was read the article
array:24 [ "pii" => "S2173511516300410" "issn" => "21735115" "doi" => "10.1016/j.rppnen.2016.05.005" "estado" => "S300" "fechaPublicacion" => "2016-11-01" "aid" => "1176" "copyright" => "Sociedade Portuguesa de Pneumologia" "copyrightAnyo" => "2016" "documento" => "article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "fla" "cita" => "Rev Port Pneumol. 2016;22:315-22" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:2 [ "total" => 1545 "formatos" => array:3 [ "EPUB" => 217 "HTML" => 787 "PDF" => 541 ] ] "itemSiguiente" => array:19 [ "pii" => "S2173511516300021" "issn" => "21735115" "doi" => "10.1016/j.rppnen.2016.03.003" "estado" => "S300" "fechaPublicacion" => "2016-11-01" "aid" => "1152" "copyright" => "Sociedade Portuguesa de Pneumologia" "documento" => "article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "fla" "cita" => "Rev Port Pneumol. 2016;22:323-30" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:2 [ "total" => 2013 "formatos" => array:3 [ "EPUB" => 221 "HTML" => 1097 "PDF" => 695 ] ] "en" => array:12 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>" "titulo" => "COPD patients with severe diffusion defect in carbon monoxide diffusing capacity predict a better outcome for pulmonary rehabilitation" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "323" "paginaFinal" => "330" ] ] "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Fig. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1080 "Ancho" => 1532 "Tamanyo" => 87851 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Exercise capacity (6<span class="elsevierStyleHsp" style=""></span>minute walk test) of the patients before and after pulmonary rehabilitation (<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05 for within group change, <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.16 between groups).</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "H. Sahin, I. Naz, Y. Varol, N. Aksel, F. Tuksavul, A. Ozsoz" "autores" => array:6 [ 0 => array:2 [ "nombre" => "H." "apellidos" => "Sahin" ] 1 => array:2 [ "nombre" => "I." "apellidos" => "Naz" ] 2 => array:2 [ "nombre" => "Y." "apellidos" => "Varol" ] 3 => array:2 [ "nombre" => "N." "apellidos" => "Aksel" ] 4 => array:2 [ "nombre" => "F." "apellidos" => "Tuksavul" ] 5 => array:2 [ "nombre" => "A." "apellidos" => "Ozsoz" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511516300021?idApp=UINPBA00004E" "url" => "/21735115/0000002200000006/v1_201612110145/S2173511516300021/v1_201612110145/en/main.assets" ] "itemAnterior" => array:19 [ "pii" => "S2173511516300185" "issn" => "21735115" "doi" => "10.1016/j.rppnen.2016.03.012" "estado" => "S300" "fechaPublicacion" => "2016-11-01" "aid" => "1163" "copyright" => "Sociedade Portuguesa de Pneumologia" "documento" => "article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "fla" "cita" => "Rev Port Pneumol. 2016;22:308-14" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:2 [ "total" => 1379 "formatos" => array:3 [ "EPUB" => 199 "HTML" => 629 "PDF" => 551 ] ] "en" => array:12 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>" "titulo" => "Mid-regional proadrenomedullin: An early marker of response in critically ill patients with severe community-acquired pneumonia?" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "308" "paginaFinal" => "314" ] ] "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0020" "etiqueta" => "Figure 4" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr4.jpeg" "Alto" => 1091 "Ancho" => 1519 "Tamanyo" => 74926 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Discrimination of mid-regional proadrenomedullin kinetics in the first 48<span class="elsevierStyleHsp" style=""></span>h after antibiotic therapy, as measured by the percent decrease from baseline, to predict hospital mortality (aROC 0.80; 95% CI 0.47–1.00).</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "J.M. Pereira, A. Azevedo, C. Basílio, C. Sousa-Dias, P. Mergulhão, J.A. Paiva" "autores" => array:6 [ 0 => array:2 [ "nombre" => "J.M." "apellidos" => "Pereira" ] 1 => array:2 [ "nombre" => "A." "apellidos" => "Azevedo" ] 2 => array:2 [ "nombre" => "C." "apellidos" => "Basílio" ] 3 => array:2 [ "nombre" => "C." "apellidos" => "Sousa-Dias" ] 4 => array:2 [ "nombre" => "P." "apellidos" => "Mergulhão" ] 5 => array:2 [ "nombre" => "J.A." "apellidos" => "Paiva" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511516300185?idApp=UINPBA00004E" "url" => "/21735115/0000002200000006/v1_201612110145/S2173511516300185/v1_201612110145/en/main.assets" ] "en" => array:21 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>" "titulo" => "Acute exercise amplifies inflammation in obese patients with COPD" "tieneTextoCompleto" => true "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "315" "paginaFinal" => "322" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "F. Rodrigues, A.L. Papoila, D. Ligeiro, M.J.M. Gomes, H. Trindade" "autores" => array:5 [ 0 => array:4 [ "nombre" => "F." "apellidos" => "Rodrigues" "email" => array:1 [ 0 => "fatima.rodriguesed@gmail.com" ] "referencia" => array:3 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] 2 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">*</span>" "identificador" => "cor0005" ] ] ] 1 => array:3 [ "nombre" => "A.L." "apellidos" => "Papoila" "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">d</span>" "identificador" => "aff0020" ] ] ] 2 => array:3 [ "nombre" => "D." "apellidos" => "Ligeiro" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">e</span>" "identificador" => "aff0025" ] ] ] 3 => array:3 [ "nombre" => "M.J.M." "apellidos" => "Gomes" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] ] ] 4 => array:3 [ "nombre" => "H." "apellidos" => "Trindade" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">f</span>" "identificador" => "aff0030" ] ] ] ] "afiliaciones" => array:6 [ 0 => array:3 [ "entidad" => "Serviço Pneumologia, Hospital Pulido Valente, Centro Hospitalar Lisboa Norte, Portugal" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Faculdade de Medicina, Universidade de Lisboa, Portugal" "etiqueta" => "b" "identificador" => "aff0010" ] 2 => array:3 [ "entidad" => "NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Portugal" "etiqueta" => "c" "identificador" => "aff0015" ] 3 => array:3 [ "entidad" => "Centro de Estatística e Aplicações da Universidade de Lisboa, Portugal" "etiqueta" => "d" "identificador" => "aff0020" ] 4 => array:3 [ "entidad" => "Centro Luso-Transplante do Sul, Portugal" "etiqueta" => "e" "identificador" => "aff0025" ] 5 => array:3 [ "entidad" => "Instituto Português do Sangue e da Transplantação, Portugal" "etiqueta" => "f" "identificador" => "aff0030" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 394 "Ancho" => 2271 "Tamanyo" => 27976 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Blood sampling scheme and maximal exercise test.</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Chronic obstructive pulmonary disease (COPD) is associated with important extrapulmonary manifestations, including weight loss, skeletal muscle dysfunction, cardiovascular disease, depression, osteoporosis, reduced exercise tolerance, and poor health status.<a class="elsevierStyleCrossRefs" href="#bib0210"><span class="elsevierStyleSup">1–3</span></a> Although the pathobiology of COPD has not been fully determined, persistent systemic inflammation has been implicated in the pathogenesis of the majority of these systemic effects.<a class="elsevierStyleCrossRefs" href="#bib0220"><span class="elsevierStyleSup">3,4</span></a> Elevated circulating levels of white blood cells, C-reactive protein (CRP), interleukins 6 (IL-6) and 8 (IL-8), fibrinogen and tumor necrosis factor alpha (TNFa) have been reported in patients with COPD.<a class="elsevierStyleCrossRef" href="#bib0230"><span class="elsevierStyleSup">5</span></a> However, the ECLIPSE study<a class="elsevierStyleCrossRef" href="#bib0230"><span class="elsevierStyleSup">5</span></a> has demonstrated that a large group of patients with COPD do not suffer from systemic inflammation.</p><p id="par0010" class="elsevierStylePara elsevierViewall">The role of exercise in COPD inflammatory process has also been a matter of debate.<a class="elsevierStyleCrossRefs" href="#bib0235"><span class="elsevierStyleSup">6–15</span></a> Patients with COPD are exposed to a systemic inflammation that is amplified by exhaustive exercise. Inflammatory response to exercise is more pronounced in patients with COPD when compared to healthy controls, even at lower levels of exercise intensity.<a class="elsevierStyleCrossRefs" href="#bib0250"><span class="elsevierStyleSup">9,15</span></a> However, scientific literature on this subject is also not consensual, as several studies have shown a reduction in the level of TNFa protein expression in COPD subjects.<a class="elsevierStyleCrossRef" href="#bib0255"><span class="elsevierStyleSup">10</span></a> As pointed out by Canavan and colleagues,<a class="elsevierStyleCrossRef" href="#bib0260"><span class="elsevierStyleSup">11</span></a> some of the heterogeneity of these results might be caused by the different methods that were used in the studies (patient characterization, exercise protocols, and assay techniques). Crul and colleagues<a class="elsevierStyleCrossRef" href="#bib0265"><span class="elsevierStyleSup">12</span></a> did not find any evidence of muscle inflammation in patients with COPD, independently of whether they were in a stable or an acute exacerbation state. Conversely, others have suggested an anti-inflammatory effect of regular exercise in some low systemic inflammation chronic diseases, with beneficial outcomes in disease prevention and symptomatic improvement.<a class="elsevierStyleCrossRefs" href="#bib0235"><span class="elsevierStyleSup">6,13,14</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">In this study we aim to evaluate the inflammatory and immune regulatory gene expression profiling in peripheral blood determined at rest in patients with COPD, and the possible modification after strenuous exercise, and search for variables and patients’ characteristics associated with inflammatory expression.</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Material and methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Patients</span><p id="par0020" class="elsevierStylePara elsevierViewall">A cross-sectional study was conducted on a sample of 21 patients diagnosed with COPD, according to the Global Initiative for Obstructive Lung Disease Project (GOLD)<a class="elsevierStyleCrossRef" href="#bib0285"><span class="elsevierStyleSup">16</span></a> as post bronchodilator FEV<span class="elsevierStyleInf">1</span>/FVC<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.70. Patients were consecutively recruited on entry to a pulmonary rehabilitation (PR) program at our PR Unit, from January to December 2010. Participants were selected if clinically stable in the previous four weeks and able to exercise and to answer health status questionnaires. Patients diagnosed with other significant lung diseases, e.g. asthma, bronchiectasis or other conditions that might cause dyspnea or affect exercise performance, were excluded.