 => "A&#46; Venter"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1007/s10096-010-1043-7"
                      "Revista" => array:6 [
                        "tituloSerie" => "Eur J Clin Microbiol Infect Dis"
                        "fecha" => "2010"
                        "volumen" => "29"
                        "paginaInicial" => "1561"
                        "paginaFinal" => "1565"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20820832"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            15 => array:3 [
              "identificador" => "bib0210"
              "etiqueta" => "16"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Testing of susceptibility of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> to pyrazinamide with the nonradiometric BACTEC MGIT 960 system"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "G&#46;E&#46; Pfyffer"
                            1 => "F&#46; Palicova"
                            2 => "S&#46; R&#252;sch-Gerdes"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "2002"
                        "volumen" => "40"
                        "paginaInicial" => "1670"
                        "paginaFinal" => "1674"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11980940"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            16 => array:3 [
              "identificador" => "bib0215"
              "etiqueta" => "17"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Fluorescence versus conventional sputum smear microscopy for tuberculosis&#58; a systematic review"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "K&#46;R&#46; Steingart"
                            1 => "M&#46; Henry"
                            2 => "V&#46; Ng"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/S1473-3099(06)70578-3"
                      "Revista" => array:6 [
                        "tituloSerie" => "Lancet Infect Dis"
                        "fecha" => "2006"
                        "volumen" => "6"
                        "paginaInicial" => "570"
                        "paginaFinal" => "581"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16931408"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            17 => array:3 [
              "identificador" => "bib0220"
              "etiqueta" => "18"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Xpert<span class="elsevierStyleSup">&#174;</span> MTB&#47;RIF assay for pulmonary tuberculosis and rifampicin resistance in adults"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "K&#46;R&#46; Steingart"
                            1 => "H&#46; Sohn"
                            2 => "I&#46; Schiller"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1002/14651858.CD009593.pub2"
                      "Revista" => array:5 [
                        "tituloSerie" => "Cochrane Database Syst Rev"
                        "fecha" => "2013"
                        "volumen" => "1"
                        "paginaInicial" => "CD009593"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/24448973"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            18 => array:3 [
              "identificador" => "bib0225"
              "etiqueta" => "19"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "A method to determine the utility of the third diagnostic and the second follow-up sputum smear examinations to diagnose tuberculosis cases and failures"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "H&#46;L&#46; Rieder"
                            1 => "C&#46;Y&#46; Chiang"
                            2 => "I&#46;D&#46; Rusen"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2005"
                        "volumen" => "9"
                        "paginaInicial" => "384"
                        "paginaFinal" => "391"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15830743"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            19 => array:3 [
              "identificador" => "bib0230"
              "etiqueta" => "20"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Value of examining multiple sputum specimens in the diagnosis of pulmonary tuberculosis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "S&#46;M&#46; Nelson"
                            1 => "M&#46;A&#46; Deike"
                            2 => "C&#46;P&#46; Cartwright"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "1998"
                        "volumen" => "36"
                        "paginaInicial" => "467"
                        "paginaFinal" => "469"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9466760"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            20 => array:3 [
              "identificador" => "bib0235"
              "etiqueta" => "21"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Yield of serial sputum specimen examinations in the diagnosis of pulmonary tuberculosis&#58; a systematic review"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "S&#46;R&#46; Mase"
                            1 => "A&#46; Ramsay"
                            2 => "V&#46; Ng"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2007"
                        "volumen" => "11"
                        "paginaInicial" => "485"
                        "paginaFinal" => "495"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17439669"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            21 => array:3 [
              "identificador" => "bib0240"
              "etiqueta" => "22"
              "referencia" => array:1 [
                0 => array:1 [
                  "referenciaCompleta" => "Kumar A&#44; Bairy I&#44; Rao CR&#44; Prasad V&#44; Pattanshetty S&#44; Samarasinghe CM&#46; Adequacy of two versus three sputum specimens for the diagnosis of pulmonary tuberculosis&#46; <span class="elsevierStyleItalic">Natl Med J India</span>&#46; 22&#40;6&#41;&#58;298&#8211;299&#46;"
                ]
              ]
            ]
            22 => array:3 [
              "identificador" => "bib0245"
              "etiqueta" => "23"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Yield of two consecutive sputum specimens for the effective diagnosis of pulmonary tuberculosis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "M&#46;R&#46; Islam"
                            1 => "R&#46; Khatun"
                            2 => "M&#46;K&#46;M&#46; Uddin"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1371/journal.pone.0067678"
                      "Revista" => array:5 [
                        "tituloSerie" => "PLOS ONE"
                        "fecha" => "2013"
                        "volumen" => "8"
                        "paginaInicial" => "e67678"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23844057"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            23 => array:3 [
              "identificador" => "bib0250"
              "etiqueta" => "24"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Reducing the number of sputum samples examined and thresholds for positivity&#58; an opportunity to optimise smear microscopy"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "M&#46; Bonnet"
                            1 => "A&#46; Ramsay"
                            2 => "L&#46; Gagnidze"
                            3 => "W&#46; Githui"
                            4 => "P&#46;J&#46; Guerin"
                            5 => "F&#46; Varaine"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2007"
                        "volumen" => "11"
                        "paginaInicial" => "953"
                        "paginaFinal" => "958"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17705971"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            24 => array:3 [
              "identificador" => "bib0255"
              "etiqueta" => "25"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Efficiency of a third serial sputum smear examination in the diagnosis of tuberculosis in Moldova and Uganda"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "A&#46; Katamba"
                            1 => "D&#46; Laticevschi"
                            2 => "H&#46;L&#46; Rieder"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2007"
                        "volumen" => "11"
                        "paginaInicial" => "659"
                        "paginaFinal" => "664"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17519098"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            25 => array:3 [
              "identificador" => "bib0260"
              "etiqueta" => "26"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Diagnostic accuracy of same-day microscopy versus standard microscopy for pulmonary tuberculosis&#58; a systematic review and meta-analysis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "J&#46;L&#46; Davis"
                            1 => "A&#46; Cattamanchi"
                            2 => "L&#46;E&#46; Cuevas"
                            3 => "P&#46;C&#46; Hopewell"
                            4 => "K&#46;R&#46; Steingart"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/S1473-3099(12)70232-3"
                      "Revista" => array:6 [
                        "tituloSerie" => "Lancet Infect Dis"
                        "fecha" => "2013"
                        "volumen" => "13"
                        "paginaInicial" => "147"
                        "paginaFinal" => "154"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23099183"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/21735115/0000002100000004/v1_201507020213/S2173511515000391/v1_201507020213/en/main.assets"
  "Apartado" => array:4 [
    "identificador" => "9710"
    "tipo" => "SECCION"
    "en" => array:2 [
      "titulo" => "Original articles"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "en"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/21735115/0000002100000004/v1_201507020213/S2173511515000391/v1_201507020213/en/main.pdf?idApp=UINPBA00004E&text.app=https://journalpulmonology.org/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511515000391?idApp=UINPBA00004E"
]
Share
Journal Information

Statistics

Follow this link to access the full text of the article

Original article
Diagnostic yield of sputum microbiological analysis in the diagnosis of pulmonary tuberculosis in a period of 10 years
A. Tavares e Castroa,
Corresponding author
anatavaresecastro@gmail.com

Corresponding author.