</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Data collection</span><p id="par0025" class="elsevierStylePara elsevierViewall">Data collection included age, body mass index (BMI), smoking history, number of exacerbations in the previous year and comorbidities. Clinical data were obtained by interview and from medical records, including the review of concomitant medications. Charlson,<a class="elsevierStyleCrossRef" href="#bib0290"><span class="elsevierStyleSup">17</span></a> Charlson-age<a class="elsevierStyleCrossRef" href="#bib0295"><span class="elsevierStyleSup">18</span></a> and COTE (COPD specific comorbidity test)<a class="elsevierStyleCrossRef" href="#bib0300"><span class="elsevierStyleSup">19</span></a> indexes were calculated based on comorbidities data. Participants completed questionnaires on dyspnea (modified Research Council breathlessness scale and Mahler’ baseline dyspnea index),<a class="elsevierStyleCrossRef" href="#bib0305"><span class="elsevierStyleSup">20</span></a> activities of daily living (London Chest Activity of Daily Living scale – LCADL),<a class="elsevierStyleCrossRef" href="#bib0310"><span class="elsevierStyleSup">21</span></a> anxiety and depression (Hospital Anxiety and Depression scale – HADS),<a class="elsevierStyleCrossRef" href="#bib0315"><span class="elsevierStyleSup">22</span></a> and health status (St. George's Respiratory Questionnaire – SGRQ).<a class="elsevierStyleCrossRef" href="#bib0320"><span class="elsevierStyleSup">23</span></a></p><p id="par0030" class="elsevierStylePara elsevierViewall">Pulmonary function data were obtained using standardized equipment (SensorMedics Corporation, Yorba Linda, CA, USA). Post-bronchodilator spirometric values were obtained. Data were measured as absolute values (<span class="elsevierStyleItalic">L</span>) and as percent predicted of reference values.</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Exercise test and laboratory procedures</span><p id="par0035" class="elsevierStylePara elsevierViewall">Patients were subjected to an incremental exercise test to maximum tolerated on a treadmill or on a cycle ergometer (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>). Treadmill protocol started with a three minutes warming up at 2.0<span class="elsevierStyleHsp" style=""></span>km/h and 0° inclination, followed by 0.5<span class="elsevierStyleHsp" style=""></span>km/h increments per minute and 0° inclination until the patient attained a brisk walking speed without running, and then increments of 2° inclination every minute until exhaustion. Cycle ergometer protocol starts with a three minutes warm up with no added resistance, followed by 10<span class="elsevierStyleHsp" style=""></span>W of increments each minute until exhaustion. Safety criteria for terminating the exercise test were applied according to ATS/ACCP guidelines.<a class="elsevierStyleCrossRef" href="#bib0325"><span class="elsevierStyleSup">24</span></a></p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0040" class="elsevierStylePara elsevierViewall">Whole blood samples were collected from each patient at three different time points: at rest (T0), and at one hour (T1) and 24<span class="elsevierStyleHsp" style=""></span>h (T2) after the exercise test. Additionally, a blood sample from the first ten patients entering the study was collected the day before (C0), and considered as a calibration sample for relative gene expression analysis to establish the expression pattern of target genes in resting condition. These participants were told to maintain normal low-intensity activities of daily living and avoid exercising above that intensity in the previous 48<span class="elsevierStyleHsp" style=""></span>h.</p><p id="par0045" class="elsevierStylePara elsevierViewall">RNA integrity from blood cells was immediately preserved at collection with the PAXgene Blood RNA Tubes (PreAnalytiX GmbH, Hombrechtikon, Switzerland). Each sample tube was kept at room temperature for 2<span class="elsevierStyleHsp" style=""></span>h, followed by incubation at −20<span class="elsevierStyleHsp" style=""></span>°C for 24<span class="elsevierStyleHsp" style=""></span>h and thereafter stored at −80<span class="elsevierStyleHsp" style=""></span>°C. Thawed tubes were processed with the PAXgene Blood RNA Kit (PreAnalytiX GmbH) for isolation of total RNA according to manufacturers’ instructions, including DNAse digestion. Yield of purified RNA samples was evaluated with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, USA) and stored at −80<span class="elsevierStyleHsp" style=""></span>°C. Gene expression was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR), essentially as previously described.<a class="elsevierStyleCrossRef" href="#bib0330"><span class="elsevierStyleSup">25</span></a> Briefly, a template cDNA was generated by reverse transcription from 1 to 2<span class="elsevierStyleHsp" style=""></span>μg of total RNA using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA). Measurements of target genes and endogenous control (beta actin) were performed using the Taqman Gene Expressions Assays in combination with TaqMan Fast Advanced Mastermix on a 7900 HT system (Applied Biosystems), according to manufacturers’ instructions. The analyzed genes and each primer/probe assay ID were the following: interleukin 1 beta (IL-1β, Hs00174097_m1), interleukin 6 (IL-6, Hs00174131_m1), interleukin 8 (IL-8, Hs00174103_m1), inducible nitric oxide synthase (iNOS, Hs00167248_m1), interferon gamma (IFN-γ, Hs00174143_m1), tumor necrosis factor (TNF-α, Hs00174128_m1), transforming growth factor beta 1 (TGF-β1, Hs00998133_m1). The efficiency for each primer/probe assay was above 95% (as determined by the manufacturer).</p><p id="par0050" class="elsevierStylePara elsevierViewall">Endogenous gene expression was used for each assay normalization and gene expression was calculated by the adapted formula 2<span class="elsevierStyleSup">−DCt</span>*1000, which infers the number of mRNA molecules of the gene of interest per 1000 molecules of the endogenous controls.<a class="elsevierStyleCrossRef" href="#bib0335"><span class="elsevierStyleSup">26</span></a> DCt stands for the difference between the cycle threshold of the target gene and that of the endogenous control genes.</p><p id="par0055" class="elsevierStylePara elsevierViewall">A week after the maximum exercise test, patients performed a 6-minute walking test (6MWT), standardized according to international guidelines.<a class="elsevierStyleCrossRef" href="#bib0340"><span class="elsevierStyleSup">27</span></a> 6MW distance, FEV<span class="elsevierStyleInf">1</span>% predicted after bronchodilator, mMRC dyspnea scale and BMI data were aggregated to calculate BODE index.<a class="elsevierStyleCrossRef" href="#bib0345"><span class="elsevierStyleSup">28</span></a></p><p id="par0060" class="elsevierStylePara elsevierViewall">Subjects were willing and able to participate in this study and gave written informed consent prior to baseline measurements. The hospital's Ethics committee and administration board approved the trial (IRB: Study 25/07_CE/027/07), and all data were processed anonymously according to the institution's privacy policy.</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Statistical analysis</span><p id="par0065" class="elsevierStylePara elsevierViewall">Categorical data were presented as frequencies and percentages, and continuous variables as mean or median, SD or interquartile range: 25th percentile (P<span class="elsevierStyleInf">25</span>) to 75th percentile (P<span class="elsevierStyleInf">75</span>). To verify the normality assumption of parametric tests, Shapiro–Wilk goodness-of-fit test and Q–Q plots were used. To compare genetic inflammatory markers between T0 and C0, T0 and T1, and T0 and T2, nonparametric tests were used (exact Wilcoxon signed ranks test or sign test when the differences had no symmetric distributions). To identify associations between patients’ characteristics and genetic inflammatory expression in all time points, LOWESS (Locally Weighted Scatterplot Smoother), Spearman's correlation coefficient and Mann–Whitney test were applied. The significance level <span class="elsevierStyleItalic">α</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.05 was considered. Due to the exploratory nature of the study, no multiple testing procedures were used.</p><p id="par0070" class="elsevierStylePara elsevierViewall">All data were analyzed using the Statistical Package for the Social Sciences for Windows 21.0 (IBM Corp. Released 2012. IBM SPSS Statistics for Windows, Version 21.0. Armonk, NY: IBM Corp.).</p></span></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Results</span><p id="par0075" class="elsevierStylePara elsevierViewall">A total of 21 patients were recruited, agreed to participate and entered the study. There were no dropouts. Participants sample included 15 men (71.4%), with a mean age of 66.1 years (SD<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>8.27), 19 (90.5%) with smoking history (16 previous smokers – 76.2% and 3 active smokers – 14.3%), with an average 69 pack-years (range 12–150) and 2 never smokers (9.5%). These latter patients were in fact never smokers with a long-term second-hand smoke exposure. One woman was exposed for years to her husband smoking at home, and the other woman was also exposed for years to her family smoking at home and to her colleagues smoking at work. Fourteen patients (66.6%) had respiratory failure, 54.1% hypoxemic and 9.5% hypercapnic.</p><p id="par0080" class="elsevierStylePara elsevierViewall">According to GOLD categories,<a class="elsevierStyleCrossRef" href="#bib0285"><span class="elsevierStyleSup">16</span></a> 14 (67%) patients belonged to category D. Mean FEV<span class="elsevierStyleInf">1</span> was 46.76% (SD<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>20.90%,<span class="elsevierStyleHsp" style=""></span>min 21%, max 97% predicted), median BODE was 3.9 (min 0, max 8) and a median of 1.3 exacerbations (min 0, max 10) had occurred in the previous year.</p><p id="par0085" class="elsevierStylePara elsevierViewall">Mean BMI were 25.81<span class="elsevierStyleHsp" style=""></span>kg<span class="elsevierStyleHsp" style=""></span>m<span class="elsevierStyleSup">−2</span> (SD<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>4.9), ranging from 17 to 34<span class="elsevierStyleHsp" style=""></span>kg<span class="elsevierStyleHsp" style=""></span>m<span class="elsevierStyleSup">−2</span>. Three participants (14%) were malnourished, 8 (38%) were overweight, and four patients (19%) were obese (BMI 30–34<span class="elsevierStyleHsp" style=""></span>kg<span class="elsevierStyleHsp" style=""></span>m<span class="elsevierStyleSup">−2</span>). Of these latter, one had mild obstructive sleep apnea (OSA) with an Apnea Hypopnoea Index (AHI) of 7.1 events/hour, and the other had moderate obstructive sleep apnea with an Apnea Hypopnea Index (AHI) of 25.7 events/hour. Neither of these two patients were on CPAP at the time of the study. In the other two obese patients, OSA or hypoventilation were excluded based on the absence of typical night and daytime complaints, on physical examination and on normal arterial blood gases.