, M. Mendesb, S. Freitasa, P.C. Roxoc
a Pulmonology Unit, Hospitais da Universidade de Coimbra – Centro Hospitalar e Universitário de Coimbra, Coimbra, Portugal
b Pulmonology Unit, Centro Hospitalar Cova da Beira, Covilhã, Portugal
c Pulmonology Diagnostic Center of Coimbra, Coimbra, Portugal
Read
1285
Times
was read the article
567
Total PDF
718
Total HTML
Share statistics
 array:24 [
  "pii" => "S2173511515000391"
  "issn" => "21735115"
  "doi" => "10.1016/j.rppnen.2014.09.010"
  "estado" => "S300"
  "fechaPublicacion" => "2015-07-01"
  "aid" => "1040"
  "copyright" => "Sociedade Portuguesa de Pneumologia"
  "copyrightAnyo" => "2014"
  "documento" => "article"
  "crossmark" => 1
  "licencia" => "http://www.elsevier.com/open-access/userlicense/1.0/"
  "subdocumento" => "fla"
  "cita" => "Rev Port Pneumol. 2015;21:185-91"
  "abierto" => array:3 [
    "ES" => true
    "ES2" => true
    "LATM" => true
  ]
  "gratuito" => true
  "lecturas" => array:2 [
    "total" => 1528
    "formatos" => array:3 [
      "EPUB" => 209
      "HTML" => 712
      "PDF" => 607
    ]
  ]
  "itemSiguiente" => array:19 [
    "pii" => "S2173511515000172"
    "issn" => "21735115"
    "doi" => "10.1016/j.rppnen.2014.12.003"
    "estado" => "S300"
    "fechaPublicacion" => "2015-07-01"
    "aid" => "1021"
    "copyright" => "Sociedade Portuguesa de Pneumologia"
    "documento" => "article"
    "crossmark" => 1
    "licencia" => "http://www.elsevier.com/open-access/userlicense/1.0/"
    "subdocumento" => "fla"
    "cita" => "Rev Port Pneumol. 2015;21:192-7"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 1503
      "formatos" => array:3 [
        "EPUB" => 207
        "HTML" => 645
        "PDF" => 651
      ]
    ]
    "en" => array:11 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>"
      "titulo" => "Respiratory parameters in elite athletes &#8211; does sport have an influence&#63;"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => "en"
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "192"
          "paginaFinal" => "197"
        ]
      ]
      "contieneResumen" => array:1 [
        "en" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "S&#46; Mazic, B&#46; Lazovic, M&#46; Djelic, J&#46; Suzic-Lazic, S&#46; Djordjevic-Saranovic, T&#46; Durmic, I&#46; Soldatovic, D&#46; Zikic, Z&#46; Gluvic, V&#46; Zugic"
          "autores" => array:10 [
            0 => array:2 [
              "nombre" => "S&#46;"
              "apellidos" => "Mazic"
            ]
            1 => array:2 [
              "nombre" => "B&#46;"
              "apellidos" => "Lazovic"
            ]
            2 => array:2 [
              "nombre" => "M&#46;"
              "apellidos" => "Djelic"
            ]
            3 => array:2 [
              "nombre" => "J&#46;"
              "apellidos" => "Suzic-Lazic"
            ]
            4 => array:2 [
              "nombre" => "S&#46;"
              "apellidos" => "Djordjevic-Saranovic"
            ]
            5 => array:2 [
              "nombre" => "T&#46;"
              "apellidos" => "Durmic"
            ]
            6 => array:2 [
              "nombre" => "I&#46;"
              "apellidos" => "Soldatovic"
            ]
            7 => array:2 [
              "nombre" => "D&#46;"
              "apellidos" => "Zikic"
            ]
            8 => array:2 [
              "nombre" => "Z&#46;"
              "apellidos" => "Gluvic"
            ]
            9 => array:2 [
              "nombre" => "V&#46;"
              "apellidos" => "Zugic"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511515000172?idApp=UINPBA00004E"
    "url" => "/21735115/0000002100000004/v1_201507020213/S2173511515000172/v1_201507020213/en/main.assets"
  ]
  "itemAnterior" => array:19 [
    "pii" => "S2173511514001237"
    "issn" => "21735115"
    "doi" => "10.1016/j.rppnen.2014.09.003"
    "estado" => "S300"
    "fechaPublicacion" => "2015-07-01"
    "aid" => "1003"
    "copyright" => "Sociedade Portuguesa de Pneumologia"
    "documento" => "article"
    "crossmark" => 1
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "fla"
    "cita" => "Rev Port Pneumol. 2015;21:178-84"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 1317
      "formatos" => array:3 [
        "EPUB" => 218
        "HTML" => 597
        "PDF" => 502
      ]
    ]
    "en" => array:11 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>"
      "titulo" => "Children&#39;s exposure to second hand smoke at home&#58; A cross-sectional study in Portugal"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => "en"
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "178"
          "paginaFinal" => "184"
        ]
      ]
      "contieneResumen" => array:1 [
        "en" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Paulo D&#46; Vit&#243;ria, Jos&#233; Cunha Machado, Ana Carolina Ara&#250;jo, Sofia B&#46; Ravara, Catarina Samorinha, Henedina Antunes, Manuel Rosas, Elisardo Beco&#241;a, Jos&#233; Precioso"
          "autores" => array:9 [
            0 => array:2 [
              "nombre" => "Paulo D&#46;"
              "apellidos" => "Vit&#243;ria"
            ]
            1 => array:2 [
              "nombre" => "Jos&#233; Cunha"
              "apellidos" => "Machado"
            ]
            2 => array:2 [
              "nombre" => "Ana Carolina"
              "apellidos" => "Ara&#250;jo"
            ]
            3 => array:2 [
              "nombre" => "Sofia B&#46;"
              "apellidos" => "Ravara"
            ]
            4 => array:2 [
              "nombre" => "Catarina"
              "apellidos" => "Samorinha"
            ]
            5 => array:2 [
              "nombre" => "Henedina"
              "apellidos" => "Antunes"
            ]
            6 => array:2 [
              "nombre" => "Manuel"
              "apellidos" => "Rosas"
            ]
            7 => array:2 [
              "nombre" => "Elisardo"
              "apellidos" => "Beco&#241;a"
            ]
            8 => array:2 [
              "nombre" => "Jos&#233;"
              "apellidos" => "Precioso"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511514001237?idApp=UINPBA00004E"
    "url" => "/21735115/0000002100000004/v1_201507020213/S2173511514001237/v1_201507020213/en/main.assets"
  ]
  "en" => array:18 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>"
    "titulo" => "Diagnostic yield of sputum microbiological analysis in the diagnosis of pulmonary tuberculosis in a period of 10 years"
    "tieneTextoCompleto" => true
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "185"
        "paginaFinal" => "191"
      ]
    ]
    "autores" => array:1 [
      0 => array:4 [
        "autoresLista" => "A&#46; Tavares e Castro, M&#46; Mendes, S&#46; Freitas, P&#46;C&#46; Roxo"
        "autores" => array:4 [
          0 => array:4 [
            "nombre" => "A&#46; Tavares e"
            "apellidos" => "Castro"
            "email" => array:1 [
              0 => "anatavaresecastro&#64;gmail&#46;com"
            ]
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "cor0005"
              ]
            ]
          ]
          1 => array:3 [
            "nombre" => "M&#46;"
            "apellidos" => "Mendes"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff0010"
              ]
            ]
          ]
          2 => array:3 [
            "nombre" => "S&#46;"
            "apellidos" => "Freitas"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
            ]
          ]
          3 => array:3 [
            "nombre" => "P&#46;C&#46;"
            "apellidos" => "Roxo"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">c</span>"
                "identificador" => "aff0015"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:3 [
          0 => array:3 [
            "entidad" => "Pulmonology Unit&#44; Hospitais da Universidade de Coimbra &#8211; Centro Hospitalar e Universit&#225;rio de Coimbra&#44; Coimbra&#44; Portugal"
            "etiqueta" => "a"
            "identificador" => "aff0005"
          ]
          1 => array:3 [
            "entidad" => "Pulmonology Unit&#44; Centro Hospitalar Cova da Beira&#44; Covilh&#227;&#44; Portugal"
            "etiqueta" => "b"
            "identificador" => "aff0010"
          ]
          2 => array:3 [
            "entidad" => "Pulmonology Diagnostic Center of Coimbra&#44; Coimbra&#44; Portugal"
            "etiqueta" => "c"
            "identificador" => "aff0015"
          ]
        ]
        "correspondencia" => array:1 [
          0 => array:3 [
            "identificador" => "cor0005"
            "etiqueta" => "&#8270;"
            "correspondencia" => "Corresponding author&#46;"
          ]
        ]
      ]
    ]
    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Tuberculosis &#40;TB&#41; is still associated with a high global burden&#59; there was an estimated 8&#46;6 million new cases and 1&#46;3 million deaths in 2012&#44; most of them occurring in low- and middle-income countries&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">1</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">Early diagnosis and immediate treatment are essential to cure this airborne infectious disease and prevent transmission in the community&#46; A definite diagnosis can only be established if <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> is isolated and identified from respiratory specimens&#44; most frequently expectorated or induced sputum&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">Therefore&#44; when pulmonary TB is suspected&#44; standard guidelines recommend that clinical specimens should be collected and submitted for laboratory testing&#44; such as smear microscopy&#44; culture and nucleic acid amplification to increase the detection rate of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>&#46;<a class="elsevierStyleCrossRefs" href="#bib0140"><span class="elsevierStyleSup">2&#8211;5</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">It has been stated that laboratory analysis should be performed on at least three sputum specimens&#44; collected over consecutive days&#46;<a class="elsevierStyleCrossRefs" href="#bib0160"><span class="elsevierStyleSup">6&#44;7</span></a> However&#44; there has been some controversy attached to this methodology and some authors find examination of multiple specimens excessive in relation to the yield gain and hard to achieve especially in resource-limited settings&#46;<a class="elsevierStyleCrossRefs" href="#bib0170"><span class="elsevierStyleSup">8&#8211;13</span></a></p><p id="par0025" class="elsevierStylePara elsevierViewall">Culture identification is still the gold standard for diagnosis of pulmonary TB despite the fact that <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> is a slow growing organism and solid medium culture may take up to 4&#8211;8 weeks to provide a positive result&#46; To overcome this limitation&#44; liquid medium culture has emerged as a more sensitive and speedier technique to detect bacilli growth and simultaneously test for drug susceptibility&#46;<a class="elsevierStyleCrossRefs" href="#bib0200"><span class="elsevierStyleSup">14&#8211;16</span></a></p><p