</p><p id="par0090" class="elsevierStylePara elsevierViewall">In relation to respiratory comorbidities, tuberculous sequelae diagnosis (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>7) were made according to a history of past pulmonary tuberculosis and imagiologic features such as calcified granuloma, focal fibrosis or localized pleural thickening. None of these radiologic features were currently considered as clinically relevant. Similarly, cylindrical bronchiectasis was found in two patients, and interpreted as associated with COPD.</p><p id="par0095" class="elsevierStylePara elsevierViewall">The most prevalent comorbidities in our sample were cardiovascular (CV) disease, arterial hypertension being the most common (52.4%).</p><p id="par0100" class="elsevierStylePara elsevierViewall">Both patients with and without cardiovascular comorbidities exercised on the same ergometers (cycle and treadmill) and used the same protocols: of 15 patients with cardiovascular comorbidity, 5 exercised on cycle ergometer and 10 on treadmill. Of 6 patients without cardiovascular comorbidity, 3 exercised on cycle ergometer and 3 on treadmill.</p><p id="par0105" class="elsevierStylePara elsevierViewall">Patients without cardiovascular comorbidity achieved higher work load on cycle ergometer [mean (SD): 83 (22)<span class="elsevierStyleHsp" style=""></span>W, min–max: 70–108<span class="elsevierStyleHsp" style=""></span>W versus mean (SD): 58 (25)<span class="elsevierStyleHsp" style=""></span>W, min–max: 40–100<span class="elsevierStyleHsp" style=""></span>W] but lower workload on the treadmill [mean (SD): 5 (0.76)<span class="elsevierStyleHsp" style=""></span>km/h and 7 (6.93)° inclination, min–max: 4.5<span class="elsevierStyleHsp" style=""></span>km/h, 2.2° – 6<span class="elsevierStyleHsp" style=""></span>km/h, 12° versus mean (SD) 5 (0.96)<span class="elsevierStyleHsp" style=""></span>km/h and 10° (7.44), min–max: 4.0<span class="elsevierStyleHsp" style=""></span>km/h, 0° – 6<span class="elsevierStyleHsp" style=""></span>km/h, 22°]. All the patients exercised to their maximal tolerance.</p><p id="par0110" class="elsevierStylePara elsevierViewall">Cardiopulmonary exercise tests data are shown in Annex, Table 1a.</p><p id="par0115" class="elsevierStylePara elsevierViewall">Mean 6MWD was 346.1 meters (SD<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>86.1), ranging from 185 to 520 meters. Oximetry lowest values at 6-minute walking test were on average 85%, ranging from 68% to 94%. Baseline characteristics are presented in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>.</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0120" class="elsevierStylePara elsevierViewall">Pharmacological treatment was as follows: 18 participants were on triple therapy with long-acting muscarinic antagonist (LAMA) Tiotropium, long-acting beta-adrenergic (LABA) Salmeterol or Formoterol and inhaled corticosteroid (ICS) Budesonide, Beclometasone or Fluticasone; 2 participants were on LABA and ICS, one was on LABA, ICS and short acting muscarinic antagonist (SAMA) Ipratropium and one participant was on rescue short acting beta-adrenergic (SABA) Salbutamol, and Acetylcystein. Eight patients were on Theophylline, 4 were on statins (Simvastatin, Atorvastatin or Rosuvastatin) and 2 were on Amiodarone.</p><p id="par0125" class="elsevierStylePara elsevierViewall">Seven patients were on long-term oxygen therapy (LTOT) and one was on LTOT and non-invasive ventilation (NIV). One normal-weight patient was on CPAP due to obstructive sleep apnea.</p><p id="par0130" class="elsevierStylePara elsevierViewall">The pharmacological therapy used by the four obese patients and the other participants was essentially the same: three were on triple therapy LAMA, LABA and ICS, one was on LABA and ICS. Three were on theophylline and 2 were on statins. None of the patients were on oral corticosteroids or Roflumilast. None of the four patients were on LTOT, NIV or CPAP.</p><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Laboratory results</span><p id="par0135" class="elsevierStylePara elsevierViewall">In the first 10 patients studied, we did not find a statistically significant difference between C0 and T0, the two time points of baseline resting condition (Annex-Table 2a). The characteristics of these first ten patients entering the study were similar to all group characteristics: 6 men (60%), 4 women (40%), mean age of 63.4 (SD: 6.9) years old (ranging from 56 to 79 years), mean post-bronchodilator FEV<span class="elsevierStyleInf">1</span>%predicted 44.5% (SD: 23.6) (R: 21–85%), mean BMI 24.8<span class="elsevierStyleHsp" style=""></span>kg<span class="elsevierStyleHsp" style=""></span>m<span class="elsevierStyleSup">−2</span> (SD: 4.3) (R: 19–31<span class="elsevierStyleHsp" style=""></span>kg<span class="elsevierStyleHsp" style=""></span>m<span class="elsevierStyleSup">−2</span>), mean 69.9 pack-years (SD: 20.9) (<span class="elsevierStyleItalic">R</span>: 45–100 pack-years).</p><p id="par0140" class="elsevierStylePara elsevierViewall">In the entire sample, there was no statistically significant difference between inflammatory expression at rest and at 1<span class="elsevierStyleHsp" style=""></span>h and 24<span class="elsevierStyleHsp" style=""></span>h after the maximal exercise test (<a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>).</p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><p id="par0145" class="elsevierStylePara elsevierViewall">Looking at associations between patients’ characteristics and inflammation, the only evidence of an association was found between BMI and inflammatory expression at all time points. As presented in <a class="elsevierStyleCrossRef" href="#fig0010">Fig. 2</a>, a slightly inverse association occurs with low BMI (values under 20<span class="elsevierStyleHsp" style=""></span>kg<span class="elsevierStyleHsp" style=""></span>m<span class="elsevierStyleSup">−2</span>; <span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>3) for inflammatory genes mRNA IL1b, IL6, TNFa, TGFb and iNOS. Moreover, a more pronounced positive association was found for obese patients (BMI above 30<span class="elsevierStyleHsp" style=""></span>kg<span class="elsevierStyleHsp" style=""></span>m<span class="elsevierStyleSup">−2</span>; <span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>4) for all inflammatory mRNA genes tested, at all time points. No correlation coefficient estimates with corresponding p values were reported because only three patients were malnourished and four patients were obese.</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia><p id="par0150" class="elsevierStylePara elsevierViewall">We found no associations between inflammatory expression and all other patients’ characteristics and variables, including demographics, symptoms/health status, comorbidities and physiologic parameters (data not shown).</p><p id="par0155" class="elsevierStylePara elsevierViewall">Due to the previously reported potential influence of cardiovascular comorbidity in the inflammatory expression of COPD patients,<a class="elsevierStyleCrossRef" href="#bib0350"><span class="elsevierStyleSup">29</span></a> we looked for the association between this variable and inflammatory gene expression. Higher T1-T0 and/or T2-T0 differences were found (sometimes with statistical significance) for patients without cardiovascular comorbidity (Annex-Table 3a).</p></span></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">Discussion</span><p id="par0160" class="elsevierStylePara elsevierViewall">In our COPD patient sample, real-time polymerase chain reaction of the target mRNA inflammatory genes did not show increased inflammation either at rest or after a maximum exercise test. When looking at the inflammatory expression measured in resting samples on two consecutive days (control group), there were also no statistical differences between the two time points, which might reflect stability at rest mRNA parameters.</p><p id="par0165" class="elsevierStylePara elsevierViewall">Persistent systemic inflammation is not a universal finding in patients with COPD. ECLIPSE study recently showed that in 1755 COPD patients, about 30% do not have systemic inflammation, and only a minority (16%) have persistent inflammation during 1 year follow up.<a class="elsevierStyleCrossRef" href="#bib0230"><span class="elsevierStyleSup">5</span></a></p><p id="par0170" class="elsevierStylePara elsevierViewall">Although we expected a significantly different mRNA inflammatory expression at 1 and at 24<span class="elsevierStyleHsp" style=""></span>h after maximal exercise, in line with other authors, such as Van Helvoort et al.,<a class="elsevierStyleCrossRef" href="#bib0280"><span class="elsevierStyleSup">15</span></a> who demonstrated that COPD patients, when compared to healthy subjects, are exposed to systemic inflammation that is intensified by exhaustive exercise, we did not find a significantly different mRNA inflammatory expression after short-term exercise.</p><p id="par0175" class="elsevierStylePara elsevierViewall">However, in the subgroups of malnourished and obese COPD patients, we found opposite associations with mRNA inflammatory expression. When considering malnourished patients, mRNA levels of iNOS, IL6, IL1b, TNFa, and TGFb1 showed a tendency to decrease with increasing BMI values toward normal. Conversely, overweight and obese patients showed higher mRNA levels of TNFa, IFNg, IL1b, IL8, TGFb1, iNOS and IL6 as their BMI increasing above normal values. When considering all sample data, higher mRNA values of the malnourished on one side, and overweight and obese patients on the other, shown graphically in <a class="elsevierStyleCrossRef" href="#fig0010">Fig. 2</a>, might be blurred by the lower mRNA values of normal weight patients. This could explain the absence of a significant association of inflammation and exercise when taking into account the median values of all sample data.</p><p id="par0180" class="elsevierStylePara elsevierViewall">ECLIPSE study also evidenced high BMI as one of the independent risk factors for persistent inflammation, both at baseline and at one year of follow-up.<a class="elsevierStyleCrossRef" href="#bib0230"><span class="elsevierStyleSup">5</span></a> This association was not evident for fat free mass index, which suggests an important role for adipose tissue in systemic inflammation. Garcia-Aymerich and colleagues<a class="elsevierStyleCrossRef" href="#bib0355"><span class="elsevierStyleSup">30</span></a> also identified a “systemic” COPD subtype characterized by a higher proportion of obesity in 342 COPD patients with a significant systemic inflammation; this pattern was maintained through a 4 years’ follow-up period. In the study of Tamakoshi and colleagues, an acute phase response evidenced by serum levels of C-reactive protein increased in obesity and was associated with insulin resistance.<a class="elsevierStyleCrossRef" href="#bib0360"><span class="elsevierStyleSup">31</span></a></p><p id="par0185" class="elsevierStylePara elsevierViewall">Most of the scientific evidence has previously shown that peripheral muscle atrophy and cachexia are associated with systemic inflammation in patients with COPD, when compared to patients with no muscle wasting.<a class="elsevierStyleCrossRefs" href="#bib0365"><span class="elsevierStyleSup">32,33</span></a> In the same way, in our study, malnutrition was also associated with higher expression on mRNA genes iNOS, IL6, IL1b and TGFb1, but to a lesser extent, and in the opposite direction, when compared to what happened in patients with higher BMI.</p><p id="par0190" class="elsevierStylePara elsevierViewall">Inflammation in COPD has been associated with a worse prognosis.<a class="elsevierStyleCrossRef" href="#bib0375"><span class="elsevierStyleSup">34</span></a> In the ECLIPSE study, persistent inflamed patients had significantly increased all-cause mortality and exacerbation frequency compared with non-inflamed patients.<a class="elsevierStyleCrossRef" href="#bib0230"><span class="elsevierStyleSup">5</span></a> In our study, 8 patients were deceased after 4 years (38%), of whom, 4 (50%) were obese or overweight, and 3 (37.5%) were malnourished. Only one patient who died had had a normal BMI at baseline (data not shown).</p><p id="par0195" class="elsevierStylePara elsevierViewall">Cardiovascular disease is the most prevalent comorbidity in COPD,<a class="elsevierStyleCrossRefs" href="#bib0380"><span class="elsevierStyleSup">35–37</span></a> and it has previously been associated with systemic inflammation (elevated C-reactive protein) in patients with COPD.<a class="elsevierStyleCrossRef" href="#bib0350"><span class="elsevierStyleSup">29</span></a> However, although prevalent (71%), cardiovascular disease was not associated with higher inflammatory expression for all genes studied. These results do not seem to be influenced by different exercise protocols involved, as both patients with and without cardiovascular comorbidities exercised on the same ergometers, used the same protocols and exercised to their maximal tolerance. Patients without cardiovascular comorbidity achieved higher work load in cycle ergometer, but lower workload on the treadmill. However, assuming that the sample studied is too small to be representative, a definite conclusion of cardiovascular comorbidity not being associated with higher levels of inflammation after exercise in COPD patients can only be established with larger samples.</p><p id="par0200" class="elsevierStylePara elsevierViewall">There is still an unmet need to characterize different COPD phenotypes that might benefit from different treatment approaches and respective outcomes. Inhaled corticosteroids, as an example, have limited efficacy in the reduction of systemic inflammation in COPD.<a class="elsevierStyleCrossRef" href="#bib0395"><span class="elsevierStyleSup">38</span></a> On the other hand, a non-pharmacological approach, such as pulmonary rehabilitation with exercise training, has been proven to improve exercise tolerance, while modifying body composition toward a higher fat-free mass, improving muscle function, and, as evidenced by some studies, it might also attenuate systemic inflammation.<a class="elsevierStyleCrossRef" href="#bib0235"><span class="elsevierStyleSup">6</span></a></p><p id="par0205" class="elsevierStylePara elsevierViewall">The present study has a number of limitations that need to be addressed. A small sample size was not able to rule out the presence of an increased systemic inflammation. Although small, this sample size was enough to show graphically an association between BMI and inflammation. It is an exploratory study and longitudinal studies with larger samples are needed to confirm this association. Studies designed to definitely exclude OSA or OHS as confounding in obese patients are also required.</p><p id="par0210" class="elsevierStylePara elsevierViewall">Another limitation might be the fact that levels of plasma proteins and related mRNA precursors are sometimes not coincident.<a class="elsevierStyleCrossRefs" href="#bib0400"><span class="elsevierStyleSup">39,40</span></a> Cytokine protein production is strictly regulated at multiple steps, including transcription (mRNA chain generation) and translation (protein synthesis from RNA). Plasma cytokine expression will depend on the efficiency of translation of mRNA into useful proteins.</p><p id="par0215" class="elsevierStylePara elsevierViewall">Although our study suggested an association between obesity and several inflammatory genes, and other authors also evidenced RT-PCR as an attractive method of studying the gene expression of cytokines in whole blood,<a class="elsevierStyleCrossRef" href="#bib0410"><span class="elsevierStyleSup">41</span></a> an additional study should be carried out to confront mRNA genes and the corresponding plasma proteins.</p></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0060">Conclusions</span><p id="par0220" class="elsevierStylePara elsevierViewall">This preliminary study evidenced an association between BMI (malnutrition and obesity) and the levels of inflammatory mRNA cytokine precursors, suggesting not only malnourishment, but also obesity as potential links between COPD and systemic inflammation.</p></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Ethical disclosures</span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Protection of human and animal subjects</span><p id="par0225" class="elsevierStylePara elsevierViewall">The authors declare that no experiments were performed on humans or animals for this study.</p></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Confidentiality of data</span><p id="par0230" class="elsevierStylePara elsevierViewall">The authors declare that they have followed the protocols of their work center on the publication of patient data.</p></span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Right to privacy and informed consent</span><p id="par0235" class="elsevierStylePara elsevierViewall">The authors have obtained the written informed consent of the patients or subjects mentioned in the article. The corresponding author is in possession of this document.</p></span></span><span id="sec0075" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Conflict of interest statement</span><p id="par0240" class="elsevierStylePara elsevierViewall">None of the authors has a financial relationship with a commercial entity that has an interest in the subject of this manuscript.</p><p id="par0245" class="elsevierStylePara elsevierViewall">Acquisition of laboratory analysis kits and lab ware were partially funded by a scholarship from <span class="elsevierStyleGrantSponsor" id="gs1">Nova Medical School</span> for research projects undertaken in the affiliated hospitals of NOVA Medical School.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:11 [ 0 => array:3 [ "identificador" => "xres771343" "titulo" => "Abstract" "secciones" => array:1 [ 0 => array:1 [ "identificador" => "abst0005" ] ] ] 1 => array:2 [ "identificador" => "xpalclavsec772529" "titulo" => "Keywords" ] 2 => array:2 [ "identificador" => "sec0005" "titulo" => "Introduction" ] 3 => array:3 [ "identificador" => "sec0010" "titulo" => "Material and methods" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "sec0015" "titulo" => "Patients" ] 1 => array:2 [ "identificador" => "sec0020" "titulo" => "Data collection" ] 2 => array:2 [ "identificador" => "sec0025" "titulo" => "Exercise test and laboratory procedures" ] 3 => array:2 [ "identificador" => "sec0030" "titulo" => "Statistical analysis" ] ] ] 4 => array:3 [ "identificador" => "sec0035" "titulo" => "Results" "secciones" => array:1 [ 0 => array:2 [ "identificador" => "sec0040" "titulo" => "Laboratory results" ] ] ] 5 => array:2 [ "identificador" => "sec0045" "titulo" => "Discussion" ] 6 => array:2 [ "identificador" => "sec0050" "titulo" => "Conclusions" ] 7 => array:3 [ "identificador" => "sec0055" "titulo" => "Ethical disclosures" "secciones" => array:3 [ 0 => array:2 [ "identificador" => "sec0060" "titulo" => "Protection of human and animal subjects" ] 1 => array:2 [ "identificador" => "sec0065" "titulo" => "Confidentiality of data" ] 2 => array:2 [ "identificador" => "sec0070" "titulo" => "Right to privacy and informed consent" ] ] ] 8 => array:2 [ "identificador" => "sec0075" "titulo" => "Conflict of interest statement" ] 9 => array:2 [ "identificador" => "xack257977" "titulo" => "Acknowledgements" ] 10 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2015-10-22" "fechaAceptado" => "2016-05-11" "PalabrasClave" => array:1 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec772529" "palabras" => array:5 [ 0 => "COPD" 1 => "Inflammation" 2 => "Exercise" 3 => "Obesity" 4 => "Reverse transcriptase polymerase chain reaction" ] ] ] ] "tieneResumen" => true "resumen" => array:1 [ "en" => array:2 [ "titulo" => "Abstract" "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Systemic inflammation has been implicated in the pathogenesis of chronic obstructive pulmonary disease (COPD) systemic effects. However, most COPD patients do not suffer from persistent systemic inflammation even after exacerbations and exercise and scientific evidence has provided conflicting results. Our aim is to evaluate inflammatory gene expression at rest and at 1 and 24<span class="elsevierStyleHsp" style=""></span>h after strenuous exercise in COPD patients and study the patient variables associated with inflammatory expression.</p><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">A cross-sectional study was conducted in COPD patients who were recruited on entry to a pulmonary rehabilitation (PR) program. Demographic, clinical and functional data were collected. Blood samples were collected and gene expression was analyzed by reverse transcriptase polymerase chain reaction for IFNg, IL1b, IL6, IL8, TNFa, TGFb1 and iNOS.</p><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">The study included 21 patients (15 men, 71.4%), mean age 66.1 years old (SD<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>8.27), mean FEV<span class="elsevierStyleInf">1</span> 46.76% (SD 20.90%), 67% belonging to GOLD grade D, mean BODE index of 3.9, 90.5% with smoking history, mean BMI 25.81 (SD<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>4.87), median of 1.29 exacerbations in the previous year.</p><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">There was no statistical significant difference between inflammatory expression at rest and at 1<span class="elsevierStyleHsp" style=""></span>h and 24<span class="elsevierStyleHsp" style=""></span>h after the maximal exercise test for all tested genes.