id="par0030" class="elsevierStylePara elsevierViewall">In developing countries&#44; sputum smear microscopy is the most commonly used diagnostic test for pulmonary TB&#46; It is a simple technique that quickly identifies acid-fast bacilli &#40;AFB&#41; with a relatively low cost and high positive predictive value&#44; especially when concentrated samples are used&#46; It is important as a tool not only to establish a presumptive diagnosis of pulmonary TB but also to monitor the patient response to anti-TB treatment&#46; In 2009&#44; the WHO recommended that the conventional bright field microscopy using Ziehl&#8211;Neelsen &#40;ZN&#41; stain should be replaced by the more sensitive fluorescent light-emitting diode &#40;LED&#41; microscopy&#44; but up to 2012 only two percent of the microscopy centers had this kind of equipment&#46;<a class="elsevierStyleCrossRefs" href="#bib0135"><span class="elsevierStyleSup">1&#44;17</span></a> Nowadays&#44; many countries&#44; including Portugal&#44; use fluorescent microscopy for sputum microscopy on a routine basis&#46;</p><p id="par0035" class="elsevierStylePara elsevierViewall">Rapid molecular tests are recent diagnostic instruments that can be used to simultaneously test for pulmonary TB and rifampicin resistance with higher sensitivity than sputum smear microscopy and which could replace conventional culture-based drug susceptibility testing&#46;<a class="elsevierStyleCrossRef" href="#bib0220"><span class="elsevierStyleSup">18</span></a> However&#44; these groundbreaking tests are more expensive&#44; require appropriately equipped laboratory settings which are still unavailable in many high-prevalence countries and demand a change in the diagnosis paradigm&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">Thus&#44; at the present time&#44; ZN smear microscopy and solid or liquid medium cultures remain the diagnostic methods most widely used in the diagnosis of TB&#46; The aim of this study was to analyze the sensitivities of these conventional methods in a Portuguese public health department&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Materials and methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Patient selection</span><p id="par0045" class="elsevierStylePara elsevierViewall">Between 2004 and 2013&#44; data from all patients submitted to sputum analysis at the Pulmonology Diagnostic Centre &#40;PDC&#41; laboratory was retrospectively assessed&#46; Only those that had three adequate samples collected in consecutive mornings were included in this study&#46; Non-pulmonary and&#47;or pleural tuberculosis&#44; latent tuberculosis infection &#40;LTBI&#41; and presumed TB based only on clinical grounds were excluded&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">Patients were diagnosed with LTBI when they exhibited no symptoms or signs of the disease except for a positive tuberculin skin test and&#47;or a positive interferon-&#947; release assays &#40;IGRA&#41; test&#46; A diagnosis of tuberculosis was made if <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> was isolated from respiratory samples or if a histological diagnosis was made&#46; Pulmonary TB diagnosis was excluded when all standard diagnostic procedures&#44; including bacteriologic examination&#44; chest radiograph and occasionally histologic analysis&#44; did not confirm active disease suspicion and also&#44; when during follow-up appointments&#44; there was clinical improvement or establishment of an alternative diagnosis&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Microbiology technique</span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">Sputum collection</span><p id="par0055" class="elsevierStylePara elsevierViewall">All patients received prior oral and written specific instructions on the correct sputum collection technique&#46; Collection was done at home immediately after waking up and washing the mouth with water&#44; avoiding toothpaste or antiseptic solutions&#46; Samples were collected to a sterile container three days in a row&#44; placed inside a thermal bag at low temperature in the fridge&#44; avoiding overgrowth of other bacteria&#44; and delivered to the laboratory in the fourth day&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0060">Digestion and decontamination procedure</span><p id="par0060" class="elsevierStylePara elsevierViewall">To maximize the mycobacterial yield&#44; contaminated specimens were submitted to a digestion and decontamination procedure with the N-acetyl-<span class="elsevierStyleSmallCaps">l</span>-cysteine-sodium hydroxide &#40;NALC-NaOH&#41; method &#40;BBL<span class="elsevierStyleSup">&#174;</span> MycoPrep<span class="elsevierStyleSup">TM</span> Specimen Digestion and Decontamination Kit&#41; prior to culture analysis&#46; NALC-NaOH solution was prepared onsite and immediately added to an equal amount of sputum sample &#40;of 5<span class="elsevierStyleHsp" style=""></span>mL each&#41; in a 50<span class="elsevierStyleHsp" style=""></span>mL centrifuge tube due to the rapid loss of NAC activity when the compound is in solution&#46; The decontamination process included adding a phosphate buffer of 6&#46;8 to the 50<span class="elsevierStyleHsp" style=""></span>mL mark before proceeding to centrifugation&#46; The supernatant fluid was carefully decanted and a small amount of the same buffer added to the sediment&#46; The sediment was resuspended and the suspension used for preparation of concentrated smears and the performance of mycobacterial procedures&#46;</p></span></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Microscopy staining technique</span><p id="par0065" class="elsevierStylePara elsevierViewall">Ziehl&#8211;Neelsen stain &#40;BD&#8482; TB Stain Kit ZN&#41; was used to stain two smears prepared from each sputum sample&#44; one direct &#40;unconcentrated&#41; and one concentrated&#44; according to the Ziehl&#8211;Neelsen &#40;hot&#41; acid-fast procedure for early presumptive diagnosis of mycobacterial infection&#46;</p><p id="par0070" class="elsevierStylePara elsevierViewall">The concentrated smear was obtained after a digestion and decontamination procedure with N-acetyl-<span class="elsevierStyleSmallCaps">l</span>-cysteine and sodium hydroxide 2&#37; &#40;BD BBL&#8482; MycoPrep&#8482;&#41; to maximize the mycobacterial yield&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">Smears made from direct and concentrated sputum were observed on optic microscope &#40;Leitz Laborlux K&#41; with oil immersion lens&#46;</p><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Mycobacterial culture</span><p id="par0080" class="elsevierStylePara elsevierViewall">Digested and decontaminated sputum samples were inoculated into a mycobacteria growth indicator tube &#40;MGIT&#8482;&#41; with modified Middlebrook 7H9 broth &#40;BBL&#8482; MGIT&#8482;&#41;&#44; enrichment growth supplement &#40;BBL&#8482; OADC&#41; and an antibiotic mixture against Gram-negative and Gram-positive bacteria and fungi &#40;BBL&#8482; MGIT&#8482; PANTA&#8482;&#41; for the detection and recovery of mycobacteria using the BACTEC&#8482; MGIT&#8482; 960 System&#46; This incubator executes continuous monitoring until there is a positive end to the testing protocol&#46; An instrument positive tube contains approximately 10<span class="elsevierStyleSup">5</span>&#8211;10<span class="elsevierStyleSup">6</span> colony-forming units per milliliter &#40;CFU&#47;mL&#41;&#44; detected by fluorescence analysis&#46; Positive cultures were confirmed by smear microscope analysis&#46; Culture that remained negative up to 45 days and showed no visible signs of positivity were removed from the instrument as negatives&#46;</p><p id="par0085" class="elsevierStylePara elsevierViewall">Samples were also incubated into a solid egg-based medium &#40;BBL&#8482; L&#246;wenstein&#8211;Jensen Medium&#41; for isolation and cultivation of mycobacteria using a Jouan Incubator at 35<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>2<span class="elsevierStyleHsp" style=""></span>&#176;C up to 8 weeks&#44; beyond which the result is considered negative&#46;</p></span></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Species identification</span><p id="par0090" class="elsevierStylePara elsevierViewall">A DNA probe test for in <span class="elsevierStyleItalic">vitro</span> routine &#40;INNO-LiPA MYCOBACTERYA v2 assay&#41; was used for the detection and identification of the genus <span class="elsevierStyleItalic">Mycobaterium</span> and 16 different mycobacterial species from solid culture or&#44; if negative&#44; from liquid culture&#46; This assay is based on nucleotide differences in the 16S&#8211;23S ribosomal &#40;rRNA&#41; spacer region&#46;</p></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Antimycobacterial susceptibility testing</span><p id="par0095" class="elsevierStylePara elsevierViewall">Streptomycin&#44; isoniazid&#44; rifampicin and ethambutol susceptibility testing for <span class="elsevierStyleItalic">Mycobaterium tuberculosis</span> were performed according to the Method of Proportion &#40;MOP&#41; procedure with a rapid qualitative procedure &#40;BACTEC&#8482; MGIT&#8482; 960 SIRE Kit&#41;&#44; used with a semi-automated system &#40;BACTEC&#8482; MGIT&#8482; 960 System&#41;&#46; An isolate was determined resistant if one or more of the test population grew in the presence of the critical concentration of the drug&#46; The testing for pyrazinamide requires some modification from the general methods because the drug is active in vitro only at lower pH values&#46; So&#44; pyrazinamide susceptibility testing based on the same principles but in this case a different qualitative procedure was used with a non-radiometric method &#40;BACTEC&#8482; MGIT&#8482; 960 PZA Kit&#41;&#46;</p></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Statistical analysis</span><p id="par0100" class="elsevierStylePara elsevierViewall">Data analysis was executed by IBM SPSS Statistics<span class="elsevierStyleSup">&#174;</span> for Windows version 20&#46;0&#46; All patient demographic and clinical features were reported using frequency and descriptive analyses&#46; Sensitivity&#44; specificity&#44; positive and negative predictive measures were calculated for each and all sputum sample analysis&#46;</p></span></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Results</span><p id="par0105" class="elsevierStylePara elsevierViewall">A total of 753 patients were included in the present study&#46; Thirty-one patients were excluded as 10 of these had latent tuberculosis infection&#44; 18 had extrapulmonary tuberculosis and three were diagnosed based on clinical grounds alone&#44; with no microbiological or histological confirmation&#46; Demographic and clinical characteristics of the remaining 694 patients are categorized in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#46; Mean &#40;range&#41; age was 48&#46;5<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>18&#46;6 &#40;17&#46;1&#8211;87&#46;4&#41; years old&#46; There was a non-significant predominance of male gender and a vast prevalence of Portuguese patients&#46; A minor cluster was foreign-born&#44; from geographical areas such as Portuguese-speaking African countries&#44; East Europe&#44; South America and Middle East&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0110" class="elsevierStylePara elsevierViewall">The underlying criteria for the investigation of tuberculosis among this population were mostly clinical suspicion of tuberculosis based on symptoms and&#47;or radiological changes&#46; A small number of patients were diagnosed while being screened for tuberculosis contacts or while submitted to screening protocols&#44; such as those aimed at rheumatoid candidates for biological treatment&#46;</p><p id="par0115" class="elsevierStylePara elsevierViewall">From all 694 patients&#44; 285 &#40;41&#37;&#41; were diagnosed with active respiratory tuberculosis and 409 &#40;59&#37;&#41; had non-confirmed respiratory tuberculosis&#46; Amongst the latter group of patients&#44; 17 &#40;2&#37;&#41; had other mycobacteria isolated from sputum samples&#44; within which the most frequent was <span class="elsevierStyleItalic">Mycobacterium avium</span>&#46;</p><p id="par0120" class="elsevierStylePara elsevierViewall">Clinical characteristics of patients diagnosed with respiratory tuberculosis are detailed in <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#46; Tuberculosis was diagnosed from sputum microbiological analysis in 180 &#40;63&#37;&#41; patients&#46; In 89 &#40;31&#37;&#41; patients the disease was confirmed by the identification of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> in other respiratory specimens&#44; such as bronchial aspirate or bronchoalveolar lavage&#46; A total of 16 &#40;6&#37;&#41; symptomatic patients had tuberculosis diagnosed by histological analysis of lung biopsy samples obtained through surgical procedures and sustained by a positive IGRA test&#46; Histological examination with hematoxylin and eosin staining revealed in all of these biopsies granulomas with central caseous necrosis ringed by epithelioid macrophages or granulomas with Langhan&#39;s giant cells along with lymphocytes&#44; plasma cells and occasional neutrophils&#46; Additionally&#44; in all these cases&#44; an acid-fast stain with Ziehl&#8211;Neelsen disclosed microorganisms as slender red rods&#46;</p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><p id="par0125" class="elsevierStylePara elsevierViewall">Pulmonary tuberculosis was associated with chest radiographic changes in 88&#37; of the patients&#46; The most frequent radiographic pattern was non-cavitated alterations &#40;54&#37;&#41; but a significant number of patients exhibited cavitated lesions &#40;34&#37;&#41;&#46;</p><p id="par0130" class="elsevierStylePara elsevierViewall">At least one comorbid condition occurred in 99 patients &#40;35&#37;&#41; with tuberculosis&#46; The remaining 186 patients &#40;65&#37;&#41; were otherwise healthy&#46; Diabetes <span class="elsevierStyleItalic">mellitus</span> and cancer were the most frequent underlying diseases&#46; Alcoholism and intravenous drug abuse were also not frequent&#59; 24 and 12 patients respectively were identified&#46;</p><p id="par0135" class="elsevierStylePara elsevierViewall">Twenty-six patients &#40;9&#37;&#41; were considered at risk for tuberculosis due to their residence status&#44; either because they were in prison&#44; living in community residences or were homeless&#46;</p><p id="par0140" class="elsevierStylePara elsevierViewall">Each patient admitted to this study presented three satisfactory sputum samples for in-laboratory microbiological analysis&#44; for a total of 2082 specimens&#46; The diagnostic yield of all the microbiological techniques performed in the sputum samples is stated in <a class="elsevierStyleCrossRef" href="#tbl0015">Table 3</a>&#46; In terms of sputum smear microscopy&#44; the cumulative sensitivity values for each of the three smears were 19&#46;3&#37;&#44; 20&#46;4&#37; and 22&#46;5&#37; for direct smears and 24&#46;6&#37;&#44; 27&#46;7&#37; and 28&#46;8&#37; for concentrated samples&#46; Regarding mycobacterial culture&#44; when only one specimen was tested&#44; the sensitivities of L&#246;wenstein&#8211;Jensen and MGIT medium incubation were 33&#46;3&#37; and 43&#46;9&#37;&#46; When two samples were submitted the cumulative sensitivities increased to 37&#46;9&#37; and 51&#46;6&#37;&#44; and when three were submitted sensitivities were 41&#46;8&#37; and 55&#46;4&#37;&#46; Considering all forms of microbiological sputum analysis &#40;direct and concentrated smear&#44; solid and liquid medium culture&#41;&#44; the sensitivities for the first specimen were 51&#46;2&#37;&#46; The overall cumulative sensitivities values for the second and third sample were 59&#46;6&#37; and 63&#46;2&#37;&#46;</p><elsevierMultimedia ident="tbl0015"></elsevierMultimedia><p id="par0145" class="elsevierStylePara elsevierViewall">The same statistical analysis was only performed for patients with pulmonary tuberculosis and chest radiography with at least one cavitated lesion&#46; Compared to patients with other radiological patterns&#44; patients with cavitation had higher sensitivity values for all microbiological techniques executed&#46; The greatest sensitivity increments were observed for direct and concentrated sputum smear analysis&#44; with an average of 13&#46;0&#37; and 12&#46;4&#37; increase in sensitivity rates&#44; respectively&#46; Regarding cultural analysis&#44; the sensitivity increments were considerably lower with an average of 1&#46;1&#37; increase for L&#246;wenstein&#8211;Jensen and a 5&#46;3&#37; increase for MGIT medium incubation&#46; In this subset of patients&#44; cumulative sensitivities were also higher for all three consecutive sputum sample analysis &#40;58&#46;6&#37;&#44; 63&#46;6&#37; and 67&#46;7&#37;&#41;&#46;</p><p id="par0150" class="elsevierStylePara elsevierViewall">There was a significant increment of sensitivity rates due to additional second and third sputum specimens&#46; Sensitivity differences showed that&#44; when adding a second specimen&#44; the increment increase for direct and concentrated smear&#44; solid and liquid medium culture was 1&#46;1&#37; and 3&#46;2&#37;&#44; 4&#46;6&#37; and 7&#46;7&#37;&#44; respectively&#46; Similarly&#44; when adding a third specimen&#44; the sensitivity increase was 2&#46;1&#37; and 1&#46;1&#37;&#44; 3&#46;9&#37; and 3&#46;9&#37;&#46; The overall sensitivity difference for the two-specimen strategy was 8&#46;4&#37; and for the third-specimen strategy was 3&#46;5&#37;&#46; For patients with cavitated lesions&#44; cumulative sensitivity rates were 5&#46;1&#37; higher when adding a second&#44; and 4&#46;0&#37; higher when adding a third sputum sample&#46;</p></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Discussion</span><p id="par0155" class="elsevierStylePara elsevierViewall">The microscopic staining technique is one of the earliest methods used for detecting <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> and it remains a standard procedure for the diagnosis of tuberculosis because it is swift&#44; cheap and has a high positive predictive value&#46; On the other-hand&#44; microbiological identification of the bacilli by culture remains the <span class="elsevierStyleItalic">gold-standard</span> for the diagnosis of tuberculosis&#46;</p><p id="par0160" class="elsevierStylePara elsevierViewall">Regarding conventional microscopy&#44; the examination of concentrated sputum offered higher diagnostic yield when compared with direct smear&#46; Also&#44; there was an increase in the sensitivity values when considering two or three sputum samples rather than just one&#46; However&#44; the greatest gain was obtained when adding a second sputum sample&#44; while the third sputum sample offered a less substantial sensitivity increment&#46;</p><p id="par0165" class="elsevierStylePara elsevierViewall">Our results agree with the most consensual documented studies that show an incremental yield on more than one sputum examination<a class="elsevierStyleCrossRefs" href="#bib0225"><span class="elsevierStyleSup">19&#8211;21</span></a> and with more recent and controversial small trials claiming the relative inefficiency of the third smear and the burden it carries in undeveloped areas&#46;<a class="elsevierStyleCrossRefs" href="#bib0175"><span class="elsevierStyleSup">9&#8211;13</span></a></p><p id="par0170" class="elsevierStylePara elsevierViewall">The overall sensitivity of sputum smear microscopy&#44; culture analysis and all forms of microbiological analysis together remained low and did not exceed 28&#46;8&#37;&#44; 55&#46;4&#37; and 63&#46;2&#37;&#46; As predicted&#44; higher sensitivity rates were achieved among tuberculosis patients with pulmonary cavitation&#44; ranging from 58&#46;6&#37; for the first specimen to 67&#46;7&#37; for the third specimen&#46;</p><p id="par0175" class="elsevierStylePara elsevierViewall">Low sensitivity rates may be due to pauci-bacillary disease where only a small number of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> organisms are present in the respiratory specimens&#44; and&#47;or to technical problems&#46; During the actual decontamination and concentration process there is tuberculosis bacilli damage that can decrease the sensitivity rates of tuberculosis detection&#44; particularly in pauci-bacillary specimens&#46;</p><p id="par0180" class="elsevierStylePara elsevierViewall">One other possible explanation for a low count of bacilli disease in the tuberculosis patients observed in this study is related to the particular features of the patients observed in the PDC&#46; In fact&#44; the overwhelming majority was highly symptomatic and many had previously gone to their general physician or the hospital&#44; where they had been diagnosed with pneumonia and medicated with antibiotics such as quinolones&#46;</p><p id="par0185" class="elsevierStylePara elsevierViewall">There are also several other technical problems that could be considered&#46; First&#44; the specimen collection was done at home without any kind of medical surveillance&#46; Second&#44; all three samples were delivered to the Laboratory on the third day and only the third sample was submitted for microbiological analysis less than 24<span class="elsevierStyleHsp" style=""></span>h after being collected&#46; Third&#44; specific recommendations were made to each patient to preserve each sample in their domestic fridge&#44; but again there are no guaranties that this was in fact adhered to&#46; All of these methodological issues are directly related to the constraints of the population served by the PDC&#46; In fact&#44; many live far away and cannot afford to pay for three consecutive extra medical visits to provide adequate sputum samples&#46; This strategy was adopted once patients started failing their medical appointments and active tuberculosis cases were not being diagnosed&#46;</p><p id="par0190" class="elsevierStylePara elsevierViewall">To overcome these real-world limitations some alternative strategies could be considered&#46; Several studies have confirmed that the third examination is needless since two sputum smears