</p><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">We found an association between BMI and inflammatory expression at all the points of time checked, a slight inverse association occurs with low BMI for mRNA IL1b, IL6, TNFa, TGFb1 and iNOS, and there was a more pronounced positive association for obese patients for all tested genes.</p><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">This preliminary study did not show an enhanced inflammatory gene expression from rest to 1<span class="elsevierStyleHsp" style=""></span>h and 24<span class="elsevierStyleHsp" style=""></span>h after short-term exercise, but did show an increased inflammatory gene expression in both BMI extremes, both at rest and after exercise, suggesting not only malnourishment, but also obesity as potential links between COPD and systemic inflammation. Studies with larger samples and designed to definitely exclude OSA or OHS as confounding factors in obese patients are required.</p></span>" ] ] "NotaPie" => array:1 [ 0 => array:2 [ "etiqueta" => "☆" "nota" => "<p class="elsevierStyleNotepara" id="npar0015">Study was carried out at the Pulmonary Rehabilitation Unit, Pneumology Service, CHLN-Hospital Pulido Valente and in South Luso-Transplant Centre.</p>" ] ] "apendice" => array:1 [ 0 => array:1 [ "seccion" => array:1 [ 0 => array:4 [ "apendice" => "<p id="par0260" class="elsevierStylePara elsevierViewall">The following are the supplementary data to this article:<elsevierMultimedia ident="upi0005"></elsevierMultimedia><elsevierMultimedia ident="upi0010"></elsevierMultimedia><elsevierMultimedia ident="upi0015"></elsevierMultimedia></p>" "etiqueta" => "Appendix A" "titulo" => "Supplementary data" "identificador" => "sec0085" ] ] ] ] "multimedia" => array:7 [ 0 => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 394 "Ancho" => 2271 "Tamanyo" => 27976 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Blood sampling scheme and maximal exercise test.</p>" ] ] 1 => array:7 [ "identificador" => "fig0010" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 4202 "Ancho" => 2759 "Tamanyo" => 415004 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Association between inflammatory biomarkers at rest (T0) and at 1<span class="elsevierStyleHsp" style=""></span>h (T1) and 24<span class="elsevierStyleHsp" style=""></span>h (T2) after maximal exercise test and body mass index. A Lowess was fitted to the data. Lowess – locally weighted scatterplot smoother.</p>" ] ] 2 => array:8 [ "identificador" => "tbl0005" "etiqueta" => "Table 1" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at1" "detalle" => "Table " "rol" => "short" ] ] "tabla" => array:1 [ "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleItalic">Demographics</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="" valign="top"> \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Gender <span class="elsevierStyleItalic">m</span>/<span class="elsevierStyleItalic">w</span><span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">15 (71.4%)/6 (28.6%) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Age<span class="elsevierStyleInf">years mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">66.05 (8.27) 50–82 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>BMIkg.m−2 mean (SD) min−max \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">25.81 (4.87) 17–34 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Pack's<span class="elsevierStyleInf">mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">69.05 (30.85) 12–150 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry " colspan="2" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry " colspan="2" align="left" valign="top"><span class="elsevierStyleItalic">Symptoms/health status</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>mMRC<span class="elsevierStyleInf">median (min–max)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">1.00 (0–3) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Mahler's BDI<span class="elsevierStyleInf">median (min–max)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">7.00 (3–10) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>LCADL<span class="elsevierStyleInf">median (min–max)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">19.15 (8–36) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>HADS-anxiety<span class="elsevierStyleInf">median (min–max)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">6.19 (1–14) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>HADS-depression<span class="elsevierStyleInf">median (min–max)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">5.62 (0–12) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>SGRQ<span class="elsevierStyleInf">median (min–max)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">45.95 (27–77) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>BODE<span class="elsevierStyleInf">median (min–max)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">3.90 (0–8) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Exacerbations previous year<span class="elsevierStyleInf">median (min–max)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">1.29 (0–10) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>GOLD categories A/B/C/D<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">2 (9.5)/2 (9.5)/3 (14.3)/14 (66.6) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry " colspan="2" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry " colspan="2" align="left" valign="top"><span class="elsevierStyleItalic">Comorbidities</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>COTE<span class="elsevierStyleInf">median (min–max)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">1.24 (0–8) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Charlson<span class="elsevierStyleInf">median (min–max)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">1.62 (1–3) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Charlson-age<span class="elsevierStyleInf">median (min–max)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">3.57 (2–5) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Respiratory failure/hypoxemic/hypercapnic<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">14 (66.6)/12 (54.1)/2 (9.5) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Cardiovascular comorbidities<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">15 (71.4) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Arterial hypertension<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">11 (52.4) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Congestive heart failure/Cor pulmonale<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">5 (23.8) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Ischemic heart disease<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">2 (9.5) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Arrythmias<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">2 (9.5) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Tuberculous sequelae<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">7 (33.3) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Bronchiectasis<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">2 (9.5) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Obesity or overweight<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">12 (57.1) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Malnourished<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">3 (14.3) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Diabetes mellitus<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">1 (4.8) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Dyslipidemia<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">4 (19.0) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Osteoarticular pathology<span class="elsevierStyleInf"><span class="elsevierStyleItalic">n</span> (%)</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">4 (19.0) \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry " colspan="2" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry " colspan="2" align="left" valign="top"><span class="elsevierStyleItalic">Physiology</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>FVC<span class="elsevierStyleInf">L mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">2.75 (0.95) 0.84–5.31 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>FVC<span class="elsevierStyleInf">%mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">85.52 (23.74) 32–139 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>FEV<span class="elsevierStyleInf">1</span><span class="elsevierStyleInf"><span class="elsevierStyleItalic">L</span> median (P25-P75) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">1.02 (0.73–1.46) 0.47–3.25 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>FEV<span class="elsevierStyleInf">1% mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">46.76 (20.90) 21–97 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>FEV<span class="elsevierStyleInf">1</span>/FVC<span class="elsevierStyleInf">% mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">43.48 (14.11) 21–69 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>TLC<span class="elsevierStyleInf"><span class="elsevierStyleItalic">L</span> (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>19) mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">7.11 (1.37) 4.14–8.71 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>TLC<span class="elsevierStyleInf">% (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>19) mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">121.53 (15.64) 86–155 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>RV<span class="elsevierStyleInf"><span class="elsevierStyleItalic">L</span> (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>19) mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">4.26 (1.27) 2.51–6.43 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>RV<span class="elsevierStyleInf">% (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>19) mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">189.79 (49.19) 128–293 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>DLCO<span class="elsevierStyleInf">% (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>17) mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">45.12 (19.07) 16–95 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>KCO<span class="elsevierStyleInf">% (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>17) mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">41.41 (17.49) 13–76 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>PaO<span class="elsevierStyleInf">2 mmHg mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">65.46 (8.58) 50–77 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>PaCO<span class="elsevierStyleInf">2 mmHg median (P25-P75) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">42.10 (37.20–46.05) 35–60 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>6MWD<span class="elsevierStyleInf"><span class="elsevierStyleItalic">m</span> mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">346.05 (86.06) 185–520 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>6MWD<span class="elsevierStyleInf">%pred mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">61.00 (19.50) 29–102 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>6MWT SpO<span class="elsevierStyleInf">2% mean (SD) min–max</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">84.57 (6.67) 68–94 \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab1276936.png" ] ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Baseline characteristics of COPD participants. Spirometry values displayed are post-bronchodilator.