are as effective as three smears for diagnosing tuberculosis<a class="elsevierStyleCrossRefs" href="#bib0225"><span class="elsevierStyleSup">19&#44;21&#8211;25</span></a> and this has correspondingly been included in the current World Health Organization recommendations on sputum examination&#46; In fact&#44; in this study&#44; the overall diagnostic sensitivity was incremented in 8&#46;4&#37; with two-specimen examination but the third-specimen added only 3&#46;5&#37; to sensitivity rates&#46; Furthermore&#44; a recent systematic review and meta-analysis showed that same-day smear microscopy is as accurate as standard microscopy stategy&#46;<a class="elsevierStyleCrossRef" href="#bib0260"><span class="elsevierStyleSup">26</span></a> So&#44; a possible alternative to standard strategies could be a same-day two-specimen examination&#44; which would diminish technical issues and reduce the number of clinical visits&#46;</p><p id="par0195" class="elsevierStylePara elsevierViewall">An additional methodological limitation needs to be addressed&#46; Sputum microscopy was performed with traditional ZN microscopy but fluorescent LED microscopy could have provided higher sensitivity rates&#46; However&#44; and despite the fact that the WHO recommends a switch to this more sensitive procedure&#44; this technique was not available at the PDC&#46;</p><p id="par0200" class="elsevierStylePara elsevierViewall">When sputum microscopy and culture are both negative&#44; clinical diagnosis of active tuberculosis may have to rely in other conventional diagnostic methods while novel diagnostic tools and further investigation may be required to enhance the identification of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> from biological specimens&#46;</p></span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Conflicts of interest</span><p id="par0205" class="elsevierStylePara elsevierViewall">The authors have no conflicts of interest to declare&#46;</p></span><span id="sec0075" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Ethical disclosures</span><span id="sec0080" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0110">Protection of human and animal subjects</span><p id="par0210" class="elsevierStylePara elsevierViewall">The authors declare that no experiments were performed on humans or animals for this study&#46;</p></span><span id="sec0085" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0115">Confidentiality of data</span><p id="par0215" class="elsevierStylePara elsevierViewall">The authors declare that they have followed the protocols of their work center on the publication of patient data&#46;</p></span><span id="sec0090" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0120">Right to privacy and informed consent</span><p id="par0220" class="elsevierStylePara elsevierViewall">The authors declare that no patient data appear in this article&#46;</p></span></span></span>"
    "textoCompletoSecciones" => array:1 [
      "secciones" => array:9 [
        0 => array:3 [
          "identificador" => "xres528853"
          "titulo" => "Abstract"
          "secciones" => array:4 [
            0 => array:2 [
              "identificador" => "abst0005"
              "titulo" => "Introduction"
            ]
            1 => array:2 [
              "identificador" => "abst0010"
              "titulo" => "Materials and methods"
            ]
            2 => array:2 [
              "identificador" => "abst0015"
              "titulo" => "Results"
            ]
            3 => array:2 [
              "identificador" => "abst0020"
              "titulo" => "Conclusions"
            ]
          ]
        ]
        1 => array:2 [
          "identificador" => "xpalclavsec549084"
          "titulo" => "Keywords"
        ]
        2 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Introduction"
        ]
        3 => array:3 [
          "identificador" => "sec0010"
          "titulo" => "Materials and methods"
          "secciones" => array:6 [
            0 => array:2 [
              "identificador" => "sec0015"
              "titulo" => "Patient selection"
            ]
            1 => array:3 [
              "identificador" => "sec0020"
              "titulo" => "Microbiology technique"
              "secciones" => array:2 [
                0 => array:2 [
                  "identificador" => "sec0025"
                  "titulo" => "Sputum collection"
                ]
                1 => array:2 [
                  "identificador" => "sec0030"
                  "titulo" => "Digestion and decontamination procedure"
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "sec0035"
              "titulo" => "Microscopy staining technique"
              "secciones" => array:1 [
                0 => array:2 [
                  "identificador" => "sec0040"
                  "titulo" => "Mycobacterial culture"
                ]
              ]
            ]
            3 => array:2 [
              "identificador" => "sec0045"
              "titulo" => "Species identification"
            ]
            4 => array:2 [
              "identificador" => "sec0050"
              "titulo" => "Antimycobacterial susceptibility testing"
            ]
            5 => array:2 [
              "identificador" => "sec0055"
              "titulo" => "Statistical analysis"
            ]
          ]
        ]
        4 => array:2 [
          "identificador" => "sec0060"
          "titulo" => "Results"
        ]
        5 => array:2 [
          "identificador" => "sec0065"
          "titulo" => "Discussion"
        ]
        6 => array:2 [
          "identificador" => "sec0070"
          "titulo" => "Conflicts of interest"
        ]
        7 => array:3 [
          "identificador" => "sec0075"
          "titulo" => "Ethical disclosures"
          "secciones" => array:3 [
            0 => array:2 [
              "identificador" => "sec0080"
              "titulo" => "Protection of human and animal subjects"
            ]
            1 => array:2 [
              "identificador" => "sec0085"
              "titulo" => "Confidentiality of data"
            ]
            2 => array:2 [
              "identificador" => "sec0090"
              "titulo" => "Right to privacy and informed consent"
            ]
          ]
        ]
        8 => array:1 [
          "titulo" => "References"
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2014-05-05"
    "fechaAceptado" => "2014-09-05"
    "PalabrasClave" => array:1 [
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec549084"
          "palabras" => array:5 [
            0 => "Tuberculosis"
            1 => "Sputum"
            2 => "Microscopy"
            3 => "Laboratory"
            4 => "<span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:1 [
      "en" => array:3 [
        "titulo" => "Abstract"
        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Introduction</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Pulmonary tuberculosis &#40;TB&#41; requires an early diagnosis for prompt introduction of treatment and prevention of transmission&#46; Definitive diagnosis is obtained by microbiological culture and identification of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> in respiratory specimens&#44; mostly sputum samples&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Materials and methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Retrospective data analysis of all patients suspected of pulmonary TB that submitted three consecutive sputum samples to the Pulmonology Diagnostic Center &#40;PDC&#41; Laboratory between 2004 and 2013&#46; Extrapulmonary TB cases were excluded&#46; Four microbiological analyses were executed on each specimen&#58; two smears with Ziehl&#8211;Neelsen staining&#44; direct and concentrate&#59; and two culture examinations&#44; one in liquid and one in solid medium&#46; Statistical analysis was performed by SPSS&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">A total of 694 patients were enrolled in this study &#40;65&#37; men&#44; mean age 48&#46;5<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>18&#46;6 years&#44; 97&#37; Portuguese&#41;&#44; most of them exhibiting TB-related complaints&#46; Pulmonary TB was diagnosed in 41&#37; of the patients&#59; 54&#37; had non-specific radiological changes and 34&#37; had pulmonary cavitation&#46; The cumulative sensitivity rates of each of the three smears were 24&#46;6&#37;&#44; 27&#46;7&#37; and 28&#46;8&#37; for concentrated samples and 19&#46;3&#37;&#44; 20&#46;4&#37; and 22&#46;5&#37; for direct samples&#46; The cumulative sensitivities of sputum culture were 33&#46;3&#37;&#44; 37&#46;9&#37; and 41&#46;8&#37; for solid medium&#44; and 43&#46;9&#37;&#44; 51&#46;6&#37; and 55&#46;4&#37; for liquid medium&#46; Pondering all forms of microbiological analysis&#44; the cumulative sensitivities of each sample were 51&#46;2&#37;&#44; 59&#46;6&#37; and 63&#46;2&#37;&#46; There was an incremental yield of 8&#46;4&#37; for the second specimen and 3&#46;5&#37; for the third specimen&#46; All sensitivity rates were higher among patients with pulmonary cavitation&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">This study showed an incremental yield with more than one sputum sample&#46; However&#44; overall sensitivity remained low&#44; suggesting a need for new diagnostic strategies and novel and better diagnostic tools&#46;</p></span>"
        "secciones" => array:4 [
          0 => array:2 [
            "identificador" => "abst0005"
            "titulo" => "Introduction"
          ]
          1 => array:2 [
            "identificador" => "abst0010"
            "titulo" => "Materials and methods"
          ]
          2 => array:2 [
            "identificador" => "abst0015"
            "titulo" => "Results"
          ]
          3 => array:2 [
            "identificador" => "abst0020"
            "titulo" => "Conclusions"
          ]
        ]
      ]
    ]
    "NotaPie" => array:1 [
      0 => array:2 [
        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Institution at which work was performed&#58; Centro de Diagn&#243;stico Pneumol&#243;gico de Coimbra&#44; Coimbra&#44; Portugal&#46; Directed by&#58; Paulo Cravo-Roxo&#44; M&#46;D&#46;</p>"
      ]
    ]
    "multimedia" => array:3 [
      0 => array:7 [
        "identificador" => "tbl0005"
        "etiqueta" => "Table 1"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:1 [
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Variable&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">No&#46;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">&#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Age&#44; mean</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">&#177;</span><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">SD years</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " colspan="2" align="char" valign="top">48&#46;5<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>18&#46;6</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Gender</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Male&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">452&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">65&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Female&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">242&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">35&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Birth origin</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Portugal&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">672&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">97&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>African country of Portuguese-spoken language&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>East Europe&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>South America&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Criteria for tuberculosis suspicion</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Passive screening &#8211; symptoms&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">631&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">91&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Contact screening&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">29&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Protocolized screening&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">27&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Other&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Pulmonary tuberculosis</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>No&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">409&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">59&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Yes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">285&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">41&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Sputum mycobacteria</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium tuberculosis</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">180&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">91&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium avium</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium fortuitum</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium gordonae</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium intracellulare</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium kansasii</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Mycobacterium genus</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab852278.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Demographic and clinical characteristics of patients suspected of pulmonary tuberculosis&#46;</p>"
        ]
      ]
      1 => array:7 [
        "identificador" => "tbl0010"
        "etiqueta" => "Table 2"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:1 [
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">No&#46;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">&#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Diagnosis confirmation technique</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Sputum analysis&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">180&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">63&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Bronchial aspirate&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">61&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">21&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Bronchoalveolar lavage&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">28&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Lung biopsy&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">16&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Radiological pattern</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Non-cavitated&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">155&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">54&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Cavitation&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">35&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Normal&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">21&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Unknown&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Comorbidities</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>No comorbidities&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">205&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">72&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Alcoholism&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">24&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Diabetes mellitus&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">18&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Neoplastic disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">14&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Intravenous drug abuse&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">12&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>HIV&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Interstitial pulmonary disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Psychiatric disorder&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pulmonary airway disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Chronic liver disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Chronic renal disease&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="3" align="left" valign="top"><span class="elsevierStyleItalic">Housing status</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Proper house&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">263&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">92&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Social community resident&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">18&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Prison inmate&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Homeless&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab852279.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Clinical characteristics of patients with pulmonary tuberculosis&#46;</p>"
        ]
      ]
      2 => array:7 [
        "identificador" => "tbl0015"
        "etiqueta" => "Table 3"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">MGIT&#44; mycobacteria growth indicator tube&#59; PPV&#44; positive predicted value&#59; NPV&#44; negative predicted value&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">True positive results&#44; <span class="elsevierStyleItalic">n</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">PPV&#44; &#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">NPV&#44; &#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Sensitivity&#44; &#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Specificity&#44; &#37;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " colspan="6" align="left" valign="top"><span class="elsevierStyleItalic">One specimen</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Direct smear&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">55&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">100&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">64&#46;0&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">19&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">100&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Concentrated smear&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">70&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">97&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">65&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">24&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>L&#246;wenstein&#8211;Jensen medium&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">95&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">96&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">68&#46;1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">33&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>MGIT&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">125&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">93&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">71&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">43&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">97&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Cumulative analysis&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">146&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">93&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">74&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">51&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">97&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="6" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="6" align="left" valign="top"><span class="elsevierStyleItalic">Two specimens</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Direct smear&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">58&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">98&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">64&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">20&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Concentrated smear&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">79&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">95&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">66&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">27&#46;7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&#46;0&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>L&#246;wenstein&#8211;Jensen medium&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">108&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">95&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">69&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">37&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">98&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>MGIT&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">147&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">90&#46;7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">74&#46;1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">51&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">96&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Cumulative analysis&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">170&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">91&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">77&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">59&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">96&#46;1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="6" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="6" align="left" valign="top"><span class="elsevierStyleItalic">Three specimens</span></td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Direct smear&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">64&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">98&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">64&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">22&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Concentrated smear&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">82&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">95&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">66&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">28&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">99&#46;0&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>L&#246;wenstein&#8211;Jensen medium&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">119&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">95&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">70&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">41&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">98&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>MGIT&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">158&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">90&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">75&#46;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">55&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">96&#46;1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Cumulative analysis&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">180&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">91&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">78&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">63&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">95&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab852277.