</p>" ] ] 3 => array:8 [ "identificador" => "tbl0010" "etiqueta" => "Table 2" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at2" "detalle" => "Table " "rol" => "short" ] ] "tabla" => array:2 [ "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head " align="" valign="top" scope="col" style="border-bottom: 2px solid black"> \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">T0 \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">T1 \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">T1 vs T0<br><span class="elsevierStyleItalic">p</span> \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">T2 \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">T2 vs T0<br><span class="elsevierStyleItalic">p</span> \t\t\t\t\t\t\n \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">IFNg \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.16 (0.04–1.57) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.19 (0.06–0.95) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">1.000<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.15 (0.05–1.17) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.739<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a> \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">IL1b \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">15.46 (6.42–266.69) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">19.38 (7.39–268.48) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.383<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">23.15 (5.63–250.58) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.664<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">IL6 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.33 (0.11–2.81) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.51 (0.23–2.67) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.189<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.59 (0.10–2.05) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.815<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">IL8 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">1.82 (0.97–69.57) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">4.70 (1.87–43.26) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.189<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">2.07 (0.94–43.37) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.664<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">TNFa \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">2.99 (2.55–47.00) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">3.36 (2.25–29.61) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">1.000<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">3.42 (2.51–38.21) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.383<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">TGFb \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">199.71 (64.86–5462.36) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">224.38 (63.91–4456.02) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.383<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">373.45 (75.18–5250.38) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.078<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">iNOS \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.03 (0.01–0.29) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.04 (0.01–0.60) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.791<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.04 (0.01–0.31) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.398<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a> \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab1276937.png" ] ] ] "notaPie" => array:2 [ 0 => array:3 [ "identificador" => "tblfn0005" "etiqueta" => "a" "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Exact Wilcoxon Signed ranks test.</p>" ] 1 => array:3 [ "identificador" => "tblfn0010" "etiqueta" => "b" "nota" => "<p class="elsevierStyleNotepara" id="npar0010">Sign test.</p>" ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">Median (P<span class="elsevierStyleInf">25</span>–P<span class="elsevierStyleInf">75</span>) of the mRNA inflammatory genes at rest (T0), and at 1<span class="elsevierStyleHsp" style=""></span>h (T1) and 24<span class="elsevierStyleHsp" style=""></span>h (T2) after maximal exercise test. Comparison of T1 versus T0 and T2 versus T0.</p>" ] ] 4 => array:5 [ "identificador" => "upi0005" "tipo" => "MULTIMEDIAECOMPONENTE" "mostrarFloat" => false "mostrarDisplay" => true "Ecomponente" => array:2 [ "fichero" => "mmc1.docx" "ficheroTamanyo" => 15392 ] ] 5 => array:5 [ "identificador" => "upi0010" "tipo" => "MULTIMEDIAECOMPONENTE" "mostrarFloat" => false "mostrarDisplay" => true "Ecomponente" => array:2 [ "fichero" => "mmc2.docx" "ficheroTamanyo" => 13310 ] ] 6 => array:5 [ "identificador" => "upi0015" "tipo" => "MULTIMEDIAECOMPONENTE" "mostrarFloat" => false "mostrarDisplay" => true "Ecomponente" => array:2 [ "fichero" => "mmc3.docx" "ficheroTamanyo" => 12863 ] ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:41 [ 0 => array:3 [ "identificador" => "bib0210" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Systemic manifestations and comorbidities of COPD" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "P.J. Barnes" 1 => "B.R. Celli" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1183/09031936.00128008" "Revista" => array:6 [ "tituloSerie" => "Eur Respir J" "fecha" => "2009" "volumen" => "33" "paginaInicial" => "1165" "paginaFinal" => "1185" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19407051" "web" => "Medline" ] ] ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib0215" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Systemic inflammation in chronic obstructive pulmonary disease: a population-based study" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "F. Garcia-Rio" 1 => "M. Miravitlles" 2 => "J.B. Soriano" 3 => "L. Munoz" 4 => "E. Duran-Tauleria" 5 => "G. Sanchez" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1186/1465-9921-11-63" "Revista" => array:5 [ "tituloSerie" => "Respir Res" "fecha" => "2010" "volumen" => "11" "paginaInicial" => "63" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20500811" "web" => "Medline" ] ] ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib0220" "etiqueta" => "3" "referencia" => array:1 [ 0 => array:3 [ "comentario" => "[discussion 371–2]" "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Systemic effects of chronic obstructive pulmonary disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "A.G. Agusti" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1513/pats.200504-026SR" "Revista" => array:6 [ "tituloSerie" => "Proc Am Thorac Soc" "fecha" => "2005" "volumen" => "2" "paginaInicial" => "367" "paginaFinal" => "370" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16267364" "web" => "Medline" ] ] ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib0225" "etiqueta" => "4" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Association between chronic obstructive pulmonary disease and systemic inflammation: a systematic review and a meta-analysis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "W.Q. Gan" 1 => "S.F. Man" 2 => "A. Senthilselvan" 3 => "D.D. Sin" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Thorax" "fecha" => "2004" "volumen" => "59" "paginaInicial" => "574" "paginaFinal" => "580" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15223864" "web" => "Medline" ] ] ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib0230" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Persistent systemic inflammation is associated with poor clinical outcomes in COPD: a novel phenotype" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "A. Agusti" 1 => "L.D. Edwards" 2 => "S.I. Rennard" 3 => "W. MacNee" 4 => "R. Tal-Singer" 5 => "B.E. Miller" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1371/journal.pone.0037483" "Revista" => array:5 [ "tituloSerie" => "PLoS ONE" "fecha" => "2012" "volumen" => "7" "paginaInicial" => "e37483" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22624038" "web" => "Medline" ] ] ] ] ] ] ] ] 5 => array:3 [ "identificador" => "bib0235" "etiqueta" => "6" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The potential anti-inflammatory effect of exercise in chronic obstructive pulmonary disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "J. van der Vlist" 1 => "T.W. Janssen" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1159/000233264" "Revista" => array:6 [ "tituloSerie" => "Respiration" "fecha" => "2010" "volumen" => "79" "paginaInicial" => "160" "paginaFinal" => "174" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19672044" "web" => "Medline" ] ] ] ] ] ] ] ] 6 => array:3 [ "identificador" => "bib0240" "etiqueta" => "7" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Exercise-induced systemic effects in muscle-wasted patients with COPD" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "H.A. Van Helvoort" 1 => "Y.F. Heijdra" 2 => "H.M. Thijs" 3 => "J. Vina" 4 => "G.J. Wanten" 5 => "P.N. Dekhuijzen" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1249/01.mss.0000228331.13123.53" "Revista" => array:6 [ "tituloSerie" => "Med Sci Sports Exerc" "fecha" => "2006" "volumen" => "38" "paginaInicial" => "1543" "paginaFinal" => "1552" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16960513" "web" => "Medline" ] ] ] ] ] ] ] ] 7 => array:3 [ "identificador" => "bib0245" "etiqueta" => "8" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Systemic immunological response to exercise in patients with chronic obstructive pulmonary disease: what does it mean?" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "H.A. van Helvoort" 1 => "Y.F. Heijdra" 2 => "P.N. Dekhuijzen" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1159/000091117" "Revista" => array:6 [ "tituloSerie" => "Respiration" "fecha" => "2006" "volumen" => "73" "paginaInicial" => "255" "paginaFinal" => "264" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16432297" "web" => "Medline" ] ] ] ] ] ] ] ] 8 => array:3 [ "identificador" => "bib0250" "etiqueta" => "9" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Increased tumour necrosis factor-alpha plasma levels during moderate-intensity exercise in COPD patients" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "R.A. Rabinovich" 1 => "M. Figueras" 2 => "E. Ardite" 3 => "N. Carbo" 4 => "T. Troosters" 5 => "X. Filella" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Eur Respir J" "fecha" => "2003" "volumen" => "21" "paginaInicial" => "789" "paginaFinal" => "794" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12765422" "web" => "Medline" ] ] ] ] ] ] ] ] 9 => array:3 [ "identificador" => "bib0255" "etiqueta" => "10" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Cytokine profile in quadriceps muscles of patients with severe COPD" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "E. Barreiro" 1 => "A.M. Schols" 2 => "M.I. Polkey" 3 => "J.B. Galdiz" 4 => "H.R. Gosker" 5 => "E.B. Swallow" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1136/thx.2007.078030" "Revista" => array:6 [ "tituloSerie" => "Thorax" "fecha" => "2008" "volumen" => "63" "paginaInicial" => "100" "paginaFinal" => "107" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17875568" "web" => "Medline" ] ] ] ] ] ] ] ] 10 => array:3 [ "identificador" => "bib0260" "etiqueta" => "11" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Acute exercise testing of COPD patients: the effect on systemic inflammatory proteins" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "J. Canavan" 1 => "B. Linton-Willoughby" 2 => "R. Garrod" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Curr Res Med Rev" "fecha" => "2011" "volumen" => "7" "paginaInicial" => "464" "paginaFinal" => "474" ] ] ] ] ] ] 11 => array:3 [ "identificador" => "bib0265" "etiqueta" => "12" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Markers of inflammation and disuse in vastus lateralis of chronic obstructive pulmonary disease patients" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "T. Crul" 1 => "M.A. Spruit" 2 => "G. Gayan-Ramirez" 3 => "R. Quarck" 4 => "R. Gosselink" 5 => "T. Troosters" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/j.1365-2362.2007.01867.x" "Revista" => array:6 [ "tituloSerie" => "Eur J Clin Invest" "fecha" => "2007" "volumen" => "37" "paginaInicial" => "897" "paginaFinal" => "904" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17883420" "web" => "Medline" ] ] ] ] ] ] ] ] 12 => array:3 [ "identificador" => "bib0270" "etiqueta" => "13" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Physical activity and modulation of systemic low-level inflammation" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "H. Bruunsgaard" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1189/jlb.0505247" "Revista" => array:6 [ "tituloSerie" => "J Leukoc Biol" "fecha" => "2005" "volumen" => "78" "paginaInicial" => "819" "paginaFinal" => "835" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16033812" "web" => "Medline" ] ] ] ] ] ] ] ] 13 => array:3 [ "identificador" => "bib0275" "etiqueta" => "14" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The role of IL-6 in mediating the anti-inflammatory effects of exercise" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "A.M. Petersen" 1 => "B.K. Pedersen" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:7 [ "tituloSerie" => "J Physiol Pharmacol" "fecha" => "2006" "volumen" => "57" "numero" => "Suppl." "paginaInicial" => "43" "paginaFinal" => "51" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17242490" "web" => "Medline" ] ] ] ] ] ] ] ] 14 => array:3 [ "identificador" => "bib0280" "etiqueta" => "15" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Systemic inflammatory response to exhaustive exercise in patients with chronic obstructive pulmonary disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "H.A. van Helvoort" 1 => "M.H. van de Pol" 2 => "Y.F. Heijdra" 3 => "P.N. Dekhuijzen" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.rmed.2005.03.028" "Revista" => array:6 [ "tituloSerie" => "Respir Med" "fecha" => "2005" "volumen" => "99" "paginaInicial" => "1555" "paginaFinal" => "1567" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15890510" "web" => "Medline" ] ] ] ] ] ] ] ] 15 => array:3 [ "identificador" => "bib0285" "etiqueta" => "16" "referencia" => array:1 [ 0 => array:3 [ "comentario" => "Available from" "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "GOLD (Global Initiative for Chronic Obstructive Lung Disease)" ] ] ] ] ] "host" => array:2 [ 0 => array:1 [ "Libro" => array:1 [ "fecha" => "2015" ] ] 1 => array:1 [ "WWW" => array:1 [ "link" => "http://www.goldcopd.org" ] ] ] ] ] ] 16 => array:3 [ "identificador" => "bib0290" "etiqueta" => "17" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "A new method of classifying prognostic comorbidity in longitudinal studies: development and validation" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "M.E. Charlson" 1 => "P. Pompei" 2 => "K.L. Ales" 3 => "C.R. MacKenzie" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "J Chronic Dis" "fecha" => "1987" "volumen" => "40" "paginaInicial" => "373" "paginaFinal" => "383" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/3558716" "web" => "Medline" ] ] ] ] ] ] ] ] 17 => array:3 [ "identificador" => "bib0295" "etiqueta" => "18" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Validation of a combined comorbidity index" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "M. Charlson" 1 => "T.P. Szatrowski" 2 => "J. Peterson" 3 => "J. Gold" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "J Clin Epidemiol" "fecha" => "1994" "volumen" => "47" "paginaInicial" => "1245" "paginaFinal" => "1251" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7722560" "web" => "Medline" ] ] ] ] ] ] ] ] 18 => array:3 [ "identificador" => "bib0300" "etiqueta" => "19" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Comorbidities and risk of mortality in patients with chronic obstructive pulmonary disease" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "M. Divo" 1 => "C. Cote" 2 => "J.P. de Torres" 3 => "C. Casanova" 4 => "J.M. Marin" 5 => "V. Pinto-Plata" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1164/rccm.201201-0034OC" "Revista" => array:6 [ "tituloSerie" => "Am J Respir Crit Care Med" "fecha" => "2012" "volumen" => "186" "paginaInicial" => "155" "paginaFinal" => "161" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22561964" "web" => "Medline" ] ] ] ] ] ] ] ] 19 => array:3 [ "identificador" => "bib0305" "etiqueta" => "20" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Evaluation of clinical methods for rating dyspnea" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "D.A. Mahler" 1 => "C.K. Wells" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Chest" "fecha" => "1988" "volumen" => "93" "paginaInicial" => "580" "paginaFinal" => "586" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/3342669" "web" => "Medline" ] ] ] ] ] ] ] ] 20 => array:3 [ "identificador" => "bib0310" "etiqueta" => "21" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Development and validation of a standardized measure of activity of daily living in patients with severe COPD: the London Chest Activity of Daily Living scale (LCADL)" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "R. Garrod" 1 => "J.C. Bestall" 2 => "E.A. Paul" 3 => "J.A. Wedzicha" 4 => "P.W. Jones" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1053/rmed.2000.0786" "Revista" => array:7 [ "tituloSerie" => "Respir Med" "fecha" => "2000" "volumen" => "94" "paginaInicial" => "589" "paginaFinal" => "596" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10921765" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S1063458411000471" "estado" => "S300" "issn" => "10634584" ] ] ] ] ] ] ] 21 => array:3 [ "identificador" => "bib0315" "etiqueta" => "22" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The hospital anxiety and depression scale" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "A.S. Zigmond" 1 => "R.P. Snaith" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Acta Psychiatr Scand" "fecha" => "1983" "volumen" => "67" "paginaInicial" => "361" "paginaFinal" => "370" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/6880820" "web" => "Medline" ] ] ] ] ] ] ] ] 22 => array:3 [ "identificador" => "bib0320" "etiqueta" => "23" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "A self-complete measure of health status for chronic airflow limitation. The St. George's Respiratory Questionnaire" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "P.W. Jones" 1 => "F.H. Quirk" 2 => "C.M. Baveystock" 3 => "P. Littlejohns" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1164/ajrccm/145.6.1321" "Revista" => array:6 [ "tituloSerie" => "Am Rev Respir Dis" "fecha" => "1992" "volumen" => "145" "paginaInicial" => "1321" "paginaFinal" => "1327" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/1595997" "web" => "Medline" ] ] ] ] ] ] ] ] 23 => array:3 [ "identificador" => "bib0325" "etiqueta" => "24" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Statement on cardiopulmonary exercise testing" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "ATS/ACCP" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1164/ajrccm.156.1.9605044" "Revista" => array:5 [ "tituloSerie" => "Am J Respir Crit Care Med" "fecha" => "2003" "paginaInicial" => "211" "paginaFinal" => "277" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9230750" "web" => "Medline" ] ] ] ] ] ] ] ] 24 => array:3 [ "identificador" => "bib0330" "etiqueta" => "25" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Surface alpha 2-3- and alpha 2-6-sialylation of human monocytes and derived dendritic cells and its influence on endocytosis" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "P.A. Videira" 1 => "I.F. Amado" 2 => "H.J. Crespo" 3 => "M.C. Alguero" 4 => "F. Dall’Olio" 5 => "M.G. Cabral" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1007/s10719-007-9092-6" "Revista" => array:7 [ "tituloSerie" => "Glycoconj J" "fecha" => "2008" "volumen" => "25" "paginaInicial" => "259" "paginaFinal" => "268" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18080182" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S1063458412008564" "estado" => "S300" "issn" => "10634584" ] ] ] ] ] ] ] 25 => array:3 [ "identificador" => "bib0335" "etiqueta" => "26" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "A novel method to compensate for different amplification efficiencies between patient DNA samples in quantitative real-time PCR" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "J. Meijerink" 1 => "C. Mandigers" 2 => "L. van de Locht" 3 => "E. Tonnissen" 4 => "F. Goodsaid" 5 => "J. Raemaekers" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/S1525-1578(10)60652-6" "Revista" => array:6 [ "tituloSerie" => "J Mol Diagn" "fecha" => "2001" "volumen" => "3" "paginaInicial" => "55" "paginaFinal" => "61" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11333300" "web" => "Medline" ] ] ] ] ] ] ] ] 26 => array:3 [ "identificador" => "bib0340" "etiqueta" => "27" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:1 [ "titulo" => "ATS statement: guidelines for the six-minute walk test" ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1164/ajrccm.166.1.at1102" "Revista" => array:6 [ "tituloSerie" => "Am J Respir Crit Care