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Diagnostic yield of sputum analysis submitted to the Pulmonology Diagnostic Centre Laboratory from patients suspected of pulmonary tuberculosis&#46;</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0005"
          "bibliografiaReferencia" => array:26 [
            0 => array:3 [
              "identificador" => "bib0135"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:1 [
                      "titulo" => "WHO &#124; Global tuberculosis report 2013"
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Libro" => array:1 [
                        "fecha" => "2013"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0140"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Large-scale use of polymerase chain reaction for detection of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> in a routine mycobacteriology laboratory"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "J&#46;E&#46; Clarridge"
                            1 => "R&#46;M&#46; Shawar"
                            2 => "T&#46;M&#46; Shinnick"
                            3 => "B&#46;B&#46; Plikaytis"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "1993"
                        "volumen" => "31"
                        "paginaInicial" => "2049"
                        "paginaFinal" => "2056"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8370729"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0145"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Evaluation of sputum smears concentrated by cytocentrifugation for detection of acid-fast bacilli"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "C&#46;A&#46; Saceanu"
                            1 => "N&#46;C&#46; Pfeiffer"
                            2 => "T&#46; McLean"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "1993"
                        "volumen" => "31"
                        "paginaInicial" => "2371"
                        "paginaFinal" => "2374"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8408558"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib0150"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Use of Gen-Probe AccuProbes to identify <span class="elsevierStyleItalic">Mycobacterium avium</span> complex&#44; <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> complex&#46; <span class="elsevierStyleItalic">Mycobacterium kansasii</span>&#44; and <span class="elsevierStyleItalic">Mycobacterium gordonae</span> directly from BACTEC TB broth cultures"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "B&#46;S&#46; Reisner"
                            1 => "A&#46;M&#46; Gatson"
                            2 => "G&#46;L&#46; Woods"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "1994"
                        "volumen" => "32"
                        "paginaInicial" => "2995"
                        "paginaFinal" => "2998"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7883888"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib0155"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Identification of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> and <span class="elsevierStyleItalic">M&#46; avium</span> complex directly from smear-positive sputum specimens and BACTEC 12B cultures by high-performance liquid chromatography with fluorescence detection and computer-driven pattern recognition models"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "K&#46;C&#46; Jost"
                            1 => "D&#46;F&#46; Dunbar"
                            2 => "S&#46;S&#46; Barth"
                            3 => "V&#46;L&#46; Headley"
                            4 => "L&#46;B&#46; Elliott"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "1995"
                        "volumen" => "33"
                        "paginaInicial" => "1270"
                        "paginaFinal" => "1277"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7615740"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib0160"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:1 [
                      "titulo" => "Manual of clinical microbiology"
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Libro" => array:3 [
                        "fecha" => "1999"
                        "paginaInicial" => "1773"
                        "editorial" => "Amer Society for Microbiology"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib0165"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Diagnostic mycobacteriology laboratory practices"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "T&#46;M&#46; Shinnick"
                            1 => "R&#46;C&#46; Good"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Clin Infect Dis"
                        "fecha" => "1995"
                        "volumen" => "21"
                        "paginaInicial" => "291"
                        "paginaFinal" => "299"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8562734"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib0170"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Tuberculosis control in resource-poor settings with high rates of HIV infection"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "K&#46;M&#46; De Cock"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Public Health"
                        "fecha" => "1996"
                        "volumen" => "86"
                        "paginaInicial" => "1071"
                        "paginaFinal" => "1073"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8712259"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib0175"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The adequacy of one sputum smear for diagnosing pulmonary tuberculosis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "B&#46; Dujardin"
                            1 => "E&#46; Haelterman"
                            2 => "W&#46; Van Damme"
                            3 => "G&#46; Kegels"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Public Health"
                        "fecha" => "1997"
                        "volumen" => "87"
                        "paginaInicial" => "1234"
                        "paginaFinal" => "1235"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9240123"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib0180"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:1 [
                  "referenciaCompleta" => "Ipuge YA&#44; Rieder HL&#44; Enarson DA&#46; The yield of acid-fast bacilli from serial smears in routine microscopy laboratories in rural Tanzania&#46; <span class="elsevierStyleItalic">Trans R Soc Trop Med Hyg</span>&#46; 90&#40;3&#41;&#58;258&#8211;261&#46;"
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib0185"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Diagnostic yield of repeated smear microscopy examinations among patients suspected of pulmonary TB in Shandong province of China"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "Z&#46;L&#46; Wu"
                            1 => "A&#46;Q&#46; Wang"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2000"
                        "volumen" => "4"
                        "paginaInicial" => "1086"
                        "paginaFinal" => "1087"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11092724"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            11 => array:3 [
              "identificador" => "bib0190"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Comparison of two versus three smears in identifying culture-positive tuberculosis patients in a rural African setting with high HIV prevalence"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "A&#46;C&#46; Crampin"
                            1 => "S&#46; Floyd"
                            2 => "F&#46; Mwaungulu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2001"
                        "volumen" => "5"
                        "paginaInicial" => "994"
                        "paginaFinal" => "999"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11716350"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib0195"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Screening tuberculosis suspects using two sputum smears"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "A&#46;D&#46; Harries"
                            1 => "N&#46;B&#46; Mphasa"
                            2 => "C&#46; Mundy"
                            3 => "A&#46; Banerjee"
                            4 => "J&#46;H&#46; Kwanjana"
                            5 => "F&#46;M&#46; Salaniponi"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2000"
                        "volumen" => "4"
                        "paginaInicial" => "36"
                        "paginaFinal" => "40"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10654642"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib0200"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Liquid vs&#46; solid culture for tuberculosis&#58; performance and cost in a resource-constrained setting"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "V&#46;N&#46; Chihota"
                            1 => "A&#46;D&#46; Grant"
                            2 => "K&#46; Fielding"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2010"
                        "volumen" => "14"
                        "paginaInicial" => "1024"
                        "paginaFinal" => "1031"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20626948"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib0205"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Time to detection of the growth of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> in MGIT 960 for determining the early bactericidal activity of antituberculosis agents"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "A&#46;H&#46; Diacon"