Med" "fecha" => "2002" "volumen" => "166" "paginaInicial" => "111" "paginaFinal" => "117" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12091180" "web" => "Medline" ] ] ] ] ] ] ] ] 27 => array:3 [ "identificador" => "bib0345" "etiqueta" => "28" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The body-mass index, airflow obstruction, dyspnea, and exercise capacity index in chronic obstructive pulmonary disease" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "B.R. Celli" 1 => "C.G. Cote" 2 => "J.M. Marin" 3 => "C. Casanova" 4 => "M. Montes de Oca" 5 => "R.A. Mendez" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1056/NEJMoa021322" "Revista" => array:6 [ "tituloSerie" => "N Engl J Med" "fecha" => "2004" "volumen" => "350" "paginaInicial" => "1005" "paginaFinal" => "1012" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/14999112" "web" => "Medline" ] ] ] ] ] ] ] ] 28 => array:3 [ "identificador" => "bib0350" "etiqueta" => "29" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Why are patients with chronic obstructive pulmonary disease at increased risk of cardiovascular diseases? The potential role of systemic inflammation in chronic obstructive pulmonary disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "D.D. Sin" 1 => "S.F. Man" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Circulation" "fecha" => "2003" "volumen" => "107" "paginaInicial" => "1514" "paginaFinal" => "1519" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12654609" "web" => "Medline" ] ] ] ] ] ] ] ] 29 => array:3 [ "identificador" => "bib0355" "etiqueta" => "30" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Identification and prospective validation of clinically relevant chronic obstructive pulmonary disease (COPD) subtypes" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "J. Garcia-Aymerich" 1 => "F.P. Gomez" 2 => "M. Benet" 3 => "E. Farrero" 4 => "X. Basagana" 5 => "A. Gayete" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1136/thx.2010.154484" "Revista" => array:6 [ "tituloSerie" => "Thorax" "fecha" => "2011" "volumen" => "66" "paginaInicial" => "430" "paginaFinal" => "437" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/21177668" "web" => "Medline" ] ] ] ] ] ] ] ] 30 => array:3 [ "identificador" => "bib0360" "etiqueta" => "31" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The metabolic syndrome is associated with elevated circulating C-reactive protein in healthy reference range: a systemic low-grade inflammatory state" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "K. Tamakoshi" 1 => "H. Yatsuya" 2 => "T. Kondo" 3 => "Y. Hori" 4 => "M. Ishikawa" 5 => "H. Zhang" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1038/sj.ijo.0802260" "Revista" => array:6 [ "tituloSerie" => "Int J Obes Relat Metab Disord" "fecha" => "2003" "volumen" => "27" "paginaInicial" => "443" "paginaFinal" => "449" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12664077" "web" => "Medline" ] ] ] ] ] ] ] ] 31 => array:3 [ "identificador" => "bib0365" "etiqueta" => "32" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Peripheral muscle wasting in chronic obstructive pulmonary disease. Clinical relevance and mechanisms" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "R. Debigare" 1 => "C.H. Cote" 2 => "F. Maltais" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1164/ajrccm.164.9.2104035" "Revista" => array:6 [ "tituloSerie" => "Am J Respir Crit Care Med" "fecha" => "2001" "volumen" => "164" "paginaInicial" => "1712" "paginaFinal" => "1717" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11719314" "web" => "Medline" ] ] ] ] ] ] ] ] 32 => array:3 [ "identificador" => "bib0370" "etiqueta" => "33" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Evidence for a relation between metabolic derangements and increased levels of inflammatory mediators in a subgroup of patients with chronic obstructive pulmonary disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "A.M. Schols" 1 => "W.A. Buurman" 2 => "A.J. Staal van den Brekel" 3 => "M.A. Dentener" 4 => "E.F. Wouters" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Thorax" "fecha" => "1996" "volumen" => "51" "paginaInicial" => "819" "paginaFinal" => "824" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8795671" "web" => "Medline" ] ] ] ] ] ] ] ] 33 => array:3 [ "identificador" => "bib0375" "etiqueta" => "34" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "[Prognostic factors in chronic obstructive pulmonary disease]" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "J.J. Soler Cataluna" 1 => "M.A. Martinez Garcia" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Arch Bronconeumol" "fecha" => "2007" "volumen" => "43" "paginaInicial" => "680" "paginaFinal" => "691" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18053546" "web" => "Medline" ] ] ] ] ] ] ] ] 34 => array:3 [ "identificador" => "bib0380" "etiqueta" => "35" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Patterns of comorbidities in newly diagnosed COPD and asthma in primary care" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "J.B. Soriano" 1 => "G.T. Visick" 2 => "H. Muellerova" 3 => "N. Payvandi" 4 => "A.L. Hansell" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1378/chest.128.4.2099" "Revista" => array:6 [ "tituloSerie" => "Chest" "fecha" => "2005" "volumen" => "128" "paginaInicial" => "2099" "paginaFinal" => "2107" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16236861" "web" => "Medline" ] ] ] ] ] ] ] ] 35 => array:3 [ "identificador" => "bib0385" "etiqueta" => "36" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Impact of comorbidities in pulmonary rehabilitation outcomes in patients with chronic obstructive pulmonary disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "A. Carreiro" 1 => "J. Santos" 2 => "F. Rodrigues" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.rppneu.2012.12.004" "Revista" => array:6 [ "tituloSerie" => "Rev Port Pneumol" "fecha" => "2013" "volumen" => "19" "paginaInicial" => "106" "paginaFinal" => "113" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23664247" "web" => "Medline" ] ] ] ] ] ] ] ] 36 => array:3 [ "identificador" => "bib0390" "etiqueta" => "37" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Co-morbidities in patients with gold stage 4 chronic obstructive pulmonary disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "V. Areias" 1 => "S. Carreira" 2 => "M. Anciaes" 3 => "P. Pinto" 4 => "C. Barbara" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.rppneu.2013.02.004" "Revista" => array:7 [ "tituloSerie" => "Rev Port Pneumol" "fecha" => "2014" "volumen" => "20" "paginaInicial" => "5" "paginaFinal" => "11" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23993405" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S1063458414010668" "estado" => "S300" "issn" => "10634584" ] ] ] ] ] ] ] 37 => array:3 [ "identificador" => "bib0395" "etiqueta" => "38" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The effects of fluticasone with or without salmeterol on systemic biomarkers of inflammation in chronic obstructive pulmonary disease" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "D.D. Sin" 1 => "S.F. Man" 2 => "D.D. Marciniuk" 3 => "G. Ford" 4 => "M. FitzGerald" 5 => "E. Wong" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1164/rccm.200709-1356OC" "Revista" => array:6 [ "tituloSerie" => "Am J Respir Crit Care Med" "fecha" => "2008" "volumen" => "177" "paginaInicial" => "1207" "paginaFinal" => "1214" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18310480" "web" => "Medline" ] ] ] ] ] ] ] ] 38 => array:3 [ "identificador" => "bib0400" "etiqueta" => "39" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Exercise elevates plasma levels but not gene expression of IL-1beta: IL-6, and TNF-alpha in blood mononuclear cells" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "A.I. Moldoveanu" 1 => "R.J. Shephard" 2 => "P.N. Shek" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "J Appl Physiol (1985)" "fecha" => "2000" "volumen" => "89" "paginaInicial" => "1499" "paginaFinal" => "1504" ] ] ] ] ] ] 39 => array:3 [ "identificador" => "bib0405" "etiqueta" => "40" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Evidence that interleukin-6 is produced in human skeletal muscle during prolonged running" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "K. Ostrowski" 1 => "T. Rohde" 2 => "M. Zacho" 3 => "S. Asp" 4 => "B.K. Pedersen" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:8 [ "tituloSerie" => "J Physiol" "fecha" => "1998" "volumen" => "508" "numero" => "Pt 3" "paginaInicial" => "949" "paginaFinal" => "953" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9518745" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S1063458414013429" "estado" => "S300" "issn" => "10634584" ] ] ] ] ] ] ] 40 => array:3 [ "identificador" => "bib0410" "etiqueta" => "41" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "A semi-quantitative reverse transcriptase polymerase chain reaction method for measurement of MRNA for TNF-alpha and IL-1 beta in whole blood cultures: its application in typhoid fever and exentric exercise" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "M.G. Netea" 1 => "J.P. Drenth" 2 => "N. De Bont" 3 => "A. Hijmans" 4 => "M. Keuter" 5 => "E. Dharmana" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1006/cyto.1996.0098" "Revista" => array:6 [ "tituloSerie" => "Cytokine" "fecha" => "1996" "volumen" => "8" "paginaInicial" => "739" "paginaFinal" => "744" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8932986" "web" => "Medline" ] ] ] ] ] ] ] ] ] ] ] ] "agradecimientos" => array:1 [ 0 => array:4 [ "identificador" => "xack257977" "titulo" => "Acknowledgements" "texto" => "<p id="par0250" class="elsevierStylePara elsevierViewall">We would like to thank Eunice Silva for her help in collecting blood samples and performing laboratory analysis.</p>" "vista" => "all" ] ] ] "idiomaDefecto" => "en" "url" => "/21735115/0000002200000006/v1_201612110145/S2173511516300410/v1_201612110145/en/main.assets" "Apartado" => array:4 [ "identificador" => "9710" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "Original articles" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/21735115/0000002200000006/v1_201612110145/S2173511516300410/v1_201612110145/en/main.pdf?idApp=UINPBA00004E&text.app=https://journalpulmonology.org/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173511516300410?idApp=UINPBA00004E" ]
Year/Month | Html | Total | |
---|---|---|---|
2024 November | 11 | 6 | 17 |
2024 October | 24 | 37 | 61 |
2024 September | 29 | 24 | 53 |
2024 August | 39 | 41 | 80 |
2024 July | 22 | 29 | 51 |
2024 June | 22 | 18 | 40 |
2024 May | 36 | 31 | 67 |
2024 April | 20 | 28 | 48 |
2024 March | 31 | 17 | 48 |
2024 February | 28 | 24 | 52 |
2024 January | 15 | 23 | 38 |
2023 December | 21 | 25 | 46 |
2023 November | 28 | 38 | 66 |
2023 October | 20 | 37 | 57 |
2023 September | 44 | 38 | 82 |
2023 August | 13 | 17 | 30 |
2023 July | 19 | 23 | 42 |
2023 June | 24 | 14 | 38 |
2023 May | 28 | 22 | 50 |
2023 April | 17 | 8 | 25 |