                            1 => "J&#46;S&#46; Maritz"
                            2 => "A&#46; Venter"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1007/s10096-010-1043-7"
                      "Revista" => array:6 [
                        "tituloSerie" => "Eur J Clin Microbiol Infect Dis"
                        "fecha" => "2010"
                        "volumen" => "29"
                        "paginaInicial" => "1561"
                        "paginaFinal" => "1565"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20820832"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            15 => array:3 [
              "identificador" => "bib0210"
              "etiqueta" => "16"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Testing of susceptibility of <span class="elsevierStyleItalic">Mycobacterium tuberculosis</span> to pyrazinamide with the nonradiometric BACTEC MGIT 960 system"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "G&#46;E&#46; Pfyffer"
                            1 => "F&#46; Palicova"
                            2 => "S&#46; R&#252;sch-Gerdes"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "2002"
                        "volumen" => "40"
                        "paginaInicial" => "1670"
                        "paginaFinal" => "1674"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11980940"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            16 => array:3 [
              "identificador" => "bib0215"
              "etiqueta" => "17"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Fluorescence versus conventional sputum smear microscopy for tuberculosis&#58; a systematic review"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "K&#46;R&#46; Steingart"
                            1 => "M&#46; Henry"
                            2 => "V&#46; Ng"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/S1473-3099(06)70578-3"
                      "Revista" => array:6 [
                        "tituloSerie" => "Lancet Infect Dis"
                        "fecha" => "2006"
                        "volumen" => "6"
                        "paginaInicial" => "570"
                        "paginaFinal" => "581"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16931408"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            17 => array:3 [
              "identificador" => "bib0220"
              "etiqueta" => "18"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Xpert<span class="elsevierStyleSup">&#174;</span> MTB&#47;RIF assay for pulmonary tuberculosis and rifampicin resistance in adults"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "K&#46;R&#46; Steingart"
                            1 => "H&#46; Sohn"
                            2 => "I&#46; Schiller"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1002/14651858.CD009593.pub2"
                      "Revista" => array:5 [
                        "tituloSerie" => "Cochrane Database Syst Rev"
                        "fecha" => "2013"
                        "volumen" => "1"
                        "paginaInicial" => "CD009593"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/24448973"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            18 => array:3 [
              "identificador" => "bib0225"
              "etiqueta" => "19"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "A method to determine the utility of the third diagnostic and the second follow-up sputum smear examinations to diagnose tuberculosis cases and failures"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "H&#46;L&#46; Rieder"
                            1 => "C&#46;Y&#46; Chiang"
                            2 => "I&#46;D&#46; Rusen"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2005"
                        "volumen" => "9"
                        "paginaInicial" => "384"
                        "paginaFinal" => "391"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15830743"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            19 => array:3 [
              "identificador" => "bib0230"
              "etiqueta" => "20"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Value of examining multiple sputum specimens in the diagnosis of pulmonary tuberculosis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "S&#46;M&#46; Nelson"
                            1 => "M&#46;A&#46; Deike"
                            2 => "C&#46;P&#46; Cartwright"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "1998"
                        "volumen" => "36"
                        "paginaInicial" => "467"
                        "paginaFinal" => "469"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9466760"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            20 => array:3 [
              "identificador" => "bib0235"
              "etiqueta" => "21"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Yield of serial sputum specimen examinations in the diagnosis of pulmonary tuberculosis&#58; a systematic review"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "S&#46;R&#46; Mase"
                            1 => "A&#46; Ramsay"
                            2 => "V&#46; Ng"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2007"
                        "volumen" => "11"
                        "paginaInicial" => "485"
                        "paginaFinal" => "495"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17439669"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            21 => array:3 [
              "identificador" => "bib0240"
              "etiqueta" => "22"
              "referencia" => array:1 [
                0 => array:1 [
                  "referenciaCompleta" => "Kumar A&#44; Bairy I&#44; Rao CR&#44; Prasad V&#44; Pattanshetty S&#44; Samarasinghe CM&#46; Adequacy of two versus three sputum specimens for the diagnosis of pulmonary tuberculosis&#46; <span class="elsevierStyleItalic">Natl Med J India</span>&#46; 22&#40;6&#41;&#58;298&#8211;299&#46;"
                ]
              ]
            ]
            22 => array:3 [
              "identificador" => "bib0245"
              "etiqueta" => "23"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Yield of two consecutive sputum specimens for the effective diagnosis of pulmonary tuberculosis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:3 [
                            0 => "M&#46;R&#46; Islam"
                            1 => "R&#46; Khatun"
                            2 => "M&#46;K&#46;M&#46; Uddin"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1371/journal.pone.0067678"
                      "Revista" => array:5 [
                        "tituloSerie" => "PLOS ONE"
                        "fecha" => "2013"
                        "volumen" => "8"
                        "paginaInicial" => "e67678"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23844057"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            23 => array:3 [
              "identificador" => "bib0250"
              "etiqueta" => "24"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Reducing the number of sputum samples examined and thresholds for positivity&#58; an opportunity to optimise smear microscopy"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "M&#46; Bonnet"
                            1 => "A&#46; Ramsay"
                            2 => "L&#46; Gagnidze"
                            3 => "W&#46; Githui"
                            4 => "P&#46;J&#46; Guerin"
                            5 => "F&#46; Varaine"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2007"
                        "volumen" => "11"
                        "paginaInicial" => "953"
                        "paginaFinal" => "958"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17705971"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            24 => array:3 [
              "identificador" => "bib0255"
              "etiqueta" => "25"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Efficiency of a third serial sputum smear examination in the diagnosis of tuberculosis in Moldova and Uganda"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "A&#46; Katamba"
                            1 => "D&#46; Laticevschi"
                            2 => "H&#46;L&#46; Rieder"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Tuberc Lung Dis"
                        "fecha" => "2007"
                        "volumen" => "11"
                        "paginaInicial" => "659"
                        "paginaFinal" => "664"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17519098"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            25 => array:3 [
              "identificador" => "bib0260"
              "etiqueta" => "26"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Diagnostic accuracy of same-day microscopy versus standard microscopy for pulmonary tuberculosis&#58; a systematic review and meta-analysis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "J&#46;L&#46; Davis"
                            1 => "A&#46; Cattamanchi"
                            2 => "L&#46;E&#46; Cuevas"
                            3 => "P&#46;C&#46; Hopewell"
                            4 => "K&#46;R&#46; Steingart"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/S1473-3099(12)70232-3"
                      "Revista" => array:6 [
                        "tituloSerie" => "Lancet Infect Dis"
                        "fecha" => "2013"
                        "volumen" => "13"
                        "paginaInicial" => "147"
                        "paginaFinal" => "154"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23099183"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/21735115/0000002100000004/v1_201507020213/S2173511515000391/v1_201507020213/en/main.assets"
  "Apartado" => array:4 [
    "identificador" => "9710"
    "tipo" => "SECCION"
    "en" => array:2 [
      "titulo" => "Original articles"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "en"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/21735115/0000002100000004/v1_201507020213/S2173511515000391/v1_201507020213/en/main.pdf?idApp=UINPBA00004E&text.app=https://journalpulmonology.org/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511515000391?idApp=UINPBA00004E"
]
Article information
ISSN: 21735115
Original language: English
The statistics are updated each day
Year/Month Html Pdf Total
2024 November 10 7 17
2024 October 79 38 117
2024 September 50 26 76
2024 August 62 31 93
2024 July 59 39 98
2024 June 54 42 96
2024 May 43 37 80
2024 April 47 42 89
2024 March 33 31 64
2024 February 28 23 51
2024 January 25 28 53
2023 December 21 27 48
2023 November 35 40 75
2023 October 32 35 67
2023 September 30 26 56
2023 August 20 12 32
2023 July 23 25 48
2023 June 22 21 43
2023 May 29 26 55
2023 April 16 11 27
Show all

Follow this link to access the full text of the article

Pulmonology

Are you a health professional able to prescribe or dispense drugs?